The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Hoxb5 marks long-term haematopoietic stem cells and reveals a homogenous perivascular niche
Open accessAbstract
Haematopoietic stem cells (HSCs) are arguably the most extensively characterized tissue stem cells. Since the identification of HSCs by prospective isolation, complex multi-parameter flow cytometric isolation of phenotypic subsets has facilitated studies on many aspects of HSC biology, including self-renewal, differentiation, ageing, niche, and diversity. Here we demonstrate by unbiased multi-step screening, identification of a single gene, homeobox B5 (Hoxb5, also known as Hox-2.1), with expression in the bone marrow that is limited to long-term (LT)-HSCs in mice. Using a mouse single-colour tri-mCherry reporter driven by endogenous Hoxb5 regulation, we show that only the Hoxb5(+) HSCs exhibit long-term reconstitution capacity after transplantation in primary transplant recipients and, notably, in secondary recipients. Only 7-35% of various previously defined immunophenotypic HSCs are LT-HSCs. Finally, by in situ imaging of mouse bone marrow, we show that >94% of LT-HSCs (Hoxb5(+)) are directly attached to VE-cadherin(+) cells, implicating the perivascular space as a near-homogenous location of LT-HSCs.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“By devising a simple approach to visualize inferred RNA content as a function of CytoTRACE (Fig. 3D, top), we observed a distinct valley in RNA abundance that coincided with elevated expression of Hoxb5, a marker of long-term or quiescent HSCs (35) (Fig. 3D, bottom).”
From Single-cell transcriptional diversity is a hallmark of developmental potential (2020) · cites this paper as [35]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339
Kiel, M. J., Yilmaz, Ö. H., Iwashita, T., Yilmaz, O. H., Terhorst, C., & Morrison, S. J. (2005) · Cell · doi:10.1016/j.cell.2005.05.026 · Cited by 3,168
Purification and Characterization of Mouse Hematopoietic Stem Cells
Spangrude, G. J., Heimfeld, S., & Weissman, I. L. (1988) · Science · doi:10.1126/science.2898810 · Cited by 2,908
Self-citationThe bone marrow niche for haematopoietic stem cells
Morrison, S. J., & Scadden, D. T. (2014) · Nature · doi:10.1038/nature12984 · Cited by 2,451
Long-Term Lymphohematopoietic Reconstitution by a Single CD34-Low/Negative Hematopoietic Stem Cell
Osawa, M., Hanada, K., Hamada, H., & Nakauchi, H. (1996) · Science · doi:10.1126/science.273.5272.242 · Cited by 2,141
Self-citationCD47 Is Upregulated on Circulating Hematopoietic Stem Cells and Leukemia Cells to Avoid Phagocytosis
Jaiswal, S., Jamieson, C. H. M., Pang, W. W., Park, C. Y., Chao, M. P., Majeti, R., Traver, D. J., van Rooijen, N., & Weissman, I. L. (2009) · Cell · doi:10.1016/j.cell.2009.05.046 · Cited by 1,578
Self-citationWhole-Brain Imaging with Single-Cell Resolution Using Chemical Cocktails and Computational Analysis
Susaki, E. A., Tainaka, K., Perrin, D., Kishino, F., Tawara, T., Watanabe, T. M., Yokoyama, C., Onoe, H., Eguchi, M., Yamaguchi, S., et al. (2014) · Cell · doi:10.1016/j.cell.2014.03.042 · Cited by 1,352
Regulation of oxidative stress by ATM is required for self-renewal of haematopoietic stem cells
Ito, K., Hirao, A., Arai, F., Matsuoka, S., Takubo, K., Hamaguchi, I., Nomiyama, K., Hosokawa, K., Sakurada, K., Nakagata, N., et al. (2004) · Nature · doi:10.1038/nature02989 · Cited by 1,175
Morrison, S. J., & Weissman, I. L. (1994) · Immunity · doi:10.1016/1074-7613(94)90037-x · Cited by 1,116
Self-citationPten dependence distinguishes haematopoietic stem cells from leukaemia-initiating cells
Yilmaz, Ö. H., Valdez, R., Theisen, B. K., Guo, W., Ferguson, D. O., Wu, H., & Morrison, S. J. (2006) · Nature · doi:10.1038/nature04703 · Cited by 1,113
Cited by
Sorted by citations
Single-cell transcriptional diversity is a hallmark of developmental potential
Gulati, G. S., Sikandar, S. S., Wesche, D. J., Manjunath, A., Bharadwaj, A., Berger, M. J., Ilagan, F., Kuo, A. H., Hsieh, R. W., Cai, S., et al. (2020) · Science · doi:10.1126/science.aax0249 · Cited by 1,571
Haematopoietic stem cell activity and interactions with the niche
Pinho, S., & Frenette, P. S. (2019) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-019-0103-9 · Cited by 1,064
A single-cell resolution map of mouse hematopoietic stem and progenitor cell differentiation
Nestorowa, S., Hamey, F. K., Pijuan Sala, B., Diamanti, E., Shepherd, M., Laurenti, E., Wilson, N. K., Kent, D. G., & Göttgens, B. (2016) · Blood · doi:10.1182/blood-2016-05-716480 · Cited by 768
Adult haematopoietic stem cell niches
Crane, G. M., Jeffery, E., & Morrison, S. J. (2017) · Nature Reviews Immunology · doi:10.1038/nri.2017.53 · Cited by 712
Tissue clearing and its applications in neuroscience
Ueda, H. R., Ertürk, A., Chung, K., Gradinaru, V., Chédotal, A., Tomančák, P., & Keller, P. (2020) · Nature reviews. Neuroscience · doi:10.1038/s41583-019-0250-1 · Cited by 650
Vitamin A-Retinoic Acid Signaling Regulates Hematopoietic Stem Cell Dormancy
Cabezas-Wallscheid, N., Buettner, F., Sommerkamp, P., Klimmeck, D., Ladel, L., Thalheimer, F. B., Pastor-Flores, D., Roma, L. P., Renders, S., Zeisberger, P., et al. (2017) · Cell · doi:10.1016/j.cell.2017.04.018 · Cited by 454
Clonal Hematopoiesis and Evolution to Hematopoietic Malignancies
Bowman, R. L., Busque, L., & Levine, R. L. (2018) · Cell stem cell · doi:10.1016/j.stem.2018.01.011 · Cited by 448
Differential cytokine contributions of perivascular haematopoietic stem cell niches
Asada, N., Kunisaki, Y., Pierce, H., Wang, Z., Fernandez, N., Birbrair, A., Ma’ayan, A., & Frenette, P. S. (2017) · Nature Cell Biology · doi:10.1038/ncb3475 · Cited by 453
Hematopoietic Stem Cells Are the Major Source of Multilineage Hematopoiesis in Adult Animals
Sawai, C. M., Babovic, S., Upadhaya, S., Knapp, D. J. H. F., Lavin, Y., Lau, C. M., Goloborodko, A., Feng, J., Fujisaki, J., Ding, L., et al. (2016) · Immunity · doi:10.1016/j.immuni.2016.08.007 · Cited by 381
“Several genetic markers were recently developed to identify HSC subsets with a particular lineage bias (Vwf, (Sanjuan-Pla et al., 2013)) or a superior reconstitution capacity (Hoxb5, (Chen et al., 2016)) during primary transplantations.”
Niches for Hematopoietic Stem Cells and Their Progeny
Wei, Q., & Frenette, P. S. (2018) · Immunity · doi:10.1016/j.immuni.2018.03.024 · Cited by 398
Clinical and Therapeutic Implications of Cancer Stem Cells
Clarke, M. F. (2019) · New England Journal of Medicine · doi:10.1056/nejmra1804280 · Cited by 355
Profiling cell identity and tissue architecture with single-cell and spatial transcriptomics
Gulati, G. S., D'Silva, J. P., Liu, Y., Wang, L., & Newman, A. M. (2024) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-024-00768-2 · Cited by 347
Ito, K., Turcotte, R., Cui, J., Zimmerman, S. E., Pinho, S., Mizoguchi, T., Arai, F., Runnels, J. M., Alt, C., Teruya‐Feldstein, J., et al. (2016) · Science · doi:10.1126/science.aaf5530 · Cited by 326
A network of trans-cortical capillaries as mainstay for blood circulation in long bones
Grüneboom, A., Hawwari, I., Weidner, D., Culemann, S., Müller, S., Henneberg, S., Brenzel, A., Merz, S. F., Bornemann, L., Zec, K., et al. (2018) · Nature Metabolism · doi:10.1038/s42255-018-0016-5 · Cited by 310
Resolving early mesoderm diversification through single-cell expression profiling
Scialdone, A., Tanaka, Y., Jawaid, W., Moignard, V., Wilson, N. K., Macaulay, I. C., Marioni, J. C., & Göttgens, B. (2016) · Nature · doi:10.1038/nature18633 · Cited by 293
Niches that regulate stem cells and hematopoiesis in adult bone marrow
Comazzetto, S., Shen, B., & Morrison, S. J. (2021) · Developmental Cell · doi:10.1016/j.devcel.2021.05.018 · Cited by 312
Live-animal imaging of native haematopoietic stem and progenitor cells
Christodoulou, C., Spencer, J. A., Yeh, S.-C. A., Turcotte, R., Kokkaliaris, K. D., Panero, R., Ramos, A., Guo, G., Seyedhassantehrani, N., Esipova, T. V., et al. (2020) · Nature · doi:10.1038/s41586-020-1971-z · Cited by 265
Targeting the perivascular niche sensitizes disseminated tumour cells to chemotherapy
Carlson, P., Dasgupta, A., Grzelak, C. A., Kim, J., Barrett, A. S., Coleman, I. M., Shor, R. E., Goddard, E. T., Dai, J., Schweitzer, E. M., et al. (2019) · Nature Cell Biology · doi:10.1038/s41556-018-0267-0 · Cited by 248
Single-cell analysis reveals the continuum of human lympho-myeloid progenitor cells
Karamitros, D., Stoilova, B., Aboukhalil, Z., Hamey, F., Reinisch, A., Samitsch, M., Quek, L. S., Otto, G., Repapi, E., Doondeea, J. B., et al. (2017) · Nature Immunology · doi:10.1038/s41590-017-0001-2 · Cited by 277
Regulation of adult stem cell quiescence and its functions in the maintenance of tissue integrity
de Morrée, A., & Rando, T. A. (2023) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-022-00568-6 · Cited by 258
Depleting myeloid-biased haematopoietic stem cells rejuvenates aged immunity
Ross, J., Myers, L. M., Noh, J. J., Collins, M. M., Carmody, A., Messer, R. J., Dhuey, E., Hasenkrug, K. J., & Weissman, I. L. (2024) · Nature · doi:10.1038/s41586-024-07238-x · Cited by 245
Self-citationLineage-Biased Hematopoietic Stem Cells Are Regulated by Distinct Niches
Pinho, S., Marchand, T., Yang, E. M., Wei, Q., Nerlov, C., & Frenette, P. S. (2018) · Developmental Cell · doi:10.1016/j.devcel.2018.01.016 · Cited by 235
A single-cell hematopoietic landscape resolves 8 lineage trajectories and defects in Kit mutant mice
Dahlin, J. S., Hamey, F. K., Pijuan-Sala, B., Shepherd, M., Lau, W. W. Y., Nestorowa, S., Weinreb, C., Wolock, S., Hannah, R., Diamanti, E., et al. (2018) · Blood · doi:10.1182/blood-2017-12-821413 · Cited by 233
Haematopoietic stem cell self-renewal in vivo and ex vivo
Wilkinson, A. C., Igarashi, K. J., & Nakauchi, H. (2020) · Nature Reviews Genetics · doi:10.1038/s41576-020-0241-0 · Cited by 226
Self-citationStem cell homing: From physiology to therapeutics
Liesveld, J. L., Sharma, N., & Aljitawi, O. S. (2020) · Stem Cells · doi:10.1002/stem.3242 · Cited by 222
Sinha, R., Stanley, G., Gulati, G. S., Ezran, C., Travaglini, K. J., Wei, E., Chan, C. K. F., Nabhan, A. N., Su, T., Morganti, R. M., et al. (2017) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/125724 · Cited by 206
Self-citationHeterogeneous Responses of Hematopoietic Stem Cells to Inflammatory Stimuli Are Altered with Age
Mann, M., Mehta, A., de Boer, C. G., Kowalczyk, M. S., Lee, K., Haldeman, P., Rogel, N., Knecht, A. R., Farouq, D., Regev, A., et al. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.11.056 · Cited by 188
Hematopoiesis and Cardiovascular Disease
Poller, W. C., Nahrendorf, M., & Świrski, F. K. (2020) · Circulation Research · doi:10.1161/circresaha.120.315895 · Cited by 191
Dormant tumour cells, their niches and the influence of immunity
Goddard, E. T., Božić, I., Riddell, S. R., & Ghajar, C. M. (2018) · Nature Cell Biology · doi:10.1038/s41556-018-0214-0 · Cited by 180
Bone Vasculature and Bone Marrow Vascular Niches in Health and Disease
Chen, J., Hendriks, M., Chatzis, A., Ramasamy, S. K., & Kusumbe, A. P. (2020) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.4171 · Cited by 180
Self-citationRegulation of Hematopoiesis and Osteogenesis by Blood Vessel–Derived Signals
Ramasamy, S. K., Kusumbe, A. P., Itkin, T., Gur‐Cohen, S., Lapidot, T., & Adams, R. H. (2016) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-111315-124936 · Cited by 176
Quek, L. S., Otto, G., Garnett, C., Lhermitte, L., Karamitros, D., Stoilova, B., Lau, I., Doondeea, J. B., Usukhbayar, B., Kennedy, A., et al. (2016) · The Journal of Experimental Medicine · doi:10.1084/jem.20151775 · Cited by 159
Complexity of bone marrow hematopoietic stem cell niche
Asada, N., Takeishi, S., & Frenette, P. S. (2017) · International Journal of Hematology · doi:10.1007/s12185-017-2262-9 · Cited by 162
Ca2+–mitochondria axis drives cell division in hematopoietic stem cells
Umemoto, T., Hashimoto, M., Matsumura, T., Nakamura‐Ishizu, A., & Suda, T. (2018) · The Journal of Experimental Medicine · doi:10.1084/jem.20180421 · Cited by 158
Organ-on-a-chip model of vascularized human bone marrow niches
Glaser, D. E., Curtis, M. B., Sariano, P. A., Rollins, Z. A., Shergill, B. S., Anand, A., Deely, A. M., Shirure, V. S., Anderson, L., Lowen, J. M., et al. (2022) · Biomaterials · doi:10.1016/j.biomaterials.2021.121245 · Cited by 146
Zhao, M., Tao, F., Venkatraman, A., Li, Z., Smith, S. E., Unruh, J., Chen, S., Ward, C., Qian, P., Perry, J. M., et al. (2019) · Cell Reports · doi:10.1016/j.celrep.2018.12.093 · Cited by 146
Himburg, H. A., Termini, C. M., Schlussel, L., Kan, J., Li, M., Zhao, L., Fang, T., Sasine, J. P., Chang, V. Y., & Chute, J. P. (2018) · Cell stem cell · doi:10.1016/j.stem.2018.07.003 · Cited by 149
Where Hematopoietic Stem Cells Live: The Bone Marrow Niche
Szade, K., Gulati, G. S., Chan, C. K. F., Kao, K. S., Miyanishi, M., Marjon, K. D., Sinha, R., George, B. M., Chen, J. Y., & Weissman, I. L. (2017) · Antioxidants and Redox Signaling · doi:10.1089/ars.2017.7419 · Cited by 145
Self-citationHaematopoietic stem cells in perisinusoidal niches are protected from ageing
Saçma, M., Pospiech, J., Bogeska, R., de Back, W., Mallm, J.-P., Sakk, V., Soller, K., Marka, G., Vollmer, A., Karns, R., et al. (2019) · Nature Cell Biology · doi:10.1038/s41556-019-0418-y · Cited by 133
Disruption of the Gut Microbiota Confers Cisplatin Resistance in Epithelial Ovarian Cancer
Chambers, L., Rhoades, E. E., Bharti, R., Braley, C., Tewari, S., Trestan, L., Alali, Z., Bayık, D., Lathia, J. D., Sangwan, N., et al. (2022) · Cancer Research · doi:10.1158/0008-5472.can-22-0455 · Cited by 129
Complex mammalian-like haematopoietic system found in a colonial chordate
Rosental, B., Kowarsky, M., Seita, J., Corey, D. M., Ishizuka, K. J., Palmeri, K. J., Chen, S.-Y., Sinha, R., Okamoto, J., Mantalas, G. L., et al. (2018) · Nature · doi:10.1038/s41586-018-0783-x · Cited by 128
Self-citationSäwen, P., Eldeeb, M., Erlandsson, E., Kristiansen, T. A., Laterza, C., Kokaia, Z., Karlsson, G., Yuan, J., Soneji, S., Mandal, P. K., et al. (2018) · eLife · doi:10.7554/elife.41258 · Cited by 120
Role of bone marrow macrophages in controlling homeostasis and repair in bone and bone marrow niches
Kaur, S., Raggatt, L. J., Batoon, L., Hume, D. A., Lévesque, J.-P., & Pettit, A. R. (2016) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2016.08.009 · Cited by 123
Poscablo, D. M., Worthington, A. K., Smith‐Berdan, S., Rommel, M. G. E., Manso, B. A., Adili, R., Mok, L., Reggiardo, R. E., Cool, T., Mogharrab, R., et al. (2024) · Cell · doi:10.1016/j.cell.2024.04.018 · Cited by 120
Structure and Functions of Blood Vessels and Vascular Niches in Bone
Ramasamy, S. K. (2017) · Stem Cells International · doi:10.1155/2017/5046953 · Cited by 125
The mechanisms of pathological extramedullary hematopoiesis in diseases
Yang, X., Chen, D., Long, H., & Zhu, B. (2020) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-020-03450-w · Cited by 114
Structural organization of the bone marrow and its role in hematopoiesis
Lucas, D. (2020) · Current Opinion in Hematology · doi:10.1097/moh.0000000000000621 · Cited by 123
Emerging agents that target signaling pathways to eradicate colorectal cancer stem cells
Silva, V. R., Santos, L. D. S., Dias, R. B., Quadros, C. A., & Bezerra, D. P. (2021) · 癌症：英文版 · doi:10.1002/cac2.12235 · Cited by 106
Myeloid Cell Origins, Differentiation, and Clinical Implications
Weiskopf, K., Schnorr, P. J., Pang, W. W., Chao, M. P., Chhabra, A., Seita, J., Feng, M., & Weissman, I. L. (2016) · Microbiology Spectrum · doi:10.1128/microbiolspec.mchd-0031-2016 · Cited by 104
Self-citationA Multi-Niche Microvascularized Human Bone-Marrow-on-a-Chip
Nelson, M. R., Ghoshal, D., Mejías, J. C., Rubio, D. A. F., Keith, E., & Roy, K. (2019) · Biomaterials · doi:10.1016/j.biomaterials.2021.120683 · Cited by 105
Tutorial: practical considerations for tissue clearing and imaging
Weiss, K. R., Voigt, F. F., Shepherd, D. P., & Huisken, J. (2021) · Nature Protocols · doi:10.1038/s41596-021-00502-8 · Cited by 108
Grigoryan, A., Guidi, N., Senger, K., Liehr, T., Soller, K., Marka, G., Vollmer, A., Markaki, Y., Leonhardt, H., Buske, C., et al. (2018) · Genome biology · doi:10.1186/s13059-018-1557-3 · Cited by 104
Zhou, J. R., Xu, J., Zhang, L., Liu, S., Ma, Y., Wen, X., Hao, J., Li, Z., Ni, Y., Li, X., et al. (2019) · Cell stem cell · doi:10.1016/j.stem.2018.11.023 · Cited by 98
Engineering Hematopoietic Stem Cells: Lessons from Development
Rowe, R. G., Mandelbaum, J., Zon, L. I., & Daley, G. Q. (2016) · Cell stem cell · doi:10.1016/j.stem.2016.05.016 · Cited by 101
A guidebook for DISCO tissue clearing
Molbay, M., Kolabas, Z. I., Todorov, M. I., Ohn, T., & Ertürk, A. (2021) · Molecular Systems Biology · doi:10.15252/msb.20209807 · Cited by 96
Wang, J., Kollárik, M., Ru, F., Sun, H., McNeil, B. D., Dong, X., Stephens, G. L., Korolevich, S., Brohawn, P. Z., Kolbeck, R., et al. (2017) · PLoS ONE · doi:10.1371/journal.pone.0185985 · Cited by 94
Bone marrow endothelial dysfunction promotes myeloid cell expansion in cardiovascular disease
Rohde, D., Vandoorne, K., Lee, I., Grune, J., Zhang, S., McAlpine, C. S., Schloss, M. J., Nayar, R., Courties, G., Frodermann, V., et al. (2021) · Nature Cardiovascular Research · doi:10.1038/s44161-021-00002-8 · Cited by 100
Hematopoietic stem cell fate through metabolic control
Ito, K., & Ito, K. (2018) · Experimental Hematology · doi:10.1016/j.exphem.2018.05.005 · Cited by 98
Adult stem cells and regenerative medicine—a symposium report
Cable, J., Fuchs, E. V., Weissman, I. L., Jasper, H., Glass, D. J., Rando, T. A., Blau, H. M., Debnath, S., Oliva, A. A., Park, S., et al. (2019) · Annals of the New York Academy of Sciences · doi:10.1111/nyas.14243 · Cited by 93
Self-citationKokkaliaris, K. D., Kunz, L., Cabezas‐Wallscheid, N., Christodoulou, C., Renders, S., Camargo, F. D., Trumpp, A., Scadden, D. T., & Schroeder, T. (2020) · Blood · doi:10.1182/blood.2020006574 · Cited by 93
Chapple, R. H., Tseng, Y.-J., Hu, T., Kitano, A., Takeichi, M., Hoegenauer, K. A., & Nakada, D. (2018) · Blood Advances · doi:10.1182/bloodadvances.2018016295 · Cited by 90
The many faces of hematopoietic stem cell heterogeneity
Crisan, M., & Dzierzak, E. A. (2016) · Development · doi:10.1242/dev.114231 · Cited by 97
The extracellular matrix of hematopoietic stem cell niches
Lee‐Thedieck, C., Schertl, P., & Klein, G. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2021.114069 · Cited by 96
Protection from UV light is an evolutionarily conserved feature of the haematopoietic niche
Kapp, F., Perlin, J. R., Hagedorn, E. J., Gansner, J. M., Schwarz, D. E., O’Connell, L. A., Johnson, N. S., Amemiya, C. T., Fisher, D. E., Wölfle, U., et al. (2018) · Nature · doi:10.1038/s41586-018-0213-0 · Cited by 91
Blood Vessels and Vascular Niches in Bone Development and Physiological Remodeling
Hendriks, M., & Ramasamy, S. K. (2020) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2020.602278 · Cited by 84
HLF expression defines the human hematopoietic stem cell state
Lehnertz, B., Chagraoui, J., MacRae, T., Tomellini, E., Corneau, S., Mayotte, N., Boivin, I., Durand, A., Gracias, D., & Sauvageau, G. (2021) · Blood · doi:10.1182/blood.2021010745 · Cited by 91
Metabolism as master of hematopoietic stem cell fate
Ito, K., Bonora, M., & Ito, K. (2018) · International Journal of Hematology · doi:10.1007/s12185-018-2534-z · Cited by 85
A time- and single-cell-resolved model of murine bone marrow hematopoiesis
Kuciński, I., Campos, J., Barile, M., Severi, F., Bohin, N., Moreira, P. N., Allen, L., Lawson, H., Haltalli, M. L. R., Kinston, S., et al. (2024) · Cell stem cell · doi:10.1016/j.stem.2023.12.001 · Cited by 87
Molecular and cellular mechanisms of aging in hematopoietic stem cells and their niches
Zhang, L., Mack, R. M., Breslin, P., & Zhang, J. (2020) · Journal of Hematology & Oncology · doi:10.1186/s13045-020-00994-z · Cited by 80
Muto, Y., Nishiyama, M., Nita, A., Moroishi, T., & Nakayama, K. I. (2017) · Nature Communications · doi:10.1038/ncomms16114 · Cited by 80
IL-6 blockade reverses bone marrow failure induced by human acute myeloid leukemia
Zhang, T. Y., Dutta, R., Benard, B. A., Zhao, F., Yin, R. R., & Majeti, R. (2020) · Science Translational Medicine · doi:10.1126/scitranslmed.aax5104 · Cited by 77
Intravital Imaging Reveals Motility of Adult Hematopoietic Stem Cells in the Bone Marrow Niche
Upadhaya, S., Krichevsky, O., Akhmetzyanova, I., Sawai, C. M., Fooksman, D., & Reizis, B. (2020) · Cell stem cell · doi:10.1016/j.stem.2020.06.003 · Cited by 74
Multiphoton intravital microscopy of rodents
Scheele, C. L. G. J., Herrmann, D., Yamashita, E., Celso, C. L., Jenne, C. N., Oktay, M. H., Entenberg, D. R., Friedl, P., Weigert, R., Meijboom, F. L. B., et al. (2022) · Nature Reviews Methods Primers · doi:10.1038/s43586-022-00168-w · Cited by 75
Next-generation imaging of the skeletal system and its blood supply
Grüneboom, A., Kling, L., Christiansen, S. H., Mill, L., Maier, A., Engelke, K., Quick, H. H., Schett, G. A., & Gunzer, M. (2019) · Nature Reviews Rheumatology · doi:10.1038/s41584-019-0274-y · Cited by 73
Cell cycle regulation of hematopoietic stem or progenitor cells
Hao, S., Chen, C., & Cheng, T. (2016) · International Journal of Hematology · doi:10.1007/s12185-016-1984-4 · Cited by 76
Role of angiocrine signals in bone development, homeostasis and disease
Sivan, U., Angelis, J. E. D., & Kusumbe, A. P. (2019) · Open Biology · doi:10.1098/rsob.190144 · Cited by 75
The evolving view of the hematopoietic stem cell niche
Beerman, I., Luis, T. C., Singbrant, S., Celso, C. L., & Méndez‐Ferrer, S. (2017) · Experimental Hematology · doi:10.1016/j.exphem.2017.01.008 · Cited by 74
Hematopoietic stem cell-independent hematopoiesis and the origins of innate-like B lymphocytes
Ghosn, E., Yoshimoto, M., Nakauchi, H., Weissman, I. L., & Herzenberg, L. A. (2019) · Development · doi:10.1242/dev.170571 · Cited by 73
Self-citationTissue Clearing and Its Application to Bone and Dental Tissues
Jing, D., Yi, Y., Luo, W., Zhang, S., Yuan, Q., Wang, J., Lachika, E., Zhao, Z., & Zhao, H. (2019) · Journal of Dental Research · doi:10.1177/0022034519844510 · Cited by 71
Interferon Gamma Mediates Hematopoietic Stem Cell Activation and Niche Relocalization through BST2
Florez, M. A., Matatall, K. A., Jeong, Y., Ortinau, L. C., Shafer, P. W., Lynch, A. M., Jaksik, R., Kimmel, M., Park, D., & King, K. Y. (2020) · Cell Reports · doi:10.1016/j.celrep.2020.108530 · Cited by 65
Ye, J., Lin, Y., Liao, Z., Gao, X., Lu, C. Y., Lu, L., Huang, J., Huang, X., Huang, S., Yu, H., et al. (2024) · npj Precision Oncology · doi:10.1038/s41698-024-00752-1 · Cited by 64
Self-citationTwo Niches in the Bone Marrow: A Hypothesis on Life-long T Cell Memory
Rosa, F. D. (2016) · Trends in Immunology · doi:10.1016/j.it.2016.05.004 · Cited by 61
The Bone Marrow Microenvironment for Hematopoietic Stem Cells
Lucas, D. (2017) · Advances in experimental medicine and biology · doi:10.1007/978-3-319-69194-7_2 · Cited by 63
Bone marrow dendritic cells regulate hematopoietic stem/progenitor cell trafficking
Zhang, J., Supakorndej, T., Krambs, J. R., Rao, M., Abou‐Ezzi, G., Ye, R. Y., Li, S., Trinkaus, K. M., & Link, D. C. (2019) · Journal of Clinical Investigation · doi:10.1172/jci124829 · Cited by 60
Current insights into the bone marrow niche: From biology in vivo to bioengineering ex vivo
Xiao, Y., McGuinness, C. S., Doherty-Boyd, W. S., Salmerón‐Sánchez, M., Donnelly, H., & Dalby, M. J. (2022) · Biomaterials · doi:10.1016/j.biomaterials.2022.121568 · Cited by 65
Kara, N., Xue, Y., Zhao, Z., Murphy, M. M., Comazzetto, S., Lesser, A., Du, L., & Morrison, S. J. (2023) · Developmental Cell · doi:10.1016/j.devcel.2023.02.003 · Cited by 61
Celik, H., Koh, W. K., Kramer, A. C., Ostrander, E. L., Mallaney, C., Fisher, D. A. C., Xiang, J., Wilson, W. C., Martens, A., Kothari, A., et al. (2018) · Cancer Cell · doi:10.1016/j.ccell.2018.10.008 · Cited by 59
Bernitz, J. M., Daniel, M. G., Fstkchyan, Y. S., & Moore, K. A. (2017) · Blood · doi:10.1182/blood-2016-11-752923 · Cited by 58
The Bone Marrow Microenvironment in Health and Myeloid MalignancyPreprint
Galán-Díez, M., Cuesta‐Domínguez, Á., & Kousteni, S. (2017) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a031328 · Cited by 59
Hematopoietic Stem Cell Niche During Homeostasis, Malignancy, and Bone Marrow Transplantation
Man, Y., Yao, X., Yang, T., & Wang, Y. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.621214 · Cited by 58
A Single Cell Transcriptomic Atlas Characterizes Aging Tissues in the MousePreprint
Pisco, A. O., McGeever, A., Schaum, N., Karkanias, J., Neff, N. F., Darmanis, S., Wyss‐Coray, T., & Quake, S. R. (2019) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/661728 · Cited by 57
The Source and Dynamics of Adult Hematopoiesis: Insights from Lineage Tracing
Pucella, J. N., Upadhaya, S., & Reizis, B. (2020) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-020520-114601 · Cited by 53
Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes
Zhang, M., Dong, Y., Hu, F., Yang, D., Zhao, Q., Lv, C., Wang, Y., Xia, C., Weng, Q., Liu, X., et al. (2018) · Nature Immunology · doi:10.1038/s41590-018-0046-x · Cited by 53
Self-citationBlood on the tracks: hematopoietic stem cell-endothelial cell interactions in homing and engraftment
Perlin, J. R., Sporrij, A., & Zon, L. I. (2017) · Journal of Molecular Medicine · doi:10.1007/s00109-017-1559-8 · Cited by 52
Unperturbed vs. post-transplantation hematopoiesis: both in vivo but different
Busch, K., & Rodewald, H.-R. (2016) · Current Opinion in Hematology · doi:10.1097/moh.0000000000000250 · Cited by 52
Human HOX Proteins Use Diverse and Context-Dependent Motifs to Interact with TALE Class Cofactors
Dard, A., Reboulet, J., Jia, Y. Y., Bleicher, F., Duffraisse, M., Vanaker, J.-M., Forcet, C., & Merabet, S. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.02.070 · Cited by 52
Qian, P., Kumar, B. D., He, X., Nolte, C., Gogol, M., Ahn, Y., Chen, S., Li, Z., Xu, H., Perry, J. M., et al. (2018) · Cell stem cell · doi:10.1016/j.stem.2018.04.012 · Cited by 51
Advances in spatial transcriptomics and its application in the musculoskeletal system
Wang, H., Cheng, P., Wang, J., Lv, H., Han, J., Hou, Z., Xu, R., & Chen, W. (2025) · Bone Research · doi:10.1038/s41413-025-00429-w · Cited by 53
Hostile takeover: how tumours hijack pre‐existing vascular environments to thrive
Winkler, F. (2017) · The Journal of Pathology · doi:10.1002/path.4904 · Cited by 50
Rabe, J. L., Hernandez, G., Chavez, J. S., Mills, T. S., Nerlov, C., & Pietras, E. M. (2020) · Experimental Hematology · doi:10.1016/j.exphem.2019.12.003 · Cited by 49
Metadata
- Authors
- Chen, J. Y., Miyanishi, M., Wang, S. K., Yamazaki, S., Sinha, R., Kao, K. S., Seita, J., Sahoo, D., Nakauchi, H., & Weissman, I. L.
- Affiliations
- Stanford Medicine (US)
- Institute of Medical Science, The University of Tokyo (JP)
- Institute for Stem Cell Biology and Regenerative Medicine (US)
- Corresponding author
- Miyanishi, Masanori
- Weissman, Irving L.
- ORCID
- Venue
- Nature
- Published
- 2016-02
- Volume
- 530
- Issue
- 7589
- Pages
- 223-227
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- PMC
- PMC4854608
- OpenAlex
- W2261428502
- Cited by
- 345
- References
- 34
- Citations by year
- 2026: 9 · 2025: 23 · 2024: 25 · 2023: 23 · 2022: 34 · 2021: 48 · 2020: 38 · 2019: 35 · 2018: 39 · 2017: 45 · 2016: 25
- FWCI
- 45.68
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- Japan Society for the Promotion of Science (25640044, 16H02661)
- National Heart, Lung, and Blood Institute (R01HL058770, F30HL122096, U01HL099999)
- National Cancer Institute (R00CA151673, R01CA086065)
- National Institute of General Medical Sciences (T32GM007365)
- Topics
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
- Health Sciences › Medicine › Hematology › Blood disorders and treatments
- Keywords
- HOXB5, hematopoietic stem cells, perivascular niche, endothelial cells, VE-cadherin, HSC niche, HSC self-renewal, bone marrow
- MeSH terms
- Cell Self Renewal, Cadherin 5, Animals, Bone Marrow, Gene Expression Regulation, Hematopoietic Stem Cells, Male, Mice, Inbred C57BL, Biomarkers, Antigens, CD, Cadherins, Immunophenotyping, Genes, Reporter, Hematopoietic Stem Cell Transplantation, Homeodomain Proteins, Mice, Stem Cell Niche
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
