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Structural segregation of gut microbiota between colorectal cancer patients and healthy volunteers
Open accessAbstract
Despite a long-suspected role in the development of human colorectal cancer (CRC), the composition of gut microbiota in CRC patients has not been adequately described. In this study, fecal bacterial diversity in CRC patients (n=46) and healthy volunteers (n=56) were profiled by 454 pyrosequencing of the V3 region of the 16S ribosomal RNA gene. Both principal component analysis and UniFrac analysis showed structural segregation between the two populations. Forty-eight operational taxonomic units (OTUs) were identified by redundancy analysis as key variables significantly associated with the structural difference. One OTU closely related to Bacteroides fragilis was enriched in the gut microbiota of CRC patients, whereas three OTUs related to Bacteroides vulgatus and Bacteroides uniformis were enriched in that of healthy volunteers. A total of 11 OTUs belonging to the genera Enterococcus, Escherichia/Shigella, Klebsiella, Streptococcus and Peptostreptococcus were significantly more abundant in the gut microbiota of CRC patients, and 5 OTUs belonging to the genus Roseburia and other butyrate-producing bacteria of the family Lachnospiraceae were less abundant. Real-time quantitative PCR further validated the significant reduction of butyrate-producing bacteria in the gut microbiota of CRC patients by measuring the copy numbers of butyryl-coenzyme A CoA transferase genes (Mann-Whitney test, P<0.01). Reduction of butyrate producers and increase of opportunistic pathogens may constitute a major structural imbalance of gut microbiota in CRC patients.
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“However, ETBF was reported to be enriched in fecal samples of patients with CRC compared with controls.35, 44, 45”
From Influence of the Gut Microbiome, Diet, and Environment on Risk of Colorectal Cancer (2020) · cites this paper as [45]
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“several studies have shown that compared with controls, patients with CRC had lower abundances of Bifidobacterium and Lactobacillus spp,16, 18 and other SCFA-producing bacteria, such as the genera Clostridium and Roseburia, the family Lachnospiraceae, and Faecalibacterium prausnitzii.15, 35, 37, 45, 53, 129”
From Influence of the Gut Microbiome, Diet, and Environment on Risk of Colorectal Cancer (2020) · cites this paper as [45]
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Metadata
- Authors
- Wang, T., Cai, G., Qiu, Y., Fei, N., Zhang, M., Pang, X., Jia, W., Cai, S., & Zhao, L.
- Affiliations
- Shanghai Jiao Tong University (CN)
- Shanghai Medical College of Fudan University (CN)
- University of North Carolina at Greensboro (US)
- Corresponding author
- Zhao, Liping
- ORCID
- Venue
- The ISME Journal
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-08-18
- Volume
- 6
- Issue
- 2
- Pages
- 320-329
- ISSN
- 1751-7362 (ISSN-L)
- 1751-7370
- PMC
- PMC3260502
- OpenAlex
- W2015567271
- Cited by
- 1,287
- References
- 65
- Citations by year
- 2026: 49 · 2025: 80 · 2024: 126 · 2023: 118 · 2022: 139 · 2021: 155 · 2020: 134 · 2019: 106 · 2018: 101 · 2017: 80 · 2016: 53 · 2015: 46 · 2014: 38 · 2013: 39 · 2012: 19
- FWCI
- 12.71
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The ISME Journal (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (30730005)
- National Science and Technology Major Project
- National Key Research and Development Program of China
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Infectious Diseases › Clostridium difficile and Clostridium perfringens research
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Keywords
- colorectal cancer, gut microbiota, microbial community structure, Bacteroides fragilis, Bacteroides vulgatus, Bacteroides uniformis, butyrate-producing bacteria, Lachnospiraceae, Roseburia, opportunistic pathogens, Enterococcus, Escherichia-Shigella, Klebsiella, Streptococcus, Peptostreptococcus, dysbiosis
- MeSH terms
- Acyl Coenzyme A, Adult, Aged, Bacteria, Feces, Female, Humans, Male, Middle Aged, RNA, Ribosomal, 16S, Colorectal Neoplasms, Reproducibility of Results, Coenzyme A-Transferases, Bacterial Physiological Phenomena, Principal Component Analysis, Gastrointestinal Tract, Biodiversity, Metagenome
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