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Cited by 347

The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.

Integrase-mediated spacer acquisition during CRISPR–Cas adaptive immunity

Nuñez, James K., Lee, Amy S. Y., Engelman, Alan, Doudna, Jennifer A.·2015·Nature
DOI 10.1038/nature14237PMC PMC4359072
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Crossref · 10.1038/nature14237

Cites

7 of 7 references verified0 citation errors · 0 not checkable

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  • 3′-OH groups of the protospacer intermediate catalyse 2 sequential nucleophilic attacks at both 5′ ends of the first repeat of the CRISPR array.

    “3′-OH groups of the protospacer intermediate catalyse 2 sequential nucleophilic attacks at both 5′ ends of the first repeat of the CRISPR array [28].”

    From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [28]

    In “Mechanism of protospacer integration” (page not provided), the paper proposes sequential attacks by the two protospacer 3′-OH ends on opposite strands at the repeat borders. The citing sentence presents both attacks at the first repeat as established, whereas the paper says its in vitro system predominantly traps the first step and observes integration at every repeat, with the first preferred. (LLM, conf=0.84)

    Experimental — accuracy not yet measured

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Citations

Cites

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Research works printed but not linked: 42

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  9. Brouns, S. J. et al. Small CRISPR RNAs guide antiviral defense in prokaryotes. Science 321, 960–964 (2008) · DOI · Paper page
  10. Garneau, J. E. et al. The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA. Nature 468, 67–71 (2010) · DOI
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  13. Swarts, D. C., Mosterd, C., van Passel, M. W. & Brouns, S. J. CRISPR interference directs strand specific spacer acquisition. PLoS ONE 7, e35888 (2012) · DOI
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  15. Wiedenheft, B. et al. Structural basis for DNase activity of a conserved protein implicated in CRISPR-mediated genome defense. Structure 17, 904–912 (2009) · DOI · Paper page
  16. Babu, M. et al. A dual function of the CRISPR–Cas system in bacterial antivirus immunity and DNA repair. Mol. Microbiol. 79, 484–502 (2011) · DOI · Paper page
  17. Kim, T. Y., Shin, M., Huynh Thi Yen, L. & Kim, J. S. Crystal structure of Cas1 from Archaeoglobus fulgidus and characterization of its nucleolytic activity. Biochem. Biophys. Res. Commun. 441, 720–725 (2013) · DOI
  18. Beloglazova, N. et al. A novel family of sequence-specific endoribonucleases associated with the clustered regularly interspaced short palindromic repeats. J. Biol. Chem. 283, 20361–20371 (2008) · DOI
  19. Samai, P., Smith, P. & Shuman, S. Structure of a CRISPR-associated protein Cas2 from Desulfovibrio vulgaris. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66, 1552–1556 (2010) · DOI
  20. Nam, K. H. et al. Double-stranded endonuclease activity in Bacillus halodurans clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas2 protein. J. Biol. Chem. 287, 35943–35952 (2012) · DOI
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Metadata

Authors
Nuñez, J. K., Lee, A. S. Y., Engelman, A., & Doudna, J. A.
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
2015-02-18
Volume
519
Issue
7542
Pages
193-198
ISSN
  • 0028-0836
  • 1476-4687
DOI
10.1038/nature14237
PMC
PMC4359072
Cited by
347
References
7
Language
en
Licence

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