Approaches to surface engineering of extracellular vesicles

Shedding light on the cell biology of extracellular vesicles

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Biological properties of extracellular vesicles and their physiological functions

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Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes

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PEGylation as a strategy for improving nanoparticle-based drug and gene delivery

Suk, J. S., Xu, Q., Kim, N., Hanes, J., & Ensign, L. M. (2016) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2015.09.012 · Cited by 4,523 · see citing works

Specificities of secretion and uptake of exosomes and other extracellular vesicles for cell-to-cell communication

Mathieu, M., Jaular, L. M., Lavieu, G., & Théry, C. (2018) · Nature Cell Biology · doi:10.1038/s41556-018-0250-9 · Cited by 3,776 · see citing works

Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET

Peinado, H., Alečković, M., Lavotshkin, S., Matei, I., Costa-Silva, B., Moreno-Bueno, G., Hergueta-Redondo, M., Williams, C., García-Santos, G., Ghajar, C. M., et al. (2012) · Nature Medicine · doi:10.1038/nm.2753 · Cited by 3,715 · see citing works

Current knowledge on exosome biogenesis and release

Hessvik, N. P., & Llorente, A. (2018) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-017-2595-9 · Cited by 2,362 · see citing works

Exosomes as drug delivery vehicles for Parkinson’s disease therapy

Haney, M. J., Klyachko, N. L., Zhao, Y., Gupta, R., Plotnikova, E. G., He, Z., Patel, T., Piroyan, A., Sokolsky, M., Kabanov, A. V., et al. (2015) · Journal of Controlled Release · doi:10.1016/j.jconrel.2015.03.033 · Cited by 2,009 · see citing works

Progress in Exosome Isolation Techniques

Li, P., Kaslan, M., Lee, S. H., Yao, J. D., & Gao, Z. (2017) · Theranostics · doi:10.7150/thno.18133 · Cited by 1,970 · see citing works

Outer-membrane vesicles from Gram-negative bacteria: biogenesis and functions

Schwechheimer, C., & Kuehn, M. J. (2015) · Nature Reviews Microbiology · doi:10.1038/nrmicro3525 · Cited by 1,932 · see citing works

Extracellular vesicle in vivo biodistribution is determined by cell source, route of administration and targeting

Wiklander, O. P. B., Nordin, J. Z., O'Loughlin, A., Gustafsson, Y., Corso, G., Mäger, I., Vader, P., Lee, Y., Sork, H., Seow, Y., et al. (2015) · Journal of Extracellular Vesicles · doi:10.3402/jev.v4.26316 · Cited by 1,730 · see citing works

Optimized exosome isolation protocol for cell culture supernatant and human plasma

Lobb, R. J., Becker, M., Wen, S., Wong, C. S. F., Wiegmans, A. P., Leimgruber, A., & Möller, A. (2015) · Journal of Extracellular Vesicles · doi:10.3402/jev.v4.27031 · Cited by 1,725 · see citing works

Systemically Injected Exosomes Targeted to EGFR Deliver Antitumor MicroRNA to Breast Cancer Cells

Ohno, S.-I., Takanashi, M., Sudo, K., Ueda, S., Ishikawa, A., Matsuyama, N., Fujita, K., Mizutani, T., Ohgi, T., Ochiya, T., et al. (2013) · Molecular Therapy · doi:10.1038/mt.2012.180 · Cited by 1,666 · see citing works

Biological Functions and Biogenesis of Secreted Bacterial Outer Membrane Vesicles

Kulp, A., & Kuehn, M. J. (2010) · Annual Review of Microbiology · doi:10.1146/annurev.micro.091208.073413 · Cited by 1,540 · see citing works

Through the wall: extracellular vesicles in Gram-positive bacteria, mycobacteria and fungi

Brown, L. C., Wolf, J. M., Prados‐Rosales, R., & Casadevall, A. (2015) · Nature Reviews Microbiology · doi:10.1038/nrmicro3480 · Cited by 1,335 · see citing works

Single‐step isolation of extracellular vesicles by size‐exclusion chromatography

Böing, A. N., van der Pol, E., Grootemaat, A. E., Coumans, F. A. W., Sturk, A., & Nieuwland, R. (2014) · Journal of Extracellular Vesicles · doi:10.3402/jev.v3.23430 · Cited by 1,228 · see citing works

Extracellular vesicles as drug delivery systems: Why and how?

Elsharkasy, O. M., Nordin, J. Z., Hagey, D. W., de Jong, O. G., Schiffelers, R. M., Andaloussi, S. E., & Vader, P. (2020) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2020.04.004 · Cited by 1,217 · see citing works

Surface functionalized exosomes as targeted drug delivery vehicles for cerebral ischemia therapy

Tian, T., Zhang, H.-X., He, C.-P., Fan, S., Zhu, Y.-L., Qi, C., Huang, N.-P., Xiao, Z.-D., Lu, Z.-H., Tannous, B. A., et al. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2017.10.012 · Cited by 1,154 · see citing works

Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey

Gardiner, C., Vizio, D. D., Sahoo, S., Théry, C., Witwer, K. W., Wauben, M. H. M., & Hill, A. F. (2016) · Journal of Extracellular Vesicles · doi:10.3402/jev.v5.32945 · Cited by 1,075 · see citing works

Advances in therapeutic applications of extracellular vesicles

Wiklander, O. P. B., Brennan, M. Á., Lötvall, J., Breakefield, X. O., & EL Andaloussi, S. (2019) · Science Translational Medicine · doi:10.1126/scitranslmed.aav8521 · Cited by 1,039 · see citing works

Mechanism of hard-nanomaterial clearance by the liver

Tsoi, K. M., MacParland, S. A., Ma, X., Spetzler, V. N., Echeverri, J. A., Ouyang, B., Fadel, S. M., Sykes, E. A., Goldaracena, N., Kaths, J. M., et al. (2016) · Nature Materials · doi:10.1038/nmat4718 · Cited by 1,020 · see citing works

Minimal "Self" Peptides That Inhibit Phagocytic Clearance and Enhance Delivery of Nanoparticles

Rodriguez, P. L., Harada, T., Christian, D. A., Pantano, D. A., Tsai, R. K., & Discher, D. E. (2013) · Science · doi:10.1126/science.1229568 · Cited by 985 · see citing works

The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling

Deun, J. V., Mestdagh, P., Sormunen, R. T., Cocquyt, V., Vermaelen, K., Vandesompele, J., Bracke, M., Wever, O. D., & Hendrix, A. (2014) · Journal of Extracellular Vesicles · doi:10.3402/jev.v3.24858 · Cited by 941 · see citing works

A Review of Exosomal Isolation Methods: Is Size Exclusion Chromatography the Best Option?

Sidhom, K., Obi, P. O., & Saleem, A. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21186466 · Cited by 908 · see citing works

Dynamic Biodistribution of Extracellular Vesicles in Vivo Using a Multimodal Imaging Reporter

Lai, C. P., Mardini, O., Ericsson, M., Prabhakar, S., Maguire, C. A., Chen, J. W., Tannous, B. A., & Breakefield, X. O. (2014) · ACS Nano · doi:10.1021/nn404945r · Cited by 894 · see citing works

Membrane vesicles traffic signals and facilitate group activities in a prokaryote

Mashburn, L. M., & Whiteley, M. (2005) · Nature · doi:10.1038/nature03925 · Cited by 844 · see citing works

Membrane Vesicle Release in Bacteria, Eukaryotes, and Archaea: a Conserved yet Underappreciated Aspect of Microbial Life

Deatherage, B. L., & Cookson, B. T. (2012) · Infection and Immunity · doi:10.1128/iai.06014-11 · Cited by 765 · see citing works

Engineering hybrid exosomes by membrane fusion with liposomes

Sato, Y. T., Umezaki, K., Sawada, S., Mukai, S.-A., Sasaki, Y., Harada, N., Shiku, H., & Akiyoshi, K. (2016) · Scientific Reports · doi:10.1038/srep21933 · Cited by 744 · see citing works

Visualization and in vivo tracking of the exosomes of murine melanoma B16-BL6 cells in mice after intravenous injection

Takahashi, Y., Nishikawa, M., Shinotsuka, H., Matsui, Y., Ohara, S., Imai, T., & Takakura, Y. (2013) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2013.03.013 · Cited by 729 · see citing works

Biodistribution and delivery efficiency of unmodified tumor-derived exosomes

Smyth, T., Kullberg, M., Malik, N., Smith-Jones, P. M., Graner, M. W., & Anchordoquy, T. J. (2014) · Journal of Controlled Release · doi:10.1016/j.jconrel.2014.12.013 · Cited by 727 · see citing works

Electroporation-induced siRNA precipitation obscures the efficiency of siRNA loading into extracellular vesicles

Kooijmans, S. A. A., Stremersch, S., Braeckmans, K., de Smedt, S. C., Hendrix, A., Wood, M. J. A., Schiffelers, R. M., Raemdonck, K., & Vader, P. (2013) · Journal of Controlled Release · doi:10.1016/j.jconrel.2013.08.014 · Cited by 663 · see citing works

Copper-free click chemistry in living animals

Chang, P. V., Prescher, J. A., Sletten, E. M., Baskin, J. M., Miller, I. A., Agard, N. J., Lo, A., & Bertozzi, C. R. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0911116107 · Cited by 656 · see citing works

PEGylated and targeted extracellular vesicles display enhanced cell specificity and circulation time

Kooijmans, S. A. A., Fliervoet, L. A. L., van der Meel, R., Fens, M. H. A. M., Heijnen, H. F. G., Henegouwen, P. M. P. V. B. E., Vader, P., & Schiffelers, R. M. (2016) · Journal of Controlled Release · doi:10.1016/j.jconrel.2016.01.009 · Cited by 629 · see citing works

Bacterial outer membrane vesicles suppress tumor by interferon-γ-mediated antitumor response

Kim, O. Y., Park, H. T., Dinh, N. T. H., Choi, S. J., Lee, J., Kim, J. H., Lee, S.-W., & Gho, Y. S. (2017) · Nature Communications · doi:10.1038/s41467-017-00729-8 · Cited by 616 · see citing works

High‐speed centrifugation induces aggregation of extracellular vesicles

Linares, R., Tan, S., Gounou, C., Arraud, N., & Brisson, A. R. (2015) · Journal of Extracellular Vesicles · doi:10.3402/jev.v4.29509 · Cited by 572 · see citing works

Human adipose tissue-derived mesenchymal stem cells secrete functional neprilysin-bound exosomes

Katsuda, T., Tsuchiya, R., Kosaka, N., Yoshioka, Y., Takagaki, K., Oki, K., Takeshita, F., Sakai, Y., Kuroda, M., & Ochiya, T. (2013) · Scientific Reports · doi:10.1038/srep01197 · Cited by 565 · see citing works

Bioorthogonal Reactions for Labeling Proteins

Lang, K., & Chin, J. W. (2014) · ACS Chemical Biology · doi:10.1021/cb4009292 · Cited by 556 · see citing works

Surface Functionalization of Exosomes Using Click Chemistry

Smyth, T., Petrova, K., Payton, N. M., Persaud, I., Redzic, J. S., Graner, M. W., Smith-Jones, P., & Anchordoquy, T. J. (2014) · Bioconjugate Chemistry · doi:10.1021/bc500291r · Cited by 532 · see citing works

A novel affinity-based method for the isolation of highly purified extracellular vesicles

Nakai, W., Yoshida, T., Díez, D., Miyatake, Y., Nishibu, T., Imawaka, N., Naruse, K., Sadamura, Y., & Hanayama, R. (2016) · Scientific Reports · doi:10.1038/srep33935 · Cited by 486 · see citing works

Exosome-mediated delivery of hydrophobically modified siRNA for huntingtin mRNA silencing

Didiot, M.-C., Hall, L. M., Coles, A. H., Haraszti, R. A., Godinho, B. M., Chase, K., Sapp, E., Ly, S., Alterman, J. F., Hassler, M. R., et al. (2016) · Molecular Therapy · doi:10.1038/mt.2016.126 · Cited by 483 · see citing works

Modification of Extracellular Vesicles by Fusion with Liposomes for the Design of Personalized Biogenic Drug Delivery Systems

Piffoux, M., Silva, A., Wilhelm, C., Gazeau, F., & Tareste, D. C. (2018) · ACS Nano · doi:10.1021/acsnano.8b02053 · Cited by 463 · see citing works

Display of GPI‐anchored anti‐EGFR nanobodies on extracellular vesicles promotes tumour cell targeting

Kooijmans, S. A. A., Gomez‐Aleza, C., Roffler, S. R., van Solinge, W. W., Vader, P., & Schiffelers, R. M. (2016) · Journal of Extracellular Vesicles · doi:10.3402/jev.v5.31053 · Cited by 429 · see citing works

Acute Inflammatory Response to Endotoxin in Mice and Humans

Copeland, S., Warren, H. S., Lowry, S. F., Calvano, S. E., & Remick, D. G. (2005) · Clinical and Vaccine Immunology · doi:10.1128/cdli.12.1.60-67.2005 · Cited by 427 · see citing works

Anchor peptide captures, targets, and loads exosomes of diverse origins for diagnostics and therapy

Gao, X., Ran, N., Dong, X., Zuo, B., Yang, R., Zhou, Q., Moulton, H. M., Seow, Y., & Yin, H. (2018) · Science Translational Medicine · doi:10.1126/scitranslmed.aat0195 · Cited by 426 · see citing works

Outer membrane vesicles as platform vaccine technology

van der Pol, L., Stork, M., & van der Ley, P. (2015) · Biotechnology Journal · doi:10.1002/biot.201400395 · Cited by 386 · see citing works

Interleukin 3- receptor targeted exosomes inhibit in vitro and in vivo Chronic Myelogenous Leukemia cell growth

Bellavia, D., Raimondo, S., Calabrese, G., Forte, S., Cristaldi, M., Patinella, A., Memeo, L., Manno, M., Raccosta, S., Diana, P., et al. (2017) · Theranostics · doi:10.7150/thno.17092 · Cited by 383 · see citing works

Antigen Presentation by Extracellular Vesicles from Professional Antigen-Presenting Cells

Lindenbergh, M. F. S., & Stoorvogel, W. (2018) · Annual Review of Immunology · doi:10.1146/annurev-immunol-041015-055700 · Cited by 376 · see citing works

Stabilization of Exosome-targeting Peptides via Engineered Glycosylation

Hung, M. E., & Leonard, J. N. (2015) · Journal of Biological Chemistry · doi:10.1074/jbc.m114.621383 · Cited by 368 · see citing works

PKH26 labeling of extracellular vesicles: Characterization and cellular internalization of contaminating PKH26 nanoparticles

Dominkuš, P. P., Stenovec, M., Sitar, S., Lasič, E., Zorec, R., Plemenitaš, A., Žagar, E., Kreft, M., & Lenassi, M. (2018) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2018.03.013 · Cited by 358 · see citing works

Extracellular Vesicles—Connecting Kingdoms

Woith, E., Fuhrmann, G., & Melzig, M. F. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20225695 · Cited by 331 · see citing works

Insertion of poly(ethylene glycol) derivatized phospholipid into pre‐formed liposomes results in prolonged in vivo circulation time

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Targeting Tumor Antigens to Secreted Membrane Vesicles In vivo Induces Efficient Antitumor Immune Responses

Zeelenberg, I. S., Ostrowski, M., Krumeich, S., Bobrie, A., Jancic, C. C., Boissonnas, A., Delcayre, A. X., Pecq, J. L., Combadière, B., Amigorena, S., et al. (2008) · Cancer Research · doi:10.1158/0008-5472.can-07-3163 · Cited by 286 · see citing works

Reprogramming Exosomes as Nanoscale Controllers of Cellular Immunity

Cheng, Q., Shi, X., Han, M., Smbatyan, G., Lenz, H., & Zhang, Y. (2018) · Journal of the American Chemical Society · doi:10.1021/jacs.8b10047 · Cited by 286 · see citing works

Quantitative Analysis of Tissue Distribution of the B16BL6-Derived Exosomes Using a Streptavidin-Lactadherin Fusion Protein and Iodine-125-Labeled Biotin Derivative After Intravenous Injection in Mice

Morishita, M., Takahashi, Y., Nishikawa, M., Sano, K., Kato, K., Yamashita, T., Imai, T., Saji, H., & Takakura, Y. (2014) · Journal of Pharmaceutical Sciences · doi:10.1002/jps.24251 · Cited by 285 · see citing works

Extracellular vesicles in liver disease and potential as biomarkers and therapeutic targets

Szabó, G., & Momen‐Heravi, F. (2017) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/nrgastro.2017.71 · Cited by 283 · see citing works

Aptamer-Conjugated Extracellular Nanovesicles for Targeted Drug Delivery

Wan, Y., Wang, L., Zhu, C., Zheng, Q., Wang, G., Tong, J., Fang, Y., Xia, Y., Cheng, G., He, X., et al. (2017) · Cancer Research · doi:10.1158/0008-5472.can-17-2880 · Cited by 260 · see citing works

Membrane Vesicle Formation as a Multiple-Stress Response Mechanism Enhances Pseudomonas putida DOT-T1E Cell Surface Hydrophobicity and Biofilm Formation

Baumgarten, T., Sperling, S., Seifert, J., von Bergen�, M., Steiniger, F., Wick, L. Y., & Heipieper, H. J. (2012) · Applied and Environmental Microbiology · doi:10.1128/aem.01525-12 · Cited by 250 · see citing works

Isolation of High-Purity Extracellular Vesicles by the Combination of Iodixanol Density Gradient Ultracentrifugation and Bind-Elute Chromatography From Blood Plasma

Onódi, Z., Pelyhe, C., Nagy, C. T., Brenner, G. B., Almási, L., Kittel, Á., Manček‐Keber, M., Ferdinandy, P., Buzás, E. I., & Giricz, Z. (2018) · Frontiers in Physiology · doi:10.3389/fphys.2018.01479 · Cited by 249 · see citing works

Exosomes Engineered to Express a Cardiomyocyte Binding Peptide Demonstrate Improved Cardiac Retention in Vivo

Mentkowski, K. I., & Lang, J. K. (2019) · Scientific Reports · doi:10.1038/s41598-019-46407-1 · Cited by 244 · see citing works

Regulation of Postsynaptic Retrograde Signaling by Presynaptic Exosome Release

Korkut, C., Li, Y., Koles, K., Brewer, C., Ashley, J., Yoshihara, M., & Budnik, V. (2013) · Neuron · doi:10.1016/j.neuron.2013.01.013 · Cited by 242 · see citing works

Confounding factors in vesicle uptake studies using fluorescent lipophilic membrane dyes

Takov, K., Yellon, D. M., & Davidson, S. M. (2017) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2017.1388731 · Cited by 242 · see citing works

Intestinal Anti-inflammatory Effects of Outer Membrane Vesicles from Escherichia coli Nissle 1917 in DSS-Experimental Colitis in Mice

Fábrega, M.-J., Rodríguez-Nogales, A., Garrido-Mesa, J., Algieri, F., Badía, J., Giménez, R., Gálvez, J., & Baldomà, L. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.01274 · Cited by 240 · see citing works

Focus on Extracellular Vesicles: Exosomes and Their Role in Protein Trafficking and Biomarker Potential in Alzheimer’s and Parkinson’s Disease

Vella, L. J., Hill, A. F., & Cheng, L. (2016) · International Journal of Molecular Sciences · doi:10.3390/ijms17020173 · Cited by 231 · see citing works

New insights into extracellular vesicle biogenesis and function

Latifkar, A., Hur, Y. H., Sanchez, J. C., Cerione, R. A., & Antonyak, M. A. (2019) · Journal of Cell Science · doi:10.1242/jcs.222406 · Cited by 220 · see citing works

Modification of Lipid A Biosynthesis in Neisseria meningitidis lpxL Mutants: Influence on Lipopolysaccharide Structure, Toxicity, and Adjuvant Activity

van der Ley, P., Steeghs, L., Hamstra, H.-J., ten Hove, J., Zomer, B., & van Alphen, L. (2001) · Infection and Immunity · doi:10.1128/iai.69.10.5981-5990.2001 · Cited by 215 · see citing works

Aptamer-Functionalized Exosomes: Elucidating the Cellular Uptake Mechanism and the Potential for Cancer-Targeted Chemotherapy

Zou, J., Shi, M., Liu, X., Jin, C. Y., Xing, X., Qiu, L., & Tan, W. (2019) · Analytical Chemistry · doi:10.1021/acs.analchem.8b05204 · Cited by 214 · see citing works

The application of exosomes as a nanoscale cancer vaccine

Seifalian, A. M. (2010) · International Journal of Nanomedicine · doi:10.2147/ijn.s13402 · Cited by 201 · see citing works

Development of exosome surface display technology in living human cells

Stickney, Z., Losacco, J., McDevitt, S., Zhang, Z., & Lü, B. (2016) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2016.02.058 · Cited by 200 · see citing works

Functional Delivery of Lipid-Conjugated siRNA by Extracellular Vesicles

O’Loughlin, A. J., Mäger, I., de Jong, O. G., Varela, M. A., Schiffelers, R. M., Andaloussi, S. E., Wood, M. J. A., & Vader, P. (2017) · Molecular Therapy · doi:10.1016/j.ymthe.2017.03.021 · Cited by 200 · see citing works

TRAIL delivery by MSC‐derived extracellular vesicles is an effective anticancer therapy

Yuan, Z., Kolluri, K. K., Gowers, K. H. C., & Janes, S. M. (2017) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2017.1265291 · Cited by 197 · see citing works

Increasing vaccine potency through exosome antigen targeting

Hartman, Z. C., Wei, J., Glass, O. K., Guo, H., Lei, G., Yang, X.-Y., Osada, T., Hobeika, A., Delcayre, A., Le Pecq, J.-B., et al. (2011) · Vaccine · doi:10.1016/j.vaccine.2011.09.133 · Cited by 196 · see citing works

Augmented liver targeting of exosomes by surface modification with cationized pullulan

Tamura, R., Üemoto, S., & Tabata, Y. (2017) · Acta Biomaterialia · doi:10.1016/j.actbio.2017.05.013 · Cited by 189 · see citing works

Therapeutic and diagnostic applications of extracellular vesicles

Stremersch, S., Smedt, S. C. D., & Raemdonck, K. (2016) · Journal of Controlled Release · doi:10.1016/j.jconrel.2016.07.054 · Cited by 187 · see citing works

Systematic characterization of extracellular vesicle sorting domains and quantification at the single molecule – single vesicle level by fluorescence correlation spectroscopy and single particle imaging

Corso, G., Heusermann, W., Trojer, D., Görgens, A., Steib, E., Voshol, J., Graff, A., Genoud, C., Lee, Y., Hean, J., et al. (2019) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2019.1663043 · Cited by 172 · see citing works

Integrating Protein Engineering and Bioorthogonal Click Conjugation for Extracellular Vesicle Modulation and Intracellular Delivery

Wang, M., Altınoglu, S., Takeda, Y. S., & Xu, Q. (2015) · PLoS ONE · doi:10.1371/journal.pone.0141860 · Cited by 164 · see citing works

Recombinant phosphatidylserine-binding nanobodies for targeting of extracellular vesicles to tumor cells: a plug-and-play approach

Kooijmans, S. A. A., Gitz-Francois, J. J., Schiffelers, R. M., & Vader, P. (2018) · Nanoscale · doi:10.1039/c7nr06966a · Cited by 163 · see citing works

The roles of extracellular vesicles in cancer biology: Toward the development of novel cancer biomarkers

Katsuda, T., Kosaka, N., & Ochiya, T. (2013) · PROTEOMICS · doi:10.1002/pmic.201300389 · Cited by 161 · see citing works

Clinical potential of mesenchymal stem/stromal cell-derived extracellular vesicles

Giebel, B., Kordelas, L., & Börger, V. (2017) · Stem Cell Investigation · doi:10.21037/sci.2017.09.06 · Cited by 157 · see citing works

Pitfalls associated with lipophilic fluorophore staining of extracellular vesicles for uptake studies

Simonsen, J. B. (2019) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2019.1582237 · Cited by 150 · see citing works

Comparing exosome-like vesicles with liposomes for the functional cellular delivery of small RNAs

Stremersch, S., Vandenbroucke, R. E., Wonterghem, E. V., Hendrix, A., Smedt, S. C. D., & Raemdonck, K. (2016) · Journal of Controlled Release · doi:10.1016/j.jconrel.2016.04.005 · Cited by 146 · see citing works

Exosomes Mediate Epithelium–Mesenchyme Crosstalk in Organ Development

Jiang, N., Xiang, L., He, L., Yang, G., Zheng, J., Wang, C., Zhang, Y., Wang, S., Zhou, Y., Sheu, T.-J., et al. (2017) · ACS Nano · doi:10.1021/acsnano.7b01087 · Cited by 145 · see citing works

Recombinant M2e outer membrane vesicle vaccines protect against lethal influenza A challenge in BALB/c mice

Rappazzo, C. G., Watkins, H. C., Guarino, C., Chau, A., Lopez, J. L., DeLisa, M. P., Leifer, C. A., Whittaker, G. R., & Putnam, D. A. (2016) · Vaccine · doi:10.1016/j.vaccine.2016.01.028 · Cited by 141 · see citing works

Membrane Radiolabelling of Exosomes for Comparative Biodistribution Analysis in Immunocompetent and Immunodeficient Mice - A Novel and Universal Approach

Faruqu, F. N., Wang, J. T.-W., Xu, L., McNickle, L., Chong, E. M.-Y., Walters, A. A., Gurney, M., Clayton, A., Smyth, L. A., Hider, R. C., et al. (2019) · Theranostics · doi:10.7150/thno.27891 · Cited by 141 · see citing works

Systematic review of targeted extracellular vesicles for drug delivery – Considerations on methodological and biological heterogeneity

Gudbergsson, J. M., Jønsson, K., Simonsen, J. B., & Johnsen, K. B. (2019) · Journal of Controlled Release · doi:10.1016/j.jconrel.2019.06.006 · Cited by 138 · see citing works

Exosomes as a novel way of interneuronal communication

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Harnessing exosomes for advanced osteoarthritis therapy

Selvadoss, A., Baby, H. M., Zhang, H., & Bajpayee, A. G. (2024) · Nanoscale · doi:10.1039/d4nr02792b · Cited by 32 · see citing works

An engineered M2 macrophage-derived exosomes-loaded electrospun biomimetic periosteum promotes cell recruitment, immunoregulation, and angiogenesis in bone regeneration

Wen, Z., Li, S., Liu, Y., Liu, X., Qiu, H., Che, Y., Bian, L., & Zhou, M. (2025) · Bioactive Materials · doi:10.1016/j.bioactmat.2025.03.027 · Cited by 30 · see citing works

Achievements and Bottlenecks of PEGylation in Nano-delivery Systems

Shen, R., & Yuan, H. (2022) · Current Medicinal Chemistry · doi:10.2174/0929867329666220929152644 · Cited by 29 · see citing works

DNA Nanomaterial‐Empowered Surface Engineering of Extracellular Vesicles

Yao, X., He, D., Wei, P., Niu, Z., Chen, H., Li, L., Fu, P., Wang, Y., Lou, S., Qian, S., et al. (2023) · Advanced Materials · doi:10.1002/adma.202306852 · Cited by 29 · see citing works

Bioengineering Outer-Membrane Vesicles for Vaccine Development: Strategies, Advances, and Perspectives

Zahid, A., Ismail, H., Wilson, J. C., & Grice, I. D. (2025) · Vaccines · doi:10.3390/vaccines13070767 · Cited by 29 · see citing works

Engineering Extracellular Microenvironment for Tissue Regeneration

Hao, D., Lopez, J.-M., Chen, J., Iavorovschi, A. M., Lelivelt, N. M., & Wang, A. (2022) · Bioengineering · doi:10.3390/bioengineering9050202 · Cited by 29 · see citing works

Drug delivery as a sustainable avenue to future therapies

Fuhrmann, G. (2023) · Journal of Controlled Release · doi:10.1016/j.jconrel.2023.01.045 · Cited by 28 · see citing works

Engineering challenges and translational opportunities in emerging gene delivery platforms

Ma, Y., Dong, S., Wu, A., Jeong, S. D., Lee, A. S., Jiang, W., & Kim, B. Y. S. (2026) · Nature Biomedical Engineering · doi:10.1038/s41551-026-01643-5 · Cited by 30 · see citing works

M1-Type Microglia-Derived Extracellular Vesicles Overexpressing IL-1R1 Promote Postoperative Cognitive Dysfunction by Regulating Neuronal Inflammation

Zheng, Q., Yu, Y., Su, Y., Cao, B., Shao, H., & Yang, J. (2023) · Inflammation · doi:10.1007/s10753-023-01875-6 · Cited by 28 · see citing works

Nanosystems for oxidative stress regulation in the anti-inflammatory therapy of acute kidney injury

Wang, Y., Jiang, H., Zhang, L., Yao, P., Wang, S., & Yang, Q. (2023) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2023.1120148 · Cited by 27 · see citing works

Extracellular vesicles as nanotheranostic platforms for targeted neurological disorder interventions

Choi, H. K., Chen, M., Goldston, L. L., & Lee, K.-B. (2024) · Nano Convergence · doi:10.1186/s40580-024-00426-5 · Cited by 26 · see citing works

New Therapeutics for Extracellular Vesicles: Delivering CRISPR for Cancer Treatment

Yan, B., & Liang, Y. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms232415758 · Cited by 28 · see citing works

Genetically Engineered Cellular Nanovesicles: Theories, Design and Perspective

Cheng, Q., Li, R., He, Y., Zhu, Y., Kang, Y., & Ji, X. (2024) · Advanced Functional Materials · doi:10.1002/adfm.202407842 · Cited by 26 · see citing works

Strategies for Small Extracellular Vesicle-Based Cancer Immunotherapy

Chen, Y., Tang, S., Cai, F., & Wan, Y. (2024) · Research · doi:10.34133/research.0421 · Cited by 25 · see citing works

Harnessing Bacterial Membrane Components for Tumor Vaccines: Strategies and Perspectives

Bai, Z., Wang, X., Liang, T., Xu, G., Cai, J., Xu, W., Yang, K., Hu, L. T., & Pei, P. (2024) · Advanced Healthcare Materials · doi:10.1002/adhm.202401615 · Cited by 25 · see citing works

Gene engineered exosome reverses T cell exhaustion in cancer immunotherapy

Li, P., Xie, Y., Wang, J., Bao, C., Duan, J., Liu, Y., Luo, Q., Xu, J., Ren, Y., Jiang, M., et al. (2024) · Bioactive Materials · doi:10.1016/j.bioactmat.2024.01.008 · Cited by 25 · see citing works

Engineered extracellular vesicles: an emerging nanomedicine therapeutic platform

Tang, J., Li, D., Wang, R., Li, S., Xing, Y., & Yu, F. (2025) · Chemical Communications · doi:10.1039/d4cc06501h · Cited by 24 · see citing works

A New Strategy to Functionalize Exosomes via Enzymatic Engineering of Surface Glycans and its Application to Profile Exosomal Glycans and Endocytosis

Kundu, S., Guo, J., Islam, M. S., Rohokale, R., Jaiswal, M., & Guo, Z. (2025) · Advanced Science · doi:10.1002/advs.202415942 · Cited by 24 · see citing works

Bioprobes-regulated precision biosensing of exosomes: From the nanovesicle surface to the inside

Peng, X., Qin, X., Qin, Y., Xiang, Y., Zhang, G., & Yang, F. (2022) · Coordination Chemistry Reviews · doi:10.1016/j.ccr.2022.214538 · Cited by 24 · see citing works

Lipid-hybrid cell-derived biomimetic functional materials: A state-of-the-art multifunctional weapon against tumors

Liu, W.-S., Wu, L., Chen, C.-M., Zheng, H., Gao, J., Lu, Z., & Li, M. (2023) · Materials Today Bio · doi:10.1016/j.mtbio.2023.100751 · Cited by 24 · see citing works

Extracellular vesicles: Emerged as a promising strategy for regenerative medicine

Wang, D., & Pan, J. (2023) · World Journal of Stem Cells · doi:10.4252/wjsc.v15.i4.165 · Cited by 24 · see citing works

Utilizing engineered extracellular vesicles as delivery vectors in the management of ischemic stroke: a special outlook on mitochondrial delivery

Chen, J., Li, Y., Quan, X., Chen, J., Han, Y., Li, Y., Zhou, M., Mok, G. S. P., Wang, R., & Zhao, Y. (2024) · Neural Regeneration Research · doi:10.4103/nrr.nrr-d-24-00243 · Cited by 23 · see citing works

Extracellular vesicles as nature's nano carriers in cancer therapy: Insights toward preclinical studies and clinical applications

Wu, X., Meng, Y., Yao, Z., Lin, X., Hu, M., Cai, S., Gao, S., & Zhang, H. (2025) · Pharmacological Research · doi:10.1016/j.phrs.2025.107751 · Cited by 25 · see citing works

Extracellular vesicles: A new frontier in deciphering the mechanisms of traditional Chinese medicine

Wang, K., He, S., Wang, Y., Guo, S., Zhang, F., Wang, Y., Dong, W., Zhang, L., Wang, X., & Yu, L. (2025) · Pharmacological Research · doi:10.1016/j.phrs.2025.107890 · Cited by 26 · see citing works

The potential therapeutic role of extracellular vesicles in critical-size bone defects: Spring of cell-free regenerative medicine is coming

Liu, F., Sun, T., An, Y., Ming, L., Li, Y., Zhou, Z., & Shang, F. (2023) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2023.1050916 · Cited by 34 · see citing works

Insight into extracellular vesicles in vascular diseases: intercellular communication role and clinical application potential

Liu, W., Jin, M., Chen, Q., Li, Q., Xing, X., Luo, Y., & Sun, X. (2023) · Cell Communication and Signaling · doi:10.1186/s12964-023-01304-z · Cited by 22 · see citing works

Therapeutics of the future: Navigating the pitfalls of extracellular vesicles research from an osteoarthritis perspective

Karoichan, A., Boucenna, S., & Tabrizian, M. (2024) · Journal of Extracellular Vesicles · doi:10.1002/jev2.12435 · Cited by 22 · see citing works

Adaptations of Bacterial Extracellular Vesicles in Response to Antibiotic Pressure

Dell’Annunziata, F., Cosimato, I., Salzano, F., Mensitieri, F., Andretta, V., Santoro, E., Boccia, G., Folliero, V., & Franci, G. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26115025 · Cited by 21 · see citing works

Extracellular vesicles as vehicles for small non-coding RNA therapeutics: standardization challenges for clinical translation

Luisotti, L., Germelli, L., Piccarducci, R., Giacomelli, C., Marchetti, L., & Martini, C. (2025) · Extracellular Vesicles and Circulating Nucleic Acids · doi:10.20517/evcna.2025.33 · Cited by 21 · see citing works

Natural Polyphenol-Functionalized Schwann Cell-Derived Exosomes as a Temporal Neuromodulation Strategy for Diabetic Periodontitis Therapy

Le, F., Wang, T., Liu, Y. Q., Ma, M., Liu, G., Wang, Y., Xia, J., Gu, Z., & Hao, Z. (2025) · ACS Nano · doi:10.1021/acsnano.5c08885 · Cited by 21 · see citing works

Mesenchymal Stromal Cell-Derived Extracellular Vesicles as Biological Carriers for Drug Delivery in Cancer Therapy

Sanmartin, M. C., Borzone, F. R., Giorello, M. B., Yannarelli, G., & Chasseing, N. A. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.882545 · Cited by 21 · see citing works

Recent advances in therapeutic engineered extracellular vesicles

Yao, C., Zhang, H., & Wang, C. (2024) · Nanoscale · doi:10.1039/d3nr05470e · Cited by 20 · see citing works

Engineered Bacterial Extracellular Vesicles: Developments, Challenges, and Opportunities

Li, Q., Chen, X., Xie, J., & Nie, S. (2025) · Engineering · doi:10.1016/j.eng.2025.06.042 · Cited by 20 · see citing works

Therapeutic potential of plant-derived exosome-like nanoparticles for CNS diseases: advancements in preparation and characterization

Liu, M., Wang, H., Yang, Y., Wang, J., Sun, Z., Zhao, S., Kong, W., Sun, W., Wang, C., & Feng, L. (2025) · Annals of Medicine · doi:10.1080/07853890.2025.2537345 · Cited by 24 · see citing works

Rational design of extracellular vesicles for targeted drug delivery across physiological barriers

Baby, H. M., Zhang, H., Selvadoss, A., Pathrikar, T. V., & Bajpayee, A. G. (2025) · Nano Today · doi:10.1016/j.nantod.2025.102920 · Cited by 21 · see citing works

Extracellular vesicles for targeted drug delivery: advances in surface modification strategies and therapeutic applications

Hu, M., Han, Y., Zhang, X., Tian, S., Shang, Z., Yuan, Z., & He, L. (2025) · Journal of Translational Medicine · doi:10.1186/s12967-025-07077-y · Cited by 46 · see citing works

A Chemical Framework for Engineering Extracellular Vesicles’ Biointerface to Advance Precision Therapeutics

Jahromi, L. P., Geng, F., Frenzke, J., & Fuhrmann, G. (2026) · Advanced Materials · doi:10.1002/adma.74203

Technologies for the Surface Functionalization of Extracellular Vesicles

Han, G., Geng, M., & Yin, H. (2024) · Springer eBooks · doi:10.1007/978-981-99-8365-0_11

Surface engineering of extracellular vesicles: trends, strategies, and applications in diagnosis and therapy

Hong, Y., Hu, S., Yu, Z., Yang, Y., & Yang, H. (2026) · Journal of Nanobiotechnology · doi:10.1186/s12951-026-04404-3 · Cited by 13 · see citing works

Targeted therapy using engineered extracellular vesicles: principles and strategies for membrane modification

Liu, Q., Li, D., Pan, X., & Liang, Y. (2023) · Journal of Nanobiotechnology · doi:10.1186/s12951-023-02081-0 · Cited by 201 · see citing works