Cited by 58Air Quality and Health ImpactsDermatology and Skin DiseasesSkin Protection and Aging

Transcriptome analysis of airborne PM 2.5-induced detrimental effects on human keratinocytes

Kim, Hyoung-June, Bae, Il-Hong, Son, Eui Dong, Park, Juyearl, Cha, Nari, Na, Hye-Won, Jung, Changjo, Go, You-Seak +3 more·2017·Toxicology Letters

Abstract

Ambient air pollution is becoming more severe worldwide, posing a serious threat to human health. Fine airborne particles of particulate matter (PM 2.5) show higher cytotoxicity than other coarse fractions. Indeed, PM 2.5 induces cardiovascular or respiratory damage; however, few studies have evaluated the detrimental effect of PM 2.5 to normal human skin. We used a next-generation sequencing-based (RNA-Seq) method with transcriptome and Gene Ontology (GO) enrichment analysis to determine the harmful influences of PM 2.5 on human normal epidermal keratinocytes. DAVID analysis showed that the most significantly enriched GO terms were associated with epidermis-related biological processes such as "epidermis development (GO: 0008544)" and "keratinocyte differentiation (GO: 0030216)", suggesting that PM 2.5 has some deleterious effects to the human epidermis. In addition, Ingenuity Pathway Analysis predicted inflammation-related signaling as one of the major PM 2.5-induced signaling pathways, and pro-inflammatory cytokines as upstream regulators with symptoms similar to psoriasis as downstream effects. PM 2.5 caused considerable changes in the expression of pro-inflammatory cytokines and psoriatic skin disease-related genes, might lead to epidermal dysfunctions. Our results might help to understand the mechanism of air pollution-induced skin barrier perturbation and contribute to the development of a new strategy for the prevention or recovery of the consequent damage.

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Crossref · 10.1016/j.toxlet.2017.03.010

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Authors
Kim, H.-J., Bae, I.-H., Son, E. D., Park, J., Cha, N., Na, H.-W., Jung, C., Go, Y.-S., Kim, D.-Y., Lee, T. R., & Shin, D. W.
Affiliations
  • Amorepacific (South Korea) (KR)
  • Seoul National University (KR)
  • Macrogen (South Korea) (KR)
Corresponding author
  • Lee, Tae Ryong
  • Shin, Dong Wook
Venue
Toxicology Letters
Publisher
Elsevier BV
Published
2017-05
Volume
273
Pages
26-35
ISSN
0378-4274 (ISSN-L)
OpenAlex
W2598483734
Cited by
58
References
54
Citations by year
2026: 5 · 2025: 6 · 2024: 9 · 2023: 7 · 2022: 12 · 2021: 6 · 2020: 5 · 2019: 6 · 2018: 1 · 2017: 1
FWCI
1.92
Type
journal article
Language
en
Open access
closed
Locations
  • Toxicology Letters (publishedVersion)
  • PubMed (publishedVersion)
Funders
Hankuk University of Foreign Studies
Topics
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Air Quality and Health Impacts
  • Health Sciences › Medicine › Dermatology › Dermatology and Skin Diseases
  • Health Sciences › Medicine › Dermatology › Skin Protection and Aging
Keywords
PM2.5, particulate matter, air pollution, human keratinocytes, epidermal development, inflammatory cytokines, psoriasis, skin barrier dysfunction, inflammatory signaling, transcriptome
MeSH terms
Air Pollutants, Cell Survival, Cells, Cultured, Epidermis, Humans, Particle Size, Psoriasis, Keratinocytes, Cytokines, Gene Expression Profiling, Particulate Matter, Transcriptome

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