Cited by 87Nicotinic Acetylcholine Receptors StudyNeurotransmitter Receptor Influence on BehaviorSmoking Behavior and Cessation

Reinforcing effects of nicotine microinjections into the ventral tegmental area of mice: dependence on cholinergic nicotinic and dopaminergic D1 receptors

David, Vincent, Besson, Morgane, Changeux, Jean-Pierre, Granon, Sylvie, Cazala, Pierre·2006·Neuropharmacology

Abstract

We used an intracranial self-administration (ICSA) procedure to assess the involvement of the ventral tegmental area (VTA) nicotinic receptors in the rewarding effects of nicotine. We then challenged intra-VTA nicotine self-administration via systemic or local injections of dopamine (DA)-D1 and nicotinic receptor antagonists. C57BL/6J mice were stereotaxically implanted unilaterally with a guide cannula above the VTA. After 1 week of recovery, mice were allowed to discriminate between two arms of a Y-maze over seven daily sessions, one arm being reinforced by intracranial nicotine microinjection. Mice exhibited nicotine self-administration at both doses tested, i.e. 10 ng (21.6 pmol) and 100 ng (216 pmol)/50-nl injection. In contrast, mice receiving a 216-pmol nicotine dose 0.8 mm above VTA performed at chance level. Once the ICSA response was acquired, systemic pretreatment with the DA-D1 receptor antagonist SCH 23390 (25 microg/kg i.p.) or co-infusion of the nAChR antagonist DHbetaE with nicotine disrupted ICSA. Replacement of SCH 23390 by vehicle, or withdrawal of DHbetaE from nicotine/DHbetaE mixed solutions led to recovery of intra-VTA nicotine self-administration. We conclude that nicotinic receptors in the VTA, presumably alpha4beta2 nAChRs are critically to mediate the rewarding effects of nicotine and that DA-D1 receptors are also directly implicated.

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Crossref · 10.1016/j.neuropharm.2006.02.003

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Authors
David, V., Besson, M., Changeux, J.-P., Granon, S., & Cazala, P.
Affiliations
Centre National de la Recherche Scientifique (FR)
Corresponding author
Granon, Sylvie
Venue
Neuropharmacology
Publisher
Elsevier BV
Published
2006-06
Volume
50
Issue
8
Pages
1030-1040
ISSN
0028-3908 (ISSN-L)
OpenAlex
W2139155720
Cited by
87
References
58
Citations by year
2024: 1 · 2023: 1 · 2022: 1 · 2021: 5 · 2020: 2 · 2019: 3 · 2018: 1 · 2017: 2 · 2016: 1 · 2015: 4 · 2014: 6 · 2013: 6 · 2012: 8
FWCI
4.32
Type
journal article
Language
en
Open access
closed
Locations
  • Neuropharmacology (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Funders
Institut Carnot Santé Animale
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
  • Health Sciences › Medicine › Physiology › Smoking Behavior and Cessation
Keywords
nicotine, ventral tegmental area, nicotinic acetylcholine receptors, dopamine D1 receptor, nicotine self-administration, alpha4beta2 nAChR, SCH 23390, reward, cholinergic-dopaminergic interaction
MeSH terms
Animals, Behavior, Animal, Benzazepines, Dihydro-beta-Erythroidine, Dose-Response Relationship, Drug, Drug Combinations, Drug Interactions, Male, Mice, Inbred C57BL, Microinjections, Nicotine, Reaction Time, Receptors, Nicotinic, Reinforcement, Psychology, Self Administration, Spatial Behavior, Receptors, Dopamine D1, Ventral Tegmental Area, Dopamine Antagonists, Nicotinic Agonists, Nicotinic Antagonists, Maze Learning, Mice

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