In vitro effects of mangiferin on superoxide concentrations and expression of the inducible nitric oxide synthase, tumour necrosis factor-α and transforming growth factor-β genes
Abstract
This study investigated the effects of the natural polyphenol mangiferin (MA) on superoxide anion (O(2)(-)) production, xanthine oxidase (XO) activity, vascular contractility, inducible nitric oxide synthase (iNOS) mRNA levels, tumour necrosis factor-alpha (TNF-alpha) mRNA levels, and tumour growth factor-beta (TGF-beta) mRNA levels. O(2)(-) was generated by the hypoxanthine-xanthine oxidase (HX-XO) and phenazine methosulphate (PMS)-NADH systems. XO activity was determined by measurement of uric acid production with xanthine as substrate. Vascular contraction experiments were performed with intact rat aortic rings. iNOS, TNF-alpha and TGF-beta gene expression in rat macrophages stimulated in vivo with 3% thioglycollate and in vitro with 100 ng/mL lipopolysaccharide and 10U/mL of interferon-gamma were evaluated semiquantitatively by the retrotranscriptase-polymerase chain reaction. MA at 10-100 microM, like the known O(2)(-) scavenger superoxide dismutase (1U/mL), scavenged O(2)(-) produced by the HX/XO and PMS-NADH systems. By contrast MA at 1-100 microM, unlike allopurinol (10 microM), was unable to inhibit XO activity. MA at 1-100 microM did not modify resting tone or the contractile responses elicited by 1 microM phenylephrine or 1 microM phorbol 12-myristate 13-acetate in rat aorta. MA at 1-100 microM, like dexamethasone (100 microM), decreased iNOS mRNA levels in activated macrophages. At 100 microM, MA also reduced TNF-alpha mRNA levels, but increased TGF-beta mRNA levels. These results thus indicate that MA is an O(2)(-) scavenger and that it inhibits expression of the iNOS and TNF-alpha genes, suggesting that it may be of potential value in the treatment of inflammatory and/or neurodegenerative disorders. In addition, the finding that MA enhances TGF-beta gene expression suggests that this polyphenol might also be of value in the prevention of cancer, autoimmune disorders, atherosclerosis and coronary heart disease.
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- Authors
- Leiro, J. M., Álvarez, E., Arranz, J. A., Siso, I. G., & Orallo, F.
- Affiliations
- Universidade de Santiago de Compostela (ES)
- Corresponding author
- Leiro, José Manuel
- Venue
- Biochemical Pharmacology
- Publisher
- Elsevier BV
- Published
- 2003-04
- Volume
- 65
- Issue
- 8
- Pages
- 1361-1371
- ISSN
- 0006-2952 (ISSN-L)
- OpenAlex
- W2013744651
- Semantic Scholar
- 686b2b9c17a3c420ea4a4747dc177e0df90c99be
- Cited by
- 154
- References
- 73
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 2 · 2023: 5 · 2022: 2 · 2021: 6 · 2020: 2 · 2019: 4 · 2018: 4 · 2017: 3 · 2016: 9 · 2015: 17 · 2014: 12 · 2013: 10 · 2012: 8
- FWCI
- 19.20
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical Pharmacology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Mangiferin and Mango Extracts
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Natural Compound Pharmacology Studies
- Keywords
- mangiferin, superoxide radical scavenging, xanthine oxidase, inducible nitric oxide synthase, TNF-α, TGF-beta, macrophages, vascular contractility, polyphenols, inflammation, neurodegenerative diseases, gene expression regulation, lipopolysaccharide
- MeSH terms
- Animals, Gene Expression Regulation, Male, Superoxides, Tumor Necrosis Factor-alpha, Xanthenes, Transforming Growth Factor beta, Rats, Wistar, Macrophages, Peritoneal, Nitric Oxide Synthase, Reverse Transcriptase Polymerase Chain Reaction, Xanthones, Rats, Nitric Oxide Synthase Type II
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