Cited by 98Genetically Modified Organisms ResearchCarcinogens and Genotoxicity AssessmentAgricultural safety and regulations

A three generation study with genetically modified Bt corn in rats: Biochemical and histopathological investigation

Kılıç, Aysun, Akay, M. Turan·2008·Food and Chemical Toxicology

Abstract

For the last ten years, in accordance with the increased use of genetically modified (GM) foods for human and livestock, a large number of feeding studies have been carried out. However, the evidence is still far from proving whether the long-term consumption of GM foods poses a possible danger for human or animal health. Therefore, this study was designed to evaluate the effects of transgenic corn on the rats that were fed through three generations with either GM corn or its conventional counterpart. Tissue samples of stomach, duodenum, liver and kidney were obtained for histopathological examinations. The average diameter of glomeruli, thickness of renal cortex and glomerular volume were calculated and number of affected animals/number of examined animals for liver and kidney histopathology were determined. Amounts of urea, urea nitrogen, creatinine, uric acid, total protein, albumin and globulin were determined; enzyme activities of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma glutamyltransferase, creatine kinase and amylase were measured in serum samples. No statistically significant differences were found in relative organ weights of rats within groups but there were some minimal histopathological changes in liver and kidney. Changes in creatinine, total protein and globulin levels were also determined in biochemical analysis.

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Crossref · 10.1016/j.fct.2007.11.016

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Authors
Kılıç, A., & Akay, M. T.
Affiliations
Hacettepe University (TR)
Venue
Food and Chemical Toxicology
Publisher
Elsevier BV
Published
2008-03
Volume
46
Issue
3
Pages
1164-1170
ISSN
0278-6915 (ISSN-L)
OpenAlex
W2046018773
Cited by
98
References
26
Citations by year
2026: 1 · 2025: 1 · 2024: 4 · 2023: 4 · 2022: 5 · 2021: 4 · 2020: 5 · 2019: 2 · 2018: 4 · 2017: 7 · 2016: 5 · 2015: 10 · 2014: 8 · 2013: 16 · 2012: 10
FWCI
0.81
Type
journal article
Language
en
Open access
closed
Locations
  • Food and Chemical Toxicology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Hacettepe Üniversitesi
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural safety and regulations
Keywords
Bt maize, genetically modified foods, transgenic maize, long-term dietary exposure, three-generation study, histopathology, liver histopathology, kidney histopathology, renal function, serum enzyme activity, renal function markers
MeSH terms
Animals, Blood Chemical Analysis, Body Weight, Zea mays, Duodenum, Female, Kidney, Liver, Male, Organ Size, Stomach, Rats, Wistar, Plants, Genetically Modified, Rats

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