CoChat
Sign Up
Cited by 385Adipokines, Inflammation, and Metabolic DiseasesAdipose Tissue and MetabolismPeroxisome Proliferator-Activated Receptors

Chronic interleukin-6 (IL-6) treatment increased IL-6 secretion and induced insulin resistance in adipocyte: prevention by rosiglitazone

Lagathu, Claire, Bastard, Jean-Philippe, Auclair, Martine, Maachi, Mustapha, Capeau, Jacqueline, Caron, Martine·2003·Biochemical and Biophysical Research Communications
DOI 10.1016/j.bbrc.2003.10.013
Open this paper in CoChat — free account

Abstract

IL-6 has emerged as an important cytokine upregulated in states of insulin resistance such as type 2 diabetes. We evaluated the chronic effect of IL-6 on insulin signaling in 3T3-F442A and 3T3-L1 adipocytes. First, cells responded to a chronic treatment with IL-6 by initiating an autoactivation process that increased IL-6 secretion. Second, IL-6-treated adipocytes showed a decreased protein expression of IR-beta subunit and IRS-1 but also an inhibition of the insulin-induced activation of IR-beta, Akt/PKB, and ERK1/2. Moreover, IL-6 suppressed the insulin-induced lipogenesis and glucose transport consistent with a diminished expression of GLUT4. IL-6-treated adipocytes failed to maintain their adipocyte phenotype as shown by the downregulation of the adipogenic markers FAS, GAPDH, aP2, PPAR-gamma, and C/EBP-alpha. IL-6 also induced the expression of SOCS-3, a potential inhibitor of insulin signaling. Finally, the effects of IL-6 could be prevented by rosiglitazone, an insulin-sensitizing agent. Thus, IL-6 may play an important role in the set-up of insulin resistance in adipose cell.

Integrity signals

Registry

Crossref · 10.1016/j.bbrc.2003.10.013

Cites

42 of 42 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Gene expression of MCP-1, colony-stimulating factor-3 and urokinase plasminogen activator CD87 increases significantly in adipose tissue of subjects with morbid obesity.

    “Gene expression studies with human adipose tissue have demonstrated that the levels of expression of MCP-1, colony-stimulating factor-3 and the urokinase plasminogen activator CD87 increase significantly in the adipose tissue of subjects with morbid obesity (31) .”

    From Dietary factors and low-grade inflammation in relation to overweight and obesity (2011) · cites this paper as [31]

  • In obese subjects, macrophages typically aggregate in crowns around apoptotic adipocytes.

    “In obese subjects, these macrophages typically aggregate in crowns around apoptotic adipocytes (31) (Figs. 1 and 2).”

    From Dietary factors and low-grade inflammation in relation to overweight and obesity (2011) · cites this paper as [31]

  • HIF-1a is overexpressed in subcutaneous adipose tissue of obese subjects and decreases during weight reduction.

    “Hypoxia-inducible factor-1a, a transcription factor normally induced by hypoxia, is overexpressed in the subcutaneous adipose tissue of obese subjects and its expression is decreased during weight reduction (31) .”

    From Dietary factors and low-grade inflammation in relation to overweight and obesity (2011) · cites this paper as [31]

  • Increased IL-10 protein expression after weight loss suggests a possible M1 to M2 macrophage phenotype switch.

    “In this study, the protein expression of IL-10 increased, suggesting a possible M1 to M2 (pro-inflammatory to anti-inflammatory) switch of macrophage phenotypes (31) .”

    From Dietary factors and low-grade inflammation in relation to overweight and obesity (2011) · cites this paper as [31]

  • Reduced secretion of pro-inflammatory mediators from adipocytes or adipose tissue macrophages likely contributes to the effect of weight loss.

    “On the other hand, it seems likely that reduced secretion of pro-inflammatory mediators from adipocytes or activated macrophages of adipose tissue contributes to the effect of weight loss (31,36,65,67,142) .”

    From Dietary factors and low-grade inflammation in relation to overweight and obesity (2011) · cites this paper as [31]

Cites

Flagged first, then by citations

3 of the 45 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Related

Authors
Lagathu, C., Bastard, J.-P., Auclair, M., Maachi, M., Capeau, J., & Caron, M.
Affiliations
Inserm (FR)
Corresponding author
Caron, Martine
ORCID
Venue
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Published
2003-11
Volume
311
Issue
2
Pages
372-379
ISSN
0006-291X (ISSN-L)
DOI
10.1016/j.bbrc.2003.10.013
OpenAlex
W2061613314
Cited by
385
References
45
Citations by year
2026: 6 · 2025: 9 · 2024: 15 · 2023: 11 · 2022: 15 · 2021: 9 · 2020: 12 · 2019: 6 · 2018: 13 · 2017: 17 · 2016: 16 · 2015: 12 · 2014: 17 · 2013: 25 · 2012: 29
FWCI
16.14
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Biochemical and Biophysical Research Communications (publishedVersion)
  • PubMed (publishedVersion)
Funders
Institut National de la Santé et de la Recherche Médicale
Topics
  • Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
Keywords
interleukin-6, insulin resistance, adipocytes, rosiglitazone, insulin signaling, insulin receptor beta, IRS-1, Akt, ERK1/2, GLUT4, lipogenesis, adipogenesis, SOCS3, type 2 diabetes, 3T3-L1 cells
MeSH terms
Suppressor of Cytokine Signaling 3 Protein, Rosiglitazone, Animals, Dose-Response Relationship, Drug, Glucose, Insulin Resistance, Proteins, Repressor Proteins, Transcription Factors, Signal Transduction, Interleukin-6, Up-Regulation, 3T3 Cells, Adipocytes, 3T3-L1 Cells, Thiazolidinediones, Suppressor of Cytokine Signaling Proteins, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review