A sulfated polysaccharide, fucans, isolated from brown algae Sargassum vulgare with anticoagulant, antithrombotic, antioxidant and anti-inflammatory effects
Abstract
Fucan (SV1) sulfated polysaccharides from the brown algae Sargassum vulgare were extracted, fractionated in acetone and examined with respect to chemical composition, anticoagulant, anti-inflammatory, antithrombotic effects and cellular proliferation. These polysaccharides contain low levels of protein, high level of carbohydrate and sulfate. Monosaccharides analysis revealed that SV1 was composed of fucose, galactose, xylose, glucuronic acid and mannose. SV1 polysaccharide prolonged activated partial thromboplastin time (aPTT) and exhibited high antithrombotic action in vivo, with a concentration ten times higher than heparin activity. PSV1, a purified form in gel filtration showed very low biological activities. SV1 stimulated the enzymatic activity of FXa. Its action on DPPH radical scavenging activity was 22%. This polymer has no cytotoxic action (hemolytic) on ABO and Rh blood types in different erythrocyte groups. It displays strong anti-inflammatory action at all concentrations tested in the carrageenan-induced paw edema model, demonstrated by reduced edema and cellular infiltration.
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Protease enzymes have been used to isolate fucoidan with anticoagulant, antithrombotic, antioxidant and antiinflammatory effects from Sargassum vulgare, followed by acetone precipitation.
“The use of protease enzymes was described for the isolation of fucoidan with anticoagulant, antithrombotic, antioxidant and antiinflammatory effects from Sargassum vulgare biomass followed by solvent precipitation with acetone”
From Polysaccharides from macroalgae: Recent advances, innovative technologies and challenges in extraction and purification (2017) · cites this paper as Dore et al., 2013
O-sulphated 3-linked α-galactans and 3-O-sulphation at C-3 of 4-α-L-fucose-1→ units in fucoidan are mainly responsible for anticoagulant activity.
“O-sulphated 3-linked αgalactans and 3-O-sulphation at C-3 of 4-α-L-fucose-1 → units (in fucoidan) is mainly responsible for its anticoagulant activity”
From Polysaccharides from macroalgae: Recent advances, innovative technologies and challenges in extraction and purification (2017) · cites this paper as Dore et al., 2013
Macroalgal polysaccharides display antiinflammatory, immunostimulatory, antioxidant, anticoagulant, antimicrobial, antiviral, antipeptic, antiadhesive, antiproliferative, antiapoptosis and antitumour properties.
“Macroalgal polysaccharides display a wide range of biological properties including antiinflammatory, immunostimulatory, antioxidant, anticoagulant, antimicrobial, antiviral, antipeptic, antiadhesive, antiproliferative, antiapoptosis and antitumour properties”
From Polysaccharides from macroalgae: Recent advances, innovative technologies and challenges in extraction and purification (2017) · cites this paper as Dore et al., 2013
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- Authors
- Dore, C. M. P. G., Faustino Alves, M. G. D. C., Pofírio Will, L. S. E., Costa, T. G., Sabry, D. A., de Souza Rêgo, L. A. R., Accardo, C. M., Rocha, H. A. O., Filgueira, L. G. A., & Leite, E. L.
- Affiliations
- Universidade Federal do Rio Grande do Norte (BR)
- Universidade Federal de São Paulo (BR)
- Corresponding author
- Leite, Edda Lisboa
- ORCID
- Venue
- Carbohydrate Polymers
- Publisher
- Elsevier BV
- Published
- 2013-01
- Volume
- 91
- Issue
- 1
- Pages
- 467-475
- ISSN
- 0144-8617 (ISSN-L)
- OpenAlex
- W2021800696
- Cited by
- 286
- References
- 32
- Citations by year
- 2026: 5 · 2025: 22 · 2024: 23 · 2023: 18 · 2022: 26 · 2021: 29 · 2020: 35 · 2019: 26 · 2018: 22 · 2017: 19 · 2016: 20 · 2015: 15 · 2014: 11 · 2013: 15
- FWCI
- 19.28
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Carbohydrate Polymers (publishedVersion)
- PubMed (publishedVersion)
- UNIFESP Institutional Repository (Universidade Federal de São Paulo) (submittedVersion)
- Funders
- CAPES
- MCT
- FAPERN/CNPq
- CNPq/Brazil
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
- Keywords
- fucan, sulfated polysaccharides, Sargassum vulgare, brown algae, anticoagulant activity, antithrombotic activity, antioxidant activity, anti-inflammatory activity, activated partial thromboplastin time, factor Xa, DPPH radical scavenging, hemolytic activity, carrageenan-induced paw edema, polysaccharide purification
- MeSH terms
- ABO Blood-Group System, Animals, Anti-Inflammatory Agents, Non-Steroidal, Anticoagulants, Carrageenan, Cell Survival, Edema, Erythrocytes, Fibrinolytic Agents, Hemolysis, Male, Polysaccharides, Rh-Hr Blood-Group System, Thrombin, Factor Xa, Free Radical Scavengers, Rats, Wistar, Sargassum, Cell Line, Tumor, Mice, Rats
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