Ingestion of Lactobacillus strain reduces anxiety and improves cognitive function in the hyperammonemia rat
Open accessAbstract
Evidence suggests that the hyperammonemia (HA)-induced neuroinflammation and alterations in the serotonin (5-HT) system may contribute to cognitive decline and anxiety disorder during hepatic encephalopathy (HE). Probiotics that maintain immune system homeostasis and regulate the 5-HT system may be potential treatment for HA-mediated neurological disorders in HE. In this study, we tested the efficacy of probiotic Lactobacillus helveticus strain NS8 in preventing cognitive decline and anxiety-like behavior in HA rats. Chronic HA was induced by intraperitoneal injection of ammonium acetate for four weeks in male Sprague-Dawley rats. HA rats were then given Lactobacillus helveticus strain NS8 (10(9) CFU mL(-1)) in drinking water as a daily supplementation. The Morris water maze task assessed cognitive function, and the elevated plus maze test evaluated anxiety-like behavior. Neuroinflammation was assessed by measuring the inflammatory markers: inducible nitric oxide synthase, prostaglandin E2, and interleukin-1 β in the brain. 5-HT system activity was evaluated by measuring 5-HT and its metabolite, 5-HIAA, and the 5-HT precursor, tryptophan. Probiotic treatment of HA rats significantly reduced the level of inflammatory markers, decreased 5-HT metabolism, restored cognitive function and improved anxiety-like behavior. These results indicate that probiotic L. helveticus strain NS8 is beneficial for the treatment of cognitive decline and anxiety-like behavior in HA rats.
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Lactobacillus strains restore spatial memory impairments induced by diet in immunodeficient mice, age, hyperammonemia, and gut microbiota depletion after chronic antibiotic treatment.
“These strains also proved effective in restoring spatial memory impairments induced by diet in immunodeficient mice (Ohland et al., 2013), age (Jeong et al., 2015), hyperammonemia (Luo et al., 2014), and by gut microbiota depletion after chronic antibiotic treatment (Wang et al., 2015).”
From The Microbiota-Gut-Brain Axis (2019) · cites this paper as Luo et al., 2014
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- Authors
- Luo, J., Wang, T., Liang, S., Hu, X., Li, W., & Jin, F.
- Affiliations
- Chinese Academy of Sciences (CN)
- Corresponding author
- Jin, Feng
- Venue
- Science China Life Sciences
- Published
- 2014-02-19
- Volume
- 57
- Issue
- 3
- Pages
- 327-335
- ISSN
- 1674-7305 (ISSN-L)
- 1869-1889
- OpenAlex
- W2063894277
- Cited by
- 187
- References
- 4
- Citations by year
- 2026: 11 · 2025: 12 · 2024: 12 · 2023: 12 · 2022: 29 · 2021: 19 · 2020: 29 · 2019: 13 · 2018: 22 · 2017: 9 · 2016: 11 · 2015: 7 · 2014: 1
- FWCI
- 13.37
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Science China Life Sciences (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Neuroscience › Biological Psychiatry › Tryptophan and brain disorders
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- hyperammonemia, probiotics, hepatic encephalopathy, anxiety-like behavior, cognitive function, neuroinflammation, serotonergic system, 5-hydroxyindoleacetic acid, tryptophan, indoleamine 2,3-dioxygenase, IL-1β, prostaglandin E2, inducible nitric oxide synthase, cognitive decline
- MeSH terms
- Animals, Anxiety, Brain, Cognition Disorders, Disease Models, Animal, Enzyme-Linked Immunosorbent Assay, Hepatic Encephalopathy, Humans, Hydroxyindoleacetic Acid, Lactobacillus, Male, Serotonin, Dinoprostone, Rats, Sprague-Dawley, Inflammation Mediators, Probiotics, Hyperammonemia, Rats, Nitric Oxide Synthase Type II, Interleukin-1beta
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