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Pervasive generation of oppositely oriented spacers during CRISPR adaptation

Shmakov, Sergey, Savitskaya, Ekaterina, Semenova, Ekaterina, Logacheva, Maria D., Datsenko, Kirill A., Severinov, Konstantin·2014·Nucleic Acids Research
DOI 10.1093/nar/gku226PMC PMC4027179
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  • Spacers are arranged chronologically within the CRISPR array, with a few exceptions.

    “Therefore, spacers are arranged chronologically within the array, with a few exceptions [29,30].”

    From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [29]

    The Results section describes newly acquired spacers and their orientation, but does not address whether spacers are arranged chronologically within an array or whether that ordering has exceptions. Searches for chronological order, acquisition order, and leader-end insertion found no passage establishing the claim. (LLM, conf=0.75)

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Research works printed but not linked: 38

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Metadata

Authors
Shmakov, S., Savitskaya, E., Semenova, E., Logacheva, M. D., Datsenko, K. A., & Severinov, K.
Venue
Nucleic Acids Research
Publisher
Oxford University Press (OUP)
Published
2014-04-11
Volume
42
Issue
9
Pages
5907-5916
ISSN
  • 1362-4962
  • 0305-1048
DOI
10.1093/nar/gku226
PMC
PMC4027179
Cited by
70
References
0
Language
en
Licence
creativecommons.org/licenses/by-nc/3.0/ (vor, 907 d)

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