Cited by 833CRISPR and Genetic EngineeringBacterial Genetics and BiotechnologyRNA regulation and disease

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Beyond editing: repurposing CRISPR-Cas9 for precision genome regulation and interrogation

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Dominguez, Antonia A., Lim, Wendell A., Qi, Lei S.·2016·Nature Reviews Molecular Cell Biology

DOI 10.1038/nrm.2015.2PMC PMC4922510

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Abstract

The bacterial CRISPR-Cas9 system has emerged as a multifunctional platform for sequence-specific regulation of gene expression. This Review describes the development of technologies based on nuclease-deactivated Cas9, termed dCas9, for RNA-guided genomic transcription regulation, both by repression through CRISPR interference (CRISPRi) and by activation through CRISPR activation (CRISPRa). We highlight different uses in diverse organisms, including bacterial and eukaryotic cells, and summarize current applications of harnessing CRISPR-dCas9 for multiplexed, inducible gene regulation, genome-wide screens and cell fate engineering. We also provide a perspective on future developments of the technology and its applications in biomedical research and clinical studies.

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Authors: Dominguez, A. A., Lim, W. A., & Qi, L. S.
Affiliations: Stanford University (US)
QB3 (US)
Corresponding author: Lim, Wendell A.
Qi, Lei S.
ORCID: Dominguez, Antonia A.
Lim, Wendell A.
Qi, Lei S.
Venue: Nature Reviews Molecular Cell Biology
Publisher: Springer Science and Business Media LLC
Published: 2015-12-16
Volume: 17
Issue: 1
Pages: 5-15
ISSN: 1471-0072 (ISSN-L)
1471-0080
DOI: 10.1038/nrm.2015.2
PMC: PMC4922510
OpenAlex: W2204875887
Cited by: 832
References: 91
Citations by year: 2026: 39 · 2025: 41 · 2024: 58 · 2023: 65 · 2022: 78 · 2021: 81 · 2020: 95 · 2019: 105 · 2018: 106 · 2017: 96 · 2016: 63 · 2015: 2
FWCI: 8.63
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.springer.com/tdm (tdm)
Locations: Nature Reviews Molecular Cell Biology (publishedVersion)
PubMed (publishedVersion)
Europe PMC (PubMed Central) (acceptedVersion)
PubMed Central (acceptedVersion)
eScholarship (California Digital Library) (submittedVersion)
Funders: Howard Hughes Medical Institute
National Institutes of Health (DP5OD017887)
National Institute on Drug Abuse (R01DA036858)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
Keywords: CRISPR/Cas9, dCas9, CRISPR interference, CRISPR activation, gene expression inhibition, gene activation, genome-wide screen
MeSH terms: Animals, Humans, Models, Genetic, Transcription, Genetic, Genome, RNA Editing, Genomics, CRISPR-Cas Systems

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