Cited by 580Wnt/β-catenin signaling in development and cancerDevelopmental Biology and Gene RegulationHedgehog Signaling Pathway Studies

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Secretion of Wnt Ligands Requires Evi, a Conserved Transmembrane Protein

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Bartscherer, Kerstin, Pelte, Nadège, Ingelfinger, Dierk, Boutros, Michael·2006·Cell

DOI 10.1016/j.cell.2006.04.009

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Abstract

Wnt signaling pathways are important for multiple biological processes during development and disease. Wnt proteins are secreted factors that activate target-gene expression in both a short- and long-range manner. Currently, little is known about how Wnts are released from cells and which factors facilitate their secretion. Here, we identify a conserved multipass transmembrane protein, Evenness interrupted (Evi/Wls), through an RNAi survey for transmembrane proteins involved in Drosophila Wingless (Wg) signaling. During development, evi mutants have patterning defects that phenocopy wg loss-of-function alleles and fail to express Wg target genes. evi's function is evolutionarily conserved as depletion of its human homolog disrupts Wnt signaling in human cells. Epistasis experiments and clonal analysis place evi in the Wg-producing cell. Our results show that Wg is retained by evi mutant cells and suggest that evi is the founding member of a gene family specifically required for Wg/Wnt secretion.

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Authors: Bartscherer, K., Pelte, N., Ingelfinger, D., & Boutros, M.
Affiliations: German Cancer Research Center (DE)
Corresponding author: Boutros, Michael
ORCID: Bartscherer, Kerstin
Boutros, Michael
Venue: Cell
Publisher: Elsevier BV
Published: 2006-05
Volume: 125
Issue: 3
Pages: 523-533
ISSN: 0092-8674 (ISSN-L)
DOI: 10.1016/j.cell.2006.04.009
OpenAlex: W2067937471
Cited by: 580
References: 53
Citations by year: 2026: 14 · 2025: 13 · 2024: 21 · 2023: 20 · 2022: 24 · 2021: 31 · 2020: 33 · 2019: 34 · 2018: 35 · 2017: 33 · 2016: 35 · 2015: 29 · 2014: 45 · 2013: 29 · 2012: 37
FWCI: 12.43
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/open-access/userlicense/1.0/ (vor, 2634 d)
Locations: Cell (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Hedgehog Signaling Pathway Studies
Keywords: Wnt secretion, Wnt signaling, Drosophila, transmembrane proteins, gene family evolution, epistasis, clonal analysis, RNAi screen, developmental patterning
MeSH terms: Animals, Cell Line, Cells, Cultured, Clone Cells, Drosophila melanogaster, Humans, Membrane Proteins, Mutation, Phenotype, Signal Transduction, Conserved Sequence, Gene Expression Regulation, Developmental, Cell Lineage, Evolution, Molecular, Body Patterning, Drosophila Proteins, RNA Interference, Intracellular Signaling Peptides and Proteins, Wnt Proteins

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Secretion of Wnt Ligands Requires Evi, a Conserved Transmembrane Protein

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