Multiple Gene Repression in Cyanobacteria Using CRISPRi

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116 · see citing works

Development and Applications of CRISPR-Cas9 for Genome Engineering

Hsu, P. D., Lander, E. S., & Zhang, F. (2014) · Cell · doi:10.1016/j.cell.2014.05.010 · Cited by 5,762 · see citing works

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

Qi, L. S., Larson, M. H., Gilbert, L. A., Doudna, J. A., Weissman, J. S., Arkin, A. P., & Lim, W. A. (2013) · Cell · doi:10.1016/j.cell.2013.02.022 · Cited by 5,016 · see citing works

CRISPR interference (CRISPRi) for sequence-specific control of gene expression

Larson, M. H., Gilbert, L. A., Wang, X., Lim, W. A., Weissman, J. S., & Qi, L. S. (2013) · Nature Protocols · doi:10.1038/nprot.2013.132 · Cited by 1,193 · see citing works

Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system

Bikard, D., Jiang, W., Samai, P., Hochschild, A., Zhang, F., & Marraffini, L. A. (2013) · Nucleic Acids Research · doi:10.1093/nar/gkt520 · Cited by 1,111 · see citing works

Microbial Biosynthesis of Alkanes

Schirmer, A., Rude, M. A., Li, X., Popova, E., & del Cardayre, S. B. (2010) · Science · doi:10.1126/science.1187936 · Cited by 1,040 · see citing works

Engineering complex synthetic transcriptional programs with CRISPR RNA scaffolds

Zalatan, J. G., Lee, M. E., Almeida, R., Gilbert, L. A., Whitehead, E. H., La Russa, M., Tsai, J. C., Weissman, J. S., Dueber, J. E., Qi, L. S., et al. (2015) · Cell · doi:10.1016/j.cell.2014.11.052 · Cited by 938 · see citing works

Photosynthetic production of ethanol from carbon dioxide in genetically engineered cyanobacteria

Gao, Z., Zhao, H. F., Li, Z., Tan, X., & Lü, X. (2012) · Energy & Environmental Science · doi:10.1039/c2ee22675h · Cited by 377 · see citing works

CasOT: A genome-wide cas9/gRNA off-target searching tool

Xiao, A., Cheng, Z., Kong, L., Zhu, Z., Lin, S., Gao, G., & Zhang, B. (2014) · Bioinformatics · doi:10.1093/bioinformatics/btt764 · Cited by 363 · see citing works

An experimentally anchored map of transcriptional start sites in the model cyanobacterium Synechocystis sp. PCC6803

Mitschke, J., Georg, J., Scholz, I., Sharma, C. M., Dienst, D. D., Bantscheff, J., Voß, B., Steglich, C., Wilde, A., Vogel, J., et al. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1015154108 · Cited by 361 · see citing works

Impaired glycogen synthesis causes metabolic overflow reactions and affects stress responses in the cyanobacterium Synechocystis sp. PCC 6803

Gründel, M., Scheunemann, R., Lockau, W., & Zilliges, Y. (2012) · Microbiology · doi:10.1099/mic.0.062950-0 · Cited by 282 · see citing works

Ploidy in cyanobacteria

Griese, M., Lange, C., & Soppa, J. (2011) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2011.02368.x · Cited by 264 · see citing works

Application of CRISPRi for prokaryotic metabolic engineering involving multiple genes, a case study: Controllable P(3HB-co-4HB) biosynthesis

Lv, L., Ren, Y., Chen, J., Wu, Q., & Chen, G. (2015) · Metabolic Engineering · doi:10.1016/j.ymben.2015.03.013 · Cited by 248 · see citing works

CRISPR–Cas system enables fast and simple genome editing of industrial Saccharomyces cerevisiae strains

Šťovíček, V., Borodina, I., & Förster, J. (2015) · Metabolic Engineering Communications · doi:10.1016/j.meteno.2015.03.001 · Cited by 191 · see citing works

Wide-dynamic-range promoters engineered for cyanobacteria

Huang, H.-H., & Lindblad, P. C. (2013) · Journal of Biological Engineering · doi:10.1186/1754-1611-7-10 · Cited by 172 · see citing works

Nitrogen Starvation-Induced Chlorosis inSynechococcus PCC 7942. Low-Level Photosynthesis As a Mechanism of Long-Term Survival

Sauer, J., Schreiber, U., Schmid, R., Völker, U., & Forchhammer, K. (2001) · PLANT PHYSIOLOGY · doi:10.1104/pp.126.1.233 · Cited by 172 · see citing works

Engineering a synthetic pathway in cyanobacteria for isopropanol production directly from carbon dioxide and light

Kusakabe, T., Tatsuke, T., Tsuruno, K., Hirokawa, Y., Atsumi, S., Liao, J. C., & Hanai, T. (2013) · Metabolic Engineering · doi:10.1016/j.ymben.2013.09.007 · Cited by 149 · see citing works

Toward aldehyde and alkane production by removing aldehyde reductase activity in Escherichia coli

Rodriguez, G. M., & Atsumi, S. (2014) · Metabolic Engineering · doi:10.1016/j.ymben.2014.07.012 · Cited by 144 · see citing works

Synechocystis sp. PCC6803 possesses a two-component polyhydroxyalkanoic acid synthase similar to that of anoxygenic purple sulfur bacteria

Hein, S., Tran, H., & Steinbüchel, A. (1998) · Archives of Microbiology · doi:10.1007/s002030050629 · Cited by 134 · see citing works

Metabolic design for cyanobacterial chemical synthesis

Oliver, J. W. K., & Atsumi, S. (2014) · Photosynthesis Research · doi:10.1007/s11120-014-9997-4 · Cited by 134 · see citing works

CRISPR-Cas Systems in the Cyanobacterium Synechocystis sp. PCC6803 Exhibit Distinct Processing Pathways Involving at Least Two Cas6 and a Cmr2 Protein

Scholz, I., Lange, S. J., Hein, S., Hess, W. R., & Backofen, R. (2013) · PLoS ONE · doi:10.1371/journal.pone.0056470 · Cited by 128 · see citing works

Requirement of the Nitrogen Starvation-Induced Protein Sll0783 for Polyhydroxybutyrate Accumulation in Synechocystis sp. Strain PCC 6803

Schlebusch, M., & Forchhammer, K. (2010) · Applied and Environmental Microbiology · doi:10.1128/aem.00484-10 · Cited by 121 · see citing works

Genetic and nutrient modulation of acetyl-CoA levels in Synechocystis for n-butanol production

Anfelt, J., Kaczmarzyk, D., Shabestary, K., Renberg, B., Rockberg, J., Nielsen, J., Uhlén, M., & Hudson, E. P. (2015) · Microbial Cell Factories · doi:10.1186/s12934-015-0355-9 · Cited by 113 · see citing works

Histidine kinases play important roles in the perception and signal transduction of hydrogen peroxide in the cyanobacterium, Synechocystis sp. PCC 6803

Kanesaki, Y., Yamamoto, H., Paithoonrangsarid, K., Shumskaya, M. A., Suzuki, I., Hayashi, H., & Murata, N. (2006) · The Plant Journal · doi:10.1111/j.1365-313x.2006.02959.x · Cited by 111 · see citing works

Using Transcriptomics To Improve Butanol Tolerance of Synechocystis sp. Strain PCC 6803

Anfelt, J., Hallström, B. M., Nielsen, J., Uhlén, M., & Hudson, E. P. (2013) · Applied and Environmental Microbiology · doi:10.1128/aem.02694-13 · Cited by 95 · see citing works

High-Throughput Screen for Poly-3-Hydroxybutyrate in Escherichia coli and Synechocystis sp. Strain PCC6803

Tyo, K. E. J., Zhou, H., & Stephanopoulos, G. N. (2006) · Applied and Environmental Microbiology · doi:10.1128/aem.72.5.3412-3417.2006 · Cited by 91 · see citing works

Adaptation and modification of three CRISPR loci in two closely related cyanobacteria

Hein, S., Scholz, I., Voß, B., & Hess, W. R. (2013) · RNA Biology · doi:10.4161/rna.24160 · Cited by 91 · see citing works

Construction of new synthetic biology tools for the control of gene expression in the cyanobacterium Synechococcus sp. strain PCC 7002

Zess, E. K., Begemann, M. B., & Pfleger, B. F. (2015) · Biotechnology and Bioengineering · doi:10.1002/bit.25713 · Cited by 79 · see citing works

An Organic Acid Based Counter Selection System for Cyanobacteria

Begemann, M. B., Zess, E. K., Walters, E. M., Schmitt, E. F., Markley, A. L., & Pfleger, B. F. (2013) · PLoS ONE · doi:10.1371/journal.pone.0076594 · Cited by 72 · see citing works

A state of the art of metabolic networks of unicellular microalgae and cyanobacteria for biofuel production

Baroukh, C., Muñoz‐Tamayo, R., Steyer, J., & Olivier Bernard, A. (2015) · Metabolic Engineering · doi:10.1016/j.ymben.2015.03.019 · Cited by 71 · see citing works

Characterization of an Alcohol Dehydrogenase from the Cyanobacterium Synechocystis sp. Strain PCC 6803 That Responds to Environmental Stress Conditions via the Hik34-Rre1 Two-Component System

Vidal, R., López‐Maury, L., Guerrero, M. G., & Florencio, F. J. (2009) · Journal of Bacteriology · doi:10.1128/jb.00183-09 · Cited by 67 · see citing works

Genome Engineering in Cyanobacteria: Where We Are and Where We Need To Go

Ramey, C. J., Barón‐Sola, Á., Aucoin, H. R., & Boyle, N. R. (2015) · ACS Synthetic Biology · doi:10.1021/acssynbio.5b00043 · Cited by 65 · see citing works

A novel counter‐selection method for markerless genetic modification in Synechocystis sp. PCC 6803

Cheah, Y. E., Albers, S. C., & Peebles, C. A. M. (2012) · Biotechnology Progress · doi:10.1002/btpr.1661 · Cited by 59 · see citing works

Single-cell screening of photosynthetic growth and lactate production by cyanobacteria

Hammar, P., Angermayr, S. A., Sjostrom, S. L., van der Meer, J., Hellingwerf, K. J., Hudson, E. P., & Joensson, H. N. (2015) · Biotechnology for Biofuels · doi:10.1186/s13068-015-0380-2 · Cited by 50 · see citing works

An AU-box motif upstream of the SD sequence of light-dependent psbA transcripts confers mRNA instability in darkness in cyanobacteria

Agrawal, G. K. (2001) · Nucleic Acids Research · doi:10.1093/nar/29.9.1835 · Cited by 42 · see citing works

The ORF slr0091 of S ynechocystis sp. PCC 6803 encodes a high‐light induced aldehyde dehydrogenase converting apocarotenals and alkanals

Trautmann, D., Beyer, P., & Al‐Babili, S. (2013) · FEBS Journal · doi:10.1111/febs.12361 · Cited by 38 · see citing works

α-Tocopherol Is Essential for Acquired Chill-Light Tolerance in the Cyanobacterium Synechocystis sp. Strain PCC 6803

Yang, Y., Yin, C., Li, W., & Xu, X. (2007) · Journal of Bacteriology · doi:10.1128/jb.01577-07 · Cited by 37 · see citing works

Reduction of lipid peroxidation products and advanced glycation end‐product precursors by cyanobacterial aldo‐keto reductase AKR3G1—a founding member of the AKR3G subfamily

Hintzpeter, J., Martin, H., & Maser, E. (2014) · The FASEB Journal · doi:10.1096/fj.14-258327 · Cited by 10 · see citing works

Biomass and lipid induction strategies in microalgae for biofuel production and other applications

Alishah Aratboni, H., Rafiei, N., Garcia-Granados, R., Alemzadeh, A., & Morones-Ramírez, J. R. (2019) · Microbial Cell Factories · doi:10.1186/s12934-019-1228-4 · Cited by 437 · see citing works

Emerging Technologies in Algal Biotechnology: Toward the Establishment of a Sustainable, Algae-Based Bioeconomy

Fabris, M., Abbriano, R. M., Pernice, M., Sutherland, D. L., Commault, A. S., Hall, C. C., Labeeuw, L., McCauley, J. I., Kuzhiuparambil, U., Ray, P., et al. (2020) · Frontiers in Plant Science · doi:10.3389/fpls.2020.00279 · Cited by 382 · see citing works

CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973

Wendt, K. E., Ungerer, J., Cobb, R. E., Zhao, H., & Pakrasi, H. B. (2016) · Microbial Cell Factories · doi:10.1186/s12934-016-0514-7 · Cited by 232 · see citing works

Genetic engineering of microalgae for enhanced biorefinery capabilities

Fayyaz, M., Chew, K. W., Show, P. L., Ling, T. C., Ng, I.-S., & Chang, J.-S. (2020) · Biotechnology Advances · doi:10.1016/j.biotechadv.2020.107554 · Cited by 225 · see citing works

Cyanogate: a modular cloning suite for engineering cyanobacteria based on the plant moclo syntax

Vasudevan, R., Gale, G. A. R., Schiavon, A. A., Puzorjov, A., Malin, J., Gillespie, M. D., Vavitsas, K., Zulkower, V., Wang, B., Howe, C. J., et al. (2019) · Plant Physiology · doi:10.1104/pp.18.01401 · Cited by 189 · see citing works

CRISPR interference (CRISPRi) for gene regulation and succinate production in cyanobacterium S. elongatus PCC 7942

Huang, C.-H., Shen, C. R., Li, H., Sung, L.-Y., Wu, M.-Y., & Hu, Y.-C. (2016) · Microbial Cell Factories · doi:10.1186/s12934-016-0595-3 · Cited by 164 · see citing works

Biotechnological applications of microalgal oleaginous compounds: current trends on microalgal bioprocessing of products

Mutanda, T., Naidoo, D., Bwapwa, J. K., & Anandraj, A. (2020) · Frontiers in Energy Research · doi:10.3389/fenrg.2020.598803 · Cited by 160 · see citing works

A Critical Review of Genome Editing and Synthetic Biology Applications in Metabolic Engineering of Microalgae and Cyanobacteria

Ng, I., Keskin, B. B., & Tan, S. (2020) · Biotechnology Journal · doi:10.1002/biot.201900228 · Cited by 135 · see citing works

Fourth generation biofuel from genetically modified algal biomass: Challenges and future directions.

Shokravi, H., Shokravi, Z., Heidarrezaei, M., Ong, H. C., Rahimian Koloor, S. S., Petrů, M., Lau, W. J., & Ismail, A. F. (2021) · Chemosphere · doi:10.1016/j.chemosphere.2021.131535 · Cited by 127 · see citing works

Toolboxes for cyanobacteria: recent advances and future direction

Sun, T., Li, S., Song, X., Diao, J., Chen, L., & Zhang, W. (2018) · Biotechnology Advances · doi:10.1016/j.biotechadv.2018.04.007 · Cited by 116 · see citing works

Diversion of the Long-Chain Acyl-ACP Pool in Synechocystis to Fatty Alcohols through CRISPRi Repression of the Essential Phosphate Acyltransferase PlsX

Kaczmarzyk, D., Cengic, I., Yao, L., & Hudson, E. P. (2018) · Metabolic Engineering · doi:10.1016/j.ymben.2017.11.014 · Cited by 120 · see citing works

Recent advances in synthetic biology of cyanobacteria

Sengupta, A., Pakrasi, H. B., & Wangikar, P. P. (2018) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-018-9046-x · Cited by 78 · see citing works

Development of SyneBrick Vectors As a Synthetic Biology Platform for Gene Expression in Synechococcus elongatus PCC 7942

Kim, W. J., Lee, S.-M., Um, Y., Sim, S. J., & Woo, H. M. (2017) · Frontiers in Plant Science · doi:10.3389/fpls.2017.00293 · Cited by 70 · see citing works

Comparative Dose–Response Analysis of Inducible Promoters in Cyanobacteria

Behle, A., Saake, P., Germann, A. T., Dienst, D., & Axmann, I. M. (2020) · ACS Synthetic Biology · doi:10.1021/acssynbio.9b00505 · Cited by 67 · see citing works

Emerging Species and Genome Editing Tools: Future Prospects in Cyanobacterial Synthetic Biology

Gale, G. A. R., Schiavon Osorio, A. A., Mills, L. A., Wang, B., Lea-Smith, D. J., & McCormick, A. J. (2019) · Microorganisms · doi:10.3390/microorganisms7100409 · Cited by 61 · see citing works

Regulatory systems for gene expression control in cyanobacteria

Till, P., Toepel, J., Bühler, B., Mach, R. L., & Mach-Aigner, A. R. (2020) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-019-10344-w · Cited by 54 · see citing works

Recent Progress and Challenges in CRISPR-Cas9 Engineered Algae and Cyanobacteria

Patel, V. K., Das, A., Kumari, R., & Kajla, S. (2023) · Algal Research · doi:10.1016/j.algal.2023.103068 · Cited by 50 · see citing works

Recent advances in synthetic biology of cyanobacteria for improved chemicals production

Wang, F., Gao, Y., & Yang, G. (2020) · Bioengineered · doi:10.1080/21655979.2020.1837458 · Cited by 49 · see citing works

Current understanding of the cyanobacterial CRISPR-Cas systems and development of the synthetic CRISPR-Cas systems for cyanobacteria

Pattharaprachayakul, N., Lee, M., Incharoensakdi, A., & Woo, H. M. (2020) · Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2020.109619 · Cited by 43 · see citing works

Base editing for reprogramming cyanobacterium Synechococcus elongatus

Wang, S.-Y., Li, X., Wang, S.-G., & Xia, P.-F. (2023) · Metabolic Engineering · doi:10.1016/j.ymben.2022.11.005 · Cited by 31 · see citing works

Perspectives of cyanobacterial cell factories

Melis, A., Hidalgo Martinez, D. A., & Betterle, N. (2024) · Photosynthesis Research · doi:10.1007/s11120-023-01056-4 · Cited by 31 · see citing works

Synthetic biology in marine cyanobacteria: Advances and challenges

Bourgade, B., & Stensjö, K. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.994365 · Cited by 28 · see citing works

Customized molecular tools to strengthen metabolic engineering of cyanobacteria

Klähn, S., Opel, F., & Hess, W. R. (2024) · Green Carbon · doi:10.1016/j.greenca.2024.05.002 · Cited by 27 · see citing works

Orthogonal Degron System for Controlled Protein Degradation in Cyanobacteria

Sakkos, J. K., Hernandez-Ortiz, S., Osteryoung, K. W., & Ducat, D. C. (2021) · ACS Synthetic Biology · doi:10.1021/acssynbio.1c00035 · Cited by 21 · see citing works

A single plasmid based CRISPR interference in Synechocystis 6803 – A proof of concept

Kirtania, P., Hódi, B., Mallick, I., Vass, I. Z., Fehér, T., Vass, I., & Kós, P. B. (2019) · PLOS ONE · doi:10.1371/journal.pone.0225375 · Cited by 22 · see citing works

Expressing 2-keto acid pathway enzymes significantly increases photosynthetic isobutanol production

Xie, H., & Lindblad, P. (2022) · Microbial Cell Factories · doi:10.1186/s12934-022-01738-z · Cited by 17 · see citing works

CRISPR-Cas mediated genome engineering of cyanobacteria

Sengupta, A., Liu, D., & Pakrasi, H. B. (2022) · Methods in Enzymology · doi:10.1016/bs.mie.2022.07.023 · Cited by 13 · see citing works

Current advances in CRISPR-Cas-mediated gene editing and regulation in cyanobacteria

Dong, Z., Chen, L., Wang, Y., Sun, T., & Zhang, W. (2024) · Blue Biotechnology · doi:10.1186/s44315-024-00009-3 · Cited by 16 · see citing works

The Molecular Toolset and Techniques Required to Build Cyanobacterial Cell Factories

Opel, F., Axmann, I. M., & Klähn, S. (2022) · Advances in Biochemical Engineering/Biotechnology · doi:10.1007/10_2022_210 · Cited by 7 · see citing works

Advances and Future Perspectives of <i>Synechocystis</i> sp. as a Microbial Cell Factory for Biomanufacturing

He, H., Gao, K., Mao, B., Shi, G., Li, Y., & Deng, X. (2025) · Biotechnology Journal · doi:10.1002/biot.70162 · Cited by 4 · see citing works

Repurposing endogenous Type I-D CRISPR-Cas system for genome editing in Synechococcus sp. PCC7002

Yang, S., Zhang, Y., Li, C., & Tan, X. (2024) · Microbiological Research · doi:10.1016/j.micres.2024.127884 · Cited by 3 · see citing works

Cyanobacterial type I CRISPR-Cas systems: distribution, mechanisms, and genome editing applications

Zhang, Y., Yang, S., Zheng, X., & Tan, X. (2025) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2025.1552030 · Cited by 3 · see citing works

Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression

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ИСПОЛЬЗОВAНИЕ ПОТЕНЦИAЛA СОЦИAЛЬНЫХ ПAРТНЕРОВ В ПОДГОТОВКЕ БУДУЩИХ ПЕДAГОГОВ

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Microfluidic device to suppress the division of large numbers of cyanobacteria

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Molecular Basis of Transient Repression of β-Galactosidase in Escherichia coli

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Microfluidic device for culturing cyanobacteria under the suppression of their division

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Silicification of CyanobacteriaPreprint

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