“Solvent-free” ultrasound-assisted extraction of lipids from fresh microalgae cells: A green, clean and scalable process
Open accessAbstract
In order to comply with criteria of green chemistry concepts and sustainability, a new procedure has been performed for solvent-free ultrasound-assisted extraction (UAE) to extract lipids from fresh Nannochloropsis oculata biomass. Through response surface methodology (RSM) parameters affecting the oil recovery were optimized. Optimum conditions for oil extraction were estimated as follows: (i) 1000 W ultrasonic power, (ii) 30 min extraction time and (iii) biomass dry weight content at 5%. Yields were calculated by the total fatty acids methyl esters amounts analyzed by GC-FID-MS. The maximum oil recovery was around 0.21%. This value was compared with the one obtained with the conventional extraction method (Bligh and Dyer). Furthermore, effect of temperature on the yield was also investigated. The overall results show an innovative and effective extraction method adapted for microalgae oil recovery, without using solvent and with an enable scaling up.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The major disadvantage of photobioreactors is high investment cost.
“In addition to these advantages, the major disadvantage of photobioreactors is high investment cost [34,35].”
From The Perspective of Large-Scale Production of Algae Biodiesel (2020) · cites this paper as [34]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751 · see citing works
Microalgae for biodiesel production and other applications: A review
Mata, T. M., Martins, A. A., & Caetano, N. S. (2010) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2009.07.020 · Cited by 5,685 · see citing works
Applications of ultrasound in food technology: Processing, preservation and extraction
Chemat, F., Zill-e-Huma, & Khan, M. K. (2010) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2010.11.023 · Cited by 2,541 · see citing works
Self-citation“Designer” Biodiesel: Optimizing Fatty Ester Composition to Improve Fuel Properties
Knothe, G. (2008) · Energy & Fuels · doi:10.1021/ef700639e · Cited by 1,291 · see citing works
Comparison of several methods for effective lipid extraction from microalgae
Lee, J.-Y., Yoo, C., Jun, S.-Y., Ahn, C.-Y., & Oh, H.-M. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2009.03.058 · Cited by 1,279 · see citing works
Biodiesel from algae: challenges and prospects
Scott, S. A., Davey, M. P., Dennis, J. S., Horst, I., Howe, C. J., Lea-Smith, D. J., & Smith, A. G. (2010) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2010.03.005 · Cited by 1,183 · see citing works
Commercialization potential of microalgae for biofuels production
Singh, J., & Gu, S. (2010) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2010.06.014 · Cited by 1,001 · cited 1× · see citing works
“Consequently, there is no competition with food production for a growing human population (Singh and Gu, 2010).”
BackgroundHandbook of Microalgal Culture. Biotechnology and Applied Phycology
Moi, P. S. (2004) · Journal of Applied Phycology · doi:10.1023/b:japh.0000044943.45511.5e · Cited by 920 · see citing works
Handbook of microalgal culture: biotechnology and applied phycology.
RICHMOND, A. E. (2004) · Cited by 861 · see citing works
Hydrothermal liquefaction and gasification of Nannochloropsis sp.
Brown, T. M., Duan, P., & Savage, P. E. (2010) · Energy & Fuels · doi:10.1021/ef100203u · Cited by 745 · see citing works
Oil extraction from microalgae for biodiesel production
Halim, R., Gladman, B., Danquah, M. K., & Webley, P. A. (2011) · Bioresource Technology · doi:10.1016/j.biortech.2010.06.136 · Cited by 683 · see citing works
Improved extraction of vegetable oils under high-intensity ultrasound and/or microwaves
Cravotto, G., Boffa, L., Mantegna, S., Perego, P., Avogadro, M., & Cintas, P. (2007) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2007.10.009 · Cited by 627 · see citing works
Developments in oil extraction from microalgae
Mercer, P., & Armenta, R. E. (2011) · European Journal of Lipid Science and Technology · doi:10.1002/ejlt.201000455 · Cited by 532 · see citing works
Oil content of Arabidopsis seeds: The influence of seed anatomy, light and plant-to-plant variation
Li‐Beisson, Y., Beisson, F., Pollard, M., et al. (2006) · Phytochemistry · doi:10.1016/j.phytochem.2006.02.015 · Cited by 404 · see citing works
Microwave-assisted pyrolysis of microalgae for biofuel production
Du, Z., Li, Y., Wang, X., Wan, Y., Chen, Q., Wang, C., Lin, X., Liu, Y., Chen, P. L., & Ruan, R. R. (2011) · Bioresource Technology · doi:10.1016/j.biortech.2011.01.055 · Cited by 391 · see citing works
Optimization of direct conversion of wet algae to biodiesel under supercritical methanol conditions
Patil, P. D., Gude, V. G., Mannarswamy, A., Deng, S., Cooke, P. H., Munson‐McGee, S. H., Rhodes, I., Lammers, P., & Nirmalakhandan, N. N. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2010.06.031 · Cited by 371 · see citing works
Balasubramanian, S., Allen, J. D., Kanitkar, A., & Boldor, D. (2011) · Bioresource Technology · doi:10.1016/j.biortech.2010.09.119 · Cited by 283 · see citing works
Mechanistic Assessment of Microalgal Lipid Extraction
Ranjan, A., Patil, C., & Moholkar, V. S. (2010) · Industrial & Engineering Chemistry Research · doi:10.1021/ie9016557 · Cited by 234 · see citing works
Supercritical fluid extraction of bioactive lipids from the microalga Nannochloropsis sp.
Andrich, G., Nesti, U., Venturi, F., Zinnai, A., & Fiorentini, R. (2005) · European Journal of Lipid Science and Technology · doi:10.1002/ejlt.200501130 · Cited by 181 · see citing works
Practical sonochemistry : users guide to applications in chemistry and chemical engineering
Mason, T. J. (1991) · Pure (Coventry University) · Cited by 179 · see citing works
Hu, H., & Gao, K. (2003) · Biotechnology Letters · doi:10.1023/a:1022489108980 · Cited by 166 · see citing works
Mason, J. W. (1990) · Medical Entomology and Zoology · Cited by 145 · see citing works
Krienitz, L., & Wirth, M. (2006) · Limnologica · doi:10.1016/j.limno.2006.05.002 · Cited by 133 · see citing works
Characterization of Lipid Components in Two Microalgae for Biofuel Application
Wang, G., & Wang, T. (2011) · Journal of the American Oil Chemists Society · doi:10.1007/s11746-011-1879-8 · Cited by 118 · see citing works
Churchward, M. A., Brandman, D. M., Rogasevskaia, T. P., & Coorssen, J. R. (2008) · Journal of Chemical Biology · doi:10.1007/s12154-008-0007-1 · Cited by 56 · see citing works
Enzymatic Degradation of Microalgal Cell Walls
Sander, K. B., & Murthy, G. S. (2009) · 2009 Reno, Nevada, June 21 - June 24, 2009 · doi:10.13031/2013.27044 · Cited by 41 · see citing works
Gottel, M., Eing, C., Gusbeth, C., Frey, W., & Müller, G. (2011) · IEEE International Conference on Plasma Science (ICOPS) · doi:10.1109/plasma.2011.5993394 · Cited by 11 · see citing works
Wiyarno, B., Yunus, R. M., & Mel, M. (2010) · Cited by 6 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Chemat, F., Rombaut, N., Sicaire, A.-G., Meullemiestre, A., Fabiano-Tixier, A.-S., & Abert-Vian, M. (2017) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2016.06.035 · Cited by 3,278 · see citing works
Self-citationMicroalgae biorefinery: High value products perspectives
Chew, K. W., Yap, J. Y., Show, P. L., Suan, N. H., Juan, J. C., Ling, T. C., Lee, D.-J., & Chang, J.-S. (2017) · Bioresource Technology · doi:10.1016/j.biortech.2017.01.006 · Cited by 1,336 · see citing works
Ultrasound: A clean, green extraction technology
Tiwari, B. K. (2015) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2015.04.013 · Cited by 1,042 · see citing works
Methods of downstream processing for the production of biodiesel from microalgae
Kim, J., Yoo, G., Lee, H., Lim, J., Kim, K., Kim, C. W., Park, M. S., & Yang, J.-W. (2013) · Biotechnology Advances · doi:10.1016/j.biotechadv.2013.04.006 · Cited by 519 · see citing works
Lipid Extraction Methods from Microalgae: A Comprehensive Review
Ranjith Kumar, R., Hanumantha Rao, P., & Arumugam, M. (2015) · Frontiers in Energy Research · doi:10.3389/fenrg.2014.00061 · Cited by 402 · see citing works
Carreira-Casais, A., Otero, P., Garcia-Perez, P., Garcia-Oliveira, P., Pereira, A. G., Carpena, M., Soria-Lopez, A., Simal-Gandara, J., & Prieto, M. A. (2021) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph18179153 · Cited by 395 · see citing works
Lipid recovery from wet oleaginous microbial biomass for biofuel production: A critical review
Dong, T., Knoshaug, E. P., Pienkos, P. T., & Laurens, L. M. L. (2016) · Applied Energy · doi:10.1016/j.apenergy.2016.06.002 · Cited by 349 · see citing works
The potential of microalgae in biodiesel production
Chen, J., Li, J., Dong, W., Zhang, X., Tyagi, R. D., Drogui, P., & Surampalli, R. Y. (2018) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2018.03.073 · Cited by 330 · see citing works
A Review: Microalgae and Their Applications in CO2 Capture and Renewable Energy
Klinthong, W., Yang, Y.-H., Huang, C.-H., & Tan, C.-S. (2015) · Aerosol and Air Quality Research · doi:10.4209/aaqr.2014.11.0299 · Cited by 278 · see citing works
Algae as green energy reserve: Technological outlook on biofuel production
Anto, S., Mukherjee, S. S., Muthappa, R., Mathimani, T., Deviram, G., Kumar, S. S., Verma, T. N., & Pugazhendhi, A. (2020) · Chemosphere · doi:10.1016/j.chemosphere.2019.125079 · Cited by 277 · see citing works
Alternative and efficient extraction methods for marine-derived compounds
Grosso, C., Valentão, P., Ferreres, F., & Andrade, P. (2015) · Marine Drugs · doi:10.3390/md13053182 · Cited by 248 · see citing works
Panda, D., & Manickam, S. (2019) · Applied Sciences · doi:10.3390/app9040766 · Cited by 239 · see citing works
Muhammad, G., Alam, M. A., Mofijur, M., Jahirul, M. I., Lv, Y., Xiong, W., Ong, H. C., & Xu, J. (2021) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2020.110209 · Cited by 226 · see citing works
The Perspective of Large-Scale Production of Algae Biodiesel
Bošnjaković, M., & Sinaga, N. (2020) · Applied Sciences · doi:10.3390/app10228181 · Cited by 194 · see citing works
Koyande, A. K., Show, P.-L., Guo, R., Tang, B., Ogino, C., & Chang, J.-S. (2019) · Bioengineered · doi:10.1080/21655979.2019.1679697 · Cited by 182 · see citing works
RetractedGreen technology for the industrial production of biofuels and bioproducts from microalgae: A review
Tang, D. Y. Y., Yew, G. Y., Koyande, A. K., Chew, K. W., Vo, D.-V. N., & Show, P. L. (2020) · Environmental Chemistry Letters · doi:10.1007/s10311-020-01052-3 · Cited by 142 · see citing works
Techniques of lipid extraction from microalgae for biofuel production: a review
Lee, S. Y., Khoiroh, I., Vo, D.-V. N., Senthil Kumar, P., & Show, P. L. (2021) · Environmental Chemistry Letters · doi:10.1007/s10311-020-01088-5 · Cited by 133 · see citing works
Biofuels from Microalgae Biomass: A Review of Conversion Processes and Procedures
Ebhodaghe, S. O., Imanah, O. E., & Ndibe, H. (2022) · Arabian Journal of Chemistry · doi:10.1016/j.arabjc.2021.103591 · Cited by 93 · see citing works
Advances in microalgal lipid extraction for biofuel production: a review
Harris, J., Viner, K., Champagne, P., & Jessop, P. G. (2018) · Biofuels, Bioproducts and Biorefining · doi:10.1002/bbb.1923 · Cited by 64 · see citing works
Dong, T., Van Wychen, S., Nagle, N., Pienkos, P. T., & Laurens, L. M. L. (2016) · Algal Research · doi:10.1016/j.algal.2016.06.004 · Cited by 54 · see citing works
Liquid By-Products from Fish Canning Industry as Sustainable Sources of Ω3 Lipids
Monteiro, A., Paquincha, D., Martins, F., Queirós, R. P., Saraiva, J. A., Švarc-Gajić, J., Nastić, N., Delerue-Matos, C., & Carvalho, A. P. (2018) · Journal of Environmental Management · doi:10.1016/j.jenvman.2018.04.102 · Cited by 36 · see citing works
Green analytical methodologies for the discovery of bioactive compounds from marine sources
Duarte, K., Justino, C. I. L., Pereira, R., Freitas, A. C., Gomes, A. M., Duarte, A. C., & Rocha-Santos, T. A. P. (2014) · Trends in Environmental Analytical Chemistry · doi:10.1016/j.teac.2014.11.001 · Cited by 21 · see citing works
Algae Biomass: Importance, Harvesting Techniques, Extraction Methods, and Associated Challenges
Gautam, R., Patial, S. K., & Singh, S. (2024) · Value Added Products From Bioalgae Based Biorefineries: Opportunities and Challenges · doi:10.1007/978-981-97-1662-3_4 · Cited by 13 · see citing works
- Authors
- Adam, F., Abert-Vian, M., Peltier, G., & Chemat, F.
- Affiliations
- Université d'Avignon et des Pays de Vaucluse (FR)
- Centre National de la Recherche Scientifique (FR)
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2012-06
- Volume
- 114
- Pages
- 457-465
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W2026661310
- Cited by
- 317
- References
- 28
- Citations by year
- 2026: 12 · 2025: 15 · 2024: 16 · 2023: 21 · 2022: 22 · 2021: 32 · 2020: 36 · 2019: 25 · 2018: 32 · 2017: 31 · 2016: 16 · 2015: 23 · 2014: 19 · 2013: 14 · 2012: 3
- FWCI
- 7.23
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- HAL AMU (submittedVersion, other-oa)
- Funders
- Institut National de la Recherche Agronomique
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Engineering › Biomedical Engineering › Biodiesel Production and Applications
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Keywords
- ultrasound-assisted extraction, solvent-free extraction, microalgal lipids, Nannochloropsis oculata, green chemistry, fatty acid methyl esters, extraction temperature, extraction optimization, Bligh and Dyer method, scale-up
- MeSH terms
- Pilot Projects, Solvents, Sonication, Green Chemistry Technology, Microalgae
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review