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Cited by 165Gastrointestinal motility and disordersInflammatory Bowel DiseaseGut microbiota and health

T-Cell Activation in Patients With Irritable Bowel Syndrome

Öhman, Lena, Isaksson, Stefan, Lindmark, Ann-Charlotte, Posserud, Iris, Stotzer, P-O, Strid, Hans, Sjövall, Henrik, Simrén, Magnus·2009·The American Journal of Gastroenterology
DOI 10.1038/ajg.2009.116
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Abstract

OBJECTIVES: Irritable bowel syndrome (IBS) has been found to be associated with low-grade immune activation in a subset of patients. We therefore investigated blood and colonic T-cell activity in IBS patients. METHODS: Blood samples were initially obtained from 74 IBS patients and 30 controls. Supplementary blood samples, to confirm data, were taken from another cohort (26 patients and 14 controls). In addition, colonic biopsies were taken from a third cohort (11 patients and 10 controls). Peripheral blood and colonic mononuclear cells were stimulated with anti-CD3/CD28 antibodies. Proliferation, cytokine secretion, and T-cell phenotype were investigated. IBS symptom severity was assessed. RESULTS: IBS patients displayed an activated phenotype with increased frequencies of blood T cells expressing CD69 and integrin beta7/HLA-DR. Anti-CD3/CD28-stimulated blood and colonic T cells from IBS patients proliferated less than T cells from controls. IBS patients had an increased polyclonally stimulated T-cell secretion of IL-1beta, which also weakly correlated with increased bowel habit dissatisfaction. Furthermore, despite normal frequencies of CD25high T cells in the blood of IBS patients, lower blood CD25high T-cell frequencies were modestly correlated with more bowel habit dissatisfaction and increased total IBS symptom severity. CONCLUSIONS: IBS patients have an increased frequency of activated T cells, demonstrated by the expression of activation markers and reduced proliferation in response to restimulation in vitro. The increased level of T-cell activation is consistent with the hypothesis of low-grade immune activation in IBS and may also be involved in symptom generation in IBS.

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Crossref · 10.1038/ajg.2009.116

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  • Patients with IBS may have increased numbers of activated T cells in the colon and peripheral blood compared with controls.

    “In addition to elevated concentrations of T cells, patients with IBS may also have an increased numbers of activated T cells in the colon (36) and peripheral blood (122) compared with controls.”

    From Intestinal microbiota and immune function in the pathogenesis of irritable bowel syndrome (2013) · cites this paper as [122]

  • Increases in the secretion of TNF-α following lipopolysaccharide stimulation of peripheral blood mononuclear cells and of IL-1β following T-cell anti-CD3/CD28 antibody stimulation were significantly associated with anxiety and bowel habit dissatisfaction and overall IBS symptoms, respectively.

    “Furthermore, increases in the secretion of TNF-α following LPS stimulation of PBMCs and of IL-1β following T-cell anti-CD3/CD28 antibodies stimulation were significantly associated with anxiety (98) and bowel habit dissatisfaction and overall IBS symptoms (122), respectively.”

    From Intestinal microbiota and immune function in the pathogenesis of irritable bowel syndrome (2013) · cites this paper as [122]

  • Peripheral blood T cells from patients with IBS express elevated levels of integrin β7.

    “Peripheral blood T cells from patients with IBS express elevated levels of integrin β7 (122, 123), which is important for homing of cells to the intestines.”

    From Intestinal microbiota and immune function in the pathogenesis of irritable bowel syndrome (2013) · cites this paper as [122]

  • Cytokine secretion following in vitro stimulation of peripheral blood lymphocytes/monocytes may also be altered in patients with IBS.

    “Cytokine secretion following in vitro stimulation of peripheral blood lymphocytes/monocytes may also be altered in patients with IBS (90, 98, 122).”

    From Intestinal microbiota and immune function in the pathogenesis of irritable bowel syndrome (2013) · cites this paper as [122]

  • Overall, alterations in cytokine secretion by stimulated peripheral blood cells appear to favor an activated immune state and an altered immune response shifted toward a T-helper 2 phenotype in IBS patients.

    “Overall, it seems that alterations in cytokine secretion by stimulated peripheral blood cells appear to favor an activated immune state (98, 122), and an altered immune response shifted toward a T-helper 2 phenotype(90) in IBS patients.”

    From Intestinal microbiota and immune function in the pathogenesis of irritable bowel syndrome (2013) · cites this paper as [122]

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Authors
Öhman, L., Isaksson, S., Lindmark, A.-C., Posserud, I., Stotzer, P.-O., Strid, H., Sjövall, H., & Simrén, M.
Affiliations
University of Gothenburg (SE)
Corresponding author
Öhman, Lena
ORCID
Venue
The American Journal of Gastroenterology
Publisher
Ovid Technologies (Wolters Kluwer Health)
Published
2009-04-14
Volume
104
Issue
5
Pages
1205-1212
ISSN
  • 0002-9270 (ISSN-L)
  • 1572-0241
DOI
10.1038/ajg.2009.116
OpenAlex
W2069870942
Cited by
165
References
32
Citations by year
2026: 3 · 2025: 1 · 2024: 2 · 2023: 3 · 2022: 7 · 2021: 7 · 2020: 4 · 2019: 6 · 2018: 13 · 2017: 10 · 2016: 16 · 2015: 14 · 2014: 10 · 2013: 12 · 2012: 21
FWCI
20.18
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (unspecified)
Locations
  • The American Journal of Gastroenterology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Inflammatory Bowel Disease
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Keywords
irritable bowel syndrome, T cell activation, CD69, beta7 integrin, HLA-DR, T cell proliferation, IL-1β, IBS symptom severity
MeSH terms
Adult, Biopsy, Needle, Cells, Cultured, Female, Flow Cytometry, Humans, Intestinal Mucosa, Lymphocyte Activation, Male, Middle Aged, Probability, Reference Values, Sensitivity and Specificity, Severity of Illness Index, T-Lymphocytes, Case-Control Studies, Cytokines, Statistics, Nonparametric, Irritable Bowel Syndrome, Cell Proliferation, Interleukin-1beta, Young Adult

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