Point mutant mice with hypersensitive α4 nicotinic receptors show dopaminergic deficits and increased anxiety
Open accessAbstract
Knock-in mice were generated that harbored a leucine-to-serine mutation in the α4 nicotinic receptor near the gate in the channel pore. Mice with intact expression of this hypersensitive receptor display dominant neonatal lethality. These mice have a severe deficit of dopaminergic neurons in the substantia nigra, possibly because the hypersensitive receptors are continuously activated by normal extracellular choline concentrations. A strain that retains the neo selection cassette in an intron has reduced expression of the hypersensitive receptor and is viable and fertile. The viable mice display increased anxiety, poor motor learning, excessive ambulation that is eliminated by very low levels of nicotine, and a reduction of nigrostriatal dopaminergic function upon aging. These knock-in mice provide useful insights into the pathophysiology of sustained nicotinic receptor activation and may provide a model for Parkinson's disease.
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Mice with increased activity of α4β2* nAChRs showed increased anxiety-like behaviour.
“Specifically, mice with increased activity of α 4 β 2 * nAChRs showed increased anxiety-like behaviour (Labarca et al., 2001).”
From Age-dependent Effects of Tobacco Smoke and Nicotine on Cognition and the Brain: A Systematic Review of the Human and Animal Literature Comparing Adolescents and Adults. (2023) · cites this paper as Labarca et al., 2001
Lynx1 plays a critical role in maintaining a balance between the beneficial effects of short-term nAChR activation and the potentially devastating degenerative excitotoxic effects of chronic over-activation of nAChRs.
“We also conclude that lynx1 plays a critical role in maintaining a balance between the beneficial effects of short-term nAChR activation (117) and the potentially devastating degenerative excitotoxic effects of chronic over-activation of nAChRs (118,119).”
From Nicotine is a selective pharmacological chaperone of acetylcholine receptor number and stoichiometry. Implications for drug discovery (2009) · cites this paper as [119]
Mice with increased activity of α4β2* nAChRs due to a point mutation in the α4 subunit show increased anxiety-like behaviors.
“mice with increased activity of α4β2* nAChRs as a result of a point mutation in the α4 subunit show increased anxiety-like behaviors (Labarca et al., 2001)”
From Mood and anxiety regulation by nicotinic acetylcholine receptors: A potential pathway to modulate aggression and related behavioral states (2015) · cites this paper as Labarca et al., 2001
α4 knockout mice showed increased anxiety in the elevated plus maze.
“α4 KO mice showed increased anxiety in the same paradigm (Labarca et al., 2001; Ross et al., 2000).”
From Nicotine addiction and psychiatric disorders (2015) · cites this paper as Labarca et al., 2001
Alterations in the activation of the a4 nAChR subunit result in heightened anxiety.
“For example, alterations in the activation of the a4 nAChR subunit result in heightened anxiety (Ross et al., 2000;Labarca et al., 2001).”
From Activation of α4β2*/α6β2* nicotinic receptors alleviates anxiety during nicotine withdrawal without upregulating nicotinic receptors (2014) · cites this paper as Labarca et al., 2001
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Cites
The use of a plus-maze to measure anxiety in the mouse
Lister, R. G. (1987) · Psychopharmacology · doi:10.1007/bf00177912 · Cited by 2,617 · see citing works
Preliminary report of a simple animal behavior model for the anxiolytic effects of benzodiazepines
Crawley, J. N., & Goodwin, F. K. (1980) · Pharmacology Biochemistry and Behavior · doi:10.1016/0091-3057(80)90067-2 · Cited by 1,432 · see citing works
Picciotto, M. R., Zoli, M., Rimondini, R., Léna, C., Marubio, L. M., Pich, E. M., Fuxe, K., & Changeux, J.-P. (1998) · Nature · doi:10.1038/34413 · Cited by 1,320 · see citing works
Dopamine Neuron Agenesis in Nurr1-Deficient Mice
Zetterström, R. H., Solomin, L., Jansson, L., Hoffer, B. J., Olson, L., & Perlmann, T. (1997) · Science · doi:10.1126/science.276.5310.248 · Cited by 1,096 · see citing works
Bale, T. L., Contarino, A., Smith, G. W., Chan, R. K. W., Gold, L. H., Sawchenko, P. E., Koob, G. F., Vale, W. W., & Lee, K. (2000) · Nature Genetics · doi:10.1038/74263 · Cited by 850 · see citing works
Zhong, W., Gallivan, J. P., Zhang, Y., Li, L., Lester, H. A., & Dougherty, D. A. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.21.12088 · Cited by 550 · see citing works
Self-citationMutations in the channel domain alter desensitization of a neuronal nicotinic receptor
Revah, F., Bertrand, D., Galzi, J., Devillers‐Thiéry, A., Mulle, C., Hussy, N., Bertrand, S., Ballivet, M., & Changeux, J. (1991) · Nature · doi:10.1038/353846a0 · Cited by 524 · see citing works
Bannon, A. W., Decker, M. W., Holladay, M. W., Curzon, P., Donnelly‐Roberts, D. L., Puttfarcken, P. S., Bitner, R. S., Díaz, Á., Dickenson, A. H., Porsolt, R. D., et al. (1998) · Science · doi:10.1126/science.279.5347.77 · Cited by 368 · see citing works
Li, Y., Zhang, Y., Lester, H. A., Schuman, E. M., & Davidson, N. (1998) · Journal of Neuroscience · doi:10.1523/jneurosci.18-24-10231.1998 · Cited by 318 · see citing works
Self-citationLabarca, C. G., Nowak, M. W., Zhang, H., Tang, L., Deshpande, P., & Lester, H. A. (1995) · Nature · doi:10.1038/376514a0 · Cited by 302 · see citing works
Self-citationMarshall, D. L., Redfern, P. H., & Wonnacott, S. (1997) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1997.68041511.x · Cited by 284 · see citing works
Xu, W., Orr‐Urtreger, A., Nigro, F., Gelber, S. E., Sutcliffe, C. B., Armstrong, D. L., Patrick, J. W., Role, L. W., Beaudet, A. L., & Biasi, M. D. (1999) · Journal of Neuroscience · doi:10.1523/jneurosci.19-21-09298.1999 · Cited by 283 · see citing works
Nicotinic Receptor-Induced Apoptotic Cell Death of Hippocampal Progenitor Cells
Berger, F. R., Gage, F. H., & Vijayaraghavan, S. (1998) · Journal of Neuroscience · doi:10.1523/jneurosci.18-17-06871.1998 · Cited by 233 · see citing works
Smoking and Parkinson’s disease
Gorell, J. M., Rybicki, B. A., Johnson, C. C., & Peterson, E. L. (1999) · Neurology · doi:10.1212/wnl.52.1.115 · Cited by 224 · see citing works
Increased neurodegeneration during ageing in mice lacking high‐affinity nicotine receptors
Zoli, M., Picciotto, M. R., Ferrari, R., Cocchi, D., & Changeux, J. (1999) · The EMBO Journal · doi:10.1093/emboj/18.5.1235 · Cited by 206 · see citing works
Ross, S. A., Wong, J. Y. F., Clifford, J. J., Kinsella, A., Massalas, J. S., Horne, M. K., Scheffer, I. E., Kola, I., Waddington, J. L., Berkovic, S. F., et al. (2000) · The Journal of Neuroscience · doi:10.1523/jneurosci.20-17-06431.2000 · Cited by 204 · see citing works
File, S. E., Kenny, P. J., & Ouagazzal, A. (1998) · Behavioral Neuroscience · doi:10.1037//0735-7044.112.6.1423 · Cited by 181 · see citing works
The role of conserved leucines in the M2 domain of the acetylcholine receptor in channel gating.
Filatov, G., & White, M. M. (1995) · Molecular Pharmacology · doi:10.1016/s0026-895x(25)10484-7 · Cited by 177 · see citing works
Nowak, M. W., Gallivan, J. P., Silverman, S. K., Labarca, C. G., Dougherty, D. A., & Lester, H. A. (1998) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(98)93031-2 · Cited by 169 · see citing works
Self-citationCharpantier, E., Barnéoud, P., Moser, P. C., Besnard, F., & Sgard, F. (1998) · Neuroreport · doi:10.1097/00001756-199809140-00033 · Cited by 166 · see citing works
File, S. E., Kenny, P. J., & Ouagazzal, A. (1998) · Behavioral Neuroscience · doi:10.1037/0735-7044.112.6.1423 · Cited by 166 · see citing works
Nicotine psychopharmacology; molecular, cellular and behavioural aspects
Wonnacott, S. (1991) · General Pharmacology The Vascular System · doi:10.1016/0306-3623(91)90259-9 · Cited by 148 · see citing works
Cytokine-induced conversion of mesencephalic-derived progenitor cells into dopamine neurons
Potter, E. D., Ling, Z. D., & Carvey, P. M. (1999) · Cell and Tissue Research · doi:10.1007/s004410051285 · Cited by 141 · see citing works
Orr‐Urtreger, A., Broide, R. S., Kasten, M. R., Dang, H., Dani, J. A., Beaudet, A. L., & Patrick, J. W. (2000) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2000.0742154.x · Cited by 129 · see citing works
Self-citationKlein, J., Gonzalez, R., Köppen, A., et al. (1993) · Neurochemistry International · doi:10.1016/0197-0186(93)90058-d · Cited by 113 · see citing works
Arroyo-Jiménez, M. D. M., Bourgeois, J.-P., Marubio, L. M., Le Sourd, A.-M., Ottersen, O. P., Rinvik, E., Fairén, A., & Changeux, J.-P. (1999) · The Journal of Neuroscience · doi:10.1523/jneurosci.19-15-06475.1999 · Cited by 110 · see citing works
Latency to enter a mirrored chamber: A novel behavioral assay for anxiolytic agents
Toubas, P. L., Abla, K. A., Cao, W., et al. (1990) · Pharmacology Biochemistry and Behavior · doi:10.1016/0091-3057(90)90215-4 · Cited by 87 · see citing works
Smoking and Parkinson's disease: a case-control study in Germany
Hellenbrand, W., Seidler, A., Robra, B., Vieregge, P., Oertel, W. H., Joerg, J., NISCHAN, P., Schneider, E., & Ulm, G. (1997) · International Journal of Epidemiology · doi:10.1093/ije/26.2.328 · Cited by 87 · see citing works
Zhou, M., Engel, A. G., & Auerbach, A. L. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.18.10466 · Cited by 85 · see citing works
Dysfunctions in Mice by NMDA Receptor Point Mutations NR1(N598Q) and NR1(N598R)
Single, F. N., Rozov, A. V., Burnashev, N., Zimmermann, F., Hanley, D. F., Forrest, D., Curran, T., Jensen, V., Hvalby, Ø., Sprengel, R., et al. (2000) · Journal of Neuroscience · doi:10.1523/jneurosci.20-07-02558.2000 · Cited by 76 · see citing works
Paradoxical allosteric effects of competitive inhibitors on neuronal α7 nicotinic receptor mutants
Bertrand, S., Devillers‐Thiéry, A., Palma, E., Buisson, B., Edelstein, S. J., Corringer, P., Changeux, J., & Bertrand, D. (1997) · Neuroreport · doi:10.1097/00001756-199711100-00034 · Cited by 70 · see citing works
Mice Lacking PKCγ Exhibit Decreased Anxiety
Bowers, B. J., Collins, A. C., Tritto, T., & Wehner, J. M. (2000) · Behavior Genetics · doi:10.1023/a:1001951104208 · Cited by 61 · see citing works
Self-citationYoung, E. A., Owen, E. H., Meiri, K. F., & Wehner, J. M. (2000) · Brain Research · doi:10.1016/s0006-8993(00)02021-7 · Cited by 50 · see citing works
Self-citationEffects of SCH23390 and raclopride on anxiety-like behavior in rats tested in the black-white box.
Timothy, C., Costall, B., & Smythe, J. W. (1999) · Pharmacology, Biochemistry and Behavior · doi:10.1016/s0091-3057(98)00157-9 · Cited by 48 · see citing works
Benzodiazepine receptor mediation of the anxiolytic-like effect of (−)-nicotine in mice
O'Neill, A. B., & Brioni, J. D. (1994) · Pharmacology Biochemistry and Behavior · doi:10.1016/0091-3057(94)90097-3 · Cited by 38 · see citing works
Picciotto, M. R., Zoli, M., Zachariou, V., & Changeux, J. (1997) · Biochemical Society Transactions · doi:10.1042/bst0250824 · Cited by 35 · see citing works
Meshul, C. K., Buckman, J. F., Allen, C., Riggan, J. P., & Feller, D. J. (1996) · Synapse · doi:10.1002/(sici)1098-2396(199604)22:4 · Cited by 30 · see citing works
Characterization of nicotine receptor sites in the brain
Wonnacott, S. (1990) · doi:10.1093/oso/9780192616142.003.0007 · Cited by 21 · see citing works
Flagged first, then by citations
Research works printed but not linked: 4
- [4]4. Wonnacott, S., Russell, M. A. H. & Stolerman, I. P. (Oxford Univ. Press, Oxford), pp. 226-277.
- [5]5. Picciotto, M. R., Zoli, M., Zachariou, V. & Changeux, J.-P. (1997) Biochem. Soc. Trans. 25, 824-829.
- [25]25. Zhong, W., Gallivan, J., Zhang, Y., Li, L., Lester, H. & Dougherty, D. (1998) Proc. Natl. Acad. Sci. USA 95, 12088-12093.
- [33]33. Zhou, M., Engel, A. G. & Auerbach, A. (1999) Proc. Natl. Acad. Sci. USA 96, 10466-10471.
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Tapper, A. R., McKinney, S. L., Nashmi, R., Schwarz, J., Deshpande, P., Labarca, C., Whiteaker, P., Marks, M. J., Collins, A. C., & Lester, H. A. (2004) · Science · doi:10.1126/science.1099420 · Cited by 686 · see citing works
Self-citationThe dynamic effects of nicotine on the developing brain
Dwyer, J. B., McQuown, S. C., & Leslie, F. M. (2009) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2009.02.003 · Cited by 559 · see citing works
Effect of nicotine and nicotinic receptors on anxiety and depression
Picciotto, M. R., Brunzell, D. H., & Caldarone, B. J. (2002) · Neuroreport · doi:10.1097/00001756-200207020-00006 · Cited by 483 · see citing works
Nicotine addiction and nicotinic receptors: lessons from genetically modified mice
Changeux, J.-P. (2010) · Nature Reviews Neuroscience · doi:10.1038/nrn2849 · Cited by 453 · see citing works
Nashmi, R., Xiao, C., Deshpande, P., McKinney, S., Grady, S. R., Whiteaker, P., Huang, Q., McClure-Begley, T., Lindstrom, J. M., Labarca, C., et al. (2007) · The Journal of Neuroscience · doi:10.1523/jneurosci.2199-07.2007 · Cited by 274 · see citing works
Self-citationNicotine and brain development
Dwyer, J. B., Broide, R. S., & Leslie, F. M. (2008) · Birth Defects Research Part C: Embryo Today: Reviews · doi:10.1002/bdrc.20118 · Cited by 256 · see citing works
Nicotine as a modulator of behavior: beyond the inverted U
Picciotto, M. R. (2003) · Trends in Pharmacological Sciences · doi:10.1016/s0165-6147(03)00230-x · Cited by 257 · see citing works
Mozhui, K., Karlsson, R.-M., Kash, T. L., Ihne, J., Norcross, M., Patel, S., Farrell, M. R., Hill, E. E., Graybeal, C., Martin, K. P., et al. (2010) · The Journal of Neuroscience · doi:10.1523/jneurosci.5017-09.2010 · Cited by 226 · see citing works
Varenicline improves mood and cognition during smoking abstinence
Patterson, F., Jepson, C., Strasser, A. A., Loughead, J., Perkins, K. A., Gur, R. C., Frey, J. M., Siegel, S., & Lerman, C. (2009) · Biological Psychiatry · doi:10.1016/j.biopsych.2008.08.028 · Cited by 222 · see citing works
Picciotto, M. R., Caldarone, B. J., Brunzell, D. H., Zachariou, V., Stevens, T. R., & King, S. L. (2001) · Pharmacology & Therapeutics · doi:10.1016/s0163-7258(01)00161-9 · Cited by 221 · see citing works
Drenan, R. M., Grady, S. R., Whiteaker, P., McClure-Begley, T., McKinney, S., Miwa, J. M., Bupp, S., Heintz, N., McIntosh, J. M., Bencherif, M., et al. (2008) · Neuron · doi:10.1016/j.neuron.2008.09.009 · Cited by 197 · see citing works
Self-citationRoles of Accessory Subunits in α4β2* Nicotinic Receptors
Kuryatov, A., Onksen, J., & Lindstrom, J. (2008) · Molecular Pharmacology · doi:10.1124/mol.108.046789 · Cited by 195 · see citing works
McGranahan, T. M., Patzlaff, N. E., Grady, S. R., Heinemann, S. F., & Booker, T. K. (2011) · Journal of Neuroscience · doi:10.1523/jneurosci.0937-11.2011 · Cited by 165 · see citing works
Lester, H. A., Xiao, C., Srinivasan, R., Son, C. D., Miwa, J., Pantoja, R., Banghart, M. R., Dougherty, D. A., Goate, A. M., & Wang, J. C. (2009) · The AAPS Journal · doi:10.1208/s12248-009-9090-7 · Cited by 163 · see citing works
Self-citationPicciotto, M. R., Lewis, A. S., van Schalkwyk, G. I., & Mineur, Y. S. (2015) · Neuropharmacology · doi:10.1016/j.neuropharm.2014.12.028 · Cited by 157 · see citing works
Miwa, J. M., Stevens, T. R., King, S. L., Caldarone, B. J., Ibanez-Tallon, I., Xiao, C., Fitzsimonds, R. M., Pavlides, C., Lester, H. A., Picciotto, M. R., et al. (2006) · Neuron · doi:10.1016/j.neuron.2006.07.025 · Cited by 154 · see citing works
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Developmental regulation of nicotinic acetylcholine receptors within midbrain dopamine neurons
Azam, L., Chen, Y., & Leslie, F. M. (2007) · Neuroscience · doi:10.1016/j.neuroscience.2006.11.011 · Cited by 138 · see citing works
Fowler, C. D., Arends, M. A., & Kenny, P. J. (2008) · Behavioural Pharmacology · doi:10.1097/fbp.0b013e32830c360e · Cited by 131 · see citing works
Role of nicotinic acetylcholine receptors in regulating dopamine neuron activity
Faure, P., Tolu, S., Valverde, S., & Naudé, J. (2014) · Neuroscience · doi:10.1016/j.neuroscience.2014.05.040 · Cited by 125 · see citing works
Nicotine modulation of fear memories and anxiety: Implications for learning and anxiety disorders
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Nicotinic acetylcholine receptors in neurological and psychiatric diseases
Terry, A. V., Jones, K., & Bertrand, D. (2023) · Pharmacological Research · doi:10.1016/j.phrs.2023.106764 · Cited by 90 · see citing works
Progesterone modulation of α5 nAChR subunits influences anxiety‐related behavior during estrus cycle
Gangitano, D., Salas, R., Teng, Y., Perez, E., & De Biasi, M. (2009) · Genes, Brain and Behavior · doi:10.1111/j.1601-183x.2009.00476.x · Cited by 76 · see citing works
Salas, R., Main, A., Gangitano, D., & De Biasi, M. (2007) · Neuropharmacology · doi:10.1016/j.neuropharm.2007.08.017 · Cited by 76 · see citing works
Nicotine addiction and psychiatric disorders
Kutlu, M. G., Parikh, V., & Gould, T. J. (2015) · International Review of Neurobiology · doi:10.1016/bs.irn.2015.08.004 · Cited by 65 · see citing works
Colyer-Patel, K., Kuhns, L., Weidema, A., Lesscher, H., & Cousijn, J. (2023) · Neuroscience & Biobehavioral Reviews · doi:10.1016/j.neubiorev.2023.105038 · Cited by 54 · see citing works
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Booker, T. K., Butt, C. M., Wehner, J. M., Heinemann, S. F., & Collins, A. C. (2007) · Pharmacology Biochemistry and Behavior · doi:10.1016/j.pbb.2007.04.011 · Cited by 49 · see citing works
Self-citationBriggs, C. A., Gubbins, E. J., Marks, M. J., Putman, C. B., Thimmapaya, R., Meyer, M. D., & Surowy, C. S. (2006) · Molecular Pharmacology · doi:10.1124/mol.105.020198 · Cited by 33 · see citing works
Changeux, J.-P. (2006) · Philosophical Transactions of the Royal Society B: Biological Sciences · doi:10.1098/rstb.2006.1832 · Cited by 31 · see citing works
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Kishi, M., & Steinbach, J. H. (2006) · Molecular Pharmacology · doi:10.1124/mol.106.029298 · Cited by 31 · see citing works
Lin, H.-C., Pan, H.-C., Lin, S.-H., Lo, Y.-C., Shen, E. T.-H., Liao, L.-D., Liao, P.-H., Chien, Y.-W., Liao, K.-D., Jaw, F.-S., et al. (2016) · Frontiers in Neural Circuits · doi:10.3389/fncir.2015.00087 · Cited by 29 · see citing works
Yohn, N. L., Turner, J. R., & Blendy, J. A. (2014) · The Journal of Pharmacology and Experimental Therapeutics · doi:10.1124/jpet.113.211706 · Cited by 28 · see citing works
- Authors
- Labarca, C., Schwarz, J., Deshpande, P., Schwarz, S., Nowak, M. W., Fonck, C., Nashmi, R., Kofuji, P., Dang, H., Shi, W., Fidan, M., Khakh, B. S., Chen, Z., Bowers, B. J., Boulter, J., Wehner, J. M., & Lester, H. A.
- Affiliations
- California Institute of Technology (US)
- Corresponding author
- Schwarz, Johannes
- Deshpande, Purnima
- Lester, Henry A.
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2001-02-20
- Volume
- 98
- Issue
- 5
- Pages
- 2786-2791
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC30217
- OpenAlex
- W2073702980
- Cited by
- 177
- References
- 38
- Citations by year
- 2023: 4 · 2022: 2 · 2021: 2 · 2020: 3 · 2019: 2 · 2018: 6 · 2017: 6 · 2016: 7 · 2015: 12 · 2014: 6 · 2013: 2 · 2012: 7
- FWCI
- 5.35
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- CaltechAUTHORS (California Institute of Technology) (submittedVersion)
- Funders
- Alexander von Humboldt-Stiftung
- Tobacco-Related Disease Research Program
- Wellcome Trust
- National Institutes of Health (5t01mh019176-03, -10156, NS-1 1756, 5p01da010156-05, 5r03da011836-02)
- National Institute of Mental Health (MH-19176)
- National Institute on Drug Abuse (DA-11836, P50DA005010, DA10156)
- National Institute of Neurological Disorders and Stroke (NS 11756, R01NS011756)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
- Health Sciences › Medicine › Neurology › Parkinson's Disease Mechanisms and Treatments
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- knock-in mice, neonatal lethality, dopaminergic neurons, substantia nigra, choline, anxiety, motor learning, ambulation, nicotine, nigrostriatal pathway, Parkinson's disease model, aging
- MeSH terms
- Animals, Anxiety, Dopamine, Female, Heterozygote, Immunohistochemistry, Mice, Mutant Strains, Pregnancy, Receptors, Nicotinic, Point Mutation, Mice, Rats
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