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Cited by 93Neurogenesis and neuroplasticity mechanismsSleep and Wakefulness ResearchMemory and Neural Mechanisms

Sleep restriction by forced activity reduces hippocampal cell proliferation

Roman, Viktor, Van der Borght, Karin, Leemburg, Susan A., Van der Zee, Eddy A., Meerlo, Peter·2005·Brain Research
DOI 10.1016/j.brainres.2005.10.020
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Abstract

Mounting evidence suggests that sleep loss negatively affects learning and memory processes through disruption of hippocampal function. In the present study, we examined whether sleep loss alters the generation, differentiation, and survival of new cells in the dentate gyrus. Rats were sleep restricted by keeping them awake in slowly rotating drums for 1 day or repeatedly for 20 h/day over a period of 8 days. In addition to home cage controls, we included forced activity controls which, compared to sleep restricted rats, walked at double speed for half the time. These animals thus walked the same distance but had sufficient time to sleep. The results show that a single day of sleep deprivation significantly reduced hippocampal cell proliferation in the hilus of the dentate gyrus as measured by immunostaining for the proliferation marker Ki-67. Repeated partial sleep deprivation reduced cell proliferation in both the hilus and the subgranular zone. However, the latter was also found after chronic forced activity, and may not have been specific for sleep loss. To study neuronal survival, rats received a single intraperitoneal injection of 5-bromo-2'-deoxyuridine (BrdU) 5 days before the experiment. The number of surviving, BrdU-positive cells was not affected by sleep restriction. Also, the differentiation of BrdU-positive new cells into NeuN-positive neuronal and GFAP-positive glial phenotypes was not significantly altered by sleep loss. In conclusion, since new cells in the hilus mostly differentiate into glia, our findings indicate that sleep loss may reduce hippocampal gliogenesis.

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  • Keeping rats in a slowly rotating drum has also been reported to produce a similar neural effect of suppressed cell proliferation.

    “A similar neural effect has also been reported by keeping rats in a slowly rotating drum”

    From Sleep Deprivation and Circadian Disruption (2015) · cites this paper as [76]

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Authors
Roman, V., Van der Borght, K., Leemburg, S. A., Van der Zee, E. A., & Meerlo, P.
Affiliations
University of Groningen (NL)
Corresponding author
Meerlo, Peter
ORCID
Venue
Brain Research
Publisher
Elsevier BV
Published
2005-12
Volume
1065
Issue
1-2
Pages
53-59
ISSN
0006-8993 (ISSN-L)
DOI
10.1016/j.brainres.2005.10.020
OpenAlex
W1983686148
Cited by
93
References
33
Citations by year
2026: 2 · 2025: 2 · 2024: 4 · 2023: 2 · 2022: 3 · 2021: 3 · 2020: 2 · 2019: 2 · 2018: 3 · 2017: 4 · 2016: 4 · 2015: 7 · 2014: 10 · 2013: 11 · 2012: 3
FWCI
1.23
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Brain Research (publishedVersion)
  • PubMed (publishedVersion)
  • University of Groningen research database (University of Groningen / Centre for Information Technology) (publishedVersion)
  • University of Groningen research database (University of Groningen / Centre for Information Technology) (submittedVersion)
Topics
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Sleep and Wakefulness Research
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
Keywords
sleep deprivation, hippocampal cell proliferation, dentate gyrus, gliogenesis, neuronal differentiation, Ki-67, bromodeoxyuridine, subgranular zone, rotating drum
MeSH terms
Animals, Antimetabolites, Bromodeoxyuridine, Glial Fibrillary Acidic Protein, Hippocampus, Immunohistochemistry, Male, Motor Activity, Perfusion, Phenotype, Physical Stimulation, Sleep Deprivation, Biomarkers, Rats, Wistar, Ki-67 Antigen, Cell Proliferation, Rats

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