The role of nitric oxide and cGMP in platelet adhesion to vascular endothelium
Abstract
The inhibition of platelet adhesion by nitric oxide (NO) and prostacyclin and their mechanism of action was studied. Platelet adhesion to collagen fibrils and endothelial cell matrix was inhibited completely by NO but only partially by prostacyclin. Adhesion of platelets to endothelial cell monolayers was inhibited by bradykinin. This effect of bradykinin was unaffected by aspirin, and was accounted for by the amounts of NO released by the endothelial cells. Inhibition of platelet adhesion by NO and prostacyclin was potentiated by selective inhibitors of cGMP phosphodiesterase, but not of cAMP phosphodiesterase, indicating that elevation of cGMP regulates platelet adhesion.
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Sodium nitrite administration resulted in vascular smooth muscle relaxation in pre-contracted rat mesenteric arteries via cGMP production, an effect amplified in the presence of exogenous guanosine triphosphate.
“Laustiola et al. (103) showed that sodium nitrite administration resulted in vascular smooth muscle relaxation in pre-contracted rat mesenteric arteries via cGMP production, an effect that was amplified in the presence of exogenous guanosine triphosphate.”
From The effects of dietary nitrate on blood pressure and endothelial function: a review of human intervention studies (2013) · cites this paper as [103]
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- Authors
- Radomski, M. W., Palmer, R. M. J., & Moncada, S.
- Affiliations
- Wellcome Trust (GB)
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 1987-11
- Volume
- 148
- Issue
- 3
- Pages
- 1482-1489
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W1967225427
- Cited by
- 746
- References
- 19
- Citations by year
- 2025: 5 · 2024: 5 · 2023: 6 · 2022: 10 · 2021: 10 · 2020: 13 · 2019: 7 · 2018: 20 · 2017: 11 · 2016: 20 · 2015: 13 · 2014: 21 · 2013: 12 · 2012: 15
- FWCI
- 11.49
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Hematology › Platelet Disorders and Treatments
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Keywords
- nitric oxide, cyclic GMP, platelet adhesion, vascular endothelium, prostacyclin, bradykinin, collagen fibrils, phosphodiesterase inhibitors, aspirin, cyclic AMP
- MeSH terms
- Aspirin, Blood Platelets, Collagen, Endothelium, Vascular, Extracellular Matrix, Cyclic GMP, Humans, Nitric Oxide, Phthalazines, Platelet Adhesiveness, Epoprostenol, Purinones, Superoxide Dismutase, Thrombin, 3',5'-Cyclic-GMP Phosphodiesterases, In Vitro Techniques
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