Cited by 172Electrochemical sensors and biosensorsPolymer Surface Interaction StudiesNeuroscience and Neural Engineering

Photo-crosslinked copolymers of 2-hydroxyethyl methacrylate, poly(ethylene glycol) tetra-acrylate and ethylene dimethacrylate for improving biocompatibility of biosensors

Open access
Quinn, Chris P., Pathak, Chandrashekhar P., Heller, Adam, Hubbell, Jeffrey A.·1995·Biomaterials

Abstract

A copolymer containing 88% 2-hydroxyethyl methacrylate (HEMA), 9% poly(ethylene glycol) (MW 18.5 kDa) tetra-acrylate and 3% ethylene dimethacrylate was prepared and evaluated for use as a biocompatible interface between glucose biosensors and tissue in the rat. The glucose sensor utilizes glucose oxidase that is electrically 'wired' to a gold current collector by a reduction-oxidation polymer. Coatings of the copolymer were crosslinked in situ on the sensors using long wavelength ultraviolet light and 2,2-dimethoxy-2-phenyl-acetophenone as the initiator. The effect these films had on the current response to glucose was measured. Over a glucose concentration range of 0-30 mM, the average percentage decrease in response was 45 +/- 28% (mean +/- 95% confidence interval) at 37 degrees C for films that were about 0.1 mm thick, an acceptable value. Copolymer-treated and control electrodes were implanted in the intrascapular subcutaneous tissue of male Sprague-Dawley rats for three days. The explanted samples were evaluated using scanning electron microscopy. The control electrodes were highly encapsulated with fibrous material, while the copolymer-treated electrodes induced much less encapsulation. The results show this copolymer to be a candidate as a biocompatible coating for electrically wired oxidoreductase-based subcutaneous biosensors.

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Crossref · 10.1016/0142-9612(95)98856-9

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  • Substrates with a hydrogel coating had markedly less fibrous encapsulation than controls after 3 days.

    “substrates with the hydrogel coating had markedly less fibrous encapsulation relative to controls.143”

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Authors
Quinn, C. P., Pathak, C. P., Heller, A., & Hubbell, J. A.
Affiliations
The University of Texas at Austin (US)
Corresponding author
Hubbell, Jeffrey A.
Venue
Biomaterials
Publisher
Elsevier BV
Published
1995-01
Volume
16
Issue
5
Pages
389-396
ISSN
0142-9612 (ISSN-L)
OpenAlex
W1988024774
Cited by
172
References
19
Citations by year
2025: 5 · 2023: 2 · 2022: 2 · 2021: 3 · 2020: 2 · 2019: 4 · 2018: 4 · 2017: 4 · 2016: 5 · 2015: 4 · 2014: 8 · 2013: 8 · 2012: 7
FWCI
5.74
Type
journal article
Language
en
Open access
Locations
  • Biomaterials (publishedVersion)
  • PubMed (publishedVersion)
  • Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion)
  • Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
Funders
  • National Institutes of Health (5r01dk042015-07)
  • National Institute of Diabetes and Digestive and Kidney Diseases (DK42015)
Topics
  • Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
  • Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neural Engineering
Keywords
2-hydroxyethyl methacrylate, ethylene glycol dimethacrylate, glucose biosensor, biocompatibility, subcutaneous implantation, glucose oxidase, gold electrode, fibrous encapsulation, in situ crosslinking, 2,2-dimethoxy-2-phenylacetophenone
MeSH terms
Acrylates, Animals, Biocompatible Materials, Calibration, Cross-Linking Reagents, Glucose, Male, Methacrylates, Photochemistry, Polyethylene Glycols, Polymers, Ultraviolet Rays, Biosensing Techniques, Rats, Sprague-Dawley, Rats

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