Cited by 3,153CRISPR and Genetic EngineeringRNA regulation and diseaseRNA Interference and Gene Delivery

The DOI matches its Crossref record; 101 of the 102 references it cites were found in a registry or an index.

CRISPR-Cas systems for editing, regulating and targeting genomes

Open access

Sander, Jeffry D, Joung, J Keith·2014·Nature Biotechnology

Open this paper in CoChat — free account

Abstract

Targeted genome editing using engineered nucleases has rapidly gone from being a niche technology to a mainstream method used by many biological researchers. This widespread adoption has been largely fueled by the emergence of the clustered, regularly interspaced, short palindromic repeat (CRISPR) technology, an important new approach for generating RNA-guided nucleases, such as Cas9, with customizable specificities. Genome editing mediated by these nucleases has been used to rapidly, easily and efficiently modify endogenous genes in a wide variety of biomedically important cell types and in organisms that have traditionally been challenging to manipulate genetically. Furthermore, a modified version of the CRISPR-Cas9 system has been developed to recruit heterologous domains that can regulate endogenous gene expression or label specific genomic loci in living cells. Although the genome-wide specificities of CRISPR-Cas9 systems remain to be fully defined, the power of these systems to perform targeted, highly efficient alterations of genome sequence and gene expression will undoubtedly transform biological research and spur the development of novel molecular therapeutics for human disease.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1038/nbt.2842

Cites

101 of 101 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339
Genome engineering using the CRISPR-Cas9 system
Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott, D. A., & Zhang, F. (2013) · Nature Protocols · doi:10.1038/nprot.2013.143 · Cited by 11,133
RNA-Guided Human Genome Engineering via Cas9
Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210
CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes
Barrangou, R., Fremaux, C., Deveau, H., Richards, M., Boyaval, P., Moineau, S., Romero, D. A., & Horvath, P. (2007) · Science · doi:10.1126/science.1138140 · Cited by 5,947
Genome-scale CRISPR-Cas9 knockout screening in human cells
Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott, D. A., Mikkelsen, T. S., Heckl, D., Ebert, B. L., Root, D. E., Doench, J. G., et al. (2014) · Science · doi:10.1126/science.1247005 · Cited by 5,234
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
Qi, L. S., Larson, M. H., Gilbert, L. A., Doudna, J. A., Weissman, J. S., Arkin, A. P., & Lim, W. A. (2013) · Cell · doi:10.1016/j.cell.2013.02.022 · Cited by 5,016
DNA targeting specificity of RNA-guided Cas9 nucleases
Hsu, P. D., Scott, D. A., Weinstein, J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2647 · Cited by 4,741
CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
Gilbert, L. A., Larson, M. H., Morsut, L., Liu, Z., Brar, G. A., Torres, S. E., Stern-Ginossar, N., Brandman, O., Whitehead, E. H., Doudna, J. A., et al. (2013) · Cell · doi:10.1016/j.cell.2013.06.044 · Cited by 3,696
One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering
Wang, H., Yang, H., Shivalila, C. S., Dawlaty, M. M., Cheng, A. W., Zhang, F., & Jaenisch, R. (2013) · Cell · doi:10.1016/j.cell.2013.04.025 · Cited by 3,495

Flagged first, then by citations

Show all 102 references

1 of the 102 references couldn’t be matched to a known paper.

Sorted by citations

Items 601–700 of 1,015

Comparative assessments of CRISPR-Cas nucleases’ cleavage efficiency in planta
Johnson, R. A., Gurevich, V., Hayut, S. F., Samach, A., & Levy, A. A. (2014) · Plant Molecular Biology · doi:10.1007/s11103-014-0266-x · Cited by 76
Hypomorphic mutations inTRNT1cause retinitis pigmentosa with erythrocytic microcytosis
DeLuca, A. P., Whitmore, S. S., Barnes, J., Sharma, T. P., Westfall, T. A., Scott, C. A., Weed, M. C., Wiley, J. S., Wiley, L. A., Johnston, R. M., et al. (2015) · Human Molecular Genetics · doi:10.1093/hmg/ddv446 · Cited by 77
Neurotrophin Signaling and Stem Cells—Implications for Neurodegenerative Diseases and Stem Cell Therapy
Pramanik, S., Sulistio, Y. A., & Heese, K. (2016) · Molecular Neurobiology · doi:10.1007/s12035-016-0214-7 · Cited by 77
Expanding C–T base editing toolkit with diversified cytidine deaminases
Cheng, T., Li, S., Yuan, B., Wang, X., Zhou, W., & Qiu, Z. (2019) · Nature Communications · doi:10.1038/s41467-019-11562-6 · Cited by 74
Precision genome engineering in lactic acid bacteria
van Pijkeren, J. P., & Britton, R. A. (2014) · Microbial Cell Factories · doi:10.1186/1475-2859-13-s1-s10 · Cited by 78
Co-evolution of strain design methods based on flux balance and elementary mode analysis
Machado, D., & Herrgård, M. J. (2015) · Metabolic Engineering Communications · doi:10.1016/j.meteno.2015.04.001 · Cited by 76
Development and Application of CRISPR/Cas in Microbial Biotechnology
Ding, W., Zhang, Y., & Shi, S. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.00711 · Cited by 81
Redox-Responsive Phase-Separating Peptide as a Universal Delivery Vehicle for CRISPR/Cas9 Genome Editing Machinery
Sun, Y., Xu, X., Chen, L., Chew, W. L., Yuan, P., & Miserez, A. (2023) · ACS Nano · doi:10.1021/acsnano.3c02669 · Cited by 77
Synthetic Gene Circuits for Regulation of Next‐Generation Cell‐Based Therapeutics
Teixeira, A. P., & Fussenegger, M. (2023) · Advanced Science · doi:10.1002/advs.202309088 · Cited by 79
Cameo: A Python Library for Computer Aided Metabolic Engineering and Optimization of Cell Factories
Cardoso, J., Jensen, K. L., Lieven, C., Hansen, A. S. L., Galkina, S., Beber, M. E., Özdemir, E., Herrgård, M. J., Redestig, H., & Sonnenschein, N. (2018) · ACS Synthetic Biology · doi:10.1021/acssynbio.7b00423 · Cited by 75
Non‐Viral CRISPR/Cas Gene Editing In Vitro and In Vivo Enabled by Synthetic Nanoparticle Co‐Delivery of Cas9 mRNA and sgRNA
Miller, J. B., Zhang, S., Kós, P., Xiong, H., Zhou, K., Perelman, S. S., Zhu, H., & Siegwart, D. J. (2016) · Angewandte Chemie · doi:10.1002/ange.201610209 · Cited by 74
CRISPR-based genome editing in wheat: a comprehensive review and future prospects
Kumar, R., Kaur, A., Pandey, A., Mamrutha, H. M., & Singh, G. P. (2019) · Molecular Biology Reports · doi:10.1007/s11033-019-04761-3 · Cited by 70
CRISPR/Cas9 in lepidopteran insects: Progress, application and prospects
Li, J., Shi, Y., Wu, J., Li, H., Smagghe, G. J., & Liu, T. (2021) · Journal of Insect Physiology · doi:10.1016/j.jinsphys.2021.104325 · Cited by 76
Design and synthesis of DNA hydrogel based on EXPAR and CRISPR/Cas14a for ultrasensitive detection of creatine kinase MB
Chen, M., Zhang, J., Peng, Y. Y., Bai, J., Li, S., Han, D., Ren, S., Qin, K., Zhou, H., Han, T., et al. (2022) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2022.114792 · Cited by 74
How genetic errors in GPCRs affect their function: Possible therapeutic strategies
Stoy, H., & Gurevich, V. V. (2015) · Genes & Diseases · doi:10.1016/j.gendis.2015.02.005 · Cited by 77
Spermatogonial stem cell autotransplantation and germline genomic editing: a future cure for spermatogenic failure and prevention of transmission of genomic diseases
Mulder, C. L., Zheng, Y., Jan, S. Z., Struijk, R. B., Repping, S., Hamer, G., & van Pelt, A. M. M. (2016) · Human Reproduction Update · doi:10.1093/humupd/dmw017 · Cited by 76
Mechanism of duplex DNA destabilization by RNA-guided Cas9 nuclease during target interrogation
Mekler, V. M., Станиславович, М. Л., & Severinov, K. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1619926114 · Cited by 74
Molecular breeding technologies and strategies for rust resistance in wheat ( Triticum aestivum ) for sustained food security
Savadi, S., Prasad, P., Kashyap, P. L., & Bhardwaj, S. C. (2017) · Plant Pathology · doi:10.1111/ppa.12802 · Cited by 74
CRISPR-Cas9 genome engineering: Treating inherited retinal degeneration
Burnight, E. R., Giacalone, J. C., Cooke, J. A., Thompson, J. R., Bohrer, L. R., Chirco, K. R., Drack, A. V., Fingert, J. H., Worthington, K. S., Wiley, L. A., et al. (2018) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2018.03.003 · Cited by 79
Reduction-dependent siderophore assimilation in a model pennate diatom
Coale, T. H., Moosburner, M., Horák, A., Oborník, M., Barbeau, K. A., & Allen, A. E. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1907234116 · Cited by 75
Gene Therapy Intervention in Neovascular Eye Disease: A Recent Update
Lin, F.-L., Wang, P.-Y., Chuang, Y.-F., Wang, J.-H., Wong, V. H. Y., Bui, B. V., & Liu, G.-S. (2020) · Molecular Therapy · doi:10.1016/j.ymthe.2020.06.029 · Cited by 74
Microbead models in glaucoma
Morgan, J. E., & Tribble, J. R. (2015) · Experimental Eye Research · doi:10.1016/j.exer.2015.06.020 · Cited by 74
CRISPR-Cas9 vectors for genome editing and host engineering in the baculovirus–insect cell system
Mabashi‐Asazuma, H., & Jarvis, D. L. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1705836114 · Cited by 73
CRISPR/Cas9-The ultimate weapon to battle infectious diseases?
Doerflinger, M., Forsyth, W. O., Ebert, G., Pellegrini, M., & Herold, M. J. (2016) · Cellular Microbiology · doi:10.1111/cmi.12693 · Cited by 73
CRISPR-Cas9 technology: applications in genome engineering, development of sequence-specific antimicrobials, and future prospects
de la Fuente‐Núñez, C., & Lu, T. K. (2016) · Integrative Biology · doi:10.1039/c6ib00140h · Cited by 75
Cracking the egg: An insight into egg hypersensitivity
Dhanapala, P., Silva, C. D., Doran, T. J., & Suphioglu, C. (2015) · Molecular Immunology · doi:10.1016/j.molimm.2015.04.016 · Cited by 78
ANNOgesic: a Swiss army knife for the RNA-seq based annotation of bacterial/archaeal genomes
Yu, S., Vogel, J., & Förstner, K. U. (2018) · GigaScience · doi:10.1093/gigascience/giy096 · Cited by 73
Cell-derived extracellular vesicles for CRISPR/Cas9 delivery: engineering strategies for cargo packaging and loading
Liang, Y., Iqbal, Z., Wang, J., Xu, L., Xu, X., Ouyang, K., Zhang, H., Lu, J., Duan, L., & Xia, J. (2022) · Biomaterials Science · doi:10.1039/d2bm00480a · Cited by 72
Synthetic epigenetics—towards intelligent control of epigenetic states and cell identity
Jurkowski, T. P., Ravichandran, M., & Stepper, P. (2015) · Clinical Epigenetics · doi:10.1186/s13148-015-0044-x · Cited by 73
Efficient Generation of Gene-Modified Pigs Harboring Precise Orthologous Human Mutation via CRISPR/Cas9-Induced Homology-Directed Repair in Zygotes
Zhou, X., Wang, L., Du, Y., Xie, F., Li, L., Liu, Y., Liu, C., Wang, S., Zhang, S., Huang, X., et al. (2015) · Human Mutation · doi:10.1002/humu.22913 · Cited by 72
Exploiting CRISPR–Cas immune systems for genome editing in bacteria
Barrangou, R., & van Pijkeren, J. (2015) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2015.10.003 · Cited by 75
Detection of target DNA with a novel Cas9/sgRNAs-associated reverse PCR (CARP) technique
Zhang, B., Wang, Q., Xu, X., Xia, Q., Long, F., Li, W., Shui, Y., Xia, X., & Wang, J. (2018) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-018-0873-5 · Cited by 72
Programmed knockout mutation of liver fluke granulin attenuates virulence of infection-induced hepatobiliary morbidity
Arunsan, P., Ittiprasert, W., Smout, M. J., Cochran, C. J., Mann, V. H., Chaiyadet, S., Karinshak, S. E., Sripa, B., Young, N. D., Sotillo, J., et al. (2019) · eLife · doi:10.7554/elife.41463 · Cited by 77
Long Non-coding RNA DANCR as an Emerging Therapeutic Target in Human Cancers
Jin, S.-J., Jin, M., Xia, B.-R., & Jin, W. (2019) · Frontiers in Oncology · doi:10.3389/fonc.2019.01225 · Cited by 70
Artificial intelligence and synthetic biology approaches for human gut microbiome
Kumar, P., Sinha, R., & Shukla, P. (2020) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2020.1850415 · Cited by 78
RNA‐Cleaving DNAzyme‐Based Amplification Strategies for Biosensing and Therapy
Su, J., Sun, C., Du, J., Xing, X., Wang, F., & Dong, H. (2023) · Advanced Healthcare Materials · doi:10.1002/adhm.202300367 · Cited by 72
Gene editing therapeutics based on mRNA delivery
Popovitz, J., Sharma, R. K., Hoshyar, R., Kim, B. S., Murthy, N., & Lee, K. (2023) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2023.115026 · Cited by 73
Recombinant messenger RNA technology and its application in cancer immunotherapy, transcript replacement therapies, pluripotent stem cell induction, and beyond
Vallazza, B., Petri, S., Poleganov, M. A., Eberle, F., Kuhn, A. N., & Şahin, U. (2015) · Wiley Interdisciplinary Reviews - RNA · doi:10.1002/wrna.1288 · Cited by 73
A chemical–genetic interaction map of small molecules using high‐throughput imaging in cancer cells
Breinig, M., Klein, F. A., Huber, W. K., & Boutros, M. (2015) · Molecular Systems Biology · doi:10.15252/msb.20156400 · Cited by 70
A versatile modular vector system for rapid combinatorial mammalian genetics
Albers, J., Danzer, C., Rechsteiner, M., Lehmann, H., Brandt, L. P., Hejhal, T., Catalano, A., Busenhart, P., Gonçalves, A. F., Brandt, S., et al. (2015) · Journal of Clinical Investigation · doi:10.1172/jci79743 · Cited by 73
A pathway from chromosome transfer to engineering resulting in human and mouse artificial chromosomes for a variety of applications to bio-medical challenges
Oshimura, M., Uno, N., Kazuki, Y., Katoh, M., & Inoue, T. (2015) · Chromosome Research · doi:10.1007/s10577-014-9459-z · Cited by 72
CRISPR/Cas9-Based Genome Editing in Mice by Single Plasmid Injection
Fujihara, Y., & Ikawa, M. (2014) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/b978-0-12-801185-0.00015-5 · Cited by 70
Efficient Screening of CRISPR/Cas9-Induced Events in Drosophila Using a Co-CRISPR Strategy
Kane, N. S., Vora, M., Varre, K. J., & Padgett, R. W. (2017) · G3 Genes Genomes Genetics · doi:10.1534/g3.116.036723 · Cited by 71
A simple and rapid approach to develop recombinant avian herpesvirus vectored vaccines using CRISPR/Cas9 system
Tang, N., Zhang, Y., Pedrera, M., Chang, P., Baigent, S. J., Moffat, K., Shen, Z., Nair, V., & Yao, Y. (2017) · Vaccine · doi:10.1016/j.vaccine.2017.12.025 · Cited by 71
Guidance of super-enhancers in regulation of IL-9 induction and airway inflammation
Xiao, X., Fan, Y., Li, J., Zhang, X., Lou, X., Dou, Y., Shi, X., Lan, P., Xiao, Y., Minze, L. J., et al. (2018) · The Journal of Experimental Medicine · doi:10.1084/jem.20170928 · Cited by 75
Current strategies for Site-Directed RNA Editing using ADARs
Montiel-González, M. F., Quiroz, J. F. D., & Rosenthal, J. J. C. (2018) · Methods · doi:10.1016/j.ymeth.2018.11.016 · Cited by 73
Targeting Non-Coding RNAs in Plants with the CRISPR-Cas Technology is a Challenge yet Worth Accepting
Basak, J., & Nithin, C. (2015) · Frontiers in Plant Science · doi:10.3389/fpls.2015.01001 · Cited by 72
Exploring the natural variation for reproductive thermotolerance in wild tomato species
Driedonks, N., Wolters‐Arts, M., Huber, H., de Boer, G.-J., Vriezen, W. H., Mariani, C., & Rieu, I. (2018) · Euphytica · doi:10.1007/s10681-018-2150-2 · Cited by 70
Quantitative affinity purification mass spectrometry: a versatile technology to study protein–protein interactions
Meyer, K., & Selbach, M. (2015) · Frontiers in Genetics · doi:10.3389/fgene.2015.00237 · Cited by 75
Synthetic biology advances for pharmaceutical production
Breitling, R., & Takano, E. (2015) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2015.02.004 · Cited by 72
The old and new face of craniofacial research: How animal models inform human craniofacial genetic and clinical data
Otterloo, E. V., Williams, T., & Artinger, K. (2016) · Developmental Biology · doi:10.1016/j.ydbio.2016.01.017 · Cited by 67
Genetic dissection of neural circuits underlying sexually dimorphic social behaviours
Bayless, D. W., & Shah, N. M. (2016) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2015.0109 · Cited by 73
Determining the Specificities of TALENs, Cas9, and Other Genome-Editing Enzymes
Pattanayak, V., Guilinger, J. P., & Liu, D. R. (2014) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/b978-0-12-801185-0.00003-9 · Cited by 70
CRISPR-typing PCR (ctPCR), a new Cas9-based DNA detection method
Wang, Q., Zhang, B., Xu, X., Long, F., & Wang, J. (2018) · Scientific Reports · doi:10.1038/s41598-018-32329-x · Cited by 70
USH2A Gene Editing Using the CRISPR System
Fuster‐García, C., García‐García, G., González-Romero, E., Jaijo, T., Sequedo, M. D., Ayuso, C., Vázquez‐Manrique, R. P., Millán, J. M., & Aller, E. (2017) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2017.08.003 · Cited by 70
CRISPR-Cas System: History and Prospects as a Genome Editing Tool in Microorganisms
Javed, M. R., Sadaf, M., Ahmed, T., Jamil, A., Nawaz, M., Abbas, H., & Ijaz, A. (2018) · Current Microbiology · doi:10.1007/s00284-018-1547-4 · Cited by 78
Towards understanding the biosynthetic pathway for ustilaginoidin mycotoxins in Ustilaginoidea virens
Li, Y., Wang, M., Liu, Z., Zhang, K., Cui, F., & Sun, W. (2019) · Environmental Microbiology · doi:10.1111/1462-2920.14572 · Cited by 69
Targeted deletion of BCL11A gene by CRISPR-Cas9 system for fetal hemoglobin reactivation: A promising approach for gene therapy of beta thalassemia disease
Khosravi, M. A., Abbasalipour, M., Concordet, J., Berg, J. V., Zeinali, S., Arashkia, A., Azadmanesh, K., Buch, T., & Karimipoor, M. (2019) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2019.04.042 · Cited by 71
An overview of designing and selection of sgRNAs for precise genome editing by the CRISPR-Cas9 system in plants
Uniyal, A. P., Mansotra, K., Yadav, S. K., & Kumar, V. S. (2019) · 3 Biotech · doi:10.1007/s13205-019-1760-2 · Cited by 70
CRISPR/Cas9 gene-editing strategies in cardiovascular cells
Vermersch, E., Jouve, C., & Hulot, J. (2019) · Cardiovascular Research · doi:10.1093/cvr/cvz250 · Cited by 74
Emerging approaches for restoration of hearing and vision
Kleinlogel, S., Vogl, C., Jeschke, M., Neef, J., & Moser, T. (2020) · Physiological Reviews · doi:10.1152/physrev.00035.2019 · Cited by 74
Unravelling the Role of Rhizospheric Plant-Microbe Synergy in Phytoremediation: A Genomic Perspective
Agarwal, P., Giri, B. S., & Rani, R. (2020) · Current Genomics · doi:10.2174/1389202921999200623133240 · Cited by 71
Antiviral therapies: advances and perspectives
Gonçalves, B. C., Barboza, M. G. L., Olak, A. P. S., Terezo, N. B., Nishi, L., Watanabe, M. A. E., Marinello, P. C., Rechenchoski, D. Z., Rocha, S. P. D., & Galhardi, L. C. F. (2020) · Fundamental and Clinical Pharmacology · doi:10.1111/fcp.12609 · Cited by 70
Innovative Therapeutic Approaches for Duchenne Muscular Dystrophy
Fortunato, F., Rossi, R., Falzarano, M. S., & Ferlini, A. (2021) · Journal of Clinical Medicine · doi:10.3390/jcm10040820 · Cited by 64
Novel lentiviral vectors with mutated reverse transcriptase for mRNA delivery of TALE nucleases
Mock, U., Riecken, K., Berdien, B., Qasim, W., Chan, E., Cathomen, T., & Fehse, B. (2014) · Scientific Reports · doi:10.1038/srep06409 · Cited by 68
Polycistronic tRNA and CRISPR guide-RNA enables highly efficient multiplexed genome engineering in human cells
Dong, F., Xie, K., Chen, Y., Yang, Y., & Mao, Y. (2016) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2016.11.129 · Cited by 75
Marker Recycling in Candida albicans through CRISPR-Cas9-Induced Marker Excision
Huang, M. Y., & Mitchell, A. P. (2017) · mSphere · doi:10.1128/msphere.00050-17 · Cited by 69
Midbrain-Hindbrain Boundary Morphogenesis: At the Intersection of Wnt and Fgf Signaling
Gibbs, H. C., Chang-Gonzalez, A. C., Hwang, W., Yeh, A. T., & Lekven, A. C. (2017) · Frontiers in Neuroanatomy · doi:10.3389/fnana.2017.00064 · Cited by 72
Nanoparticles-mediated CRISPR/Cas9 delivery: Recent advances in cancer treatment
Aghamiri, S., Talaei, S., Ghavidel, A. A., Zandsalimi, F., Masoumi, S., Hafshejani, N. H., & Jajarmi, V. (2020) · Journal of Drug Delivery Science and Technology · doi:10.1016/j.jddst.2020.101533 · Cited by 70
Genome typing of nonhuman primate models: implications for biomedical research
Haus, T., Ferguson, B. M., Rogers, J., Doxiadis, G. G. M., Certa, U., Rose, N. J., Teepe, R., Weinbauer, G. F., & Roos, C. (2014) · Trends in Genetics · doi:10.1016/j.tig.2014.05.004 · Cited by 68
CRISPR/Cas9-mediated conversion of eGFP- into Gal4-transgenic lines in zebrafish
Auer, T. O., Duroure, K., Concordet, J., & Bene, F. D. (2014) · Nature Protocols · doi:10.1038/nprot.2014.187 · Cited by 68
Programmable Live‐Cell CRISPR Imaging with Toehold‐Switch‐Mediated Strand Displacement
Hao, Y., Jiang, L., Li, Q., Zhang, L., Shi, J., Zhang, X., Aldalbahi, A. K., Wang, L., Fan, C., & Wang, F. (2020) · Angewandte Chemie International Edition · doi:10.1002/anie.202009062 · Cited by 68
Therapeutic nucleus-access BNCT drug combined CD47-targeting gene editing in glioblastoma
Chen, J., Dai, Q., Yang, Q., Bao, X., Zhou, Y., Zhong, H., Wu, L., Wang, T., Zhang, Z., Lu, Y., et al. (2022) · Journal of Nanobiotechnology · doi:10.1186/s12951-022-01304-0 · Cited by 68
Novel Plant Breeding Techniques Shake Hands with Cereals to Increase Production
Haroon, M., Zafar, M. M., Farooq, M. A., Afzal, R., Batool, M., Idrees, F., Babar, U., Khan, A. S., 默辉娟, Li, L., et al. (2022) · Plants · doi:10.3390/plants11081052 · Cited by 70
Fecal Microbiota Transplantation and Other Gut Microbiota Manipulation Strategies
Quaranta, G., Guarnaccia, A., Fancello, G., Agrillo, C., Iannarelli, F., Sanguinetti, M., & Masucci, L. (2022) · Microorganisms · doi:10.3390/microorganisms10122424 · Cited by 72
Efficient genomic correction methods in human iPS cells using CRISPR–Cas9 system
Li, H. L., Gee, P., Ishida, K., & Hotta, A. (2016) · Methods · doi:10.1016/j.ymeth.2015.10.015 · Cited by 62
The immunopathogenesis of birdshot chorioretinopathy; a bird of many feathers
Kuiper, J. J. W., Rothová, A., de Boer, J. H., & Radstake, T. R. D. J. (2014) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2014.11.003 · Cited by 67
Wnt-signalling pathways and microRNAs network in carcinogenesis: experimental and bioinformatics approaches
Onyido, E. K., Sweeney, E., & Nateri, A. S. (2016) · Molecular Cancer · doi:10.1186/s12943-016-0541-3 · Cited by 69
Emerging Role of CRISPR/Cas9 Technology for MicroRNAs Editing in Cancer Research
Aquino‐Jarquín, G. (2017) · Cancer Research · doi:10.1158/0008-5472.can-17-2142 · Cited by 70
Gene therapy strategies in the treatment of hypertrophic cardiomyopathy
Prondzynski, M., Mearini, G., & Carrier, L. (2018) · Pflügers Archiv - European Journal of Physiology · doi:10.1007/s00424-018-2173-5 · Cited by 68
CRISPR-induced double-strand breaks trigger recombination between homologous chromosome arms
Brunner, E., Yagi, R., Debrunner, M., Beck-Schneider, D., Burger, A., Escher, E., Mosimann, C., Hausmann, G., & Basler, K. (2019) · Life Science Alliance · doi:10.26508/lsa.201800267 · Cited by 65
Drug Inducible CRISPR/Cas Systems
Zhang, J., Chen, L., Zhang, J., & Wang, Y. (2019) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2019.07.015 · Cited by 77
Ex Vivo COL7A1 Correction for Recessive Dystrophic Epidermolysis Bullosa Using CRISPR/Cas9 and Homology-Directed Repair
Izmiryan, A., Ganier, C., Bovolenta, M., Schmitt, A., Mavilio, F., & Hovnanian, A. (2018) · Molecular Therapy Nucleic Acids · doi:10.1016/j.omtn.2018.06.008 · Cited by 68
Selection is required for efficient Cas9-mediated genome editing in Fusarium graminearum
Gardiner, D. M., & Kazan, K. (2017) · Fungal Biology · doi:10.1016/j.funbio.2017.11.006 · Cited by 67
Considerations in adapting CRISPR/Cas9 in nongenetic model plant systems
Shan, S., Soltis, P. S., Soltis, D. E., & Yang, B. (2020) · Applications in Plant Sciences · doi:10.1002/aps3.11314 · Cited by 71
Yeast metabolic engineering for the production of pharmaceutically important secondary metabolites
Rahmat, E., & Kang, Y. (2020) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-020-10587-y · Cited by 67
Inducible Degradation of Target Proteins through a Tractable Affinity-Directed Protein Missile System
Simpson, L. M., Macartney, T., Nardin, A., Fulcher, L. J., Röth, S., Testa, A., Maniaci, C., Ciulli, A., Ganley, I. G., & Sapkota, G. P. (2020) · Cell chemical biology · doi:10.1016/j.chembiol.2020.06.013 · Cited by 71
Optical Control of a CRISPR/Cas9 System for Gene Editing by Using Photolabile crRNA
Zhang, Y., Ling, X., Su, X., Zhang, S., 王, J. 璟. W., Zhang, P., Feng, W., Zhu, Y. Y., Liu, T., & Tang, X. (2020) · Angewandte Chemie International Edition · doi:10.1002/anie.202009890 · Cited by 67
Improving lignocellulosic biofuel production by CRISPR/Cas9‐mediated lignin modification in barley
Lee, J. H., Won, H. J., Tran, P. H. N., Lee, S., Kim, H., & Jung, J. H. (2021) · GCB Bioenergy · doi:10.1111/gcbb.12808 · Cited by 67
Homozygous might be hemizygous: CRISPR/Cas9 editing in iPSCs results in detrimental on-target defects that escape standard quality controls
Simkin, D., Papakis, V., Bustos, B. I., Ambrosi, C. M., Ryan, S. J., Baru, V., Williams, L. A., Dempsey, G. T., McManus, O. B., Landers, J. E., et al. (2022) · Stem Cell Reports · doi:10.1016/j.stemcr.2022.02.008 · Cited by 68
Targeted introduction of heritable point mutations into the plant mitochondrial genome
Forner, J., Kleinschmidt, D., Meyer, E. H., Fischer, A., Morbitzer, R., Lahaye, T., Schöttler, M. A., & Bock, R. (2022) · Nature Plants · doi:10.1038/s41477-022-01108-y · Cited by 66
Current updates of CRISPR/Cas9‐mediated genome editing and targeting within tumor cells: an innovative strategy of cancer management
Allemailem, K. S., Alsahli, M. A., Almatroudi, A., Alrumaihi, F., Alkhaleefah, F. K., Rahmani, A. H., & Khan, A. A. (2022) · 癌症：英文版 · doi:10.1002/cac2.12366 · Cited by 69
Biomaterial-assisted targeted and controlled delivery of CRISPR/Cas9 for precise gene editing
Iqbal, Z., Rehman, K., Xia, J., Shabbir, M., Zaman, M., Liang, Y., & Duan, L. (2023) · Biomaterials Science · doi:10.1039/d2bm01636b · Cited by 71
Random Splicing of Several Exons Caused by a Single Base Change in the Target Exon of CRISPR/Cas9 Mediated Gene Knockout
Kapahnke, M., Banning, A., & Tikkanen, R. (2016) · Cells · doi:10.3390/cells5040045 · Cited by 67
Chemically Controlled Epigenome Editing through an Inducible dCas9 System
Chen, T., Gao, D., Zhang, R., Zeng, G., Yan, H., Lim, E., & Liang, F. (2017) · Journal of the American Chemical Society · doi:10.1021/jacs.7b06555 · Cited by 65
Genome Editing in Mice Using CRISPR/Cas9 Technology
Hall, B., Cho, A., Limaye, A., Cho, K., Khillan, J. S., & Kulkarni, A. B. (2018) · Current Protocols in Cell Biology · doi:10.1002/cpcb.57 · Cited by 72
Zinc finger nuclease‐mediated targeting of multiple transgenes to an endogenous soybean genomic locus via non‐homologous end joining
Bonawitz, N. D., Ainley, W. M., Itaya, A., Chennareddy, S. R., Cicak, T., Effinger, K., Jiang, K., Mall, T. K., Marri, P. R., Samuel, J. P., et al. (2018) · Plant Biotechnology Journal · doi:10.1111/pbi.13012 · Cited by 66
Oxygen-sensitive methylation of ULK1 is required for hypoxia-induced autophagy
Li, J., Zhang, T., Ren, T., Liao, X., Hao, Y., Lim, J. S., Lee, J.-H., Li, M., Shao, J., & Liu, R. R. (2022) · Nature Communications · doi:10.1038/s41467-022-28831-6 · Cited by 68
The Convergence of Systems and Reductionist Approaches in Complex Trait Analysis
Williams, E. G., & Auwerx, J. H. (2015) · Cell · doi:10.1016/j.cell.2015.06.024 · Cited by 69
Complete Knockout of Endogenous Mdr1 (Abcb1) in MDCK Cells by CRISPR-Cas9
Simoff, I., Karlgren, M., Backlund, M., Lindström, A.-C., Gaugaz, F. Z., Matsson, P., & Artursson, P. (2016) · Journal of Pharmaceutical Sciences · doi:10.1016/s0022-3549(15)00171-9 · Cited by 69

Authors: Sander, J. D., & Joung, J. K.
Affiliations: Harvard University (US)
Corresponding author: Joung, J Keith
ORCID: Sander, Jeffry D
Joung, J Keith
Venue: Nature Biotechnology
Publisher: Springer Science and Business Media LLC
Published: 2014-03-02
Volume: 32
Issue: 4
Pages: 347-355
ISSN: 0733-222X (ISSN-L)
1087-0156
1546-1696
DOI: 10.1038/nbt.2842
PMC: PMC4022601
OpenAlex: W2039705224
Cited by: 3,153
References: 102
Citations by year: 2026: 79 · 2025: 153 · 2024: 167 · 2023: 166 · 2022: 233 · 2021: 225 · 2020: 271 · 2019: 298 · 2018: 379 · 2017: 368 · 2016: 384 · 2015: 300 · 2014: 124
FWCI: 36.95
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.springer.com/tdm (tdm)
Locations: Nature Biotechnology (publishedVersion)
PubMed (publishedVersion)
PubMed Central (acceptedVersion)
Funders: National Institute of General Medical Sciences (DP1GM105378, R01GM088040)
National Center for Chronic Disease Prevention and Health Promotion (DP1 GM105378)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Keywords: CRISPR/Cas9, genome editing, RNA-guided nucleases, engineered nucleases, gene expression regulation, targeted mutagenesis, molecular therapeutics
MeSH terms: Animals, Genetic Engineering, Humans, Rabbits, Genomics, Mice, Rats, Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR-Cas Systems

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

CRISPR-Cas systems for editing, regulating and targeting genomes

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks