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Cited by 167Sleep and Wakefulness ResearchSleep and Work-Related FatigueMemory and Neural Mechanisms

Sleep deprivation by the “flower pot” technique and spatial reference memory

Youngblood, Bradley D., Zhou, Jun, Smagin, Gennady N., Ryan, Donna H., Harris, Ruth B.S.·1997·Physiology & Behavior
DOI 10.1016/s0031-9384(96)00363-0
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Abstract

This study investigated whether paradoxical, or rapid eye movement (REM), sleep deprivation (SD) affected spatial memory. SD was induced in male Wistar rats by housing them on small platforms over water. They fell into the water if they lost muscle tone. Controls were either housed in tanks with large platforms (TC) or in normal cages (CC). All rats had free access to food and water. Each day they were tested in a place-learning set task using a Morris water maze. The rats were released from 6 different starting points (sets) and allowed 2 min to find a submerged platform. Two trials were conducted from each starting point. SD caused a significant decrement in performance in Trial 1 from Day 2. By Day 4, when distance swum to find the platform was plotted against set, area under the curve was doubled in SD compared to that in TC and CC rats, indicating a significant impairment in reference spatial memory. There was no consistent effect on working memory, indicated by Trial 2. SD caused weight loss and increased serum corticosterone compared to that in CC rats. There were no differences in concentrations of hypothalamic, hippocampal, or cortical catecholamines or their metabolites. Serotonin metabolism was elevated in the hypothalamus and hippocampus in SD rats. These results indicate that SD induced in rats housed on small platforms causes a substantial impairment of reference memory. The memory deficit may not be specific to SD because the rats are physically stressed and lose some nonREM sleep when housed in these conditions.

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  • In rodents, sleep deprivation by the platform (flower pot) method impairs spatial memory performance in the Morris water maze.

    “sleep deprivation by the platform (or flower pot) method leads to impaired performance of spatial memory in the Morris water maze,66”

    From Sleep Deprivation and Circadian Disruption (2015) · cites this paper as [66]

  • Sleep deprivation by the platform method impairs retention of passive avoidance memory, impairs spatial memory in the Morris water maze, and reduces long-term potentiation in the CA1 region of the hippocampus.

    “For example, sleep deprivation by the platform (or flowerpot) method resulted in impaired retention of passive avoidance memory, a context-dependent fear memory task,25 as well as impaired performance of spatial memory in the Morris water maze32 and a reduction in longterm potentiation in the CA1 region of the hippocampus.33”

    From Protective and damaging effects of stress mediators: central role of the brain (2006) · cites this paper as [32]

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Authors
Youngblood, B. D., Zhou, J., Smagin, G. N., Ryan, D. H., & Harris, R. B. S.
Affiliations
Pennington Biomedical Research Center (US)
Corresponding author
Harris, Ruth B.S.
ORCID
Venue
Physiology & Behavior
Publisher
Elsevier BV
Published
1997-02
Volume
61
Issue
2
Pages
249-256
ISSN
0031-9384 (ISSN-L)
DOI
10.1016/s0031-9384(96)00363-0
OpenAlex
W2027288004
Cited by
167
References
40
Citations by year
2026: 2 · 2025: 2 · 2024: 3 · 2023: 5 · 2022: 4 · 2021: 4 · 2020: 6 · 2019: 6 · 2018: 12 · 2017: 8 · 2016: 4 · 2015: 12 · 2014: 5 · 2013: 7 · 2012: 7
FWCI
1.36
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Physiology & Behavior (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Center for Research Resources (P20RR023491)
Topics
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Sleep and Wakefulness Research
  • Social Sciences › Psychology › Experimental and Cognitive Psychology › Sleep and Work-Related Fatigue
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
Keywords
sleep deprivation, REM sleep deprivation, spatial reference memory, place learning, Morris water maze, working memory, stress, corticosterone, weight loss, serotonin metabolism, catecholamines, hippocampus, hypothalamus
MeSH terms
Animals, Arousal, Catecholamines, Cerebral Cortex, Escape Reaction, Hippocampus, Hypothalamus, Male, Orientation, Mental Recall, Retention, Psychology, Serotonin, Sleep Deprivation, Sleep Stages, Sleep, REM, Rats, Wistar, Maze Learning, Rats

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