Cannabinoid CB1 Receptor Expression in Rat Spinal Cord
Abstract
While evidence implicates the endogenous cannabinoid system as a novel analgesic target at a spinal level, detailed analysis of the distribution of the cannabinoid receptor CB(1) in spinal cord has not been reported. Here, immunocytochemical studies were used to characterize the CB(1) receptor expression in rat spinal cord. Staining was found in the dorsolateral funiculus, the superficial dorsal horn (a double band of CB(1) immunoreactivity (ir) in laminae I and II inner/III transition), and lamina X. Although CB(1)-ir was present in the same laminae as primary afferent nociceptor markers, there was limited colocalization at an axonal level. Interruption of both primary afferent input by dorsal root rhizotomy and descending input by rostral spinal cord hemisection produced minor changes in CB(1)-ir. This and colocalization of CB(1)-ir with interneurons expressing protein kinase C subunit gamma-ir suggest that the majority of CB(1) expression is on spinal interneurons. These data provide a framework and implicate novel analgesic mechanisms for spinal actions of cannabinoids at the CB(1) receptor.
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CB1 receptors are highly expressed on presynaptic neurons in the brain, spinal cord, and dorsal root ganglia.
“CB 1 receptors are highly expressed on presynaptic neurons in the brain (Tsou et al, 1998), spinal cord (Farquhar-Smith et al, 2000), and dorsal root ganglia (Hohmann and Herkenham, 1999;Sañudo-Peña et al, 1999).”
From The endogenous cannabinoid system: a budding source of targets for treating inflammatory and neuropathic pain (2018) · cites this paper as Farquhar-Smith et al, 2000
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- Authors
- Farquhar-Smith, W. P., Egertová, M., Bradbury, E. J., McMahon, S. B., Rice, A. S. C., & Elphick, M. R.
- Affiliations
- St Mary's Hospital (GB)
- University of London (GB)
- King's College London (GB)
- Venue
- Molecular and Cellular Neuroscience
- Publisher
- Elsevier BV
- Published
- 2000-06
- Volume
- 15
- Issue
- 6
- Pages
- 510-521
- ISSN
- 1044-7431 (ISSN-L)
- OpenAlex
- W2137734852
- Cited by
- 268
- References
- 61
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 10 · 2023: 9 · 2022: 5 · 2021: 7 · 2020: 5 · 2019: 3 · 2018: 3 · 2017: 9 · 2016: 7 · 2015: 9 · 2014: 4 · 2013: 8 · 2012: 11
- FWCI
- 12.31
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular and Cellular Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Research Portal (King's College London) (submittedVersion)
- Topics
- Health Sciences › Medicine › Pharmacology › Cannabis and Cannabinoid Research
- Health Sciences › Medicine › Physiology › Pain Mechanisms and Treatments
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- CB1 receptor, spinal cord, endocannabinoid system, nociception, dorsal horn, laminae, primary afferent nociceptors, spinal cord hemisection, spinal interneurons, protein kinase C gamma, analgesia
- MeSH terms
- Animals, Antibodies, Cholera Toxin, Female, Fluorescent Antibody Technique, Interneurons, Male, Nerve Fibers, Nociceptors, Receptors, Drug, Spinal Cord Injuries, Spinal Nerve Roots, Blotting, Western, Rats, Wistar, Rhizotomy, Posterior Horn Cells, Receptors, Cannabinoid, Rats
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