Discontinuities in the DNA synthesized in an excision-defective strain of Escherichia coli following ultraviolet irradiation
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Blocked polymerases can leave the lesion and PCNA behind and re-initiate downstream via re-priming, generating numerous ssDNA gaps behind forks that likely cause strong Mec1 activation during S phase in UV-irradiated cells.
“Blocked polymerase can leave the lesion and PCNA behind and re-initiate downstream of the lesion via a re-priming mechanism, generating numerous ssDNA gaps behind the forks, which are likely responsible for the strong activation of Mec1 during S phase in UV-irradiated cells [51,82–85].”
From Mind the gap: keeping UV lesions in check (2011) · cites this paper as [82]
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- Authors
- Rupp, W. D., & Howard-flanders, P.
- Affiliations
- Yale University (US)
- Venue
- Journal of Molecular Biology
- Publisher
- Elsevier BV
- Published
- 1968-01
- Volume
- 31
- Issue
- 2
- Pages
- 291-304
- ISSN
- 0022-2836 (ISSN-L)
- OpenAlex
- W1984459901
- Cited by
- 1,093
- References
- 18
- Citations by year
- 2026: 6 · 2025: 6 · 2024: 3 · 2023: 9 · 2022: 4 · 2021: 11 · 2020: 6 · 2019: 18 · 2018: 11 · 2017: 13 · 2016: 9 · 2015: 8 · 2014: 15 · 2013: 15 · 2012: 15
- FWCI
- 29.73
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Molecular Biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- pyrimidine dimers, UV irradiation, DNA synthesis, excision repair, Escherichia coli, DNA gaps, DNA molecular weight, 3H-thymidine incorporation
- MeSH terms
- Centrifugation, Density Gradient, Centrifugation, Zonal, Cesium, Chlorides, Coliphages, DNA, Bacterial, Escherichia coli, Models, Chemical, Molecular Weight, Pyrimidines, Radiation Effects, Sucrose, Thymidine, Tritium, Ultraviolet Rays
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