REM sleep deprivation increases the expression of interleukin genes in mice hypothalamus
Abstract
Recently, evidence has suggested the possible involvement of inflammatory cytokines in sleep deprivation (SD). In this study, we assessed the patterns of inflammatory gene regulation in the hypothalamus of REM SD mice. C57BL/6 mice were randomly assigned to two groups, SD (n=15) and control groups (n=15). Mice in the SD group were sleep-deprived for 72h using modified multiple platforms. Microarray analysis on inflammatory genes was performed in mice hypothalamus. In addition, interleukin 1 beta (IL1β) protein expression was analyzed by the immunochemistry method. Through microarray analysis, we found that expressions of IL subfamily genes, such as IL1β (2.55-fold), IL18 (1.92-fold), IL11 receptor alpha chain 1 (1.48-fold), IL5 (1.41-fold), and IL17E genes (1.31-fold), were up-regulated in the hypothalamus of SD mice compared to the control. The increase in the expression of these genes was also confirmed by RT-PCR. Among these genes, the expression of IL1β was particularly increased in the hypothalamus of SD mice. Interestingly, we found that the protein expression of endogenous IL1β was also elevated in the hypothalamus of SD mice compared to the control mice. These results implicate that IL subfamily genes, and in particular, IL1β, may play a role in sleep regulation in the hypothalamus of REM SD mice.
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Comparable effects of anti-TNF therapy on sleep were found in patients with IBD and ankylosing spondylitis.
“Comparable effects of anti-TNF therapy on sleep were found in patients with IBD and ankylosing spondylitis (282, 527).”
From The Sleep-Immune Crosstalk in Health and Disease (2019) · cites this paper as [282]
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The Sleep-Immune Crosstalk in Health and Disease
Besedovsky, L., Lange, T., & Haack, M. (2019) · Physiological Reviews · doi:10.1152/physrev.00010.2018 · Cited by 1,479 · see citing works
“However, an increase at the mRNA level after prolonged sleep restriction has been reported in humans (556), as well as in animal studies measuring gene expression in peripheral blood lymphocytes (245), and in the spleen, liver, CNS, heart, and adipose tissue (18, 162, 281, 290, 348).”
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- Authors
- Kang, W. S., Park, H. J., Chung, J.-H., & Kim, J. W.
- Affiliations
- Kyung Hee University (KR)
- Corresponding author
- Kim, Jong Woo
- Venue
- Neuroscience Letters
- Publisher
- Elsevier BV
- Published
- 2013-11
- Volume
- 556
- Pages
- 73-78
- ISSN
- 0304-3940 (ISSN-L)
- OpenAlex
- W2062000743
- Cited by
- 33
- References
- 31
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 4 · 2023: 4 · 2022: 5 · 2021: 2 · 2020: 2 · 2019: 4 · 2018: 1 · 2017: 4 · 2015: 4 · 2013: 1
- FWCI
- 0.91
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Kyung Hee University (KHU-20090641)
- Topics
- Life Sciences › Neuroscience › Cognitive Neuroscience › Sleep and Wakefulness Research
- Social Sciences › Psychology › Experimental and Cognitive Psychology › Sleep and Work-Related Fatigue
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- REM sleep deprivation, interleukin genes, hypothalamus, IL-1β, inflammatory cytokines, sleep regulation, IL-18, IL-5, gene expression
- MeSH terms
- Animals, Hypothalamus, Interleukins, Male, Mice, Inbred C57BL, Random Allocation, Sleep Deprivation, Sleep, REM, Up-Regulation, Tissue Array Analysis, Mice, Transcriptome
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