Identification of Dirofilaria immitis immunoreactive proteins recognized by sera from infected cats using two-dimensional electrophoresis and mass spectrometry
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The aim of the present work was to identify proteins of Dirofilaria immitis recognized by the immune system of naturally and experimentally infected cats, using two-dimensional electrophoresis and mass spectrometry. Thirty-five immunoreactive proteins of D. immitis were identified. These proteins are involved in metabolism, plasminogen binding, anti-oxidant and detoxificant activity, up-regulation of the Th2 anti-inflammatory response and other processes. The timing evolution of this recognition pattern indicated that at 2 months post-infection a wide recognition of many parasite proteins belonging to many functional groups is still observed, increasing progressively during the course of the infection. The real effect on the vital capacity of D. immitis worms and on the development of pathological events of feline dirofilariosis will be investigated in the future.
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Proteomics with mass spectrometry allowed the simultaneous identification of numerous proteins, comprising 39 proteins from D. immitis and 15 from D. repens, many represented by several isoforms.
“Together with mass spectrometry, proteomics allowed the simultaneous identification of numerous proteins, comprising 39 proteins from D. immitis and 15 from D. repens, many of which were represented by several isoforms (157,158,313).”
From Human and animal dirofilariasis: The emergence of a zoonotic mosaic (2012) · cites this paper as [157]
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Human and animal dirofilariasis: The emergence of a zoonotic mosaic
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Pană, D., Rădulescu, A., Mitrea, I. L., & Ionita, M. (2020) · Helminthologia · doi:10.2478/helm-2020-0009 · Cited by 14 · see citing works
- Authors
- González-Miguel, J., Morchón, R., Siles-Lucas, M., Oleaga, A., & Simón, F.
- Affiliations
- Universidad de Salamanca (ES)
- Instituto de Recursos Naturales y Agrobiología de Salamanca (ES)
- Venue
- Molecular and Biochemical Parasitology
- Publisher
- Elsevier BV
- Published
- 2010-11
- Volume
- 174
- Issue
- 1
- Pages
- 78-82
- ISSN
- 0166-6851 (ISSN-L)
- OpenAlex
- W2048596689
- Cited by
- 12
- References
- 21
- Citations by year
- 2024: 1 · 2022: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 1 · 2015: 1 · 2014: 2 · 2013: 1 · 2012: 1
- FWCI
- 0.62
- Type
- journal article
- Language
- en
- Open access
- green
- api.elsevier.com
- Licence
- cc-by-nc-nd
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- Locations
- Molecular and Biochemical Parasitology (publishedVersion)
- PubMed (publishedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion, cc-by-nc-nd)
- Funders
- Agencia de Desarrollo Económico de Castilla y León (SA090/A09)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
- Health Sciences › Veterinary › Small Animals › Helminth infection and control
- Life Sciences › Immunology and Microbiology › Parasitology › Parasites and Host Interactions
- Keywords
- Dirofilaria immitis, immunoreactive proteins, parasite antigens, cats, host immune response, plasminogen binding, antioxidant activity, Th2 immune response, parasite development, serological diagnosis
- MeSH terms
- Animals, Antibodies, Helminth, Antigens, Helminth, Cat Diseases, Cats, Dirofilaria immitis, Dirofilariasis, Mass Spectrometry, Electrophoresis, Gel, Two-Dimensional, Helminth Proteins
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