Cited by 1,391Epigenetics and DNA MethylationCancer-related molecular mechanisms researchGenomics and Chromatin Dynamics

Molecular Interplay of the Noncoding RNA ANRIL and Methylated Histone H3 Lysine 27 by Polycomb CBX7 in Transcriptional Silencing of INK4a

Open access
Yap, Kyoko L., Li, Side, Muñoz-Cabello, Ana M., Raguz, Selina, Zeng, Lei, Mujtaba, Shiraz, Gil, Jesús, Walsh, Martin J. +1 more·2010·Molecular Cell

Abstract

Expression of the INK4b/ARF/INK4a tumor suppressor locus in normal and cancerous cell growth is controlled by methylation of histone H3 at lysine 27 (H3K27me) as directed by the Polycomb group proteins. The antisense noncoding RNA ANRIL of the INK4b/ARF/INK4a locus is also important for expression of the protein-coding genes in cis, but its mechanism has remained elusive. Here we report that chromobox 7 (CBX7) within the polycomb repressive complex 1 binds to ANRIL, and both CBX7 and ANRIL are found at elevated levels in prostate cancer tissues. In concert with H3K27me recognition, binding to RNA contributes to CBX7 function, and disruption of either interaction impacts the ability of CBX7 to repress the INK4b/ARF/INK4a locus and control senescence. Structure-guided analysis reveals the molecular interplay between noncoding RNA and H3K27me as mediated by the conserved chromodomain. Our study suggests a mechanism by which noncoding RNA participates directly in epigenetic transcriptional repression.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1016/j.molcel.2010.03.021

Cites

62 of 62 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • In proliferative cells, polycomb repressive complexes PRC1 and PRC2 are recruited by the long noncoding RNA ANRIL to the CDKN2A locus.

    “In proliferative cells, polycomb repressive complexes (PRC1 and PRC2) are recruited by the long noncoding RNA ANRIL to the CDKN2A locus [79].”

    From Mechanisms and Regulation of Cellular Senescence (2021) · cites this paper as [79]

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

1 retracted

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Yap, K. L., Li, S., Muñoz-Cabello, A. M., Raguz, S., Zeng, L., Mujtaba, S., Gil, J., Walsh, M. J., & Zhou, M.-M.
Affiliations
  • Icahn School of Medicine at Mount Sinai (US)
  • Imperial College London (GB)
Corresponding author
  • Walsh, Martin J.
  • Zhou, Ming-Ming
Venue
Molecular Cell
Publisher
Elsevier BV
Published
2010-06
Volume
38
Issue
5
Pages
662-674
ISSN
1097-2765 (ISSN-L)
OpenAlex
W2052741109
Cited by
1,391
References
62
Citations by year
2026: 27 · 2025: 43 · 2024: 44 · 2023: 54 · 2022: 61 · 2021: 86 · 2020: 78 · 2019: 94 · 2018: 112 · 2017: 113 · 2016: 120 · 2015: 129 · 2014: 122 · 2013: 116 · 2012: 104
FWCI
40.29
Type
journal article
Language
en
Open access
Locations
  • Molecular Cell (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institute on Drug Abuse (RC1DA028776)
  • National Human Genome Research Institute (R01HG004508)
  • National Cancer Institute (R01CA087658)
  • National Institute of General Medical Sciences (R01GM073207)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords
ANRIL, CBX7, PRC1, INK4A, transcriptional silencing, chromodomain, prostate cancer, cellular senescence
MeSH terms
Animals, Multigene Family, Histones, Humans, Lysine, Male, Methylation, Models, Molecular, Molecular Sequence Data, Prostatic Neoplasms, Repressor Proteins, Transcription, Genetic, Cellular Senescence, Protein Structure, Tertiary, Nuclear Magnetic Resonance, Biomolecular, Cyclin-Dependent Kinase Inhibitor p16, Gene Silencing, RNA, Untranslated, Cell Line, Tumor, Cyclin-Dependent Kinase Inhibitor p15, Polycomb-Group Proteins, Polycomb Repressive Complex 1

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review