Overproduction of Acetyl-CoA Carboxylase Activity Increases the Rate of Fatty Acid Biosynthesis in Escherichia coli
Open accessAbstract
Acetyl-CoA carboxylase (ACC) catalyzes the first committed step of the fatty acid synthetic pathway. Although ACC has often been proposed to be a major rate-controlling enzyme of this pathway, no direct tests of this proposal in vivo have been reported. We have tested this proposal in Escherichia coli. The genes encoding the four subunits of E. coli ACC were cloned in a single plasmid under the control of a bacteriophage T7 promoter. Upon induction of gene expression, the four ACC subunits were overproduced in equimolar amounts. Overproduction of the proteins resulted in greatly increased ACC activity with a concomitant increase in the intracellular level of malonyl-CoA. The effects of ACC overexpression on the rate of fatty acid synthesis were examined in the presence of a thioesterase, which provided a metabolic sink for fatty acid overproduction. Under these conditions ACC overproduction resulted in a 6-fold increase in the rate of fatty acid synthesis.
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An increase in the activity levels of GPAT, PlsX and a fatty acyl-ACP thioesterase (tesA) overcomes this inhibition by detaching the fatty acid from the lipid biosynthesis.
“It appears that an increase in the activity levels of GPAT, PlsX and a fatty acyl-ACP thioesterase (tesA, EC 3.1.2.14) overcomes this inhibition by detaching the fatty acid from the lipid biosynthesis (Davis et al. 2000; Heath et al. 1994; Paoletti et al. 2007).”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Davis et al. 2000
Cites
[6] Use of T7 RNA polymerase to direct expression of cloned genes
Studier, F. W., Rosenberg, A. H., Dunn, J. J., & Dubendorff, J. W. (1990) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(90)85008-c · Cited by 6,562 · see citing works
Escherichia coli and Salmonella typhimurium: Cellular and molecular biology
Lejeune, P. (1988) · Biochimie · doi:10.1016/0300-9084(88)90119-8 · Cited by 4,086 · see citing works
(1993) · Methods in plant biochemistry · doi:10.1016/b978-0-12-461020-0.50001-9 · Cited by 1,774 · see citing works
Understanding the control of metabolism
(1997) · Choice Reviews Online · doi:10.5860/choice.34-6252 · Cited by 1,230 · see citing works
The Regulation of Cellular Systems
Heinrich, R., & Schuster, S. (1996) · Springer eBooks · doi:10.1007/978-1-4613-1161-4 · Cited by 1,224 · see citing works
Vector unpacking as a potential barrier for receptor-mediated polyplex gene delivery
Schaffer, D. V., Fidelman, N. A., Dan, N. R., & Lauffenburger, D. A. (2000) · Biotechnology and Bioengineering · doi:10.1002/(sici)1097-0290(20000305)67:5 · Cited by 525 · see citing works
The subcellular localization of acetyl-CoA carboxylase 2
Abu-Elheiga, L. A., Brinkley, W. R., Zhong, L., Chirala, S. S., Woldegiorgis, G., & Wakil, S. J. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.4.1444 · Cited by 450 · see citing works
(1990) · Methods in plant biochemistry · doi:10.1016/b978-0-12-461012-5.50001-x · Cited by 423 · see citing works
REGULATION OF MAMMALIAN ACETYL-COENZYME A CARBOXYLASE
Kim, K.-H. (1997) · Annual Review of Nutrition · doi:10.1146/annurev.nutr.17.1.77 · Cited by 394 · see citing works
Regulation of coenzyme A biosynthesis
Jackowski, S., & Rock, C. O. (1981) · Journal of Bacteriology · doi:10.1128/jb.148.3.926-932.1981 · Cited by 255 · see citing works
Millard, C. S., Chao, Y., Liao, J. C., et al. (1996) · Applied and Environmental Microbiology · doi:10.1128/aem.62.5.1808-1810.1996 · Cited by 245 · see citing works
The gene encoding the biotin carboxylase subunit of Escherichia coli acetyl-CoA carboxylase.
Li, S. J., & Cronan, J. E. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)48362-7 · Cited by 237 · see citing works
Self-citationRegulation of Fatty Acid Elongation and Initiation by Acyl-Acyl Carrier Protein in Escherichia coli
Heath, R. J., & Rock, C. O. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.4.1833 · Cited by 224 · see citing works
Heath, R. J., & Rock, C. O. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.18.10996 · Cited by 219 · see citing works
Acetyl Coenzyme A Carboxylase System of Escherichia coli
Guchhait, R. B., Polakis, S. E., Dimroth, P., et al. (1974) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)42203-5 · Cited by 209 · see citing works
Defective Export of a Periplasmic Enzyme Disrupts Regulation of Fatty Acid Synthesis
Cho, H., & Cronan, J. E. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.9.4216 · Cited by 172 · see citing works
Self-citationThe genes encoding the two carboxyltransferase subunits of Escherichia coli acetyl-CoA carboxylase.
Li, S. J., & Cronan, J. E. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)41860-1 · Cited by 148 · see citing works
Self-citationLi, S. J., & Cronan, J. E. (1993) · Journal of Bacteriology · doi:10.1128/jb.175.2.332-340.1993 · Cited by 148 · see citing works
Self-citationJiang, P., & Cronan, J. E. (1994) · Journal of Bacteriology · doi:10.1128/jb.176.10.2814-2821.1994 · Cited by 140 · see citing works
Self-citationChapman‐Smith, A., Turner, D., Cronan, J. E., et al. (1994) · Biochemical Journal · doi:10.1042/bj3020881 · Cited by 139 · see citing works
Self-citationThe nucleotide sequence and characterization of the relA gene of Escherichia coli.
Metzger, S., Dror, I., Aizenman, E., Schreiber, G., Toone, M., Friesen, J. D., Cashel, M., & Glaser, G. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)37644-6 · Cited by 133 · see citing works
Characterization of rate-controlling steps in vivo by use of an adjustable expression vector.
Walsh, K., & Koshland, D. E. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.11.3577 · Cited by 132 · see citing works
Heath, R. J., Jackowski, S., & Rock, C. O. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)47234-1 · Cited by 123 · see citing works
Acetyl-CoA carboxylase exerts strong flux control over lipid synthesis in plants
Page, R., Okada, S., & Harwood, J. L. (1994) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(94)90242-9 · Cited by 115 · see citing works
Pausing and termination by bacteriophage T7 RNA polymerase
Lyakhov, D. L., He, B., Zhang, X., Studier, F. W., Dunn, J. J., & McAllister, W. T. (1998) · Journal of Molecular Biology · doi:10.1006/jmbi.1998.1854 · Cited by 108 · see citing works
Acetyl Coenzyme A Carboxylase System of Escherichia coli
Polakis, S. E., Guchhait, R. B., Zwergel, E. E., et al. (1974) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)42205-9 · Cited by 100 · see citing works
The regulation of Acetyl-CoA carboxylase—a potential target for the action of hypolipidemic agents
Munday, M. R., & Hemingway, C. J. (1999) · Advances in Enzyme Regulation · doi:10.1016/s0065-2571(98)00016-8 · Cited by 99 · see citing works
Mutant of Escherichia coli Deficient in the Synthesis of cis -Vaccenic Acid
Gelmann, E. P., & Cronan, J. E. (1972) · Journal of Bacteriology · doi:10.1128/jb.112.1.381-387.1972 · Cited by 97 · see citing works
Self-citationStringent Control of Fatty Acid Synthesis in Escherichia coli
Polakis, S. E., Guchhait, R. B., & Lane, M. D. (1973) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)43280-8 · Cited by 96 · see citing works
Subrahmanyam, S., & Cronan, J. E. (1998) · Journal of Bacteriology · doi:10.1128/jb.180.17.4596-4602.1998 · Cited by 90 · see citing works
Self-citationHeath, R. J., & Rock, C. O. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.26.15531 · Cited by 88 · see citing works
Studies on the mechanism of fatty acid synthesis
Joshi, V. C., & Wakil, S. J. (1971) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(71)90234-7 · Cited by 83 · see citing works
Alberts, A. W., & Vagelos, P. R. (1972) · The Enzymes · doi:10.1016/s1874-6047(08)60037-2 · Cited by 66 · see citing works
Harder, M. E., Beacham, I. R., Cronan, J. E., et al. (1972) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.69.11.3105 · Cited by 66 · see citing works
Self-citationBlanchard, C. Z., & Waldrop, G. L. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.30.19140 · Cited by 50 · see citing works
Jeng, S., Gardner, J. F., & Gumport, R. I. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)41775-9 · Cited by 38 · see citing works
Expression analysis of cloned chromosomal segments of Escherichia coli
Sankar, P., Hutton, M. E., VanBogelen, R. A., et al. (1993) · Journal of Bacteriology · doi:10.1128/jb.175.16.5145-5152.1993 · Cited by 29 · see citing works
Li, S. J., Rock, C. O., & Cronan, J. E. (1992) · Journal of Bacteriology · doi:10.1128/jb.174.17.5755-5757.1992 · Cited by 27 · see citing works
Self-citationStabilization of an acetyl-coenzyme a carboxylase complex from Pseudomonas citronellolis
Fall, R. (1976) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(76)90022-9 · Cited by 26 · see citing works
Silbert, D. F., Pohlman, T. H., & Chapman, A. E. (1976) · Journal of Bacteriology · doi:10.1128/jb.126.3.1351-1354.1976 · Cited by 24 · see citing works
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Campbell, J. W., & Cronan, J. E. (2001) · Annual Review of Microbiology · doi:10.1146/annurev.micro.55.1.305 · Cited by 479 · see citing works
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- Authors
- Davis, M. S., Solbiati, J., & Cronan, J. E.
- Affiliations
- University of Illinois Urbana-Champaign (US)
- Corresponding author
- Cronan, John E.
- ORCID
- Cronan, John E.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2000-09
- Volume
- 275
- Issue
- 37
- Pages
- 28593-28598
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1993899465
- Cited by
- 479
- References
- 41
- Citations by year
- 2026: 11 · 2025: 22 · 2024: 24 · 2023: 15 · 2022: 20 · 2021: 22 · 2020: 26 · 2019: 27 · 2018: 32 · 2017: 35 · 2016: 34 · 2015: 27 · 2014: 38 · 2013: 34 · 2012: 21
- FWCI
- 3.29
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Lipids, and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- acetyl-CoA carboxylase, fatty acid synthesis, Escherichia coli, enzyme overexpression, malonyl-CoA, thioesterase
- MeSH terms
- Acetyl-CoA Carboxylase, Escherichia coli, Fatty Acids
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