Transcriptome analysis of airborne PM 2.5-induced detrimental effects on human keratinocytes
Abstract
Ambient air pollution is becoming more severe worldwide, posing a serious threat to human health. Fine airborne particles of particulate matter (PM 2.5) show higher cytotoxicity than other coarse fractions. Indeed, PM 2.5 induces cardiovascular or respiratory damage; however, few studies have evaluated the detrimental effect of PM 2.5 to normal human skin. We used a next-generation sequencing-based (RNA-Seq) method with transcriptome and Gene Ontology (GO) enrichment analysis to determine the harmful influences of PM 2.5 on human normal epidermal keratinocytes. DAVID analysis showed that the most significantly enriched GO terms were associated with epidermis-related biological processes such as "epidermis development (GO: 0008544)" and "keratinocyte differentiation (GO: 0030216)", suggesting that PM 2.5 has some deleterious effects to the human epidermis. In addition, Ingenuity Pathway Analysis predicted inflammation-related signaling as one of the major PM 2.5-induced signaling pathways, and pro-inflammatory cytokines as upstream regulators with symptoms similar to psoriasis as downstream effects. PM 2.5 caused considerable changes in the expression of pro-inflammatory cytokines and psoriatic skin disease-related genes, might lead to epidermal dysfunctions. Our results might help to understand the mechanism of air pollution-induced skin barrier perturbation and contribute to the development of a new strategy for the prevention or recovery of the consequent damage.
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Fine airborne PM activated the aryl hydrocarbon receptor (AHR) that resulted in detrimental effects on human keratinocytes.
“fine airborne PM activated the aryl hydrocarbon receptor (AHR) that resulted in detrimental effects on human keratinocytes [58,59]”
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Self-citation
- Authors
- Kim, H.-J., Bae, I.-H., Son, E. D., Park, J., Cha, N., Na, H.-W., Jung, C., Go, Y.-S., Kim, D.-Y., Lee, T. R., & Shin, D. W.
- Affiliations
- Amorepacific (South Korea) (KR)
- Seoul National University (KR)
- Macrogen (South Korea) (KR)
- Corresponding author
- Lee, Tae Ryong
- Shin, Dong Wook
- Venue
- Toxicology Letters
- Publisher
- Elsevier BV
- Published
- 2017-05
- Volume
- 273
- Pages
- 26-35
- ISSN
- 0378-4274 (ISSN-L)
- OpenAlex
- W2598483734
- Cited by
- 58
- References
- 54
- Citations by year
- 2026: 5 · 2025: 6 · 2024: 9 · 2023: 7 · 2022: 12 · 2021: 6 · 2020: 5 · 2019: 6 · 2018: 1 · 2017: 1
- FWCI
- 1.92
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Toxicology Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Hankuk University of Foreign Studies
- Topics
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Air Quality and Health Impacts
- Health Sciences › Medicine › Dermatology › Dermatology and Skin Diseases
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Keywords
- PM2.5, particulate matter, air pollution, human keratinocytes, epidermal development, inflammatory cytokines, psoriasis, skin barrier dysfunction, inflammatory signaling, transcriptome
- MeSH terms
- Air Pollutants, Cell Survival, Cells, Cultured, Epidermis, Humans, Particle Size, Psoriasis, Keratinocytes, Cytokines, Gene Expression Profiling, Particulate Matter, Transcriptome
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