The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
First-in-human liver-tumour surgery guided by multispectral fluorescence imaging in the visible and near-infrared-I/II windows
Abstract
The second near-infrared wavelength window (NIR-II, 1,000-1,700 nm) enables fluorescence imaging of tissue with enhanced contrast at depths of millimetres and at micrometre-scale resolution. However, the lack of clinically viable NIR-II equipment has hindered the clinical translation of NIR-II imaging. Here, we describe an optical-imaging instrument that integrates a visible multispectral imaging system with the detection of NIR-II and NIR-I (700-900 nm in wavelength) fluorescence (by using the dye indocyanine green) for aiding the fluorescence-guided surgical resection of primary and metastatic liver tumours in 23 patients. We found that, compared with NIR-I imaging, intraoperative NIR-II imaging provided a higher tumour-detection sensitivity (100% versus 90.6%; with 95% confidence intervals of 89.1%-100% and 75.0%-98.0%, respectively), a higher tumour-to-normal-liver-tissue signal ratio (5.33 versus 1.45) and an enhanced tumour-detection rate (56.41% versus 46.15%). We infer that combining the NIR-I/II spectral windows and suitable fluorescence probes might improve image-guided surgery in the clinic.
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“Compounding this issue, significant liver accumulation of these dyes generates intense background fluorescence, further compromising imaging accuracy66.”
From Organic small-molecule NIR-II fluorophores for tumor phototheranostics (2026) · cites this paper as [66]
Citations
Cites
Near-infrared fluorophores for biomedical imaging
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A small-molecule dye for NIR-II imaging
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Image-guided cancer surgery using near-infrared fluorescence
Vahrmeijer, A. L., Hutteman, M., van der Vorst, J. R., van de Velde, C. J. �., & Frangioni, J. V. (2013) · Nature Reviews Clinical Oncology · doi:10.1038/nrclinonc.2013.123 · Cited by 1,507
A Molecular Imaging Primer: Modalities, Imaging Agents, and Applications
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Self-citationHe, S., Song, J., Qu, J., & Cheng, Z. (2018) · Chemical Society Reviews · doi:10.1039/c8cs00234g · Cited by 1,021
Self-citationMultifunctional in vivo vascular imaging using near-infrared II fluorescence
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Real‐time identification of liver cancers by using indocyanine green fluorescent imaging
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A high quantum yield molecule-protein complex fluorophore for near-infrared II imaging
Antaris, A. L., Chen, H., Diao, S., Ma, Z., Zhang, Z., Zhu, S., Wang, J., Lozano, A. X., Fan, Q., Chew, L., et al. (2017) · Nature Communications · doi:10.1038/ncomms15269 · Cited by 623
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Advanced Fluorescence Imaging Technology in the Near-Infrared-II Window for Biomedical Applications
Li, C., Chen, G., Zhang, Y., Wu, F., & Wang, Q. (2020) · Journal of the American Chemical Society · doi:10.1021/jacs.0c07022 · Cited by 1,037
Self-citationX-ray-activated persistent luminescence nanomaterials for NIR-II imaging
Pei, P., Chen, Y., Sun, C., Fan, Y., Yang, Y., Liu, X., Lu, L., Zhao, M., Zhang, H., Zhao, D., et al. (2021) · Nature Nanotechnology · doi:10.1038/s41565-021-00922-3 · Cited by 718
Self-citationMolecular Engineering of NIR‐II Fluorophores for Improved Biomedical Detection
Lei, Z., & Zhang, F. (2020) · Angewandte Chemie International Edition · doi:10.1002/anie.202007040 · Cited by 699
Near-infrared luminescence high-contrast in vivo biomedical imaging
Chen, Y., Wang, S., & Zhang, F. (2023) · Nature Reviews Bioengineering · doi:10.1038/s44222-022-00002-8 · Cited by 615
Self-citationIn vivo NIR-II fluorescence imaging for biology and medicine
Wang, F., Zhong, Y., Bruns, O., Liang, Y., & Dai, H. (2024) · Nature Photonics · doi:10.1038/s41566-024-01391-5 · Cited by 564
Metadata
- Authors
- Hu, Z., Fang, C., Li, B., Zhang, Z., Cao, C., Cai, M., Su, S., Sun, X., Shi, X., Li, C., Zhou, T., Zhang, Y., Chi, C., He, P., Xia, X., Chen, Y., Gambhir, S. S., Cheng, Z., & Tian, J.
- Affiliations
- Chinese Academy of Sciences (CN)
- Southwest Medical University (CN)
- Xidian University (CN)
- Stanford Medicine (US)
- Corresponding author
- Gambhir, Sanjiv Sam
- Cheng, Zhen
- Tian, Jie
- ORCID
- Venue
- Nature Biomedical Engineering
- Published
- 2019-12-23
- Volume
- 4
- Issue
- 3
- Pages
- 259-271
- ISSN
- 2157-846X (ISSN-L)
- OpenAlex
- W2995471545
- Cited by
- 1,126
- References
- 38
- Citations by year
- 2026: 120 · 2025: 197 · 2024: 203 · 2023: 206 · 2022: 179 · 2021: 149 · 2020: 72
- FWCI
- 66.02
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Nature Biomedical Engineering (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81930053, 61622117, 81671759, 81227901)
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Optical Imaging and Spectroscopy Techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
- Keywords
- NIR-II fluorescence imaging, multispectral imaging, fluorescence-guided surgery, liver tumors, indocyanine green, cancer detection
- MeSH terms
- Animals, Disease Models, Animal, Female, Fluorescence, Carcinoma, Hepatocellular, Humans, Indocyanine Green, Infrared Rays, Liver, Liver Neoplasms, Male, Surgery, Computer-Assisted, Mice, Hep G2 Cells, Optical Imaging
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