Antinociceptive effect of cannabinoid agonist WIN 55,212-2 in rats with a spinal cord injury
Open accessAbstract
Spinal cord injury (SCI) pain exhibits many symptoms associated with peripheral neuropathic pain, including increased tactile hypersensitivity. One novel approach to ameliorate SCI pain is the use of cannabinoid (CB) ligands. The current study evaluated the efficacy of the nonselective CB receptor agonist WIN 55,212-2 on tactile hypersensitivity in rats following a brief compression to the thoracic spinal cord. The withdrawal thresholds of the hind paws following SCI were significantly decreased, indicating tactile hypersensitivity. Systemic injection of WIN 55,212-2 increased withdrawal thresholds in a dose-dependent manner. Pretreatment with the CB(1) receptor subtype-selective antagonist AM 251 completely abolished the antinociceptive effect of WIN 55,212-2 whereas pretreatment with the CB(2) receptor subtype-selective antagonist AM 630 did not alter the antinociceptive effect of WIN 55,212-2. These data indicate that a CB(1)-selective agonist may be novel therapeutic treatment for clinical SCI pain.
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Acute systemic WIN55,212-2 suppressed spinal cord injury-induced mechanical allodynia in a CB1-dependent manner, although other parameters of neuropathic pain were not assessed.
“Acute WIN55,212-2, administered systemically, suppressed SCI-induced mechanical allodynia in a CB1-dependent manner, although other parameters of neuropathic pain were not assessed.103”
From Cannabinoids as Pharmacotherapies for Neuropathic Pain: From the Bench to the Bedside (2009) · cites this paper as [103]
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“The doses for WIN2, AM251 and AM630 were chosen based on effective doses in similar studies by others [19,32].”
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- Authors
- Hama, A., & Sagen, J.
- Affiliations
- University of Miami (US)
- Corresponding author
- Hama, Aldric
- Venue
- Experimental Neurology
- Publisher
- Elsevier BV
- Published
- 2007-03
- Volume
- 204
- Issue
- 1
- Pages
- 454-457
- ISSN
- 0014-4886 (ISSN-L)
- PMC
- PMC1861843
- OpenAlex
- W2123182126
- Cited by
- 40
- References
- 26
- Citations by year
- 2024: 1 · 2023: 2 · 2021: 4 · 2020: 1 · 2018: 4 · 2017: 1 · 2015: 1 · 2014: 3 · 2013: 3 · 2012: 2
- FWCI
- 1.88
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Experimental Neurology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute on Drug Abuse (DA-10546, R01DA010546)
- Topics
- Health Sciences › Medicine › Pharmacology › Cannabis and Cannabinoid Research
- Health Sciences › Medicine › Physiology › Pain Mechanisms and Treatments
- Health Sciences › Medicine › Pathology and Forensic Medicine › Spinal Cord Injury Research
- Keywords
- spinal cord injury, neuropathic pain, WIN 55,212-2, cannabinoid receptor agonists, CB1 receptor, CB2 receptor, tactile hypersensitivity, antinociception, withdrawal threshold, AM251, thoracic spinal cord compression
- MeSH terms
- Analgesics, Animals, Hindlimb, Hyperesthesia, Male, Morpholines, Naphthalenes, Nociceptors, Physical Stimulation, Piperidines, Pyrazoles, Spinal Cord Compression, Spinal Cord Injuries, Thoracic Vertebrae, Rats, Sprague-Dawley, Pain Threshold, Receptor, Cannabinoid, CB1, Benzoxazines, Rats, Cannabinoid Receptor Agonists
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