Cited by 154Mangiferin and Mango ExtractsPhytochemicals and Antioxidant ActivitiesNatural Compound Pharmacology Studies

In vitro effects of mangiferin on superoxide concentrations and expression of the inducible nitric oxide synthase, tumour necrosis factor-α and transforming growth factor-β genes

Leiro, José Manuel, Álvarez, Ezequiel, Arranz, Juan Alberto, Siso, Isabel González, Orallo, Francisco·2003·Biochemical Pharmacology

Abstract

This study investigated the effects of the natural polyphenol mangiferin (MA) on superoxide anion (O(2)(-)) production, xanthine oxidase (XO) activity, vascular contractility, inducible nitric oxide synthase (iNOS) mRNA levels, tumour necrosis factor-alpha (TNF-alpha) mRNA levels, and tumour growth factor-beta (TGF-beta) mRNA levels. O(2)(-) was generated by the hypoxanthine-xanthine oxidase (HX-XO) and phenazine methosulphate (PMS)-NADH systems. XO activity was determined by measurement of uric acid production with xanthine as substrate. Vascular contraction experiments were performed with intact rat aortic rings. iNOS, TNF-alpha and TGF-beta gene expression in rat macrophages stimulated in vivo with 3% thioglycollate and in vitro with 100 ng/mL lipopolysaccharide and 10U/mL of interferon-gamma were evaluated semiquantitatively by the retrotranscriptase-polymerase chain reaction. MA at 10-100 microM, like the known O(2)(-) scavenger superoxide dismutase (1U/mL), scavenged O(2)(-) produced by the HX/XO and PMS-NADH systems. By contrast MA at 1-100 microM, unlike allopurinol (10 microM), was unable to inhibit XO activity. MA at 1-100 microM did not modify resting tone or the contractile responses elicited by 1 microM phenylephrine or 1 microM phorbol 12-myristate 13-acetate in rat aorta. MA at 1-100 microM, like dexamethasone (100 microM), decreased iNOS mRNA levels in activated macrophages. At 100 microM, MA also reduced TNF-alpha mRNA levels, but increased TGF-beta mRNA levels. These results thus indicate that MA is an O(2)(-) scavenger and that it inhibits expression of the iNOS and TNF-alpha genes, suggesting that it may be of potential value in the treatment of inflammatory and/or neurodegenerative disorders. In addition, the finding that MA enhances TGF-beta gene expression suggests that this polyphenol might also be of value in the prevention of cancer, autoimmune disorders, atherosclerosis and coronary heart disease.

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Crossref · 10.1016/s0006-2952(03)00041-8

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  1. Ding, 1990, Macrophage deactivating factor and transforming growth factors β1, β2 and β3 inhibit catalytic activity, Neuropharmacology, 33, 1245

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Authors
Leiro, J. M., Álvarez, E., Arranz, J. A., Siso, I. G., & Orallo, F.
Affiliations
Universidade de Santiago de Compostela (ES)
Corresponding author
Leiro, José Manuel
Venue
Biochemical Pharmacology
Publisher
Elsevier BV
Published
2003-04
Volume
65
Issue
8
Pages
1361-1371
ISSN
0006-2952 (ISSN-L)
OpenAlex
W2013744651
Cited by
154
References
73
Citations by year
2026: 1 · 2025: 3 · 2024: 2 · 2023: 5 · 2022: 2 · 2021: 6 · 2020: 2 · 2019: 4 · 2018: 4 · 2017: 3 · 2016: 9 · 2015: 17 · 2014: 12 · 2013: 10 · 2012: 8
FWCI
19.20
Type
journal article
Language
en
Open access
closed
Locations
  • Biochemical Pharmacology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Complementary and alternative medicine › Mangiferin and Mango Extracts
  • Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Natural Compound Pharmacology Studies
Keywords
mangiferin, superoxide radical scavenging, xanthine oxidase, inducible nitric oxide synthase, TNF-α, TGF-beta, macrophages, vascular contractility, polyphenols, inflammation, neurodegenerative diseases, gene expression regulation, lipopolysaccharide
MeSH terms
Animals, Gene Expression Regulation, Male, Superoxides, Tumor Necrosis Factor-alpha, Xanthenes, Transforming Growth Factor beta, Rats, Wistar, Macrophages, Peritoneal, Nitric Oxide Synthase, Reverse Transcriptase Polymerase Chain Reaction, Xanthones, Rats, Nitric Oxide Synthase Type II

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