Engineering hybrid exosomes by membrane fusion with liposomes
Open accessAbstract
Exosomes are a valuable biomaterial for the development of novel nanocarriers as functionally advanced drug delivery systems. To control and modify the performance of exosomal nanocarriers, we developed hybrid exosomes by fusing their membranes with liposomes using the freeze–thaw method. Exosomes embedded with a specific membrane protein isolated from genetically modified cells were fused with various liposomes, confirming that membrane engineering methods can be combined with genetic modification techniques. Cellular uptake studies performed using the hybrid exosomes revealed that the interactions between the developed exosomes and cells could be modified by changing the lipid composition or the properties of the exogenous lipids. These results suggest that the membrane-engineering approach reported here offers a new strategy for developing rationally designed exosomes as hybrid nanocarriers for use in advanced drug delivery systems.
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Exosomes are highly efficient in delivering cargo to cells due to their unique transmembrane proteins that promote cell attachment and facilitate endocytosis.
“On the other hand, exosomes are highly efficient in delivering cargo to cells due to their unique transmembrane proteins that promote cell attachment and facilitate endocytosis [113].”
From Exosome for mRNA delivery: Strategies and therapeutic applications (2024) · cites this paper as [113]
Exosome-liposome hybrids have been shown to form structures larger than 200 nm.
“These exosome-liposome hybrids have been shown to form structures larger than 200 nm [113]”
From Exosome for mRNA delivery: Strategies and therapeutic applications (2024) · cites this paper as [113]
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- Authors
- Sato, Y. T., Umezaki, K., Sawada, S., Mukai, S.-A., Sasaki, Y., Harada, N., Shiku, H., & Akiyoshi, K.
- Affiliations
- Kyoto University (JP)
- Mie University (JP)
- Corresponding author
- Akiyoshi, Kazunari
- Venue
- Scientific Reports
- Published
- 2016-02-25
- Volume
- 6
- Issue
- 1
- Pages
- 21933-21933
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC4766490
- OpenAlex
- W2406609064
- Cited by
- 744
- References
- 44
- Citations by year
- 2026: 67 · 2025: 101 · 2024: 108 · 2023: 118 · 2022: 102 · 2021: 89 · 2020: 66 · 2019: 34 · 2018: 35 · 2017: 18 · 2016: 5
- FWCI
- 15.93
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Institutional Repositories DataBase (IRDB) (submittedVersion, cc-by)
- Kyoto University Research Information Repository (Kyoto University) (submittedVersion, cc-by)
- Funders
- Japan Science and Technology Agency
- Exploratory Research for Advanced Technology
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- membrane fusion, liposomes, membrane engineering, drug delivery systems, cellular uptake, lipid composition, genetically modified cells, exogenous lipids
- MeSH terms
- RAW 264.7 Cells, Animals, Flow Cytometry, Fluorescent Dyes, Freezing, HeLa Cells, Humans, Liposomes, Membrane Fusion, Blotting, Western, Microscopy, Confocal, Erb-b2 Receptor Tyrosine Kinases, Fluorescence Resonance Energy Transfer, Mice, Nanoparticles, Exosomes
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