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Bone marrow–on–a–chip replicates hematopoietic niche physiology in vitro
Open accessAbstract
Current in vitro hematopoiesis models fail to demonstrate the cellular diversity and complex functions of living bone marrow; hence, most translational studies relevant to the hematologic system are conducted in live animals. Here we describe a method for fabricating 'bone marrow–on–a–chip' that permits culture of living marrow with a functional hematopoietic niche in vitro by first engineering new bone in vivo, removing it whole and perfusing it with culture medium in a microfluidic device. The engineered bone marrow (eBM) retains hematopoietic stem and progenitor cells in normal in vivo–like proportions for at least 1 week in culture. eBM models organ-level marrow toxicity responses and protective effects of radiation countermeasure drugs, whereas conventional bone marrow culture methods do not. This biomimetic microdevice offers a new approach for analysis of drug responses and toxicities in bone marrow as well as for study of hematopoiesis and hematologic diseases in vitro.
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Metadata
- Authors
- Torisawa, Y.-S., Spina, C. S., Mammoto, T., Mammoto, A., Weaver, J. C., Tat, T., Collins, J. J., & Ingber, D. E.
- Venue
- Nature Methods
- Published
- 2014-05-04
- Volume
- 11
- Issue
- 6
- Pages
- 663-669
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2151151867
- Cited by
- 451
- References
- 37
- FWCI
- 20.36
- Type
- journal article
- Language
- en
- Open access
- green
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