Cited by 269Cannabis and Cannabinoid ResearchPain Mechanisms and TreatmentsNeuroscience and Neuropharmacology Research

Cannabinoid CB1 Receptor Expression in Rat Spinal Cord

Farquhar-Smith, W.Paul, Egertová, Michaela, Bradbury, Elizabeth J., McMahon, Stephen B., Rice, Andrew S.C., Elphick, Maurice R.·2000·Molecular and Cellular Neuroscience

Abstract

While evidence implicates the endogenous cannabinoid system as a novel analgesic target at a spinal level, detailed analysis of the distribution of the cannabinoid receptor CB(1) in spinal cord has not been reported. Here, immunocytochemical studies were used to characterize the CB(1) receptor expression in rat spinal cord. Staining was found in the dorsolateral funiculus, the superficial dorsal horn (a double band of CB(1) immunoreactivity (ir) in laminae I and II inner/III transition), and lamina X. Although CB(1)-ir was present in the same laminae as primary afferent nociceptor markers, there was limited colocalization at an axonal level. Interruption of both primary afferent input by dorsal root rhizotomy and descending input by rostral spinal cord hemisection produced minor changes in CB(1)-ir. This and colocalization of CB(1)-ir with interneurons expressing protein kinase C subunit gamma-ir suggest that the majority of CB(1) expression is on spinal interneurons. These data provide a framework and implicate novel analgesic mechanisms for spinal actions of cannabinoids at the CB(1) receptor.

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Crossref · 10.1006/mcne.2000.0844

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Authors
Farquhar-Smith, W. P., Egertová, M., Bradbury, E. J., McMahon, S. B., Rice, A. S. C., & Elphick, M. R.
Affiliations
  • St Mary's Hospital (GB)
  • University of London (GB)
  • King's College London (GB)
Venue
Molecular and Cellular Neuroscience
Publisher
Elsevier BV
Published
2000-06
Volume
15
Issue
6
Pages
510-521
ISSN
1044-7431 (ISSN-L)
OpenAlex
W2137734852
Cited by
268
References
61
Citations by year
2026: 3 · 2025: 4 · 2024: 10 · 2023: 9 · 2022: 5 · 2021: 7 · 2020: 5 · 2019: 3 · 2018: 3 · 2017: 9 · 2016: 7 · 2015: 9 · 2014: 4 · 2013: 8 · 2012: 11
FWCI
12.31
Type
journal article
Language
en
Open access
closed
Locations
  • Molecular and Cellular Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
  • Research Portal (King's College London) (submittedVersion)
Topics
  • Health Sciences › Medicine › Pharmacology › Cannabis and Cannabinoid Research
  • Health Sciences › Medicine › Physiology › Pain Mechanisms and Treatments
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
CB1 receptor, spinal cord, endocannabinoid system, nociception, dorsal horn, laminae, primary afferent nociceptors, spinal cord hemisection, spinal interneurons, protein kinase C gamma, analgesia
MeSH terms
Animals, Antibodies, Cholera Toxin, Female, Fluorescent Antibody Technique, Interneurons, Male, Nerve Fibers, Nociceptors, Receptors, Drug, Spinal Cord Injuries, Spinal Nerve Roots, Blotting, Western, Rats, Wistar, Rhizotomy, Posterior Horn Cells, Receptors, Cannabinoid, Rats

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