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Spatial organization of Pseudomonas aeruginosa biofilms probed by combined matrix-assisted laser desorption ionization mass spectrometry and confocal Raman microscopy
Abstract
The chemical composition of bacterial biofilms is explored and visualized with the combination of two label-free molecular imaging techniques.
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“MALDI guided MSI workflows identify phenazines and other metabolites across depth, allowing true overlays of chemotypes and structure [253,254,255].”
From Understanding Pseudomonas aeruginosa Biofilms: Quorum Sensing, c-di-GMP Signaling, and Emerging Antibiofilm Approaches (2026) · cites this paper as [253]
In the citing paper
“The most informative pipeline now integrates RNA-seq and proteomics with structured imaging such as CLSM and OCT, and chemical maps from Raman and MSI.”
From Understanding Pseudomonas aeruginosa Biofilms: Quorum Sensing, c-di-GMP Signaling, and Emerging Antibiofilm Approaches (2026) · cites this paper as [253]
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Imaging mass spectrometry in microbiology
Watrous, J. D., & Dorrestein, P. C. (2011) · Nature Reviews Microbiology · doi:10.1038/nrmicro2634 · Cited by 338
Label‐Free Live‐Cell Imaging of Nucleic Acids Using Stimulated Raman Scattering Microscopy
Zhang, X., Roeffaers, M. B. J., Basu, S., Daniele, J. R., Fu, D., Freudiger, C. W., Holtom, G. R., & Xie, X. S. (2012) · ChemPhysChem · doi:10.1002/cphc.201100890 · Cited by 171
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Cited by
Harvey, D. J. (2018) · Mass Spectrometry Reviews · doi:10.1002/mas.21530 · Cited by 61
Elbehiry, A., Marzouk, E., Edrees, H. M., Ibrahem, M., Alzahrani, S., Anagreyyah, S., Abualola, H., Alghamdi, A., Alzahrani, A., Jaber, M., et al. (2026) · Microorganisms · doi:10.3390/microorganisms14010109 · Cited by 34
Metadata
- Authors
- Masyuko, R. N., Lanni, E. J., Driscoll, C. M., Shrout, J. D., Sweedler, J. V., & Bohn, P. W.
- Affiliations
- Department of Chemistry and Biochemistry
- Department of Chemistry
- Department of Civil and Environmental Engineering and Earth Sciences
- Venue
- The Analyst
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2014
- Volume
- 139
- Issue
- 22
- Pages
- 5700-5708
- ISSN
- 0003-2654
- 1364-5528
- Cited by
- 45
- References
- 2
- Language
- en
- Update notices
- correction (2014-09-29)
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- Masyuko, R. N., Lanni, E. J., Driscoll, C. M., Shrout, J. D., Sweedler, J. V., & Bohn, P. W. (2014). Spatial organization of Pseudomonas aeruginosa biofilms probed by combined matrix-assisted laser desorption ionization mass spectrometry and confocal Raman microscopy. The Analyst. https://doi.org/10.1039/c4an00435c
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