B‐cell activation in patients with irritable bowel syndrome (IBS)
Abstract
Abstract Patients with irritable bowel syndrome (IBS) may have a low grade immune activation. However, little is known about the properties of B cells of IBS patients. We therefore investigated activation level and antigen presenting phenotype of blood B cells of IBS patients. We also examined B‐cell responses to lipopolysaccharide (LPS) and probiotic bacteria. Blood samples were obtained from 74 IBS patients and 30 healthy subjects. Peripheral blood mononuclear cells were isolated and stimulated with LPS or an UV‐light inactivated bacterial cocktail consisting of the probiotic Gram‐positive strains; Lactobacillus paracasei ssp. paracasei 19, Lactobacillus acidophilus La5, Bifidobacterium lactis B612. The phenotype of CD19+ B cells was investigated by flow cytometry before and after 72 h cell culture. Furthermore, IBS symptom severity was assessed. B cells isolated from blood of IBS patients displayed an amplified activation level as demonstrated by increased cell surface expression of IgG, and also the costimulatory molecules CD80 and CD86. Expression of antigen presenting HLA‐DR and costimulatory molecule CD40 on B cells was, however comparable in IBS patients and controls. B cells of IBS patients displayed an impaired ability to increase expression of CD80, but not CD86, in response to both LPS as well as probiotic bacteria stimulations. To conclude, blood B cells of IBS patients have an increased activation level. Bacterial component induced expression of the costimulatory molecule CD80, regarded as important for tolerance induction, is impaired. These data suggest that B‐cell antigen presentation in IBS patients is associated with altered capacity of providing costimulation to T cells.
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Patients with IBS may have increased expression of IgG and the costimulatory molecules CD80 and CD86 on B cells and an increased frequency of peripheral blood IgG+CD80+ and IgG+CD86+ B cells.
“In addition, patients with IBS may have increased expression of IgG and the costimulatory molecules CD80 and CD86 on B cells and an increased frequency of peripheral blood IgG+CD80+ and IgG+CD86+ B cells (124).”
From Intestinal microbiota and immune function in the pathogenesis of irritable bowel syndrome (2013) · cites this paper as [124]
B cells from patients with IBS had an impaired ability to upregulate CD80 in response to in vitro lipopolysaccharide stimulation, suggesting that B cells from patients with IBS may have an altered ability to costimulate and regulate T cells.
“Furthermore, B cells from patients with IBS had an impaired ability to upregulate CD80 in response to in vitro lipopolysaccharide (LPS) stimulation, suggesting that B cells from patients with IBS may have an altered ability to costimulate and regulate T cells.”
From Intestinal microbiota and immune function in the pathogenesis of irritable bowel syndrome (2013) · cites this paper as [124]
In patients with PI-IBS, activation of the GI immune system follows acute GI infection.
“The importance of the interaction between the intestinal microbiota and immune system in IBS patients is demonstrated by a few studies in patients with PI-IBS, indicating activation of the GI immune system following the acute GI infection (57, 58, 75, 89, 94, 124, 168, 170, 187)”
From Intestinal microbiota and immune function in the pathogenesis of irritable bowel syndrome (2013) · cites this paper as [124]
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- Authors
- öhman, L., Lindmark, A. ‐., Isaksson, S., Posserud, I., Strid, H., Sjövall, H., & Simrén, M.
- Affiliations
- University of Gothenburg (SE)
- Corresponding author
- öhman, L.
- Venue
- Neurogastroenterology & Motility
- Publisher
- Wiley
- Published
- 2009-05-20
- Volume
- 21
- Issue
- 6
- Pages
- 644
- ISSN
- 1350-1925 (ISSN-L)
- 1365-2982
- OpenAlex
- W1972949213
- Cited by
- 78
- References
- 30
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 2 · 2020: 3 · 2019: 5 · 2018: 4 · 2017: 3 · 2016: 8 · 2015: 2 · 2014: 4 · 2013: 10 · 2012: 13
- FWCI
- 10.62
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neurogastroenterology & Motility (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Inflammatory Bowel Disease
- Keywords
- irritable bowel syndrome, B cell activation, B cell antigen presentation, CD80, CD86, costimulation, lipopolysaccharide, probiotic bacteria, Lactobacillus paracasei, Lactobacillus acidophilus, Bifidobacterium animalis subsp. lactis, HLA-DR, IgG, immune activation, T cell tolerance
- MeSH terms
- Adult, Antigen-Presenting Cells, B-Lymphocytes, Cells, Cultured, Female, Flow Cytometry, Gram-Positive Bacteria, HLA-DR Antigens, Humans, Immunoglobulin G, Leukocyte Count, Lymphocyte Activation, Male, Middle Aged, Phenotype, Stimulation, Chemical, B7-1 Antigen, CD40 Antigens, Probiotics, Integrin beta Chains, Irritable Bowel Syndrome, B7-2 Antigen, Young Adult
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