Enzymatic assembly of DNA molecules up to several hundred kilobases

Foundations for engineering biology

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SH2-mediated steric occlusion of the C2 domain regulates autoinhibition of SHIP1 inositol 5-phosphatase

Drew, E. E., Nyvall, H. G., Parson, M. A. H., Talus, R. K., Burke, J. E., & Hansen, S. D. (2025) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2025.110788 · Cited by 6

Fusion crystallization reveals the behavior of both the 1TEL crystallization chaperone and the TNK1 UBA domain

Nawarathnage, S., Tseng, Y. J., Soleimani, S., Smith, T., Pedroza Romo, M. J., Abiodun, W. O., Egbert, C. M., Madhusanka, D., Bunn, D., Woods, B., et al. (2023) · Structure · doi:10.1016/j.str.2023.09.001 · Cited by 6

SynDLP is a dynamin-like protein of Synechocystis sp. PCC 6803 with eukaryotic features

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A Novel Biosensor for Ferrous Iron Developed via CoBiSe: A Computational Method for Rapid Biosensor Design

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Conformational variability of cyanobacterial ChlI, the AAA+ motor of magnesium chelatase involved in chlorophyll biosynthesis

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Characterization and Diversification of AraC/XylS Family Regulators Guided by Transposon Sequencing

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Exploring the Potential of the Model Cyanobacteria Synecho-Coccus PCC 7002 and PCC 7942 for the Photoproduction of High-Value Terpenes: Comparison With Synechocystis PCC 6803

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A De Novo Designed Protein with Versatile Metal Binding and Tunable Hydrolytic Activity

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Interplay between SpaO variants shapes the architecture of the Salmonella type III secretion sorting platform

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AdiY acts as a cytoplasmic pH sensor via histidine protonation to regulate acid stress adaptation in Escherichia coli

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Mapping the architecture of the initiating phosphoglycosyl transferase from S. enterica O-antigen biosynthesis in a liponanoparticle

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DNA/polysome phase separation and cell width confinement couple nucleoid segregation to cell growth in escherichia coli

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Proteolytic activity of surface‐exposed HtrA determines its expression level and is needed to survive acidic conditions in Clostridioides difficile

Corver, J., Claushuis, B., Shamorkina, T. M., de Ru, A. H., van Leeuwen, M. M., Hensbergen, P. J., & Smits, W. K. (2024) · Molecular Microbiology · doi:10.1111/mmi.15300 · Cited by 4

Molecular mechanisms controlling the biogenesis of the TGF-β signal Vg1

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CyanoConstruct: simple platform for cyanobacterial expression construct assembly and translational tuning

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Repurposing endogenous Type I-D CRISPR-Cas system for genome editing in Synechococcus sp. PCC7002

Yang, S., Zhang, Y., Li, C., & Tan, X. (2024) · Microbiological Research · doi:10.1016/j.micres.2024.127884 · Cited by 3

CCSer2 gates dynein activity at the cell periphery

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Improved methodology for protein NMR structure calculation using hydrogen bond restraints and ANSURR validation: The SH2 domain of SH2B1

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Bacillus subtilis RNase HII Is Inefficient at Processing Guanosine Monophosphate and Damaged Ribonucleotides

Cresti, J. R., & Simmons, L. A. (2026) · Molecular Microbiology · doi:10.1111/mmi.70047 · Cited by 2

Robust optogenetic inhibition with red-light-sensitive anion-conducting channelrhodopsins

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The chromokinesin Kid (KIF22) forms a homodimer, moves processively along microtubules and transports double-stranded DNA

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Origins and breadth of pairwise epistasis in an α-helix of β-lactamase TEM-1

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Molecular dissection of PI3Kβ synergistic activation by receptor tyrosine kinases, GβGγ, and Rho-family GTPases

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Targeting mycobacterial transpeptidases: evaluating the roles of Ldt and PBP inhibition in suppressing Mycobacterium smegmatis

de Munnik, M., Calvopiña, K., Rabe, P., & Schofield, C. J. (2025) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00126-25 · Cited by 1

On the Role of VP3-PI3P Interaction in Birnavirus Endosomal Membrane Targeting

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Mapping the architecture of the initiating phosphoglycosyl transferase from S. enterica O-antigen biosynthesis in a liponanoparticle

Dodge, G. J., Anderson, A. J., He, Y., Liu, W., Viner, R., & Imperiali, B. (2023) · doi:10.1101/2023.06.16.545297 · Cited by 1

Fitness landscape of substrate-adaptive mutations in evolved amino acid-polyamine-organocation transporters

Karapanagioti, F., Atlason, Ú. Á., Slotboom, D. J., Poolman, B., & Obermaier, S. (2024) · eLife · doi:10.7554/elife.93971.3 · Cited by 1

Transposon targeting non-coding RNA transcription targets G/C-rich tracts and is facilitated by an intrinsically disordered protein in Tetrahymena

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A novel biosensor for ferrous iron developed via CoBiSe: A computational method for rapid biosensor design

Papadopoulos, A., Anlauf, M. T., Reiners, J., Paik, S.-H., Krüger, A., Lückel, B., Bott, M., Drepper, T., Frunzke, J., Gohlke, H., et al. (2025) · doi:10.1101/2025.07.16.665043

Molecular Mechanism and Structural Models of Protein-Mediated Copper Transfer to the Arabidopsis thaliana Ethylene Receptor ETR1 at the ER Membrane

Dluhosch, D., Kersten, L. S., Minges, A., Schott-Verdugo, S., Gohlke, H., & Groth, G. (2025) · doi:10.1101/2025.01.21.634023

Improved methodology for protein NMR structure calculation using hydrogen bond restraints and ANSURR validation: the SH2 domain of SH2B1

Fowler, N. J., Albalwi, M. F., Lee, S., Hounslow, A. M., & Williamson, M. P. (2022) · doi:10.1101/2022.08.29.505644

A Luciferase-Based Approach for Functional Screening of 5′ and 3′ Untranslated Regions of the mRNA Component for mRNA Vaccines

Rubtsova, M., Mokrushina, Y., Andreev, D., Poteshnova, M., Shepelev, N., Koryagina, M., Moiseeva, E., Malabuiok, D., Prokopenko, Y., Terekhov, S., et al. (2025) · Vaccines · doi:10.3390/vaccines13050530

Molecular dissection of PI3Kβ synergistic activation by receptor tyrosine kinases, GβGγ, and Rho-family GTPases

Duewell, B. R., Wilson, N. E., Bailey, G. M., Peabody, S. E., & Hansen, S. D. (2023) · doi:10.1101/2023.05.01.538969

The chromokinesin Kid (KIF22) forms a homodimer, moves processively along microtubules, and transports double-stranded DNA

Niwa, S., Furusaki, N., Kita, T., Suzuki, Y., & Chiba, K. (2026) · eLife · doi:10.7554/elife.102828

A protein disulfide isomerase coordinates redox homeostasis and ER calcium regulation for optimal lytic cycle progression in Toxoplasma gondii

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Intraflagellar transport protein IFT172 contains a C-terminal ubiquitin-binding U-box-like domain involved in ciliary signaling

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Fitness landscape of substrate-adaptive mutations in evolved APC transporters

Karapanagioti, F., Atlason, Ú. Á., Slotboom, D. J., Poolman, B., & Obermaier, S. (2023) · doi:10.1101/2023.10.12.562049

The Paulinella chromatophore transit peptide part2 adopts a structural fold similar to the γ-glutamyl-cyclotransferase fold

Klimenko, V., Reiners, J., Applegate, V., Reimann, K., Hoeppner, A., Smits, S. H. J., & Nowack, E. C. M. (2025) · doi:10.1101/2025.01.15.633182