Activity of Porphyridium sp. polysaccharide against herpes simplex viruses in vitro and in vivo
Abstract
The cell wall sulfated polysaccharide of the red microalga Porphyridium sp. exhibited impressive antiviral activity against herpes simplex virus types 1 and 2 (HSV-1 and -2) both in vitro (cell culture) and in vivo (rats and rabbits). Depending on the concentration, this polysaccharide completely inhibited or slowed down the development of the cytopathic effect in HSV-infected cells, but did not show any cytotoxic effects on vero cells even when a concentration as high as 250 microg/ml was used. There was indirect evidence for a strong interaction between the polysaccharide and HSV and a weak interaction with the cell surface. When tested in vivo, Porphyridium sp. polysaccharide conferred significant and efficient protection against HSV-1 infection: at a concentration as low as 100 microg/ml, it prevented the appearance and development of symptoms of HSV-1 infection in rats and rabbits. The polysaccharide did not exhibit any cytotoxic effects at a concentration of 2 mg/ml in vivo.
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Algae are a good source of dietary fibers because of non-digestible polysaccharides in the algal cell wall, which possess antioxidant, hypoglycemic, antiviral, antitumor, and anti-inflammatory activities due to fucoidan and laminarin.
“Algae are considered as a good source of dietary fibers because of the presence of non-digestible polysaccharides in the algal cell wall (Figure 3) [11]. It has been shown that they possess activities, like antioxidant [12], hypoglycemic [13], antiviral [14,15], antitumor [16], and anti-inflammatory due to the presence of fucoidan and laminarin [17].”
From Overview on the Application of Modern Methods for the Extraction of Bioactive Compounds from Marine Macroalgae (2018) · cites this paper as [15]
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- Authors
- Huheihel, M., Ishanu, V., Tal, J., & Arad, S. (.
- Affiliations
- Ben-Gurion University of the Negev (IL)
- Corresponding author
- Huheihel, Mahmoud
- Venue
- Journal of Biochemical and Biophysical Methods
- Publisher
- Elsevier BV
- Published
- 2002-01
- Volume
- 50
- Issue
- 2-3
- Pages
- 189-200
- ISSN
- 0165-022X (ISSN-L)
- OpenAlex
- W1986624355
- Cited by
- 217
- References
- 24
- Citations by year
- 2026: 7 · 2025: 9 · 2024: 8 · 2023: 9 · 2022: 15 · 2021: 17 · 2020: 24 · 2019: 13 · 2018: 6 · 2017: 7 · 2016: 8 · 2015: 8 · 2014: 9 · 2013: 10 · 2012: 12
- FWCI
- 5.14
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Biochemical and Biophysical Methods (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Health Sciences › Medicine › Epidemiology › Herpesvirus Infections and Treatments
- Keywords
- Porphyridium, sulfated polysaccharides, cell wall polysaccharides, antiviral activity, herpes simplex virus type 1, herpes simplex virus type 2, red microalgae, cytopathic effect inhibition, cytotoxicity, cell surface binding
- MeSH terms
- Adsorption, Rhodophyta, Animals, Chlorocebus aethiops, Cytopathogenic Effect, Viral, Herpes Simplex, Male, Polysaccharides, Rabbits, Vero Cells, Keratitis, Herpetic, Herpesvirus 2, Human, Herpesvirus 1, Human, Rats, In Vitro Techniques
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