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IL-17 Exerts Anti-Apoptotic Effect via miR-155-5p Downregulation in Experimental Autoimmune Encephalomyelitis

Ksiazek-Winiarek, Dominika, Szpakowski, Piotr, Turniak, Malgorzata, Szemraj, Janusz, Glabinski, Andrzej·2017·Journal of Molecular Neuroscience
DOI 10.1007/s12031-017-0981-2PMC PMC5696500
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Fig. 1 The mice were used in two independent experiments. In the first, one healthy animal and immunized mice were subjected to stereotactic brain microinjection of IL-17 or PBS (phosphate-buffered saline) (n = 10 per group). In the second experiment, immunized mice were sacrificed in different stages of disease (n = 10 per group)
Fig. 1 The mice were used in two independent experiments. In the first, one healthy animal and immunized mice were subjected to stereotactic brain microinjection of IL-17 or PBS (phosphate-buffered saline) (n = 10 per group). In the second experiment, immunized mice were sacrificed in different stages of disease (n = 10 per group)CC BY · PubMed Central
Fig. 2 Scatterplot of relative expression of 84 microRNAs from Mouse Inflammatory Response and Autoimmunity microRNA PCR array, analyzed in brain homogenates of mice in preclinical phase of EAE, after stereotactic microinjections of IL-17 or PBS (control) (see the “Materials and Methods” section). Gray lines in the scatterplot indicate + 2.0 and – 2.0 fold change, which were used as cutoff values for selection of candidate miRNAs (black circles represent upregulated microRNAs, black triangles represent downregulated microRNAs)
Fig. 2 Scatterplot of relative expression of 84 microRNAs from Mouse Inflammatory Response and Autoimmunity microRNA PCR array, analyzed in brain homogenates of mice in preclinical phase of EAE, after stereotactic microinjections of IL-17 or PBS (control) (see the “Materials and Methods” section). Gray lines in the scatterplot indicate + 2.0 and – 2.0 fold change, which were used as cutoff values for selection of candidate miRNAs (black circles represent upregulated microRNAs, black triangles represent downregulated microRNAs)CC BY · PubMed Central
Fig. 3 Relative expression level of microRNAs during the course of EAE. RNA isolated from the brain of mice at various clinical stages were used. Results from all disease phases (preclinical, relapse, remission) were refered to results obtained from healthy mice (control) and presented as a fold change (2−∆∆CT)
Fig. 3 Relative expression level of microRNAs during the course of EAE. RNA isolated from the brain of mice at various clinical stages were used. Results from all disease phases (preclinical, relapse, remission) were refered to results obtained from healthy mice (control) and presented as a fold change (2−∆∆CT)CC BY · PubMed Central
Fig. 4 microRNA expression level after stereotactic brain microinjections of IL-17 in healthy mice and animals in preclinical phase of EAE. RNAs isolated from brain homogenates were used (see the “Materials and Methods” section). Results obtained for animals after intracerebral injection of IL-17 were referred to the results from animals after PBS microinjections (control), and presented as a fold change (2−∆∆CT)
Fig. 4 microRNA expression level after stereotactic brain microinjections of IL-17 in healthy mice and animals in preclinical phase of EAE. RNAs isolated from brain homogenates were used (see the “Materials and Methods” section). Results obtained for animals after intracerebral injection of IL-17 were referred to the results from animals after PBS microinjections (control), and presented as a fold change (2−∆∆CT)CC BY · PubMed Central
Fig. 5 Computational analysis of miR-155-5p target mRNAs and altered pathway—neurotrophin signaling pathway. Targets analyzed in our study are marked in gray squares (graph based on the KEGG pathway mmu04722 (Kanehisa and Goto 2000; Kanehisa et al. 2016))
Fig. 5 Computational analysis of miR-155-5p target mRNAs and altered pathway—neurotrophin signaling pathway. Targets analyzed in our study are marked in gray squares (graph based on the KEGG pathway mmu04722 (Kanehisa and Goto 2000; Kanehisa et al. 2016))CC BY · PubMed Central
Fig. 6 mRNA expression level after stereotactic brain microinjections of IL-17 to mice in preclinical phase of EAE. RNAs isolated from brain homogenates were used (see the “Materials and Methods” section). Results obtained for animals after intracerebral injection of IL-17 were referred to the results obtained for animals after PBS microinjections (control), and presented as a fold change (2−∆∆CT) ***p < 0.001
Fig. 6 mRNA expression level after stereotactic brain microinjections of IL-17 to mice in preclinical phase of EAE. RNAs isolated from brain homogenates were used (see the “Materials and Methods” section). Results obtained for animals after intracerebral injection of IL-17 were referred to the results obtained for animals after PBS microinjections (control), and presented as a fold change (2−∆∆CT) ***p < 0.001CC BY · PubMed Central
Fig. 7 Analysis of correlation between miR-155-5p and mRNA for: BDNF (a), HRAS (b), and BCL2 (c). Analysis was performed for results obtained from mice in preclinical phase of disease, after intracerebral injections of IL-17 or PBS (control). r Pearson’s correlation coefficient, R Spearman’s correlation coefficient
Fig. 7 Analysis of correlation between miR-155-5p and mRNA for: BDNF (a), HRAS (b), and BCL2 (c). Analysis was performed for results obtained from mice in preclinical phase of disease, after intracerebral injections of IL-17 or PBS (control). r Pearson’s correlation coefficient, R Spearman’s correlation coefficientCC BY · PubMed Central
Fig. 8 The protein level studied in the homogenates of brains from preclinical EAE mice after intracerebral injection of IL-17 or PBS (control)—BDNF (a); HRas (b); Bcl-2 (c) expression. Data are presented as mean ± SD; *p < 0.05
Fig. 8 The protein level studied in the homogenates of brains from preclinical EAE mice after intracerebral injection of IL-17 or PBS (control)—BDNF (a); HRas (b); Bcl-2 (c) expression. Data are presented as mean ± SD; *p < 0.05CC BY · PubMed Central

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Research works printed but not linked: 61

  1. Agarwal V, Bell GW, Nam JW, Bartel DP (2015) Predicting effective 665microRNA target sites in mammalian mRNAs. Elife. 4. doi: 10.7554/eLife.05005 · DOI · Paper page
  2. Albanesi C, Cavani A, Girolomoni G (1999) IL-17 is produced by nickel-specific T lymphocytes and regulates ICAM-1 expression and chemokine production in human keratinocytes: synergistic or antagonist effects with IFN-gamma and TNF-alpha. J Immunol 162:494–502 · DOI · Paper page
  3. Almeida RD et al (2005) Neuroprotection by BDNF against glutamate-induced apoptotic cell death is mediated by ERK and PI3-kinase pathways. Cell Death Differ 12:1329–1343. doi: 10.1038/sj.cdd.4401662 · DOI
  4. An JJ et al (2008) Distinct role of long 3′ UTR BDNF mRNA in spine morphology and synaptic plasticity in hippocampal neurons. Cell 134:175–187. doi: 10.1016/j.cell.2008.05.045 · DOI
  5. Antonsson B (2004) Mitochondria and the Bcl-2 family proteins in apoptosis signaling pathways. Mol Cell Biochem 256-257:141–155 · DOI
  6. Bartel DP (2009) MicroRNAs: target recognition and regulatory functions. Cell 136:215–233. doi: 10.1016/j.cell.2009.01.002 · DOI · Paper page
  7. Bonni A, Brunet A, West AE, Datta SR, Takasu MA, Greenberg ME (1999) Cell survival promoted by the Ras-MAPK signaling pathway by transcription-dependent and -independent mechanisms. Science 286:1358–1362 · DOI
  8. Chen CY, Faller DV (1996) Phosphorylation of Bcl-2 protein and association with p21Ras in Ras-induced apoptosis. J Biol Chem 271:2376–2379 · DOI
  9. Compston A, Coles A (2008) Multiple sclerosis. Lancet 372:1502–1517. doi: 10.1016/S0140-6736(08)61620-7 · DOI
  10. Cox AD, Der CJ (2003) The dark side of Ras: regulation of apoptosis. Oncogene 22:8999–9006. doi: 10.1038/sj.onc.1207111 · DOI
  11. Cox MB et al (2010) MicroRNAs miR-17 and miR-20a inhibit T cell activation genes and are under-expressed in MS whole blood. PLoS One 5:e12132. doi: 10.1371/journal.pone.0012132 · DOI
  12. Dai R, Ahmed SA (2011) MicroRNA, a new paradigm for understanding immunoregulation, inflammation, and autoimmune diseases. Transl Res 157:163–179. doi: 10.1016/j.trsl.2011.01.007 · DOI
  13. De Santi L, Cantalupo L, Tassi M, Raspadori D, Cioni C, Annunziata P (2009) Higher expression of BDNF receptor gp145trkB is associated with lower apoptosis intensity in T cell lines in multiple sclerosis. J Neurol Sci 277:65–70. doi: 10.1016/j.jns.2008.10.006 · DOI
  14. Denis GV, Yu Q, Ma P, Deeds L, Faller DV, Chen CY (2003) Bcl-2, via its BH4 domain, blocks apoptotic signaling mediated by mitochondrial Ras. J Biol Chem 278:5775–5785. doi: 10.1074/jbc.M210202200 · DOI
  15. Deshmukh M, Johnson EM (1997) Programmed cell death in neurons: focus on the pathway of nerve growth factor deprivation-induced death of sympathetic neurons. Mol Pharmacol 51:897–906 · DOI
  16. Dunand-Sauthier I et al (2011) Silencing of c-Fos expression by microRNA-155 is critical for dendritic cell maturation and function. Blood 117:4490–4500. doi: 10.1182/blood-2010-09-308064 · DOI
  17. Edling AE, Nanavati T, Johnson JM, Tuohy VK (2004) Human and murine lymphocyte neurotrophin expression is confined to B cells. J Neurosci Res 77:709–717. doi: 10.1002/jnr.20176 · DOI
  18. Finkbeiner S, Tavazoie SF, Maloratsky A, Jacobs KM, Harris KM, Greenberg ME (1997) CREB: a major mediator of neuronal neurotrophin responses. Neuron 19:1031–1047 · DOI
  19. Glabinski AR, Tani M, Tuohy VK, Ransohoff RM (1997) Murine experimental autoimmune encephalomyelitis: a model of immune-mediated inflammation and multiple sclerosis. Methods Enzymol 288:182–190 · DOI
  20. Hamour S et al (2015) Local IL-17 production exerts a protective role in murine experimental glomerulonephritis. PLoS One 10:e0136238. doi: 10.1371/journal.pone.0136238 · DOI
  21. Hempstead BL (2002) The many faces of p75NTR. Curr Opin Neurobiol 12:260–267 · DOI · Paper page
  22. Hu MH et al (2014) Neuroprotection effect of interleukin (IL)-17 secreted by reactive astrocytes is emerged from a high-level IL-17-containing environment during acute neuroinflammation. Clin Exp Immunol 175:268–284. doi: 10.1111/cei.12219 · DOI
  23. Jevtić B, Timotijević G, Stanisavljević S, Momčilović M, Mostarica Stojković M, Miljković D (2015) Micro RNA-155 participates in re-activation of encephalitogenic T cells. Biomed Pharmacother 74:206–210. doi: 10.1016/j.biopha.2015.08.011 · DOI
  24. Junker A (2011) Pathophysiology of translational regulation by microRNAs in multiple sclerosis. FEBS Lett 585:3738–3746. doi: 10.1016/j.febslet.2011.03.052 · DOI
  25. Junker A et al (2009) MicroRNA profiling of multiple sclerosis lesions identifies modulators of the regulatory protein CD47. Brain 132:3342–3352. doi: 10.1093/brain/awp300 · DOI · Paper page
  26. Kanehisa M, Goto S (2000) KEGG: Kyoto encyclopedia of genes and genomes. Nucleic Acids Res 28:27–30 · DOI · Paper page
  27. Kanehisa M, Sato Y, Kawashima M, Furumichi M, Tanabe M (2016) KEGG as a reference resource for gene and protein annotation. Nucleic Acids Res 44:D457–D462. doi: 10.1093/nar/gkv1070 · DOI · Paper page
  28. Ke Y, Liu K, Huang GQ, Cui Y, Kaplan HJ, Shao H, Sun D (2009) Anti-inflammatory role of IL-17 in experimental autoimmune uveitis. J Immunol 182:3183–3190. doi: 10.4049/jimmunol.0802487 · DOI
  29. Kerschensteiner M et al (1999) Activated human T cells, B cells, and monocytes produce brain-derived neurotrophic factor in vitro and in inflammatory brain lesions: a neuroprotective role of inflammation? J Exp Med 189:865–870 · DOI
  30. Kruse N, Cetin S, Chan A, Gold R, Lühder F (2007) Differential expression of BDNF mRNA splice variants in mouse brain and immune cells. J Neuroimmunol 182:13–21. doi: 10.1016/j.jneuroim.2006.09.001 · DOI
  31. Ksiazek-Winiarek DJ, Kacperska MJ, Glabinski A (2013, 2013) MicroRNAs as novel regulators of neuroinflammation. Mediators Inflamm:172351. doi: 10.1155/2013/172351 · DOI
  32. Linker RA et al (2010) Functional role of brain-derived neurotrophic factor in neuroprotective autoimmunity: therapeutic implications in a model of multiple sclerosis. Brain 133:2248–2263. doi: 10.1093/brain/awq179 · DOI
  33. Lisak RP, Benjamins JA, Bealmear B, Yao B, Land S, Nedelkoska L, Skundric D (2006) Differential effects of Th1, monocyte/macrophage and Th2 cytokine mixtures on early gene expression for immune-related molecules by central nervous system mixed glial cell cultures. Mult Scler 12:149–168. doi: 10.1191/135248506ms1251oa · DOI
  34. Lisak RP, Benjamins JA, Bealmear B, Nedelkoska L, Yao B, Land S, Studzinski D (2007) Differential effects of Th1, monocyte/macrophage and Th2 cytokine mixtures on early gene expression for glial and neural-related molecules in central nervous system mixed glial cell cultures: neurotrophins, growth factors and structural proteins. J Neuroinflammation 4:30. doi: 10.1186/1742-2094-4-30 · DOI
  35. Littman DR, Rudensky AY (2010) Th17 and regulatory T cells in mediating and restraining inflammation. Cell 140:845–858. doi: 10.1016/j.cell.2010.02.021 · DOI · Paper page
  36. Liu QR, Lu L, Zhu XG, Gong JP, Shaham Y, Uhl GR (2006) Rodent BDNF genes, novel promoters, novel splice variants, and regulation by cocaine. Brain Res 1067:1–12. doi: 10.1016/j.brainres.2005.10.004 · DOI
  37. Lock C et al (2002) Gene-microarray analysis of multiple sclerosis lesions yields new targets validated in autoimmune encephalomyelitis. Nat Med 8:500–508. doi: 10.1038/nm0502-500 · DOI
  38. Lopez-Ramirez MA et al (2014) MicroRNA-155 negatively affects blood-brain barrier function during neuroinflammation. FASEB J 28:2551–2565. doi: 10.1096/fj.13-248880 · DOI · Paper page
  39. Ma X et al (2014) Expression, regulation and function of microRNAs in multiple sclerosis. Int J Med Sci 11:810–818. doi: 10.7150/ijms.8647 · DOI
  40. Makar TK, Trisler D, Sura KT, Sultana S, Patel N, Bever CT (2008) Brain derived neurotrophic factor treatment reduces inflammation and apoptosis in experimental allergic encephalomyelitis. J Neurol Sci 270:70–76. doi: 10.1016/j.jns.2008.02.011 · DOI
  41. Matusevicius D, Kivisäkk P, He B, Kostulas N, Ozenci V, Fredrikson S, Link H (1999) Interleukin-17 mRNA expression in blood and CSF mononuclear cells is augmented in multiple sclerosis. Mult Scler 5:101–104 · DOI
  42. Michaelidis TM, Sendtner M, Cooper JD, Airaksinen MS, Holtmann B, Meyer M, Thoenen H (1996) Inactivation of bcl-2 results in progressive degeneration of motoneurons, sympathetic and sensory neurons during early postnatal development. Neuron 17:75–89 · DOI
  43. Ng HP, Burris RL, Nagarajan S (2011) Attenuated atherosclerotic lesions in apoE-Fcγ-chain-deficient hyperlipidemic mouse model is associated with inhibition of Th17 cells and promotion of regulatory T cells. J Immunol 187:6082–6093. doi: 10.4049/jimmunol.1004133 · DOI
  44. O’Connell RM, Taganov KD, Boldin MP, Cheng G, Baltimore D (2007) MicroRNA-155 is induced during the macrophage inflammatory response. Proc Natl Acad Sci U S A 104:1604–1609. doi: 10.1073/pnas.0610731104 · DOI · Paper page
  45. O’Connell RM et al (2010a) MicroRNA-155 promotes autoimmune inflammation by enhancing inflammatory T cell development. Immunity 33:607–619. doi: 10.1016/j.immuni.2010.09.009 · DOI · Paper page
  46. O’Connell RM, Rao DS, Chaudhuri AA, Baltimore D (2010b) Physiological and pathological roles for microRNAs in the immune system. Nat Rev Immunol 10:111–122. doi: 10.1038/nri2708 · DOI · Paper page
  47. Popescu BF, Lucchinetti CF (2012) Pathology of demyelinating diseases. Annu Rev Pathol 7:185–217. doi: 10.1146/annurev-pathol-011811-132443 · DOI
  48. Spriggs MK (1997) Interleukin-17 and its receptor. J Clin Immunol 17:366–369 · DOI
  49. Stacey DW, Watson T, Kung HF, Curran T (1987) Microinjection of transforming ras protein induces c-fos expression. Mol Cell Biol 7:523–527 · DOI
  50. Stadelmann C, Kerschensteiner M, Misgeld T, Brück W, Hohlfeld R, Lassmann H (2002) BDNF and gp145trkB in multiple sclerosis brain lesions: neuroprotective interactions between immune and neuronal cells? Brain 125:75–85 · DOI
  51. Stromnes IM, Cerretti LM, Liggitt D, Harris RA, Goverman JM (2008) Differential regulation of central nervous system autoimmunity by T(H)1 and T(H)17 cells. Nat Med 14:337–342. doi: 10.1038/nm1715 · DOI
  52. Thoenen H (1995) Neurotrophins and neuronal plasticity. Science 270:593–598 · DOI · Paper page
  53. Trapp BD, Ransohoff R, Rudick R (1999) Axonal pathology in multiple sclerosis: relationship to neurologic disability. Curr Opin Neurol 12:295–302 · DOI
  54. Vanhoutte P, Barnier JV, Guibert B, Pagès C, Besson MJ, Hipskind RA, Caboche J (1999) Glutamate induces phosphorylation of Elk-1 and CREB, along with c-fos activation, via an extracellular signal-regulated kinase-dependent pathway in brain slices. Mol Cell Biol 19:136–146 · DOI
  55. Varendi K, Kumar A, Härma MA, Andressoo JO (2014) miR-1, miR-10b, miR-155, and miR-191 are novel regulators of BDNF. Cell Mol Life Sci 71:4443–4456. doi: 10.1007/s00018-014-1628-x · DOI · Paper page
  56. Vlachos IS et al (2015) DIANA-miRPath v3.0: deciphering microRNA function with experimental support. Nucleic Acids Res 43:W460–W466. doi: 10.1093/nar/gkv403 · DOI
  57. Witowski J et al (2000) IL-17 stimulates intraperitoneal neutrophil infiltration through the release of GRO alpha chemokine from mesothelial cells. J Immunol 165:5814–5821 · DOI
  58. Wojkowska DW, Szpakowski P, Ksiazek-Winiarek D, Leszczynski M, Glabinski A (2014) Interactions between neutrophils, Th17 cells, and chemokines during the initiation of experimental model of multiple sclerosis. Mediat Inflamm 2014:590409. doi: 10.1155/2014/590409 · DOI
  59. Wong LF et al (2005) Lentiviral-mediated delivery of Bcl-2 or GDNF protects against excitotoxicity in the rat hippocampus. Mol Ther 11:89–95. doi: 10.1016/j.ymthe.2004.08.026 · DOI
  60. Xiao F, Zuo Z, Cai G, Kang S, Gao X, Li T (2009) miRecords: an integrated resource for microRNA-target interactions. Nucleic Acids Res 37:D105–D110. doi: 10.1093/nar/gkn851 · DOI
  61. Xue L, Murray JH, Tolkovsky AM (2000) The Ras/phosphatidylinositol 3-kinase and Ras/ERK pathways function as independent survival modules each of which inhibits a distinct apoptotic signaling pathway in sympathetic neurons. J Biol Chem 275:8817–8824 · DOI

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Metadata

Authors
Ksiazek-Winiarek, D., Szpakowski, P., Turniak, M., Szemraj, J., & Glabinski, A.
Venue
Journal of Molecular Neuroscience
Publisher
Springer Science and Business Media LLC
Published
2017-10-23
Volume
63
Issue
3-4
Pages
320-332
ISSN
  • 0895-8696
  • 1559-1166
DOI
10.1007/s12031-017-0981-2
PMC
PMC5696500
Cited by
26
References
4
Language
en
Licence
creativecommons.org/licenses/by/4.0 (unspecified)
Funders
  • Narodowe Centrum Nauki (DEC-2012/05/N/NZ4/02347)
  • Uniwersytet Medyczny w Lodzi (503/5-062-01/503-51-002)

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Ksiazek-Winiarek, D., Szpakowski, P., Turniak, M., Szemraj, J., & Glabinski, A. (2017). IL-17 Exerts Anti-Apoptotic Effect via miR-155-5p Downregulation in Experimental Autoimmune Encephalomyelitis. Journal of Molecular Neuroscience. https://doi.org/10.1007/s12031-017-0981-2