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Cited by 624Neuroscience and Neuropharmacology ResearchMemory and Neural MechanismsMotor Control and Adaptation

Labelling and optical erasure of synaptic memory traces in the motor cortex

Open access
Hayashi-Takagi, Akiko, Yagishita, Sho, Nakamura, Mayumi, Shirai, Fukutoshi, Wu, Yi I., Loshbaugh, Amanda L., Kuhlman, Brian, Hahn, Klaus M. +1 more·2015·Nature
DOI 10.1038/nature15257PMC PMC4634641
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Abstract

Dendritic spines are the major loci of synaptic plasticity and are considered as possible structural correlates of memory. Nonetheless, systematic manipulation of specific subsets of spines in the cortex has been unattainable, and thus, the link between spines and memory has been correlational. We developed a novel synaptic optoprobe, AS-PaRac1 (activated synapse targeting photoactivatable Rac1), that can label recently potentiated spines specifically, and induce the selective shrinkage of AS-PaRac1-containing spines. In vivo imaging of AS-PaRac1 revealed that a motor learning task induced substantial synaptic remodelling in a small subset of neurons. The acquired motor learning was disrupted by the optical shrinkage of the potentiated spines, whereas it was not affected by the identical manipulation of spines evoked by a distinct motor task in the same cortical region. Taken together, our results demonstrate that a newly acquired motor skill depends on the formation of a task-specific dense synaptic ensemble.

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  • Synaptic plasticity and learning are associated with changes in spine morphology.

    “mounting evidence indicates that synaptic plasticity and learning are associated with changes in spine morphology”

    From The E3 ubiquitin ligase IDOL regulates synaptic ApoER2 levels and is important for plasticity and learning (2017) · cites this paper as Hayashi-Takagi et al., 2015

  • Spine morphological changes depend on NMDAR activation.

    “and that these morphological changes depend on NMDAR activation (Gambino et al., 2014; Hayashi-Takagi et al., 2015)”

    From The E3 ubiquitin ligase IDOL regulates synaptic ApoER2 levels and is important for plasticity and learning (2017) · cites this paper as Hayashi-Takagi et al., 2015

    The ‘Selective spine shrinkage by AS-PaRac1’ section attributes the observed shrinkage to photoactivated Rac1, but does not test whether it depends on NMDAR activation. A search for NMDAR activation and NMDA receptor blockade found receptor-related references, but no passage in our copy establishes the claimed dependence of spine morphological changes. (LLM, conf=0.73)

    Experimental — accuracy not yet measured

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Authors
Hayashi-Takagi, A., Yagishita, S., Nakamura, M., Shirai, F., Wu, Y. I., Loshbaugh, A. L., Kuhlman, B., Hahn, K. M., & Kasai, H.
Affiliations
  • Japan Science and Technology Agency (JP)
  • Center for Disease Biology and Integrative Medicine, The University of Tokyo (JP)
  • UConn Health (US)
  • University of North Carolina at Chapel Hill (US)
Corresponding author
Kasai, Haruo
ORCID
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
2015-09
Volume
525
Issue
7569
Pages
333-338
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
DOI
10.1038/nature15257
PMC
PMC4634641
OpenAlex
W1440335865
Cited by
624
References
7
Citations by year
2026: 34 · 2025: 42 · 2024: 47 · 2023: 59 · 2022: 71 · 2021: 61 · 2020: 59 · 2019: 66 · 2018: 62 · 2017: 78 · 2016: 41 · 2015: 4
FWCI
25.88
Type
journal article
Language
en
Open access
Licence
www.springer.com/tdm (tdm)
Locations
  • Nature (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • Carolina Digital Repository (University of North Carolina at Chapel Hill) (submittedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
  • UNC Libraries
Funders
  • Japan Agency for Medical Research and Development
  • Ministry of Education, Culture, Sports, Science and Technology (24116003, 23689055)
  • National Institutes of Health
  • Japan Society for the Promotion of Science (24116001, 24116003, 26293260, 26221001)
  • National Institute of General Medical Sciences (R01GM102924, GM102924)
  • National Institute of Neurological Disorders and Stroke (NS071216, R21NS071216)
  • Precursory Research for Embryonic Science and Technology
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Motor Control and Adaptation
Keywords
dendritic spines, synaptic plasticity, motor learning, motor cortex, optical erasure, synaptic remodeling, in vivo imaging
MeSH terms
Animals, Hippocampus, Light, Male, Memory, Motor Cortex, Motor Skills, Neuronal Plasticity, Synapses, Molecular Probes, Long-Term Potentiation, Rotarod Performance Test, Dendritic Spines, Mice, Spatio-Temporal Analysis, In Vitro Techniques

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