A three generation study with genetically modified Bt corn in rats: Biochemical and histopathological investigation
Abstract
For the last ten years, in accordance with the increased use of genetically modified (GM) foods for human and livestock, a large number of feeding studies have been carried out. However, the evidence is still far from proving whether the long-term consumption of GM foods poses a possible danger for human or animal health. Therefore, this study was designed to evaluate the effects of transgenic corn on the rats that were fed through three generations with either GM corn or its conventional counterpart. Tissue samples of stomach, duodenum, liver and kidney were obtained for histopathological examinations. The average diameter of glomeruli, thickness of renal cortex and glomerular volume were calculated and number of affected animals/number of examined animals for liver and kidney histopathology were determined. Amounts of urea, urea nitrogen, creatinine, uric acid, total protein, albumin and globulin were determined; enzyme activities of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma glutamyltransferase, creatine kinase and amylase were measured in serum samples. No statistically significant differences were found in relative organ weights of rats within groups but there were some minimal histopathological changes in liver and kidney. Changes in creatinine, total protein and globulin levels were also determined in biochemical analysis.
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Studies longer than 90 days, including multigeneration and reproduction studies, have also been conducted on GM crops.
“Studies longer than 90 days, including multigeneration and reproduction studies, have also been conducted on GM crops (Brake et al., 2004; Brake & Evenson, 2004; Kiliç & Akay, 2008; Rhee et al., 2005; Snell et al., 2012; Velimirov et al., 2008; Wainwright et al., 2003).”
From The use of whole food animal studies in the safety assessment of genetically modified crops: Limitations and recommendations (2013) · cites this paper as Kiliç & Akay, 2008
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Application of biotechnology in chemical industry
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- Authors
- Kılıç, A., & Akay, M. T.
- Affiliations
- Hacettepe University (TR)
- Venue
- Food and Chemical Toxicology
- Publisher
- Elsevier BV
- Published
- 2008-03
- Volume
- 46
- Issue
- 3
- Pages
- 1164-1170
- ISSN
- 0278-6915 (ISSN-L)
- OpenAlex
- W2046018773
- Cited by
- 98
- References
- 26
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 4 · 2023: 4 · 2022: 5 · 2021: 4 · 2020: 5 · 2019: 2 · 2018: 4 · 2017: 7 · 2016: 5 · 2015: 10 · 2014: 8 · 2013: 16 · 2012: 10
- FWCI
- 0.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Food and Chemical Toxicology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Hacettepe Üniversitesi
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural safety and regulations
- Keywords
- Bt maize, genetically modified foods, transgenic maize, long-term dietary exposure, three-generation study, histopathology, liver histopathology, kidney histopathology, renal function, serum enzyme activity, renal function markers
- MeSH terms
- Animals, Blood Chemical Analysis, Body Weight, Zea mays, Duodenum, Female, Kidney, Liver, Male, Organ Size, Stomach, Rats, Wistar, Plants, Genetically Modified, Rats
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