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Catechin-based extract optimization obtained from Arbutus unedo L. fruits using maceration/microwave/ultrasound extraction techniques
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This study compares three extraction techniques (maceration, microwave and ultrasound) for catechin recover from Arbutus unedo fruit extracts. To obtain the conditions that maximize catechin extraction yield, a response surface methodology was applied using a 3-level full factorial Box–Behnken design in which the processing time (t), temperature (T), ultrasonic power (W) and ethanol percentage (Et%) were the relevant independent variables with the response (catechin content, mg/g dw) measured by HPLC-PDA. A fixed solid/solvent ratio of 50g/L was used in all techniques. Maceration and microwave extractions were found to be the most effective methods, capable of yielding 1.38±0.1 and 1.70±0.3mg/g dw of catechin, respectively at the optimal extraction conditions. The optimal conditions for maceration were 93.2±3.7min, 79.6±5.2°C and 23.1±3.7% of ethanol, while for the microwave extraction were 42.2±4.1min, 137.1±8.1°C and 12.1±1.1% of ethanol. Comparatively with maceration, the microwave system was a faster solution, conducting to slightly higher catechin yields, but using higher temperatures to reach similar values. The ultrasound method was the least effective solution, yielding 0.71±0.1mg/g dw of catechin at 42.4±3.6min, 314.9±21.2W and 40.3±3.8% ethanol. The results highlight the potential of using A. unedo fruits bio-residues as a productive source of catechin.
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“Their structure corresponds to that of hydrophobic tetraterpenes with a structure of branched hydrocarbons with a methyl group bound at C-40 [144,145].”
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Metadata
- Authors
- Albuquerque, B. R., Prieto, M. A., Barreiro, M. F., Rodrigues, A., Curran, T. P., Barros, L., & Ferreira, I. C. F. R.
- Affiliations
- Universidade Tecnológica Federal do Paraná (BR)
- Universidade de Vigo (ES)
- Universidade Politécnica de Bragança (PT)
- Universidade do Porto (PT)
- University College Dublin (IE)
- Corresponding author
- Ferreira, Isabel C.F.R.
- ORCID
- Venue
- Industrial Crops and Products
- Publisher
- Elsevier BV
- Published
- 2017-01
- Volume
- 95
- Pages
- 404-415
- ISSN
- 0926-6690 (ISSN-L)
- OpenAlex
- W2548500750
- Cited by
- 154
- References
- 57
- Citations by year
- 2026: 8 · 2025: 20 · 2024: 15 · 2023: 20 · 2022: 17 · 2021: 23 · 2020: 21 · 2019: 10 · 2018: 13 · 2017: 7
- FWCI
- 7.86
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- cc-by-nc-nd
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- Locations
- Industrial Crops and Products (publishedVersion)
- Research Repository UCD (University College Dublin) (submittedVersion, cc-by-nc-nd)
- Arrow@dit (Dublin Institute of Technology) (submittedVersion)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, other-oa)
- Funders
- Foundation for Science and Technology (FCT, Portugal) (UID/AGR/00690/2013, SFRH/BPD/107855/2015)
- FEDER
- Topics
- Health Sciences › Medicine › Biochemistry (medical) › Phytochemicals and Antioxidant Activities
- Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural and Biological Research
- Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
- Keywords
- Arbutus unedo, cranberry, catechins, maceration, microwave-assisted extraction, ultrasound-assisted extraction, ethanol concentration, extraction temperature, extraction time, polyphenols
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