Circuit and molecular architecture of a ventral hippocampal network
Open accessAbstract
The ventral hippocampus (vHPC) is a critical hub in networks that process emotional information. While recent studies have indicated that ventral CA1 (vCA1) projection neurons are functionally dissociable, the basic principles of how the inputs and outputs of vCA1 are organized remain unclear. Here, we used viral and sequencing approaches to define the logic of the extended vCA1 circuit. Using high-throughput sequencing of genetically barcoded neurons (MAPseq) to map the axonal projections of thousands of vCA1 neurons, we identify a population of neurons that simultaneously broadcast information to multiple areas known to regulate the stress axis and approach-avoidance behavior. Through molecular profiling and viral input-output tracing of vCA1 projection neurons, we show how neurons with distinct projection targets may differ in their inputs and transcriptional signatures. These studies reveal new organizational principles of vCA1 that may underlie its functional heterogeneity.
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This predicted isocortex-like circuit organization is highly consistent with currently known interareal connections of HPF, providing validation to the transcriptomic cell type framework.
“This prediction is highly consistent with the currently known interareal connections of HPF, providing a level of validation to the transcriptomic cell type framework”
From A taxonomy of transcriptomic cell types across the isocortex and hippocampal formation (2021) · cites this paper as Gergues et al., 2020
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A taxonomy of transcriptomic cell types across the isocortex and hippocampal formation
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- Authors
- Gergues, M. M., Han, K. J., Choi, H. S., Brown, B., Clausing, K. J., Turner, V. S., Vainchtein, I. D., Molofsky, A. V., & Kheirbek, M. A.
- Affiliations
- University of California, San Francisco (US)
- Harvard University (US)
- Corresponding author
- Kheirbek, Mazen A.
- ORCID
- Venue
- Nature Neuroscience
- Published
- 2020-09-14
- Volume
- 23
- Issue
- 11
- Pages
- 1444-1452
- ISSN
- 1097-6256 (ISSN-L)
- 1546-1726
- PMC
- PMC7606799
- OpenAlex
- W3086890576
- Cited by
- 158
- References
- 54
- Citations by year
- 2026: 21 · 2025: 24 · 2024: 41 · 2023: 25 · 2022: 24 · 2021: 18 · 2020: 4 · 2019: 1
- FWCI
- 9.54
- Type
- journal article
- Language
- en
- Update notices
- correction (2021-09-22)
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Nature Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- U.S. Department of Health & Human Services | NIH | National Institute of Mental Health (R01 MH111754, R01 MH117961)
- Topics
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Keywords
- ventral hippocampus, ventral CA1, projection neurons, neural circuit organization, axonal projections, stress axis, approach-avoidance behavior, emotional processing, gene expression signatures, neuronal heterogeneity, viral tracing
- MeSH terms
- Animals, Brain, Female, Male, Mice, Inbred C57BL, Neural Pathways, Neurons, Gene Expression Profiling, CA1 Region, Hippocampal
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