The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Virus-Inspired Membrane Encapsulation of DNA Nanostructures To Achieve In Vivo Stability
Open accessAbstract
DNA nanotechnology enables engineering of molecular-scale devices with exquisite control over geometry and site-specific functionalization. This capability promises compelling advantages in advancing nanomedicine; nevertheless, instability in biological environments and innate immune activation remain as obstacles for in vivo application. Natural particle systems (i.e., viruses) have evolved mechanisms to maintain structural integrity and avoid immune recognition during infection, including encapsulation of their genome and protein capsid shell in a lipid envelope. Here we introduce virus-inspired enveloped DNA nanostructures as a design strategy for biomedical applications. Achieving a high yield of tightly wrapped unilamellar nanostructures, mimicking the morphology of enveloped virus particles, required precise control over the density of attached lipid conjugates and was achieved at 1 per ∼180 nm(2). Envelopment of DNA nanostructures in PEGylated lipid bilayers conferred protection against nuclease digestion. Immune activation was decreased 2 orders of magnitude below controls, and pharmacokinetic bioavailability improved by a factor of 17. By establishing a design strategy suitable for biomedical applications, we have provided a platform for the engineering of sophisticated, translation-ready DNA nanodevices.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors
Kawai, T., & Akira, S. (2010) · Nature Immunology · doi:10.1038/ni.1863 · Cited by 9,304
Folding DNA to create nanoscale shapes and patterns
Rothemund, P. W. K. (2006) · Nature · doi:10.1038/nature04586 · Cited by 7,354
Renal clearance of quantum dots
Soo Choi, H., Liu, W., Misra, P., Tanaka, E., Zimmer, J. P., Itty Ipe, B., Bawendi, M. G., & Frangioni, J. V. (2007) · Nature Biotechnology · doi:10.1038/nbt1340 · Cited by 4,476
Self-assembly of DNA into nanoscale three-dimensional shapes
Douglas, S. M., Dietz, H., Liedl, T., Högberg, B., Graf, F., & Shih, W. M. (2009) · Nature · doi:10.1038/nature08016 · Cited by 2,571
Self-citationA Logic-Gated Nanorobot for Targeted Transport of Molecular Payloads
Douglas, S. M., Bachelet, I., & Church, G. M. (2012) · Science · doi:10.1126/science.1214081 · Cited by 2,180
Amphipathic polyethyleneglycols effectively prolong the circulation time of liposomes
Klibanov, A. L., Maruyama, K., Torchilin, V. P., & Huang, L. (1990) · FEBS Letters · doi:10.1016/0014-5793(90)81016-h · Cited by 2,027
Mediating Tumor Targeting Efficiency of Nanoparticles Through Design
Perrault, S. D., Walkey, C., Jennings, T., Fischer, H. C., & Chan, W. C. W. (2009) · Nano Letters · doi:10.1021/nl900031y · Cited by 1,513
Self-citationÅkerman, M. E., Chan, W. C. W., Laakkonen, P., Bhatia, S. N., & Ruoslahti, E. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.152463399 · Cited by 1,437
Folding DNA into Twisted and Curved Nanoscale Shapes
Dietz, H., Douglas, S. M., & Shih, W. M. (2009) · Science · doi:10.1126/science.1174251 · Cited by 1,335
Self-citationThree-Dimensional Structures Self-Assembled from DNA Bricks
Ke, Y., Ong, L. L., Shih, W. M., & Yin, P. (2012) · Science · doi:10.1126/science.1227268 · Cited by 1,246
Self-citationRapid prototyping of 3D DNA-origami shapes with caDNAno
Douglas, S. M., Marblestone, A. H., Teerapittayanon, S., Vazquez, A., Church, G. M., & Shih, W. M. (2009) · Nucleic Acids Research · doi:10.1093/nar/gkp436 · Cited by 1,224
Self-citationMolecularly self-assembled nucleic acid nanoparticles for targeted in vivo siRNA delivery
Lee, H., Lytton-Jean, A. K. R., Chen, Y., Love, K. T., Park, A. I., Karagiannis, E. D., Sehgal, A., Querbes, W., Zurenko, C. S., Jayaraman, M., et al. (2012) · Nature Nanotechnology · doi:10.1038/nnano.2012.73 · Cited by 1,169
Seeman, N. C. (2010) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060308-102244 · Cited by 992
Minimal "Self" Peptides That Inhibit Phagocytic Clearance and Enhance Delivery of Nanoparticles
Rodriguez, P. L., Harada, T., Christian, D. A., Pantano, D. A., Tsai, R. K., & Discher, D. E. (2013) · Science · doi:10.1126/science.1229568 · Cited by 985
Nanoparticle-Aptamer Bioconjugates
Farokhzad, O. C., Jon, S., Khademhosseini, A., Tran, T.-N. T., LaVan, D. A., & Langer, R. (2004) · Cancer Research · doi:10.1158/0008-5472.can-04-2550 · Cited by 897
Synthetic Lipid Membrane Channels Formed by Designed DNA Nanostructures
Langecker, M., Arnaut, V., Martin, T. G., List, J., Renner, S., Mayer, M., Dietz, H., & Simmel, F. C. (2012) · Science · doi:10.1126/science.1225624 · Cited by 758
Li, J., Pei, H., Zhu, B., Liang, L., Wei, M., He, Y., Chen, N., Li, D., Huang, Q., & Fan, C. (2011) · ACS Nano · doi:10.1021/nn202774x · Cited by 748
Action of Saponin on Biological Cell Membranes
BANGHAM, A. D., & HORNE, R. W. (1962) · Nature · doi:10.1038/196952a0 · Cited by 508
Nanoparticles that communicate in vivo to amplify tumour targeting
Park, J., Lin, K., Singh, N., Schwöppe, C., Mesters, R. M., Berdel, W. E., Ruoslahti, E. I., Sailor, M. J., von Maltzhan, G., & Bhatia, S. N. (2010) · DSpace@MIT (Massachusetts Institute of Technology) · Cited by 500
Cellular Immunostimulation by CpG-Sequence-Coated DNA Origami Structures
Schüller, V. J., Heidegger, S., Sandholzer, N., Nickels, P. C., Suhartha, N. A., Endres, S., Bourquin, C., & Liedl, T. (2011) · ACS Nano · doi:10.1021/nn203161y · Cited by 495
DNA-nanotube-induced alignment of membrane proteins for NMR structure determination
Douglas, S. M., Chou, J. J., & Shih, W. M. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0700930104 · Cited by 480
Self-citationStability of DNA Origami Nanoarrays in Cell Lysate
Mei, Q., Wei, X., Su, F., Liu, Y., Youngbull, C., Johnson, R., Lindsay, S., Yan, H., & Meldrum, D. (2011) · Nano Letters · doi:10.1021/nl1040836 · Cited by 338
Six-Helix Bundles Designed from DNA
Mathieu, F., Liao, S., Kopatsch, J., Wang, T., Mao, C., & Seeman, N. C. (2005) · Nano Letters · doi:10.1021/nl050084f · Cited by 315
Cooperative nanomaterial system to sensitize, target, and treat tumors
Park, J.-H., von Maltzahn, G., Xu, M. J., Fogal, V., Kotamraju, V. R., Ruoslahti, E., Bhatia, S. N., & Sailor, M. J. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0909565107 · Cited by 307
pH-Sensitive liposomes-principle and application in cancer therapy
Karanth, H., & Murthy, R. S. R. (2007) · Journal of Pharmacy and Pharmacology · doi:10.1211/jpp.59.4.0001 · Cited by 294
Effects of linker sequences on vesicle fusion mediated by lipid-anchored DNA oligonucleotides
Chan, Y.-H. M., van Lengerich, B., & Boxer, S. G. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0812356106 · Cited by 282
Action of Saponin on Biological Cell Membranes
GLAUERT, A. M., DINGLE, J. T., & LUCY, J. A. (1962) · Nature · doi:10.1038/196953a0 · Cited by 259
Nadano, D., Yasuda, T., & Kishi, K. (1993) · Clinical Chemistry · doi:10.1093/clinchem/39.3.448 · Cited by 236
In vivo assembly of nanoparticle components to improve targeted cancer imaging
Perrault, S. D., & Chan, W. C. W. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1001367107 · Cited by 181
Self-citationLight‐Driven Conformational Switch of i‐Motif DNA
Liu, H., Xu, Y., Li, F., Yang, Y., Wang, W., Song, Y., & Liu, D. (2007) · Angewandte Chemie International Edition · doi:10.1002/anie.200604589 · Cited by 169
Autonomous molecular cascades for evaluation of cell surfaces
Rudchenko, M., Taylor, S., Pallavi, P., Dechkovskaia, A., Khan, S., Butler Jr, V. P., Rudchenko, S., & Stojanovic, M. N. (2013) · Nature Nanotechnology · doi:10.1038/nnano.2013.142 · Cited by 166
Purification of DNA-origami nanostructures by rate-zonal centrifugation
Lin, C., Perrault, S. D., Kwak, M., Graf, F., & Shih, W. M. (2013) · Nucleic Acids Research · doi:10.1093/nar/gks1070 · Cited by 160
Self-citationJuul, S., Iacovelli, F., Falconi, M., Kragh, S. L., Christensen, B., Frøhlich, R. F., Franch, O., Kristoffersen, E. L., Stougaard, M., Leong, K. W., et al. (2013) · ACS Nano · doi:10.1021/nn4030543 · Cited by 159
Native Morphology of Influenza Virions
Noda, T. (2012) · Frontiers in Microbiology · doi:10.3389/fmicb.2011.00269 · Cited by 109
Synthetic Lipid Membrane Channels formed by Designed DNA Nanostructures
Langecker, M., Arnaut, V., Martin, T. G., List, J., Renner, S., Mayer, M., Dietz, H., & Simmel, F. C. (2013) · Biophysical Journal · doi:10.1016/j.bpj.2012.11.3022 · Cited by 108
Negatively Stained Lipoprotein Membranes
Horne, R. W., Bangham, A. D., & Whittaker, V. P. (1963) · Nature · doi:10.1038/2001340a0 · Cited by 65
Cited by
The current state and future directions of RNAi-based therapeutics
Setten, R. L., Rossi, J. J., & Han, S.-P. (2019) · Nature Reviews Drug Discovery · doi:10.1038/s41573-019-0017-4 · Cited by 1,443
DNA nanotechnology from the test tube to the cell
Chen, Y.-J., Groves, B., Muscat, R. A., & Seelig, G. (2015) · Nature Nanotechnology · doi:10.1038/nnano.2015.195 · Cited by 594
Ponnuswamy, N., Bastings, M. M. C., Nathwani, B., Ryu, J. H., Chou, L. Y. T., Vinther, M., Li, W. A., Anastassacos, F. M., Mooney, D. J., & Shih, W. M. (2017) · Nature Communications · doi:10.1038/ncomms15654 · Cited by 508
Self-citationNuclease resistance of DNA nanostructures
Chandrasekaran, A. R. (2021) · Nature Reviews Chemistry · doi:10.1038/s41570-021-00251-y · Cited by 382
Nanocarrier imaging at single-cell resolution across entire mouse bodies with deep learning
Luo, J., Molbay, M., Chen, Y., Horvath, I., Kadletz, K., Kick, B., Zhao, S., Al-Maskari, R., Singh, I., Ali, M., et al. (2025) · Nature Biotechnology · doi:10.1038/s41587-024-02528-1 · Cited by 53
Metadata
- Authors
- Perrault, S. D., & Shih, W. M.
- Venue
- ACS Nano
- Publisher
- American Chemical Society (ACS)
- Published
- 2014-04-22
- Volume
- 8
- Issue
- 5
- Pages
- 5132-5140
- ISSN
- 1936-0851 (ISSN-L)
- 1936-086X
- OpenAlex
- W1964535590
- Cited by
- 522
- References
- 36
- FWCI
- 13.14
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
