Cited by 269Genomics and Chromatin DynamicsChromosomal and Genetic VariationsChromatin Remodeling and Cancer

Multiplexed analysis of chromosome conformation at vastly improved sensitivity

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Davies, James O J, Telenius, Jelena M, McGowan, Simon J, Roberts, Nigel A, Taylor, Stephen, Higgs, Douglas R, Hughes, Jim R·2016·Nature Methods

Abstract

Methods for analyzing chromosome conformation in mammalian cells are either low resolution or low throughput and are technically challenging. In next-generation (NG) Capture-C, we have redesigned the Capture-C method to achieve unprecedented levels of sensitivity and reproducibility. NG Capture-C can be used to analyze many genetic loci and samples simultaneously. High-resolution data can be produced with as few as 100,000 cells, and single-nucleotide polymorphisms can be used to generate allele-specific tracks. The method is straightforward to perform and should greatly facilitate the investigation of many questions related to gene regulation as well as the functional dissection of traits examined in genome-wide association studies.

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Authors
Davies, J. O. J., Telenius, J. M., McGowan, S. J., Roberts, N. A., Taylor, S., Higgs, D. R., & Hughes, J. R.
Affiliations
University of Oxford (GB)
Corresponding author
Hughes, Jim R
Venue
Nature Methods
Published
2015-11-23
Volume
13
Issue
1
Pages
74-80
ISSN
  • 1548-7091 (ISSN-L)
  • 1548-7105
OpenAlex
W2173155483
Cited by
268
References
35
Citations by year
2026: 11 · 2025: 11 · 2024: 24 · 2023: 25 · 2022: 26 · 2021: 29 · 2020: 41 · 2019: 37 · 2018: 29 · 2017: 19 · 2016: 15 · 2015: 1
FWCI
9.27
Type
journal article
Language
en
Open access
Locations
  • Nature Methods (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
Wellcome Trust (098931/Z/12/Z, 106130/Z/14/Z, 106130)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
Keywords
chromosome conformation, 3C, single nucleotide polymorphisms, gene expression regulation, genome-wide association study
MeSH terms
Chromosomes, Human, Humans, Regulatory Sequences, Nucleic Acid, Reproducibility of Results, Polymorphism, Single Nucleotide

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