UV irradiation induces a postreplication DNA damage checkpoint
Open accessAbstract
Eukaryotic cells irradiated with high doses of UV exhibit cell-cycle responses referred to as G 1 /S, intraS, and G 2 /M checkpoints. After a moderate UV dose that approximates sunlight exposure and is lethal to fission yeast checkpoint mutants, we found unexpectedly that these cell-cycle responses do not occur. Instead, cells at all stages of the cell cycle carry lesions into S phase and delay cell-cycle progression for hours after the completion of bulk DNA synthesis. Both DNA replication and the checkpoint kinase, Chk1, are required to generate this cell-cycle response. UV-irradiation of Δ chk1 cells causes chromosome damage and loss of viability only after cells have replicated irradiated DNA and entered mitosis. These data suggest that an important physiological role of the cell-cycle response to UV is to provide time for postreplication repair.
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Very low UV doses do not appear to activate the G1 checkpoint.
“This is consistent with the observation that very low UV doses do not seem to activate the G1 checkpoint [61,64,65]”
From Mind the gap: keeping UV lesions in check (2011) · cites this paper as [64]
In wild-type yeast, after acute low-dose UVC (5 J/m2), cells do not delay cell cycle progression until they pass through S phase; this is called the post-replication checkpoint.
“In a wild type background, elegant time lapse experiments showed that after an acute low dose of UVC light (5 J/m2) yeast cells do not delay cell cycle progression until they proceed through S phase; for this reason this response was called post-replication checkpoint [64].”
From Mind the gap: keeping UV lesions in check (2011) · cites this paper as [64]
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Postreplication gaps at UV lesions are signals for checkpoint activation
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Self-citationMind the gap: keeping UV lesions in check
Novarina, D., Amara, F., Lazzaro, F., Plevani, P., & Muzi-Falconi, M. (2011) · DNA Repair · doi:10.1016/j.dnarep.2011.04.030 · Cited by 43 · see citing works
“This is consistent with the bservation that very low UV doses do not seem to activate the 1 checkpoint [61,64,65]; if NER can rapidly and effectively deal ith a low number of lesions, there would be no point in triggerng a checkpoint response.”
BackgroundMyelodysplastic syndrome: An inability to appropriately respond to damaged DNA?
Zhou, T., Hasty, P., Walter, C. A., Bishop, A. J. R., Scott, L. M., & Rebel, V. I. (2013) · Experimental Hematology · doi:10.1016/j.exphem.2013.04.008 · Cited by 39 · see citing works
“DNA damage, replication and spindle checkpoints [36, 37] operate to ensure that each stage of the cell cycle has been completed faithfully before proceeding to the next.”
BackgroundWigan, M., Pinder, A., Giles, N., Pavey, S., Burgess, A., Wong, S., Sturm, R. A., & Gabrielli, B. (2012) · Journal of Investigative Dermatology · doi:10.1038/jid.2012.41 · Cited by 17 · see citing works
- Authors
- Callegari, A. J., & Kelly, T. J.
- Affiliations
- Memorial Sloan Kettering Cancer Center (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2006-10-24
- Volume
- 103
- Issue
- 43
- Pages
- 15877-15882
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1998408422
- Cited by
- 51
- References
- 68
- Citations by year
- 2026: 1 · 2023: 2 · 2022: 3 · 2021: 1 · 2020: 1 · 2019: 2 · 2018: 6 · 2017: 2 · 2016: 1 · 2015: 3 · 2014: 1 · 2013: 5 · 2012: 4
- FWCI
- 1.49
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- UV irradiation, DNA damage checkpoint, cell cycle checkpoints, Chk1, DNA replication, S phase, cell cycle arrest, chromosomal damage, Schizosaccharomyces pombe, DNA repair
- MeSH terms
- Checkpoint Kinase 1, Cell Cycle, DNA Damage, DNA Replication, DNA, Fungal, Mutation, Protein Kinases, Schizosaccharomyces, Ultraviolet Rays, Schizosaccharomyces pombe Proteins
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