Fourth generation biofuel from genetically modified algal biomass: Challenges and future directions.

Velvet: Algorithms for de novo short read assembly using de Bruijn graphsPreprint

Zerbino, D. R., & Birney, E. (2008) · Genome Research · doi:10.1101/gr.074492.107 · Cited by 9,587

Biodiesel from microalgae

Chisti, Y. (2007) · Biotechnology Advances · doi:10.1016/j.biotechadv.2007.02.001 · Cited by 9,524

The new frontier of genome engineering with CRISPR-Cas9

Doudna, J. A., & Charpentier, E. (2014) · Science · doi:10.1126/science.1258096 · Cited by 7,062

CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes

Barrangou, R., Fremaux, C., Deveau, H., Richards, M., Boyaval, P., Moineau, S., Romero, D. A., & Horvath, P. (2007) · Science · doi:10.1126/science.1138140 · Cited by 5,947

Ammonia as a case study for the spontaneous ionization of a simple hydrogen-bonded compound

Palasyuk, T., Troyan, I. A., Eremets, M. I., Drozd, V., Medvedev, S. A., Zaleski‐Ejgierd, P., Magos–Palasyuk, E., Wang, H., Bonev, S., Dudenko, D., et al. (2014) · Nature Communications · doi:10.1038/ncomms4460 · Cited by 3,235

Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation

Gilbert, L. A., Horlbeck, M. A., Adamson, B., Villalta, J. E., Chen, Y., Whitehead, E. H., Guimaraes, C., Panning, B., Ploegh, H. L., Bassik, M. C., et al. (2014) · Cell · doi:10.1016/j.cell.2014.09.029 · Cited by 2,728

Comparison of Next-Generation Sequencing Systems

Liu, L., Li, Y., Li, S., Hu, N., He, Y., Pong, R., Lin, D., Lü, L., & Law, M. (2012) · BioMed Research International · doi:10.1155/2012/251364 · Cited by 1,605

Programming cells by multiplex genome engineering and accelerated evolution

Wang, H. H., Isaacs, F. J., Carr, P. A., Sun, Z. Z., Xu, G., Forest, C. R., & Church, G. M. (2009) · Nature · doi:10.1038/nature08187 · Cited by 1,529

Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities

Sanson, K. R., Hanna, R. E., Hegde, M., Donovan, K. F., Strand, C., Sullender, M. E., Vaimberg, E. W., Goodale, A., Root, D. E., Piccioni, F., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-07901-8 · Cited by 1,043

Horizontal gene transfer, genome innovation and evolution

Gogarten, J. P., & Townsend, J. P. (2005) · Nature Reviews Microbiology · doi:10.1038/nrmicro1204 · Cited by 1,035

Synthesis of clathrate cerium superhydride CeH9 at 80-100 GPa with atomic hydrogen sublattice

Salke, N. P., Esfahani, M. M. D., Zhang, Y., Kruglov, I. A., Zhou, J., Wang, Y., Greenberg, E., Prakapenka, V. B., Liu, J., Оганов, А. Р., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-12326-y · Cited by 1,020

A Guild of 45 CRISPR-Associated (Cas) Protein Families and Multiple CRISPR/Cas Subtypes Exist in Prokaryotic Genomes

Haft, D. H., Selengut, J., Mongodin, E. F., & Nelson, K. E. (2005) · PLoS Computational Biology · doi:10.1371/journal.pcbi.0010060 · Cited by 1,014

BiGG Models: A platform for integrating, standardizing and sharing genome-scale models

King, Z. A., Lu, J., Dräger, A., Miller, P., Federowicz, S., Lerman, J. A., Ebrahim, A., Palsson, B. O., & Lewis, N. E. (2016) · Nucleic Acids Research · doi:10.1093/nar/gkv1049 · Cited by 938

A split-Cas9 architecture for inducible genome editing and transcription modulation

Zetsche, B., Volz, S. E., & Zhang, F. (2015) · Nature Biotechnology · doi:10.1038/nbt.3149 · Cited by 722

SUPPA2: fast, accurate, and uncertainty-aware differential splicing analysis across multiple conditions

Trincado, J. L., Entizne, J. C., Hysenaj, G., Singh, B., Škalič, M., Elliott, D. J., & Eyras, E. (2018) · Genome biology · doi:10.1186/s13059-018-1417-1 · Cited by 720

A system for the continuous directed evolution of biomolecules

Esvelt, K. M., Carlson, J. C., & Liu, D. R. (2011) · Nature · doi:10.1038/nature09929 · Cited by 694

Assemblathon 2: evaluating de novo methods of genome assembly in three vertebrate species

Bradnam, K. R., Fass, J., Alexandrov, A., Baranay, P., Bechner, M., Birol, İ., Boisvert, S., Chapman, J., Chapuis, G., Chikhi, R., et al. (2013) · GigaScience · doi:10.1186/2047-217x-2-10 · Cited by 658

Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production

Blazeck, J., Hill, A. D., Liu, L., Knight, R., Miller, J., Pan, A., Otoupal, P. B., & Alper, H. S. (2014) · Nature Communications · doi:10.1038/ncomms4131 · Cited by 587

Precise Manipulation of Chromosomes in Vivo Enables Genome-Wide Codon Replacement

Isaacs, F. J., Carr, P. A., Wang, H. H., Lajoie, M. J., Sterling, B., Kraal, L., Tolonen, A. C., Gianoulis, T. A., Goodman, D. B., Reppas, N. B., et al. (2011) · Science · doi:10.1126/science.1205822 · Cited by 557

Correction: Corrigendum: A subpopulation of high IL-21-producing CD4+ T cells in Peyer’s Patches is induced by the microbiota and regulates germinal centers

Jones, L. A., Ho, W. Q., Sze, Y., Ramakrishna, L., Srinivasan, K. G., Yurieva, M., Ng, W. P., Subramaniam, S., Hamadee, N. H., Joseph, S. S., et al. (2016) · Scientific Reports · doi:10.1038/srep34899 · Cited by 552

Biological containment of genetically modified Lactococcus lactis for intestinal delivery of human interleukin 10

Steidler, L., Neirynck, S., Huyghebaert, N., Snoeck, V., Vermeire, A., Goddeeris, B. M., Cox, E., Remon, J. P., & Remaut, E. (2003) · Nature Biotechnology · doi:10.1038/nbt840 · Cited by 545

Fourth generation biofuel: A review on risks and mitigation strategies.

Abdullah, B., Syed Muhammad, S. A. F., Shokravi, Z., Ismail, S., Kassim, K. A., Mahmood, A. N., & Aziz, M. M. A. (2019) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2019.02.018 · Cited by 531

Author Correction: The nature of spin excitations in the one-third magnetization plateau phase of Ba3CoSb2O9

Kamiya, Y., Ge, L., Hong, T., Qiu, Y., Quintero-Castro, D. L., Lu, Z., Cao, H. B., Matsuda, M., Choi, E. S., Batista, C. D., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-05679-3 · Cited by 529

The Chlorella variabilis NC64A Genome Reveals Adaptation to Photosymbiosis, Coevolution with Viruses, and Cryptic Sex

Blanc, G., Duncan, G. A., Agarkova, I., Borodovsky, M. Y., Gurnon, J. R., Kuo, A., Lindquist, E., Lucas, S. M., Pangilinan, J. L., Polle, J. E. W., et al. (2010) · The Plant Cell · doi:10.1105/tpc.110.076406 · Cited by 504

CRISPR/Cas9 Platforms for Genome Editing in Plants: Developments and Applications

Ma, X., Zhu, Q., Chen, Y., & Liu, Y. (2016) · Molecular Plant · doi:10.1016/j.molp.2016.04.009 · Cited by 497

Biocontainment of genetically modified organisms by synthetic protein design

Mandell, D. J., Lajoie, M. J., Mee, M. T., Takeuchi, R., Kuznetsov, G., Norville, J. E., Gregg, C. J., Stoddard, B. L., & Church, G. M. (2015) · Nature · doi:10.1038/nature14121 · Cited by 487

Metabolic engineering of Escherichia coli using CRISPR–Cas9 meditated genome editing

Li, Y., Lin, Z., Huang, C., Zhang, Y., Wang, Z., Ya-jie Tang, A., Chen, T., & Zhao, X. (2015) · Metabolic Engineering · doi:10.1016/j.ymben.2015.06.006 · Cited by 480

Sequence assembly demystified

Nagarajan, N., & Pop, M. (2013) · Nature Reviews Genetics · doi:10.1038/nrg3367 · Cited by 474

The CRISPR-Cas immune system: Biology, mechanisms and applications

Rath, D., Amlinger, L., Rath, A., & Lundgren, M. (2015) · Biochimie · doi:10.1016/j.biochi.2015.03.025 · Cited by 471

A field guide to whole‐genome sequencing, assembly and annotation

Ekblom, R., & Wolf, J. B. W. (2014) · Evolutionary Applications · doi:10.1111/eva.12178 · Cited by 470

Enhanced lipid production of Chlorella vulgaris by adjustment of cultivation conditions

Lv, J.-M., Cheng, L., Xu, X., Zhang, L., & Chen, H. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2010.03.120 · Cited by 464

Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology

Daboussi, F., Leduc, S., Maréchal, A., Dubois, G., Guyot, V., Perez-Michaut, C., Amato, A., Falciatore, A., Juillerat, A., Beurdeley, M., et al. (2014) · Nature Communications · doi:10.1038/ncomms4831 · Cited by 455

CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii

Shin, S.-E., Lim, J., Koh, H. G., Kim, E. K., Kang, N. K., Jeon, S., Kwon, S., Shin, W.-S., Lee, B., Hwangbo, K., et al. (2016) · Scientific Reports · doi:10.1038/srep27810 · Cited by 438

Inhibition of starch synthesis results in overproduction of lipids in Chlamydomonas reinhardtii

Li, Y., Han, D., Hu, G.-R., Sommerfeld, M. R., & Hu, Q. (2010) · Biotechnology and Bioengineering · doi:10.1002/bit.22807 · Cited by 431

A CRISPR/Cas9 system adapted for gene editing in marine algae

Nymark, M., Sharma, A. K., Sparstad, T., Bones, A. M., & Winge, P. (2016) · Scientific Reports · doi:10.1038/srep24951 · Cited by 413

Chlamydomonas starchless mutant defective in ADP-glucose pyrophosphorylase hyper-accumulates triacylglycerol

Li, Y., Han, D., Hu, G., Dauvillée, D., Sommerfeld, M. R., Ball, S. G., & Hu, Q. (2010) · Metabolic Engineering · doi:10.1016/j.ymben.2010.02.002 · Cited by 384

Metabolic engineering of algae for fourth generation biofuels production

Lu, J. J., Sheahan, C., & Fu, P. (2011) · Energy & Environmental Science · doi:10.1039/c0ee00593b · Cited by 383

Plant genome sequencing: applications for crop improvement

Edwards, D., & Batley, J. (2009) · Plant Biotechnology Journal · doi:10.1111/j.1467-7652.2009.00459.x · Cited by 364

CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window

Halperin, S. O., Tou, C. J., Wong, E. B., Modavi, C., Schaffer, D. V., & Dueber, J. E. (2018) · Nature · doi:10.1038/s41586-018-0384-8 · Cited by 348

Assessment of the safety of foods derived from genetically modified (GM) crops

König, A., Cockburn, A., Crevel, R., Debruyne, E., Grafstroem, R., Hammerling, U., Kimber, I., Knudsen, I., Kuiper, H. A., Peijnenburg, A., et al. (2004) · Food and Chemical Toxicology · doi:10.1016/j.fct.2004.02.019 · Cited by 347

Genetic improvement of the microalga Phaeodactylum tricornutum for boosting neutral lipid accumulation

Xue, J., Niu, Y.-F., Huang, T., Yang, W.-D., Liu, J.-S., & Li, H.-Y. (2015) · Metabolic Engineering · doi:10.1016/j.ymben.2014.10.002 · Cited by 321

GM crops: global socio-economic and environmental impacts 1996- 2006

Brookes, G., & Barfoot, P. (2008) · Cited by 320

Perspectives on engineering strategies for improving biofuel production from microalgae — A critical review

Ho, S., Ye, X., Hasunuma, T., Chang, J., & Kondo, A. (2014) · Biotechnology Advances · doi:10.1016/j.biotechadv.2014.09.002 · Cited by 313

Genome editing of model oleaginous microalgae Nannochloropsis spp. by CRISPR /Cas9

Wang, Q., Lu, Y., Xin, Y., Wei, L., Huang, S., & Xu, J. (2016) · The Plant Journal · doi:10.1111/tpj.13307 · Cited by 312

Cpf1 Is a Versatile Tool for CRISPR Genome Editing Across Diverse Species of Cyanobacteria

Ungerer, J., & Pakrasi, H. B. (2016) · Scientific Reports · doi:10.1038/srep39681 · Cited by 308

The Regulatory Status of Genome‐edited Crops

Wolt, J. D., Wang, K., & Yang, B. (2015) · Plant Biotechnology Journal · doi:10.1111/pbi.12444 · Cited by 292

Rapid profiling of a microbial genome using mixtures of barcoded oligonucleotides

Warner, J. R., Reeder, P. J., Karimpour‐Fard, A., Woodruff, L. B. A., & Gill, R. T. (2010) · Nature Biotechnology · doi:10.1038/nbt.1653 · Cited by 284

Treated Wastewater Irrigation—A Review

Hashem, M. S., & Qi, X. (2021) · Water · doi:10.3390/w13111527 · Cited by 262

Third generation biofuels: A nutritional perspective in enhancing microbial lipid production

Leong, W.-H., Lim, J.-W., Lam, M.-K., Uemura, Y., & Ho, Y.-C. (2018) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2018.04.066 · Cited by 259

Development of renewable energy sources market and biofuels in The European Union

Bórawski, P., Bełdycka-Bórawska, A., Szymańska, E. J., Jankowski, K. J., Dubis, B., & Dunn, J. W. (2019) · Journal of Cleaner Production · doi:10.1016/j.jclepro.2019.04.242 · Cited by 254

Application of CRISPRi for prokaryotic metabolic engineering involving multiple genes, a case study: Controllable P(3HB-co-4HB) biosynthesis

Lv, L., Ren, Y., Chen, J., Wu, Q., & Chen, G. (2015) · Metabolic Engineering · doi:10.1016/j.ymben.2015.03.013 · Cited by 252

Multiple Gene Repression in Cyanobacteria Using CRISPRi

Yao, L., Cengic, I., Anfelt, J., & Hudson, E. P. (2016) · ACS Synthetic Biology · doi:10.1021/acssynbio.5b00264 · Cited by 246

CRMAGE: CRISPR Optimized MAGE Recombineering

Ronda, C., Pedersen, L. E., Sommer, M. O. A., & Nielsen, A. T. (2016) · Scientific Reports · doi:10.1038/srep19452 · Cited by 228

Two-stage cultivation strategy for simultaneous increases in growth rate and lipid content of microalgae: A review

Aziz, M. M. A., Kassim, K. A., Shokravi, Z., Jakarni, F. M., Liu, H., Zaini, N., Tan, L. S., Islam, A. B. M. S., & Shokravi, H. (2019) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2019.109621 · Cited by 225

CRISPRi mediated phosphoenolpyruvate carboxylase regulation to enhance the production of lipid in Chlamydomonas reinhardtii

Kao, P., & Ng, I. (2017) · Bioresource Technology · doi:10.1016/j.biortech.2017.04.111 · Cited by 222

Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria

Dong, C., Fontana, J. T., Patel, A., Carothers, J. M., & Zalatan, J. G. (2018) · Nature Communications · doi:10.1038/s41467-018-04901-6 · Cited by 221

Expression of fatty acid synthesis genes and fatty acid accumulation in haematococcus pluvialis under different stressors

Lei, A., Chen, H., Shen, G., Hu, Z., Chen, L., & Wang, J. (2012) · Biotechnology for Biofuels · doi:10.1186/1754-6834-5-18 · Cited by 211

Wastewater treatment by microalgae

Plöhn, M., Spain, O., Sirin, S., Silva, M., Escudero‐Oñate, C., Ferrando‐Climent, L., Allahverdiyeva, Y., & Funk, C. (2021) · Physiologia Plantarum · doi:10.1111/ppl.13427 · Cited by 203

CRISPR interference as a titratable, trans-acting regulatory tool for metabolic engineering in the cyanobacterium Synechococcus sp. strain PCC 7002

Gordon, G. C., Korosh, T. C., Cameron, J. C., Markley, A. L., Begemann, M. B., & Pfleger, B. F. (2016) · Metabolic Engineering · doi:10.1016/j.ymben.2016.07.007 · Cited by 189

Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana

Hopes, A., Nekrasov, V., Kamoun, S., & Möck, T. (2016) · Plant Methods · doi:10.1186/s13007-016-0148-0 · Cited by 182

Enhancement of lipid accumulation in microalgae by metabolic engineering

Sun, X., Ren, L., Zhao, Q., Ji, X., & Huang, H. (2018) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2018.10.004 · Cited by 181

Marine microalgae for production of biofuels and chemicals

Maeda, Y., Yoshino, T., Matsunaga, T., Matsumoto, M., & Tanaka, T. (2018) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2017.11.018 · Cited by 177

Correction: Corrigendum: Minimizing the risk of allo-sensitization to optimize the benefit of allogeneic cardiac-derived stem/progenitor cells

Hocine, H. R., Costa, H. E., Dam, N., Giustiniani, J., Palacios, I., Loiseau, P., Bensussan, A., Borlado, L. R., Charron, D. J., Suberbielle, C., et al. (2017) · Scientific Reports · doi:10.1038/srep44720 · Cited by 175

One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing

Serif, M., Dubois, G., Finoux, A.-L., Teste, M.-A., Jallet, D., & Daboussi, F. (2018) · Nature Communications · doi:10.1038/s41467-018-06378-9 · Cited by 165

CRISPR interference (CRISPRi) for gene regulation and succinate production in cyanobacterium S. elongatus PCC 7942

Huang, C.-H., Shen, C. R., Li, H., Sung, L.-Y., Wu, M.-Y., & Hu, Y.-C. (2016) · Microbial Cell Factories · doi:10.1186/s12934-016-0595-3 · Cited by 164

Multi-functional genome-wide CRISPR system for high throughput genotype–phenotype mapping

Lian, J., Schultz, J. C., Cao, M., HamediRad, M., & Zhao, H. (2019) · Nature Communications · doi:10.1038/s41467-019-13621-4 · Cited by 164

Engineering Escherichia coli for efficient production of 5-aminolevulinic acid from glucose

Kang, Z., Wang, Y., Gu, P., Wang, Q., & Qi, Q. (2011) · Metabolic Engineering · doi:10.1016/j.ymben.2011.05.003 · Cited by 163

Overexpression of acetyl-CoA synthetase increased the biomass and fatty acid proportion in microalga Schizochytrium

Yan, J., Cheng, R., Lin, X., You, S., Li, K., Rong, H., & Ma, Y. (2012) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-012-4481-6 · Cited by 160

Large Scale Cultivation of Genetically Modified Microalgae: A New Era for Environmental Risk Assessment

Beacham, T. A., Sweet, J. B., & Allen, M. J. (2017) · Algal Research · doi:10.1016/j.algal.2017.04.028 · Cited by 160

Revolutions in algal biochar for different applications: State-of-the-art techniques and future scenarios

Chen, Y.-D., Liu, F., Ren, N., & Ho, S. (2020) · Chinese Chemical Letters · doi:10.1016/j.cclet.2020.08.019 · Cited by 160

Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes

Yao, L., Shabestary, K., Björk, S., Asplund‐Samuelsson, J., Joensson, H. N., Jahn, M., & Hudson, E. P. (2020) · Nature Communications · doi:10.1038/s41467-020-15491-7 · Cited by 158

Multilayered genetic safeguards limit growth of microorganisms to defined environments

Gallagher, R. R., Patel, J. R., Interiano, A. L., Rovner, A. J., & Isaacs, F. J. (2015) · Nucleic Acids Research · doi:10.1093/nar/gku1378 · Cited by 156

Overexpression of Malonyl-CoA: ACP Transacylase in Schizochytrium sp. to Improve Polyunsaturated Fatty Acid Production

Li, Z., Meng, T., Ling, X., Li, J., Zheng, C., Shi, Y., Chen, Z., Li, Z., Li, Q., Lu, Y., et al. (2018) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.8b01026 · Cited by 155

Inactivation of P haeodactylum tricornutum urease gene using transcription activator‐like effector nuclease‐based targeted mutagenesis

Weyman, P. D., Beeri, K., Lefebvre, S. C., Rivera, J., McCarthy, J. K., Heuberger, A. L., Peers, G., Allen, A. E., & Dupont, C. L. (2014) · Plant Biotechnology Journal · doi:10.1111/pbi.12254 · Cited by 147

Observation of giant spin split Fermi arc with maximal Chern number in the chiral topological semimetal PtGa

Yao, M., Manna, K., Yang, Q., Fedorov, A., Voroshnin, V. Y., Schwarze, B. V., Hornung, J., Chattopadhyay, S., Sun, Z., Guin, S. N., et al. (2020) · HZB Repository (Helmholtz-Zentrum Berlin für Materialien und Energie GmbH (HZB)) · Cited by 146

Coproduct market analysis and water footprint of simulated commercial algal biorefineries

Subhadra, B. G., & Edwards, M. (2011) · Applied Energy · doi:10.1016/j.apenergy.2010.12.051 · Cited by 141

Transgenic cotton and sterile insect releases synergize eradication of pink bollworm a century after it invaded the United States

Tabashnik, B. E., Liesner, L., Ellsworth, P. C., Unnithan, G. C., Fabrick, J. A., Naranjo, S. E., Li, X., Dennehy, T. J., Antilla, L., Staten, R. T., et al. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2019115118 · Cited by 141

The application of the CRISPR-Cas9 genome editing machinery in food and agricultural science: Current status, future perspectives, and associated challenges

Eş, İ., Gavahian, M., Martí-Quijal, F. J., Lorenzo, J. M., Khaneghah, A. M., Tsatsanis, C., Kampranis, S. C., & Barba, F. J. (2019) · Biotechnology Advances · doi:10.1016/j.biotechadv.2019.02.006 · Cited by 139

Monitoring the biological activity of micropollutants during advanced wastewater treatment with ozonation and activated carbon filtration

Macova, M., Escher, B. I., Reungoat, J., Carswell, S., Chue, K. L., Keller, J., & Mueller, J. F. (2009) · Water Research · doi:10.1016/j.watres.2009.09.025 · Cited by 138

Enhanced lipid productivity in AGP knockout marine microalga Tetraselmis sp. using a DNA-free CRISPR-Cas9 RNP method

Chang, K. S., Kim, J., Park, H., Hong, S.-J., Lee, C., & Jin, E. (2020) · Bioresource Technology · doi:10.1016/j.biortech.2020.122932 · Cited by 137

Synthetic biology: Recent progress, biosafety and biosecurity concerns, and possible solutions

Wang, F., & Zhang, W. (2019) · Journal of Biosafety and Biosecurity · doi:10.1016/j.jobb.2018.12.003 · Cited by 135

Engineered CRISPRa enables programmable eukaryote-like gene activation in bacteria

Liu, Y., Wan, X., & Wang, B. (2019) · Nature Communications · doi:10.1038/s41467-019-11479-0 · Cited by 135

Biomass Productivities in Wild Type and Pigment Mutant of Cyclotella sp. (Diatom)

Huesemann, M. H., Hausmann, T. S., Bartha, R., Aksoy, M., Weissman, J. C., & Benemann, J. R. (2009) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-008-8298-9 · Cited by 133

Transgenic microalgae as a non-antibiotic bactericide producer to defend against bacterial pathogen infection in the fish digestive tract

Li, S.-S., & Tsai, H.-J. (2009) · Fish & Shellfish Immunology · doi:10.1016/j.fsi.2008.07.004 · Cited by 130

Modular Metabolic Engineering for Biobased Chemical Production

Lu, H., Villada, J. C., & Lee, P. K. H. (2018) · Trends in biotechnology · doi:10.1016/j.tibtech.2018.07.003 · Cited by 127

A genetic manipulation system for oceanic cyanobacteria of the genus Synechococcus

Brahamsha, B. (1996) · Applied and Environmental Microbiology · doi:10.1128/aem.62.5.1747-1751.1996 · Cited by 124

Agro-industrial waste to biobutanol production: eco-friendly biofuels for next generation

Huzir, N. M., Aziz, M. M. A., Ismail, S. B., Abdullah, B., Mahmood, N. A. N., Umor, N. A., & Syed Muhammad, S. A. F. (2018) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2018.06.036 · Cited by 121

Improving lipid production by strain development in microalgae: Strategies, challenges and perspectives

Park, S., Nguyen, T., & Jin, E. (2019) · Bioresource Technology · doi:10.1016/j.biortech.2019.121953 · Cited by 121

Diversion of the Long-Chain Acyl-ACP Pool in Synechocystis to Fatty Alcohols through CRISPRi Repression of the Essential Phosphate Acyltransferase PlsX

Kaczmarzyk, D., Cengic, I., Yao, L., & Hudson, E. P. (2018) · Metabolic Engineering · doi:10.1016/j.ymben.2017.11.014 · Cited by 120

The advances and limitations in biodiesel production: feedstocks, oil extraction methods, production, and environmental life cycle assessment

Pikula, K., Zakharenko, A. M., Stratidakis, A. K., Razgonova, M. P., Nosyrev, A. E., Mezhuev, Y. O., Tsatsakis, A. M., & Golokhvast, K. S. (2020) · Green Chemistry Letters and Reviews · doi:10.1080/17518253.2020.1829099 · Cited by 119

Identification of a malonyl CoA‐acyl carrier protein transacylase and its regulatory role in fatty acid biosynthesis in oleaginous microalga Nannochloropsis oceanica

Chen, J., Liu, W., Hu, D., Wang, X., Balamurugan, S., Alimujiang, A., Yang, W., Liu, J., & Li, H. (2017) · Biotechnology and Applied Biochemistry · doi:10.1002/bab.1531 · Cited by 111

Recent insights into cotton functional genomics: progress and future perspectives

Ashraf, J., Zuo, D., Wang, Q., Malik, W., Zhang, Y., Abid, M. A., Cheng, H., Yang, Q., & Song, G. (2017) · Plant Biotechnology Journal · doi:10.1111/pbi.12856 · Cited by 109

Trypanocidal and leishmanicidal activities of different antimicrobial peptides (AMPs) isolated from aquatic animals

Löfgren, S. E., Miletti, L. C., Steindel, M., Evelyne Bachère, B., & Barracco, M. A. (2007) · Experimental Parasitology · doi:10.1016/j.exppara.2007.07.011 · Cited by 108

Conditional Suicide System for Containment of Bacteria and Plasmids

Molin, S., Klemm, P., Poulsen, L. K., Biehl, H., Gerdes, K., & Andersson, P. (1987) · Nature Biotechnology · doi:10.1038/nbt1287-1315 · Cited by 108

Biomass and Biofuels from Microalgae

Moheimani, N. R., McHenry, M. P., de Boer, K., & Bahri, P. A. (2015) · Biofuel and biorefinery technologies · doi:10.1007/978-3-319-16640-7 · Cited by 105

Advances and challenges in genetic engineering of microalgae

Fajardo, C., Donato, M. D., Carrasco-Reinado, R., Martı́nez-Rodrı́guez, G., Mancera, J. M., & Fernández‐Acero, F. J. (2019) · Reviews in Aquaculture · doi:10.1111/raq.12322 · Cited by 105

Ethical implications of the use of whole genome methods in medical research

Kaye, J. S., Boddington, P., de Vries, J., Hawkins, N., & Melham, K. (2009) · European Journal of Human Genetics · doi:10.1038/ejhg.2009.191 · Cited by 103

Improving ‘Lipid Productivity’ in Microalgae by Bilateral Enhancement of Biomass and Lipid Contents: A Review

Shokravi, Z., Shokravi, H., Ong, H. C., Lau, W. J., Koloor, S. S. R., Petrů, M., & Ismail, A. F. (2020) · Sustainability · doi:10.3390/su12219083 · Cited by 103

The commercialization of genome-editing technologies

Brinegar, K. N., Yetisen, A. K., Choi, S. K., Vallillo, E., Ruiz‐Esparza, G. U., Prabhakar, A. M., Khademhosseini, A., & Yun, S. H. (2017) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2016.1271768 · Cited by 102

Development of an antibiotic-free plasmid selection system based on glycine auxotrophy for recombinant protein overproduction in Escherichia coli

Vidal, L., Pinsach, J., Striedner, G., Caminal, G., & Ferrer, P. (2008) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2008.01.011 · Cited by 99

Genome sequence assembly: algorithms and issues

Pop, M., Salzberg, S. L., & Shumway, M. F. (2002) · Computer · doi:10.1109/mc.2002.1016901 · Cited by 97

Whole-Genome Resequencing Reveals Extensive Natural Variation in the Model Green Alga Chlamydomonas reinhardtii

Flowers, J. M., Hazzouri, K. M., Pham, G. M., Rosas, U., Bahmani, T., Khraiwesh, B., Nelson, D. R., Jijakli, K., Abdrabu, R., Harris, E. H., et al. (2015) · The Plant Cell · doi:10.1105/tpc.15.00492 · Cited by 96

Conditional-Suicide Containment System for Bacteria Which Mineralize Aromatics

Contreras, A., Molin, S., & Ramos, J. L. (1991) · Applied and Environmental Microbiology · doi:10.1128/aem.57.5.1504-1508.1991 · Cited by 95

Targeted Repression of Essential Genes To Arrest Growth and Increase Carbon Partitioning and Biofuel Titers in Cyanobacteria

Shabestary, K., Anfelt, J., Ljungqvist, E., Jahn, M., Yao, L., & Hudson, E. P. (2018) · ACS Synthetic Biology · doi:10.1021/acssynbio.8b00056 · Cited by 89

Genetically modified crops, regulatory delays, and international trade

Smyth, S. J. (2017) · Food and Energy Security · doi:10.1002/fes3.100 · Cited by 83

From Current Algae Products to Future Biorefinery Practices: A Review

Eppink, M. H. M., Olivieri, G., Reith, H., van den Berg, C., Barbosa, M. J., & Wijffels, R. H. (2017) · Advances in biochemical engineering, biotechnology · doi:10.1007/10_2016_64 · Cited by 81

Altered lipid accumulation in Nannochloropsis salina CCAP849/3 following EMS and UV induced mutagenesis

Beacham, T. A., Macia, V. M., Rooks, P., White, D. A., & Ali, S. T. (2015) · Biotechnology Reports · doi:10.1016/j.btre.2015.05.007 · Cited by 80

Transgenic Microalgae as Green Cell Factories

Back, N., Cohen, I. R., Fernández, E. M., Galván, A., Lambris, J. D., Paoletti, R., Lajtha, A., & León, R. (2007) · Advances in experimental medicine and biology · doi:10.1007/978-0-387-75532-8 · Cited by 80

Recent advances in synthetic biology of cyanobacteria

Sengupta, A., Pakrasi, H. B., & Wangikar, P. P. (2018) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-018-9046-x · Cited by 78

Widespread Enhancer Activity from Core Promoters

Medina-Rivera, A., Santiago-Algarra, D., Puthier, D., & Spicuglia, S. (2018) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2018.03.004 · Cited by 78

Viral inhibitors derived from macroalgae, microalgae, and cyanobacteria: A review of antiviral potential throughout pathogenesis

Reynolds, D., Huesemann, M. H., Edmundson, S. J., Sims, A. M., Hurst, B. L., Cady, S. L., Beirne, N., Freeman, J., Berger, A., & Gao, S. (2021) · Algal Research · doi:10.1016/j.algal.2021.102331 · Cited by 78

Biofuel and food security in China and Japan

Koizumi, T. (2013) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2012.12.047 · Cited by 76

Regulating Biotech Trade: the Cartegena Protocol on Biosafety

Falkner, R. (2000) · International Affairs · doi:10.1111/1468-2346.00135 · Cited by 76

Breeding of high biomass and lipid producing Desmodesmus sp. by Ethylmethane sulfonate-induced mutation

Zhang, Y., He, M., Zou, S., Fei, C., Yan, Y., Zheng, S., Ahmed, R. A., & Wang, C. (2016) · Bioresource Technology · doi:10.1016/j.biortech.2016.01.120 · Cited by 76

A fast and reliable strategy to generate TALEN-mediated gene knockouts in the diatom Phaeodactylum tricornutum

Serif, M., Bernard, L., Weißert, K., Kroth, P. G., & Bártulos, C. R. (2017) · Algal Research · doi:10.1016/j.algal.2017.02.005 · Cited by 76

A substrate-dependent biological containment system for Pseudomonas putida based on the Escherichia coli gef gene

Jensen, L., Ramos, J. L., Kaneva, Z., & Molin, S. (1993) · Applied and Environmental Microbiology · doi:10.1128/aem.59.11.3713-3717.1993 · Cited by 74

Bacterial Toxin-Antitoxin Gene System as Containment Control in Yeast Cells

Kristoffersen, P., Jensen, G. B., Gerdes, K., & Piškur, J. (2000) · Applied and Environmental Microbiology · doi:10.1128/aem.66.12.5524-5526.2000 · Cited by 74

Genome scale engineering techniques for metabolic engineering

Liu, R., Bassalo, M. C., Zeitoun, R. I., & Gill, R. T. (2015) · Metabolic Engineering · doi:10.1016/j.ymben.2015.09.013 · Cited by 71

A gene-within-a-gene Cas9/sgRNA hybrid construct enables gene editing and gene replacement strategies in Chlamydomonas reinhardtii

Jiang, W. Z., & Weeks, D. P. (2017) · Algal Research · doi:10.1016/j.algal.2017.04.001 · Cited by 71

Algal biofuels in Canada: Status and potential

Scaife, M. A., Merkx-Jacques, A., Woodhall, D. L., & Armenta, R. E. (2015) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2014.12.024 · Cited by 70

Intrinsic biocontainment: Multiplex genome safeguards combine transcriptional and recombinational control of essential yeast genes

Cai, Y., Agmon, N., Choi, W. J., Ubide, A., Stracquadanio, G., Caravelli, K., Hao, H., Bader, J. S., & Boeke, J. D. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1424704112 · Cited by 69

Xenomicrobiology: a roadmap for genetic code engineering

Acevedo‐Rocha, C. G., & Budiša, N. (2016) · Microbial Biotechnology · doi:10.1111/1751-7915.12398 · Cited by 67

Improvement in Oil Production by Increasing Malonyl-CoA and Glycerol-3-Phosphate Pools in Scenedesmus quadricauda

Gomma, A. E., Lee, S., Sun, S. M., Yang, S. H., & Chung, G. (2015) · Indian Journal of Microbiology · doi:10.1007/s12088-015-0546-4 · Cited by 66

CHLOROPLAST GENETIC TOOL FOR THE GREEN MICROALGAE HAEMATOCOCCUS PLUVIALIS (CHLOROPHYCEAE, VOLVOCALES) 1

Gutiérrez, C. L., Gimpel, J. A., Escobar, C., Marshall, S. H., & Henríquez, V. (2012) · Journal of Phycology · doi:10.1111/j.1529-8817.2012.01178.x · Cited by 64

Reduction of UV-B radiation causes an enhancement of photoinhibition in high light stressed aquatic plants from New Zealand lakes

Hanelt, D., Hawes, I., & Rae, R. (2006) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/j.jphotobiol.2006.01.013 · Cited by 62

Sustainable Biofuels Development in India

Sukumaran, R. K., & Chandel, A. K. (2017) · Springer eBooks · doi:10.1007/978-3-319-50219-9 · Cited by 62

Emerging Species and Genome Editing Tools: Future Prospects in Cyanobacterial Synthetic Biology

Gale, G. A. R., Schiavon Osorio, A. A., Mills, L. A., Wang, B., Lea-Smith, D. J., & McCormick, A. J. (2019) · Microorganisms · doi:10.3390/microorganisms7100409 · Cited by 61

Establishment of a Genome Editing Tool Using CRISPR-Cas9 in Chlorella vulgaris UTEX395

Kim, J., Chang, K. S., Lee, S., & Jin, E. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22020480 · Cited by 61

Effect of Croton megalocarpus, Calophyllum inophyllum, Moringa oleifera, palm and coconut biodiesel–diesel blending on their physico-chemical properties

Atabani, A. E., Mofijur, M., Masjuki, H. H., Badruddin, I. A., Kalam, M. A., & Chong, W. T. (2014) · Industrial Crops and Products · doi:10.1016/j.indcrop.2014.06.011 · Cited by 60

A Novel Biocontainment Strategy Makes Bacterial Growth and Survival Dependent on Phosphite

Hirota, R., Abe, K., Katsuura, Z.-I., Noguchi, R., Moribe, S., Motomura, K., Ishida, T., Alexandrov, M., Funabashi, H., Ikeda, T., et al. (2017) · Scientific Reports · doi:10.1038/srep44748 · Cited by 59

Tunable Repression of Key Photosynthetic Processes Using Cas12a CRISPR Interference in the Fast-Growing Cyanobacterium Synechococcus sp. UTEX 2973

Knoot, C. J., Biswas, S., & Pakrasi, H. B. (2019) · ACS Synthetic Biology · doi:10.1021/acssynbio.9b00417 · Cited by 58

Evaluation of central metabolism based on a genomic database ofSynechocystis PCC6803

Hong, S.-J., & Lee, C. (2007) · Biotechnology and Bioprocess Engineering · doi:10.1007/bf03028644 · Cited by 57

Metabolic engineering of Corynebacterium glutamicum by synthetic small regulatory RNAs

Sun, D., Chen, J., Wang, Y., Li, M., Rao, D., Guo, Y., Chen, N., Zheng, P., Sun, J., & Ma, Y. (2019) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-018-02128-4 · Cited by 57

Efficient Editing of the Nuclear APT Reporter Gene in Chlamydomonas reinhardtii via Expression of a CRISPR-Cas9 Module

Guzmán-Zapata, D., Sandoval-Vargas, J. M., Macedo-Osorio, K. S., Salgado, E., Castrejón-Flores, J. L., Oliver-Salvador, M. D. C., Durán‐Figueroa, N., Nogué, F., & Badillo-Corona, J. A. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20051247 · Cited by 57

Synthetic Biology and Biosecurity: Challenging the “Myths”

Jefferson, C., Lentzos, F., & Marris, C. (2014) · Frontiers in Public Health · doi:10.3389/fpubh.2014.00115 · Cited by 56

Engineering the metabolic pathways of lipid biosynthesis to develop robust microalgal strains for biodiesel production

Shahid, A., Rehman, A. U., Usman, M., Ashraf, M. U., Javed, M. R., Khan, A. Z., Gill, S. S., & Mehmood, M. A. (2019) · Biotechnology and Applied Biochemistry · doi:10.1002/bab.1812 · Cited by 56

Global economic, environmental and health benefits from GM crop adoption

Smyth, S. J., Kerr, W. A., & Phillips, P. W. B. (2015) · Global Food Security · doi:10.1016/j.gfs.2015.10.002 · Cited by 55

Environmental Risks of Large Scale Cultivation of Microalgae: Mitigation of Spills

Gressel, J., van der Vlugt, C. J. B., & Bergmans, H. E. N. (2013) · Algal Research · doi:10.1016/j.algal.2013.04.002 · Cited by 55

Construction of a self-cloning system in the unicellular green alga Pseudochoricystis ellipsoidea

Kasai, Y., Oshima, K., Ikeda, F., Abe, J., Yoshimitsu, Y., & Harayama, S. (2015) · Biotechnology for Biofuels · doi:10.1186/s13068-015-0277-0 · Cited by 54

Expanded synthetic small regulatory RNA expression platforms for rapid and multiplex gene expression knockdown

Yang, D., Yoo, S. M., Gu, C., Ryu, J. Y., Lee, J. E., & Lee, S. Y. (2019) · Metabolic Engineering · doi:10.1016/j.ymben.2019.04.003 · Cited by 53

CONSTRUCTION AND USE OF SAFER BACTERIAL HOST STRAINS FOR RECOMBINANT DNA RESEARCH

Curtiss, R., Inoue, M., Pereira, D. A., Hsu, J. C., Alexander, L., & ROCK, L. (1977) · Elsevier eBooks · doi:10.1016/b978-0-12-634250-5.50011-2 · Cited by 51

Handbook on Agriculture, Biotechnology and Development

Aerni, P. (2014) · Edward Elgar Publishing eBooks · doi:10.4337/9780857938350 · Cited by 47

Agrigenomics for Microalgal Biofuel Production: An Overview of Various Bioinformatics Resources and Recent Studies to Link OMICS to Bioenergy and Bioeconomy

Misra, N., Panda, P. K., & Parida, B. K. (2013) · OMICS A Journal of Integrative Biology · doi:10.1089/omi.2013.0025 · Cited by 46

Growth and selection of the cyanobacterium Synechococcus sp. PCC 7002 using alternative nitrogen and phosphorus sources

Selão, T. T., Włodarczyk, A., Nixon, P. J., & Norling, B. (2019) · Metabolic Engineering · doi:10.1016/j.ymben.2019.04.013 · Cited by 46

Genetic Biocontainment Systems for the Safe Use of Engineered Microorganisms

Kim, D., & Lee, J. W. (2020) · Biotechnology and Bioprocess Engineering · doi:10.1007/s12257-020-0070-1 · Cited by 44

Augmented lipid accumulation in ethyl methyl sulphonate mutants of oleaginous microalga for biodiesel production

Mehtani, J., Arora, N., Patel, A., Jain, P., Pruthi, P. A., Poluri, K. M., & Pruthi, V. (2017) · Bioresource Technology · doi:10.1016/j.biortech.2017.03.108 · Cited by 43

Innovations in improving lipid production: Algal chemical genetics

Wase, N. V., Black, P., & Dirusso, C. (2018) · Progress in Lipid Research · doi:10.1016/j.plipres.2018.07.001 · Cited by 43

CRISPR as a strong gene editing tool

Shen, S., Loh, T. J., Shen, H., Zheng, X., & Shen, H. (2017) · BMB Reports · doi:10.5483/bmbrep.2017.50.1.128 · Cited by 42

The next step for the synthetic genome

Baker, M. (2011) · Nature · doi:10.1038/473403a · Cited by 41

Implications of biological information digitization: Access and benefit sharing of plant genetic resources

Smyth, S. J., Macall, D. M., Phillips, P. W. B., & de Beer, J. (2020) · The Journal of World Intellectual Property · doi:10.1111/jwip.12151 · Cited by 41

The history and market impact of CRISPR RNA-guided nucleases

van Erp, P. B. G., Bloomer, G., Wilkinson, R. A., & Wiedenheft, B. (2015) · Current Opinion in Virology · doi:10.1016/j.coviro.2015.03.011 · Cited by 40

Hydrothermal gasification of Acutodesmus obliquus for renewable energy production and nutrient recycling of microalgal mass cultures

Patzelt, D. J., Hindersin, S., Elsayed, S., Boukis, Ν., Kerner, M., & Hanelt, D. (2014) · Journal of Applied Phycology · doi:10.1007/s10811-014-0496-y · Cited by 40

Third Generation Biofuels

Bajpai, P. (2018) · SpringerBriefs in energy · doi:10.1007/978-981-13-2378-2 · Cited by 40

Alternative Biochemistries for Alien Life: Basic Concepts and Requirements for the Design of a Robust Biocontainment System in Genetic Isolation

Diwo, C., & Budiša, N. (2018) · Genes · doi:10.3390/genes10010017 · Cited by 40

Recent advances in genetic engineering tools based on synthetic biology

Ren, J., Lee, J., & Na, D. (2020) · The Journal of Microbiology · doi:10.1007/s12275-020-9334-x · Cited by 39

On the road to synthetic life: the minimal cell and genome-scale engineering

Juhas, M. (2015) · Critical Reviews in Biotechnology · doi:10.3109/07388551.2014.989423 · Cited by 38

Low escape-rate genome safeguards with minimal molecular perturbation of Saccharomyces cerevisiae

Agmon, N., Tang, Z., Yang, K., Sutter, B. A., Ikushima, S., Cai, Y., Caravelli, K., Martin, J. A., Sun, X., Choi, W. J., et al. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1621250114 · Cited by 38

Controlling the Implementation of Transgenic Microbes: Are We Ready for What Synthetic Biology Has to Offer?

Stirling, F., & Silver, P. A. (2020) · Molecular Cell · doi:10.1016/j.molcel.2020.03.034 · Cited by 38

High-CO 2 Requirement as a Mechanism for the Containment of Genetically Modified Cyanobacteria

Clark, R. L., Gordon, G. C., Bennett, N. R., Lyu, H., Root, T. W., & Pfleger, B. F. (2018) · ACS Synthetic Biology · doi:10.1021/acssynbio.7b00377 · Cited by 34

Genome Sequence of the Oleaginous Green Alga, Chlorella vulgaris UTEX 395

Guarnieri, M. T., Levering, J., Henard, C. A., Boore, J. L., Betenbaugh, M. J., Zengler, K., & Knoshaug, E. P. (2018) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2018.00037 · Cited by 34

Genomic Characterization of Cyanophage vB_AphaS-CL131 Infecting Filamentous Diazotrophic Cyanobacterium Aphanizomenon flos-aquae Reveals Novel Insights into Virus-Bacterium Interactions

Šulčius, S., Šimoliūnas, E., Alzbutas, G., Gasiūnas, G., Jauniškis, V., Kuznecova, J., Miettinen, S., Nilsson, E., Meškys, R., Roine, E., et al. (2018) · Applied and Environmental Microbiology · doi:10.1128/aem.01311-18 · Cited by 33

Targeted genome editing in algae using CRISPR/Cas9

Tanwar, A., Sharma, S., & Kumar, S. B. (2018) · Indian Journal of Plant Physiology · doi:10.1007/s40502-018-0423-3 · Cited by 33

In the Grip of Algal Genomics

Grossman, A. R. (2008) · Advances in experimental medicine and biology · doi:10.1007/978-0-387-75532-8_6 · Cited by 32

Economic impacts of commercializing insect-resistant GM maize in China

Xie, W., Ali, T., Cui, Q., & Huang, J. (2017) · China Agricultural Economic Review · doi:10.1108/caer-06-2017-0126 · Cited by 32

Metabolic Engineering for Advanced Biofuels Production and Recent Advances Toward Commercialization

Meadows, C. W., Kang, A., & Lee, T. S. (2017) · Biotechnology Journal · doi:10.1002/biot.201600433 · Cited by 32

Detecting the frequency of aminoglycoside modifying enzyme encoding genes among clinical isolates of methicillin-resistant Staphylococcus aureus

Shokravi, Z., Mehrad, L., & Ramazani, A. (2015) · Bioimpacts · doi:10.15171/bi.2015.15 · Cited by 31

CRISPR: A new paradigm of theranostics

Yadav, N., Narang, J., Chhillar, A. K., & Rana, J. S. (2021) · Nanomedicine Nanotechnology Biology and Medicine · doi:10.1016/j.nano.2020.102350 · Cited by 31

Engineering genomes in multiplex

Woodruff, L. B. A., & Gill, R. T. (2011) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2011.04.019 · Cited by 30

Efficacy of aLactococcus lactisΔpyrGvaccine delivery platform expressing chromosomally integratedhlyfromListeria monocytogenes

Bahey-El-Din, M., Casey, P. G., Griffin, B. T., & Gahan, C. G. M. (2010) · Bioengineered Bugs · doi:10.4161/bbug.1.1.10284 · Cited by 30

The Fourth-Generation Biofuel: A Systematic Review on Nearly Two Decades of Research from 2008 to 2019

Shokravi, Z., Shokravi, H., Aziz, M. A., & Shokravi, H. (2019) · CRC Press eBooks · doi:10.1201/9780429327773-12 · Cited by 30

A conditional suicide system forSaccharomyces cerevisiae relying on the intracellular production of theSerratia marcescens nuclease

Balan, A., & Schenberg, A. C. G. (2005) · Yeast · doi:10.1002/yea.1203 · Cited by 28

Tipping Points in Seaweed Genetic Engineering: Scaling Up Opportunities in the Next Decade

Lin, H., & Qin, S. (2014) · Marine Drugs · doi:10.3390/md12053025 · Cited by 28

Water Footprint of Biodiesel Production from Microalgae Cultivated in Photobioreactors

Feng, P., Zhu, L., Qin, X., & Li, Z. (2016) · Journal of Environmental Engineering · doi:10.1061/(asce)ee.1943-7870.0001150 · Cited by 28

Environmental Issues Surrounding Human Overpopulation

Singh, R. P., Singh, A., & Srivastava, V. (2016) · Advances in environmental engineering and green technologies book series · doi:10.4018/978-1-5225-1683-5 · Cited by 28

Antenna size reduction and altered division cycles in self-cloned, marker-free genetically modified strains of Haematococcus pluvialis

Sharon-Gojman, R., Leu, S., & Zarka, A. (2017) · Algal Research · doi:10.1016/j.algal.2017.09.015 · Cited by 28

Isolation and Characterization of a Novel Bacterium from the Marine Environment for Trichloroacetic Acid Bioremediation

Heidarrezaei, M., Shokravi, H., Huyop, F., Koloor, S. S. R., & Petrů, M. (2020) · Applied Sciences · doi:10.3390/app10134593 · Cited by 27

Complete Genome Sequence of the Model Oleaginous Alga Nannochloropsis gaditana CCMP1894

Schwartz, A. S., Brown, R. C., Ajjawi, I., McCarren, J., Atilla, S., Bauman, N., & Richardson, T. H. (2018) · Genome Announcements · doi:10.1128/genomea.01448-17 · Cited by 26

Genome sequencing, assembly, and annotation of the self-flocculating microalga Scenedesmus obliquus AS-6-11

Chen, B.-L., Mhuantong, W., Ho, S., Chang, J., Zhao, X., & Feng-Wu, B. (2020) · BMC Genomics · doi:10.1186/s12864-020-07142-4 · Cited by 24

Cyanobacterial toxin biosensors for environmental monitoring and protection

Bertani, P., & Lu, W. (2021) · Medicine in Novel Technology and Devices · doi:10.1016/j.medntd.2021.100059 · Cited by 24

Pathways to Obtain Regulatory Approvals for the Use of Genetically Modified Algae in Biofuel or Biobased Chemical Production

Glass, D. J. (2015) · Industrial Biotechnology · doi:10.1089/ind.2015.1503 · Cited by 23

The Ssl2245-Sll1130 Toxin-Antitoxin System Mediates Heat-induced Programmed Cell Death in Synechocystis sp. PCC6803

Srikumar, A., Krishna, P. S., Sivaramakrishna, D., Kopfmann, S., Hess, W. R., Swamy, M. J., Lin‐Chao, S., & Prakash, J. S. S. (2017) · Journal of Biological Chemistry · doi:10.1074/jbc.m116.748178 · Cited by 23

Development of a New Biocontainment Strategy in Model Cyanobacterium Synechococcus Strains

Zhou, Y., Sun, T., Chen, Z., Song, X., Chen, L., & Zhang, W. (2019) · ACS Synthetic Biology · doi:10.1021/acssynbio.9b00282 · Cited by 23

Brucella abortus Strain RB51 Leucine Auxotroph as an Environmentally Safe Vaccine for Plasmid Maintenance and Antigen Overexpression

Rajasekaran, P., Seleem, M. N., Contreras, A., Purwantini, E., Schurig, G. G., Sriranganathan, N., & Boyle, S. M. (2008) · Applied and Environmental Microbiology · doi:10.1128/aem.01511-08 · Cited by 22

Dynamic role and importance of surrogate species for assessing potential adverse environmental impacts of genetically engineered insect-resistant plants on non-target organisms

Wach, M., Hellmich, R. L., Layton, R., Romeis, J., & Gadaleta, P. G. (2016) · Transgenic Research · doi:10.1007/s11248-016-9945-5 · Cited by 22

The morality and ethics governing CRISPR–Cas9 patents in China

Peng, Y. (2016) · Nature Biotechnology · doi:10.1038/nbt.3590 · Cited by 22

Aviation Biofuels: Conversion Routes and Challenges

Chong, J. W., Chemmangattuvalappil, N. G., & Thangalazhy‐Gopakumar, S. (2022) · Springer eBooks · doi:10.1007/978-981-19-4847-3_2 · Cited by 20

Directive 2001 /18/EC on the Deliberate Release into the Environment of GMOs: an Overview and the Main Provisions for Placing on the Market

Garcia, P. R. (2006) · Journal for European Environmental & Planning Law · doi:10.1163/187601006x00029 · Cited by 19

Whole-Genome Shotgun Sequence of Arthrospira platensis Strain Paraca, a Cultivated and Edible Cyanobacterium

Lefort, F., Calmin, G., Crovadore, J., Falquet, J., Hurni, J.-P., Østerås, M., Haldemann, F., & Farinelli, L. (2014) · Genome Announcements · doi:10.1128/genomea.00751-14 · Cited by 17

Fuel Potential of Third Generation Biofuels

Bajpai, P. (2018) · SpringerBriefs in energy · doi:10.1007/978-981-13-2378-2_2 · Cited by 17

Legal But Costly: An Analysis of the EU GM Regulation in the Light of the WTO Trade Dispute Between the EU and the USA

Punt, M., & Wesseler, J. (2015) · World Economy · doi:10.1111/twec.12353 · Cited by 16

Technological and Commercial Update for First- and Second-Generation Ethanol Production in India

Borse, P., & Sheth, A. (2017) · Springer eBooks · doi:10.1007/978-3-319-50219-9_13 · Cited by 16

Spatiotemporal Gene Repression System in the Heterocyst-Forming Multicellular Cyanobacterium Anabaena sp. PCC 7120

Higo, A., & Ehira, S. (2019) · ACS Synthetic Biology · doi:10.1021/acssynbio.8b00496 · Cited by 16

Tools and systems for evolutionary engineering of biomolecules and microorganisms

Jang, S., Kim, M., Hwang, J., & Jung, G. Y. (2019) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-019-02191-5 · Cited by 16

A metric space for semantic containment: Towards the implementation of genetic firewalls

Schmidt, M. R. (2019) · Biosystems · doi:10.1016/j.biosystems.2019.104015 · Cited by 16

Introduction to Bioinformatics in Microbiology

(2018) · Learning materials in biosciences · doi:10.1007/978-3-319-99280-8 · Cited by 16

For the Approval Process of GMOs : The Japanese Case

Ebata, A., Punt, M., & Wesseler, J. (2013) · Cited by 15

In vivo Protein Evolution, Next Generation Protein Engineering Strategy: from Random Approach to Target-specific Approach

Kim, J. Y., Yoo, H., Lee, P., Lee, S., Seo, J., & Kim, B. (2019) · Biotechnology and Bioprocess Engineering · doi:10.1007/s12257-018-0394-2 · Cited by 15

Conflicting Rules for the International Trade of GM Products: Does International Law Provide a Solution?

Kerr, W. A., Smyth, S. J., Phillips, P. W. B., & Phillipson, M. (2014) · MOspace Institutional Repository (University of Missouri) · Cited by 13

Whole-Genome Characterization of Shewanella Algae Strain SYT3 Isolated from Seawater Reveals Insight into Hemolysis

Wu, Z., Ho, S.-P., Cheng, J., Tung, K., Hong, Y., Chen, S., Menzer, A., Chen, Y.-J., Huang, Y., & Liu, P. (2018) · Future Microbiology · doi:10.2217/fmb-2018-0267 · Cited by 13

Gene technology regulation in Australia

Tribe, D. E. (2012) · GM crops & food · doi:10.4161/gmcr.18606 · Cited by 12

Biomass Energy and Biofuels: Perspective, Potentials, and Challenges in the Energy Transition

Mignogna, D., Szabó, M., Ceci, P., & Avino, P. (2024) · Sustainability · doi:10.3390/su16167036 · Cited by 248

New trends on crude glycerol purification: A review

Attarbachi, T., Kingsley, M., & Spallina, V. (2023) · Fuel · doi:10.1016/j.fuel.2023.127485 · Cited by 225

The potential of biofuels from first to fourth generation

Cavelius, P., Engelhart-Straub, S., Mehlmer, N., Lercher, J., Awad, D., & Brück, T. (2023) · PLOS Biology · doi:10.1371/journal.pbio.3002063 · Cited by 224

Recent Advances in the Use of Ionic Liquids and Deep Eutectic Solvents for Lignocellulosic Biorefineries and Biobased Chemical and Material Production

Verdía, P., Choudhary, H., Nakasu, P. Y. S., Ghatta, A. A., Han, Y., Hopson, C., Aravena, R. I., Mishra, D. K., Ovejero-Pérez, A., Simmons, B. A., et al. (2025) · Chemical Reviews · doi:10.1021/acs.chemrev.4c00754 · Cited by 210

A Comprehensive Review of the Properties, Performance, Combustion, and Emissions of the Diesel Engine Fueled with Different Generations of Biodiesel

Zhang, Y., Zhong, Y., Lu, S., Zhang, Z., & Tan, D. (2022) · Processes · doi:10.3390/pr10061178 · Cited by 185

Fourth generation biofuel: A review on risks and mitigation strategies.

Abdullah, B., Syed Muhammad, S. A. F., Shokravi, Z., Ismail, S., Kassim, K. A., Mahmood, A. N., & Aziz, M. M. A. (2019) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2019.02.018 · Cited by 531

Review and Outlook of Fourth-Generation Biofuels: Genetically Engineered Microalgae at the Nexus of Technology, Sustainability, and Policy Challenges

Thakur, R. K., & Sharma, P. (2026) · Energy & Fuels · doi:10.1021/acs.energyfuels.6c01420 · Cited by 1

The Fourth-Generation Biofuel: A Systematic Review on Nearly Two Decades of Research from 2008 to 2019

Shokravi, Z., Shokravi, H., Aziz, M. A., & Shokravi, H. (2019) · CRC Press eBooks · doi:10.1201/9780429327773-12 · Cited by 30

Fourth generation biofuel from genetically modified algal biomass for bioeconomic development

Shokravi, H., Heidarrezaei, M., Shokravi, Z., Ong, H. C., Lau, W. J., Din, M. F. M., & Ismail, A. F. (2022) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2022.10.010 · Cited by 49

PROMISING EVOLUTION OF FOURTH GENERATION BIOFUELS

Malakar, B. (2023) · Iterative International Publishers eBooks · doi:10.58532/v3bars2p1ch14