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Direct isolation of human central nervous system stem cells
Open accessAbstract
Stem cells, which are clonogenic cells with self-renewal and multilineage differentiation properties, have the potential to replace or repair damaged tissue. We have directly isolated clonogenic human central nervous system stem cells (hCNS-SC) from fresh human fetal brain tissue, using antibodies to cell surface markers and fluorescence-activated cell sorting. These hCNS-SC are phenotypically 5F3 (CD133) + , 5E12 + , CD34 − , CD45 − , and CD24 −/lo . Single CD133 + CD34 − CD45 − sorted cells initiated neurosphere cultures, and the progeny of clonogenic cells could differentiate into both neurons and glial cells. Single cells from neurosphere cultures initiated from CD133 + CD34 − CD45 − cells were again replated as single cells and were able to reestablish neurosphere cultures, demonstrating the self-renewal potential of this highly enriched population. Upon transplantation into brains of immunodeficient neonatal mice, the sorted/expanded hCNS-SC showed potent engraftment, proliferation, migration, and neural differentiation.
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(2003) · PubMed
Metadata
- Authors
- Uchida, N., Buck, D. W., He, D., Reitsma, M. J., Masek, M., Phan, T. V., Tsukamoto, A. S., Gage, F. H., & Weissman, I. L.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2000-12-19
- Volume
- 97
- Issue
- 26
- Pages
- 14720-14725
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2065737043
- Cited by
- 1,849
- References
- 3
- FWCI
- 12.63
- Type
- journal article
- Language
- en
- Open access
- green
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