The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Fidelity and Processivity of Saccharomyces cerevisiae DNA Polymerase η
Open accessAbstract
The yeast RAD30 gene functions in error-free replication of UV-damaged DNA, and RAD30 encodes a DNA polymerase, pol eta, that has the ability to efficiently and correctly replicate past a cis-syn-thymine-thymine dimer in template DNA. To better understand the role of pol eta in damage bypass, we examined its fidelity and processivity on nondamaged DNA templates. Steady-state kinetic analyses of deoxynucleotide incorporation indicate that pol eta has a low fidelity, misincorporating deoxynucleotides with a frequency of about 10(-2) to 10(-3). Also pol eta has a low processivity, incorporating only a few nucleotides before dissociating. We suggest that pol eta's low fidelity reflects a flexibility in its active site rendering it more tolerant of DNA damage, while its low processivity limits its activity to reduce errors.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“These multiplet mutations are likely the consequence of error-prone bypass polymerization during translesion synthesis and are consistent with the processivity of bypass polymerases persisting for several nucleotides after the bypass-requiring blocking lesion (39 -41).”
From Why Cockayne syndrome patients do not get cancer despite their DNA repair deficiency (2016) · cites this paper as [39]
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Enzyme structure and mechanism
Fersht, A. (1977) · Medical Entomology and Zoology · Cited by 2,875
Enzyme structure and mechanism
Elmore, D. T. (1978) · Endeavour · doi:10.1016/0160-9327(78)90081-9 · Cited by 2,713
The XPV (xeroderma pigmentosum variant) gene encodes human DNA polymerase η
Masutani, C., Kusumoto, R., Yamada, A., Dohmae, N., Yokoi, M., Yuasa, M., Araki, M., Iwai, S., Takio, K., & Hanaoka, F. (1999) · Nature · doi:10.1038/21447 · Cited by 1,292
DNA Polymerases: Structural Diversity and Common Mechanisms
Steitz, T. A. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.25.17395 · Cited by 848
Efficient Bypass of a Thymine-Thymine Dimer by Yeast DNA Polymerase, Polη
Johnson, R. E., Prakash, S., & Prakash, L. (1999) · Science · doi:10.1126/science.283.5404.1001 · Cited by 736
Self-citationhRAD30 Mutations in the Variant Form of Xeroderma Pigmentosum
Johnson, R. E., Kondratick, C. M., Prakash, S., & Prakash, L. (1999) · Science · doi:10.1126/science.285.5425.263 · Cited by 732
Self-citationFIDELITY MECHANISMS IN DNA REPLICATION
Echols, H., & Goodman, M. F. (1991) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.60.070191.002401 · Cited by 712
Biochemical Basis of DNA Replication Fidelity
Goodman, M. F., Creighton, S., Bloom, L. B., Petruska, J., & Kunkel, T. A. (1993) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.3109/10409239309086792 · Cited by 446
McDonald, J. P., Levine, A. S., & Woodgate, R. (1997) · Genetics · doi:10.1093/genetics/147.4.1557 · Cited by 314
Bailly, V., Lauder, S., Prakash, S., & Prakash, L. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.37.23360 · Cited by 292
Self-citation
Flagged first, then by citations
Research works printed but not linked: 1
- Wang, 1993, Mol. Cell. Biol., 13, 4276
Cited by
Sorted by citations
EUKARYOTIC TRANSLESION SYNTHESIS DNA POLYMERASES: Specificity of Structure and Function
Prakash, S., Johnson, R. E., & Prakash, L. (2005) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.74.082803.133250 · Cited by 1,020
Self-citationRNA-catalyzed RNA polymerization: Accurate and general RNA-templated primer extension.
Johnston, W. K., Unrau, P. J., Lawrence, M. S., Glasner, M. E., & Bartel, D. P. (2001) · Science · doi:10.1126/science.1060786 · Cited by 768
Error-Prone Repair DNA Polymerases in Prokaryotes and Eukaryotes
Goodman, M. F. (2002) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.71.083101.124707 · Cited by 727
Eukaryotic polymerases ι and ζ act sequentially to bypass DNA lesions
Johnson, R. E., Washington, M. T., Haracska, L., Prakash, S., & Prakash, L. (2000) · Nature · doi:10.1038/35023030 · Cited by 632
Self-citationEukaryotic Translesion Polymerases and Their Roles and Regulation in DNA Damage Tolerance
Waters, L. S., Minesinger, B. K., Wiltrout, M. E., D'Souza, S., Woodruff, R. V., & Walker, G. C. (2009) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00034-08 · Cited by 591
Watanabe, K., Tateishi, S., Kawasuji, M., Tsurimoto, T., Inoue, H., & Yamaizumi, M. (2004) · The EMBO Journal · doi:10.1038/sj.emboj.7600383 · Cited by 538
“PolZ synthesizes DNAwith a low fidelity, misincorporating nucleotides with a frequency of 10 2–10 3 (Washington et al, 1999).”
BackgroundRegulation of DNA cross-link repair by the Fanconi anemia/BRCA pathwayPreprint
Kim, H., & D’Andrea, A. D. (2012) · Genes & Development · doi:10.1101/gad.195248.112 · Cited by 534
Mechanisms of accurate translesion synthesis by human DNA polymerase η
Masutani, C., Kusumoto, R., Iwai, S., & Hanaoka, F. (2000) · The EMBO Journal · doi:10.1093/emboj/19.12.3100 · Cited by 504
Low fidelity DNA synthesis by human DNA polymerase-η
Matsuda, T., Bebenek, K., Masutani, C., Hanaoka, F., & Kunkel, T. A. (2000) · Nature · doi:10.1038/35010014 · Cited by 372
Efficient and accurate replication in the presence of 7,8-dihydro-8-oxoguanine by DNA polymerase η
Haracska, L., Yu, S.-L., Johnson, R. E., Prakash, L., & Prakash, S. (2000) · Nature Genetics · doi:10.1038/78169 · Cited by 356
Self-citationTranslesion DNA synthesis in eukaryotes: A one- or two-polymerase affair
Prakash, S., & Prakash, L. (2002) · Genes & Development · doi:10.1101/gad.1009802 · Cited by 344
“…to the high fidelity of replicative polymerases, Pol is a low-fidelity enzyme, and it misincorporates nucleotides with a frequency of ∼10−2 to 10−3 (Washington et al. 1999; Johnson et al. 2000c); in a subsequent study, human Pol was shown to be highly mutagenic in an in vitro DNA synthesis…”
BackgroundSelf-citationStructure of the Catalytic Core of S. cerevisiae DNA Polymerase η
Trincão, J., Johnson, R. E., Escalante, C. R., Prakash, S., Prakash, L., & Aggarwal, A. K. (2001) · Molecular Cell · doi:10.1016/s1097-2765(01)00306-9 · Cited by 348
“; Washington et al., 1999, 2000; Johnson et diffract better and were used for the subsequent structure determial., 2000c; Matsuda et al., 2000).”
MethodSelf-citationTHE SOS RESPONSE: Recent Insights into umuDC -Dependent Mutagenesis and DNA Damage Tolerance
Sutton, M. D., Smith, B. T., Godoy, V. G., & Walker, G. C. (2000) · Annual Review of Genetics · doi:10.1146/annurev.genet.34.1.479 · Cited by 318
“cerevisiae RAD30 -encoded DNA Polη (31) and its human homolog, XP-V or human RAD30A(51, 52), despite being highly error-prone on undamaged templates (53, 90), appear to bypass thyminethymine cyclobutane dimers in a seemingly accurate manner by inserting two As opposite the lesion (31).”
Backgroundpolι, a remarkably error-prone human DNA polymerase
Tissier, A., McDonald, J. P., Frank, E. G., & Woodgate, R. (2000) · Genes & Development · doi:10.1101/gad.14.13.1642 · Cited by 315
Translesion DNA Synthesis and Mutagenesis in Eukaryotes
Sale, J. E. (2013) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a012708 · Cited by 282
“On undamaged DNA, however, Pol h is significantly error prone (Washington et al. 1999; Johnson et al. 2000c; Matsuda et al. 2000; Matsuda et al. 2001).”
BackgroundMolecular Mechanisms of DNA Damage and Repair: Progress in Plants
Tuteja, N., Singh, M. B., Misra, M. K., Bhalla, P. L., & Tuteja, R. (2001) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.1080/20014091074219 · Cited by 272
“The DNA polymerase encoded by yeast RAD30 and human hRAD30A also bypasses a cis-syn thymine-thymine dimer efficiently and accurately (Johnson et al., 1999 a,b; Masutani et al., 1999; Washington et al., 1999; Johnson et al., 2000b, Washington et al., 2000).”
BackgroundRev1 Employs a Novel Mechanism of DNA Synthesis Using a Protein Template
Nair, D. T., Johnson, R. E., Prakash, L., Prakash, S., & Aggarwal, A. K. (2005) · Science · doi:10.1126/science.1116336 · Cited by 249
Self-citationPhysical and Functional Interactions of Human DNA Polymerase η with PCNA
Haracska, L., Johnson, R. E., Unk, I., Phillips, B., Hurwitz, J., Prakash, L., & Prakash, S. (2001) · Molecular and Cellular Biology · doi:10.1128/mcb.21.21.7199-7206.2001 · Cited by 235
“Both yeast Pol and hPol are low-processivity enzymes, incorporating only a few nucleotides per DNA binding event (21, 38 ).”
BackgroundSelf-citationThe many faces of DNA polymerases: Strategies for mutagenesis and for mutational avoidance
Friedberg, E. C., Feaver, W. J., & Gerlach, V. L. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.120152397 · Cited by 222
Vaisman, A., Masutani, C., Hanaoka, F., & Chaney, S. G. (2000) · Biochemistry · doi:10.1021/bi000130k · Cited by 212
The Translesion DNA Polymerase ζ Plays a Major Role in Ig and bcl-6 Somatic Hypermutation
Zan, H., Komori, A., Li, Z., Cerutti, A., Schaffer, A., Flajnik, M. F., Diaz, M., & Casali, P. (2001) · Immunity · doi:10.1016/s1074-7613(01)00142-x · Cited by 204
“In spite of its highly distributive nature and intrinsic lack of proofreading capacity, pol η bypasses UV- and chemical-induced lesions by inserting deoxynucleotides mostly in an error-free fashion in yeast, mouse, and human cells, as it favors the intervention of exogenous 3′ → 5′ exonucleases (Johnson et al., 1999a,…”
BackgroundInfluentialPreferential Incorporation of G Opposite Template T by the Low-Fidelity Human DNA Polymerase ι
Zhang, Y., Yuan, F., Wu, X., & Wang, Z. (2000) · Molecular and Cellular Biology · doi:10.1128/mcb.20.19.7099-7108.2000 · Cited by 200
“To obtain a quantitative measurement of base selections by human Poli, we measured its nucleotide incorporation fidelity using a steady-state kinetic analysis (3, 36).”
MethodRole of DNA Polymerase η in the Bypass of a (6-4) TT Photoproduct
Johnson, R. E., Haracska, L., Prakash, S., & Prakash, L. (2001) · Molecular and Cellular Biology · doi:10.1128/mcb.21.10.3558-3563.2001 · Cited by 199
Self-citationInteraction with PCNA Is Essential for Yeast DNA Polymerase η Function
Haracska, L., Kondratick, C. M., Unk, I., Prakash, S., & Prakash, L. (2001) · Molecular Cell · doi:10.1016/s1097-2765(01)00319-7 · Cited by 198
Self-citationError-prone lesion bypass by human DNA polymerase eta
Zhang, Y. (2000) · Nucleic Acids Research · doi:10.1093/nar/28.23.4717 · Cited by 188
The human DINB1 gene encodes the DNA polymerase Polθ
Johnson, R. E., Prakash, S., & Prakash, L. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.8.3838 · Cited by 182
Self-citationMcDonald, J. P., Frank, E. G., Plosky, B. S., Rogozin, I. B., Masutani, C., Hanaoka, F., Woodgate, R., & Gearhart, P. J. (2003) · The Journal of Experimental Medicine · doi:10.1084/jem.20030767 · Cited by 172
Error rate and specificity of human and murine DNA polymerase η
Matsuda, T., Bebenek, K., Masutani, C., Rogozin, I. B., Hanaoka, F., & Kunkel, T. A. (2001) · Journal of Molecular Biology · doi:10.1006/jmbi.2001.4937 · Cited by 170
The expanding polymerase universe
Goodman, M. F., & Tippin, B. L. (2000) · Nature Reviews Molecular Cell Biology · doi:10.1038/35040051 · Cited by 166
Human DINB1 -encoded DNA polymerase κ is a promiscuous extender of mispaired primer termini
Washington, M. T., Johnson, R. E., Prakash, L., & Prakash, S. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.032594399 · Cited by 165
Self-citationYeast Rev1 Protein Is a G Template-specific DNA Polymerase
Haracska, L., Prakash, S., & Prakash, L. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m112146200 · Cited by 164
Self-citationReplication past O 6 -Methylguanine by Yeast and Human DNA Polymerase η
Haracska, L., Prakash, S., & Prakash, L. (2000) · Molecular and Cellular Biology · doi:10.1128/mcb.20.21.8001-8007.2000 · Cited by 142
“We have previously suggested that Polh has a flexible active site which renders the enzyme more tolerant of DNA distortions, enabling it to synthesize DNA past a T-T dimer (17, 44, 45 ).”
BackgroundSelf-citationRecent developments in the optimization of thermostable DNA polymerases for efficient applications
Pavlov, A. R., Pavlova, N. V., Kozyavkin, S. A., & Slesarev, A. I. (2004) · Trends in biotechnology · doi:10.1016/j.tibtech.2004.02.011 · Cited by 142
Acharya, N., Johnson, R. E., Prakash, S., & Prakash, L. (2006) · Molecular and Cellular Biology · doi:10.1128/mcb.01671-06 · Cited by 128
“Thus, Pol’s rate of misincorporation is 104 (10, 12, 19), compared to Pol, which misincorporates dNTPs with a frequency of 102 ( 39 ).”
BackgroundSelf-citationKokoska, R. J., McCulloch, S. D., & Kunkel, T. A. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m308515200 · Cited by 125
Barbour, L., & Xiao, W. (2003) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/j.mrfmmm.2003.08.014 · Cited by 125
Yeast DNA Polymerase η Utilizes an Induced-Fit Mechanism of Nucleotide Incorporation
Washington, M. T., Prakash, L., & Prakash, S. (2001) · Cell · doi:10.1016/s0092-8674(01)00613-4 · Cited by 123
Self-citationHarfe, B. D., & Jinks-Robertson, S. (2000) · Molecular Cell · doi:10.1016/s1097-2765(00)00145-3 · Cited by 122
Biochemical, Structural, and Physiological Characterization of Terminal Deoxynucleotidyl Transferase
Fowler, J. D., & Suo, Z. (2006) · Chemical Reviews · doi:10.1021/cr040445w · Cited by 119
Pre-Steady-State Kinetic Studies of the Fidelity of Sulfolobus solfataricus P2 DNA Polymerase IV
Fiala, K. A., & Suo, Z. (2004) · Biochemistry · doi:10.1021/bi0357457 · Cited by 115
Washington, M. T., Minko, I. G., Johnson, R. E., Wolfle, W. T., Harris, T. M., Lloyd, R. S., Prakash, S., & Prakash, L. (2004) · Molecular and Cellular Biology · doi:10.1128/mcb.24.13.5687-5693.2004 · Cited by 114
“Pol, which exhibit nearly similar efficiencies and fidelities of nucleotide incorporation opposite each of the four template bases (17, 19, 44 ), Pol is unusual in that the efficiency and fidelity of nucleotide incorporation by this polymerase is dependent on the identity of the template base.”
BackgroundSelf-citationCrystal Structure of the Catalytic Core of Human DNA Polymerase Kappa
Uljon, S., Johnson, R. E., Edwards, T. A., Prakash, S., Prakash, L., & Aggarwal, A. K. (2004) · Structure · doi:10.1016/j.str.2004.05.011 · Cited by 114
“The total buried surface area between the to 10 3 for Pol (Johnson et al. 2000a, 2000c; Washington et al. 1999).”
BackgroundSelf-citationBerdis, A. J. (2009) · Chemical Reviews · doi:10.1021/cr800530b · Cited by 113
Human DNA polymerase kappa synthesizes DNA with extraordinarily low fidelity
Zhang, Y. (2000) · Nucleic Acids Research · doi:10.1093/nar/28.21.4147 · Cited by 109
Molecular dissection of the domain architecture and catalytic activities of human PrimPol
Keen, B. A., Jóźwiakowski, S. K., Bailey, L. J., Bianchi, J., & Doherty, A. J. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku214 · Cited by 114
“This distributive character of PrimPol is reminiscent of the DNA polymerase processivity reported for the Y-family TLS polymerases, such as Pol and , which predominantly incorporate up to 6 or 7 nucleotides (16,29).”
BackgroundMethodSloppier copier DNA polymerases involved in genome repair
Goodman, M. F., & Tippin, B. L. (2000) · Current Opinion in Genetics & Development · doi:10.1016/s0959-437x(00)00057-5 · Cited by 107
Parker, J. L., Bielen, A. B., Đikić, I., & Ulrich, H. D. (2007) · Nucleic Acids Research · doi:10.1093/nar/gkl1102 · Cited by 108
Regulated Proteolysis of DNA Polymerase η during the DNA-Damage Response in C. elegans
Kim, S.-H., & Michael, W. M. (2008) · Molecular Cell · doi:10.1016/j.molcel.2008.11.016 · Cited by 99
“…polymerase after TLS. Current models for TLS suggest that post-TLS exchange occurs because pol h, which exhibits low processivity in vitro (Washington et al., 1999; McCulloch et al., 2004, 2007), simply disassociates from the template after TLS, and this allows the replicative polymerase…”
BackgroundTranslesion synthesis by the UmuC family of DNA polymerases
Wang, Z. (2001) · Mutation Research/DNA Repair · doi:10.1016/s0921-8777(01)00089-1 · Cited by 97
Guo, D. (2001) · Nucleic Acids Research · doi:10.1093/nar/29.13.2875 · Cited by 98
“Kinetic analysis of primer extension by yeast Polζ was performed using a previously described method (30,40,41).”
MethodBergoglio, V., Canitrot, Y., Hogarth, L., Minto, L., Howell, S. B., Cazaux, C., & Hoffmann, J. (2001) · Oncogene · doi:10.1038/sj.onc.1204743 · Cited by 92
“New cellular error-prone DNA polymerases have been recently described (Matsuda et al., 2000; Washington et al., 1999) which belong to the superfamily of DNA polymerases that are able to bypass lesions in DNA (Woodgate, 1999).”
BackgroundCoping with replication ‘train wrecks’ in Escherichia coli using Pol V, Pol II and RecA proteins
Goodman, M. F. (2000) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(00)01564-4 · Cited by 107
Variations on a theme: Eukaryotic Y-family DNA polymerases
Washington, M. T., Carlson, K. D., Freudenthal, B., & Pryor, J. M. (2009) · Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics · doi:10.1016/j.bbapap.2009.07.004 · Cited by 90
“Steady state kinetic studies as well as other complementary approaches have shown that purified yeast andmammalianpolη synthesizeDNAwith a low fidelity with error frequencies ranging from 10 to 10 [30,31,42–44].”
BackgroundSelf-citationReconstitution of translesion synthesis reveals a mechanism of eukaryotic DNA replication restart
Guilliam, T. A., & Yeeles, J. T. P. (2020) · Nature Structural & Molecular Biology · doi:10.1038/s41594-020-0418-4 · Cited by 82
Xeroderma pigmentosum genes: Functions inside and outside DNA repair
Sugasawa, K. (2007) · Carcinogenesis · doi:10.1093/carcin/bgm282 · Cited by 83
“This seems to be regulated at several levels, including low processivity of the enzyme itself (150, 164), altered binding affinities for different template-primer structures (165), and interaction with a monoubiquitylated PCNA clamp (166, 167).”
BackgroundChan, K., Resnick, M. A., & Gordenin, D. A. (2013) · DNA repair · doi:10.1016/j.dnarep.2013.07.008 · Cited by 83
Kozmin, S. G. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg489 · Cited by 78
Eoff, R. L., Irimia, A., Angel, K. C., Egli, M., & Guengerich, F. P. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m702290200 · Cited by 75
Mechanism of nucleotide incorporation opposite a thymine-thymine dimer by yeast DNA polymerase η
Washington, M. T., Prakash, L., & Prakash, S. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2134223100 · Cited by 76
Self-citationRequirement of Watson-Crick Hydrogen Bonding for DNA Synthesis by Yeast DNA Polymerase η
Washington, M. T., Helquist, S. A., Kool, E. T., Prakash, L., & Prakash, S. (2003) · Molecular and Cellular Biology · doi:10.1128/mcb.23.14.5107-5112.2003 · Cited by 75
“This is because for certain base pairs, Pol preferentially incorporates the correct nucleotide several thousandfold more efficiently than the incorrect nucleotide ( 45 ) and because Pol requires minor-groove interactions between the protein and the incoming nucleotide substrate (47).”
BackgroundInfluentialSelf-citationMadia, F., Wei, M., Yuan, V., Hu, J. H., Gattazzo, C., Pham, P., Goodman, M. F., & Longo, V. D. (2009) · The Journal of Cell Biology · doi:10.1083/jcb.200906011 · Cited by 72
“…show that age-dependent mutagenesis was reduced in cells lacking either REV3 or REV7, the two subunits of Pol polymerase, but was increased in mutants lacking RAD30, which is regarded as an error-free DNA damage bypass enzyme (Johnson et al., 1999; Washington et al., 1999; Prakash et al., 2000).”
BackgroundInfluentialAccuracy of lesion bypass by yeast and human DNA polymerase η
Washington, M. T., Johnson, R. E., Prakash, L., & Prakash, S. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.121007298 · Cited by 71
Self-citationReconstitution of DNA repair synthesis in vitro and the role of polymerase and helicase activities
Šebesta, M., Burkovics, P., Haracska, L., & Krejčí, L. (2011) · DNA repair · doi:10.1016/j.dnarep.2011.03.003 · Cited by 69
DNA polymerase iota and related Rad30–like enzymes
McDonald, J. P., Tissier, A., Frank, E. G., Iwai, S., Hanaoka, F., & Woodgate, R. (2001) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2000.0748 · Cited by 68
Helix–hairpin–helix motifs confer salt resistance and processivity on chimeric DNA polymerases
Pavlov, A. R., Belova, G. I., Kozyavkin, S. A., & Slesarev, A. I. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.202127199 · Cited by 66
Carlson, K. D., & Washington, M. T. (2005) · Molecular and Cellular Biology · doi:10.1128/mcb.25.6.2169-2176.2005 · Cited by 67
“In Saccharomyces cerevisiae, disruption of the RAD30 gene encoding Pol results in increased sensitivity to UV irradiation and an increase in the number of UVinduced mutations ( 21 , 30, 37).”
BackgroundInfluentialSelf-citationProcessivity of Ribozyme-Catalyzed RNA Polymerization
Lawrence, M. S., & Bartel, D. P. (2003) · Biochemistry · doi:10.1021/bi034228l · Cited by 62
“9, placing it among lowprocessivity proteinaceous polymerases such as yeast polymeraseη (12).”
Enhanced Genetic Integrity in Mouse Germ Cells1
Murphey, P., McLean, D. J., McMahan, C. A., Walter, C. A., & McCarrey, J. R. (2012) · Biology of Reproduction · doi:10.1095/biolreprod.112.103481 · Cited by 57
Franklin, A., Milburn, P. J., Blanden, R. V., & Steele, E. J. (2004) · Immunology and Cell Biology · doi:10.1046/j.0818-9641.2004.01221.x · Cited by 56
Washington, M. T., Johnson, R. E., Prakash, L., & Prakash, S. (2003) · Molecular and Cellular Biology · doi:10.1128/mcb.24.2.936-943.2004 · Cited by 55
“Pol from both yeast and humans synthesizes DNA with a low fidelity, misincorporating nucleotides with frequencies of 10 2 to 10 3 (11, 14, 19).”
BackgroundSelf-citationEvidence for a Watson-Crick Hydrogen Bonding Requirement in DNA Synthesis by Human DNA Polymerase κ
Wolfle, W. T., Washington, M. T., Kool, E. T., Spratt, T. E., Helquist, S. A., Prakash, L., & Prakash, S. (2005) · Molecular and Cellular Biology · doi:10.1128/mcb.25.16.7137-7143.2005 · Cited by 55
“For example, both yeast and human Pol misincorporate nucleotides with a frequency of 102 to 103, and both of these enzymes replicate through a cis-syn TT dimer by inserting two A’s opposite the two T’s of the dimer with the same efficiency and fidelity as opposite the undamaged T’s (12, 40, 41 , 43).”
BackgroundSelf-citationWhy Cockayne syndrome patients do not get cancer despite their DNA repair deficiency
Reid-Bayliss, K. S., Arron, S. T., Loeb, L. A., Bezrookove, V., & Cleaver, J. E. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1610020113 · Cited by 55
Mismatch Extension Ability of Yeast and Human DNA Polymerase η
Washington, M. T., Johnson, R. E., Prakash, S., & Prakash, L. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m009049200 · Cited by 54
Self-citationBellows, A. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg811 · Cited by 53
Lehmann, A. R. (2000) · Gene · doi:10.1016/s0378-1119(00)00250-x · Cited by 52
Involvement of DNA polymerase β in DNA replication and mutagenic consequences
Servant, L., Bieth, A., Hayakawa, H., Cazaux, C., & Hoffmann, J. (2002) · Journal of Molecular Biology · doi:10.1006/jmbi.2001.5307 · Cited by 53
Skoneczna, A., McIntyre, J., Skoneczny, M., Policińska, Z., & Śledziewska-Gójska, E. (2006) · Journal of Molecular Biology · doi:10.1016/j.jmb.2006.11.093 · Cited by 53
Monitoring Processivity and Length Control of a Carbohydrate Polymerase
Levengood, M. R., Splain, R. A., & Kiessling, L. L. (2011) · Journal of the American Chemical Society · doi:10.1021/ja204448t · Cited by 51
Seo, K. Y., Nagalingam, A., Miri, S., Yin, J., Chandani, S., Kolbanovskiy, A., Shastry, A., & Loechler, E. L. (2006) · DNA repair · doi:10.1016/j.dnarep.2005.12.009 · Cited by 51
Base Pairing and Replicative Processing of the Formamidopyrimidine-dG DNA Lesion
Ober, M. S., Müller, H., Pieck, C., Gierlich, J., & Carell, T. (2005) · Journal of the American Chemical Society · doi:10.1021/ja0549188 · Cited by 50
Pavlov, Y. I., Nguyen, D., & Kunkel, T. A. (2001) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/s0027-5107(01)00131-2 · Cited by 50
Degradation or Maintenance: Actions of the Ubiquitin System on Eukaryotic Chromatin
Ulrich, H. D. (2002) · Eukaryotic Cell · doi:10.1128/ec.1.1.1-10.2002 · Cited by 48
“However, the overall fidelity of Pol on nondamaged templates is much lower than that of replicative polymerases (128).”
BackgroundĆejka, P., Vondrejs, V., & Štorchová, Z. (2001) · Genetics · doi:10.1093/genetics/159.3.953 · Cited by 45
Acidic Residues Critical for the Activity and Biological Function of Yeast DNA Polymerase η
Kondratick, C. M., Washington, M. T., Prakash, S., & Prakash, L. (2001) · Molecular and Cellular Biology · doi:10.1128/mcb.21.6.2018-2025.2001 · Cited by 46
“relative insensitivity of Polh to geometric distortions in DNA may derive from a flexible active site able to conform to distortions in Watson-Crick geometry (8, 17, 31, 32 ).”
BackgroundSelf-citationY-Family Polymerase Conformation Is a Major Determinant of Fidelity and Translesion Specificity
Wilson, R. C., Jackson, M. A., & Pata, J. D. (2012) · Structure · doi:10.1016/j.str.2012.11.005 · Cited by 45
“The importance of these polymerases became apparent when the variant form of xeroderma pigmentosum (XPV) was found to be caused by a lack of polymerase (pol) eta, which was shown to have the ability to bypass cis-syn cyclopyrimidine dimers (CPD) accurately (McDonald et al., 1999; Washington et al., 1999).”
BackgroundWashington, M. T., Johnson, R. E., Prakash, L., & Prakash, S. (2003) · Molecular and Cellular Biology · doi:10.1128/mcb.23.22.8316-8322.2003 · Cited by 45
“Pol can also efficiently replicate through other DNA lesions, such as 8-oxoguanine and O6-methylguanine (4, 5). We previously suggested (as determined on the basis of the proficient ability of Pol to replicate through distorting DNA lesions) that Pol possesses an active site that is remarkably tolerant of geometric di…”
BackgroundInfluentialSelf-citationWolfle, W. T., Johnson, R. E., Minko, I. G., Lloyd, R. S., Prakash, S., & Prakash, L. (2005) · Molecular and Cellular Biology · doi:10.1128/mcb.25.19.8748-8754.2005 · Cited by 45
“Similar to high-fidelity replicative polymerases, Pols and incorporate nucleotides opposite the four template bases with nearly equivalent efficiencies and fidelities (6, 8, 23), while Pol incorporates nucleotides opposite the four template bases with very different efficiencies and fidelities (2, 7, 19, 21).”
BackgroundSelf-citationGarcía‐Ortiz, M. V., Ariza, R. R., Hoffman, P. D., Hays, J. B., & Roldán‐Arjona, T. (2004) · The Plant Journal · doi:10.1111/j.1365-313x.2004.02112.x · Cited by 45
Song, L., Chaudhuri, M. M., Knopf, C. W., & Parris, D. S. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m309848200 · Cited by 43
Human PrimPol mutation associated with high myopia has a DNA replication defect
Keen, B. A., Bailey, L. J., Jóźwiakowski, S. K., & Doherty, A. J. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku879 · Cited by 43
“The PrimPol variants were analysed for processivity as described previously (16).”
MethodAbdulovic, A. L., & Jinks-Robertson, S. (2005) · Genetics · doi:10.1534/genetics.105.052480 · Cited by 43
“In spite of its original designation as an error-free TLS polymerase, Polh has very low fidelity when copying undamaged DNA templates (Washington et al.1999) and is important for UV-induced mutagenesis in at least some yeast assays (Zhang and Siede 2002).”
BackgroundHaracska, L., Prakash, L., & Prakash, S. (2003) · Genes & Development · doi:10.1101/gad.1146103 · Cited by 41
“Steady-state kinetic analyses have indicated that Pol and Pol misincorporate nucleotides with a frequency of ∼10−2 to 10−3 and ∼10−3 to 10−4, respectively (Washington et al. 1999; Johnson et al. 2000a,c).”
BackgroundSelf-citationMutagenic and Nonmutagenic Bypass of DNA Lesions byDrosophila DNA Polymerases dpolη and dpolι
Ishikawa, T., Uematsu, N., Mizukoshi, T., Iwai, S., Iwasaki, H., Masutani, C., Hanaoka, F., Ueda, R., Ohmori, H., & Todo, T. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m009822200 · Cited by 37
Zhang, H. (2002) · Nucleic Acids Research · doi:10.1093/nar/30.5.1262 · Cited by 38
Minko, I. G., Washington, M. T., Prakash, L., Prakash, S., & Lloyd, R. S. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m007867200 · Cited by 37
Self-citationDieckman, L. M., Johnson, R. E., Prakash, S., & Washington, M. (2010) · Biochemistry · doi:10.1021/bi100556m · Cited by 37
“translesion synthesis polymerases such as pol ζ [45], pol η [46,47], pol κ [48], and pol ι [45,49,50], which have error rates as high as 101 for pol ι and as low as 10−4 for pol ζ and pol κ.”
BackgroundSelf-citationWashington, M. T., Wolfle, W. T., Spratt, T. E., Prakash, L., & Prakash, S. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0837578100 · Cited by 35
Self-citationThe Y-Family DNA Polymerase Dpo4 Uses a Template Slippage Mechanism To Create Single-Base Deletions
Wu, Y., Wilson, R. C., & Pata, J. D. (2011) · Journal of Bacteriology · doi:10.1128/jb.00012-11 · Cited by 34
“The Y-family of DNA polymerases was defined in 2001 (21), following the discovery that the gene responsible for the variant form of xeroderma pigmentosum encodes polymerase (Pol) eta, the seventh human polymerase identified (19, 30).”
BackgroundRepair of Oxidative DNA Damage in Saccharomyces cerevisiae
Chalissery, J., Jalal, D., Natour, Z. A., & Hassan, A. H. (2017) · DNA repair · doi:10.1016/j.dnarep.2016.12.010 · Cited by 37
Regulatory Role of Ubiquitin in Eukaryotic DNA Translesion Synthesis
Yang, K., Weinacht, C. P., & Zhuang, Z. (2013) · Biochemistry · doi:10.1021/bi400194r · Cited by 35
Metadata
- Authors
- Washington, M. T., Johnson, R. E., Prakash, S., & Prakash, L.
- Affiliations
- The University of Texas Medical Branch at Galveston (US)
- Corresponding author
- Prakash, Louise
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1999-12
- Volume
- 274
- Issue
- 52
- Pages
- 36835-36838
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2107896468
- Cited by
- 181
- References
- 26
- Citations by year
- 2026: 2 · 2025: 2 · 2023: 2 · 2021: 4 · 2020: 3 · 2019: 1 · 2018: 1 · 2017: 4 · 2016: 4 · 2015: 1 · 2014: 5 · 2013: 6 · 2012: 8
- FWCI
- 6.50
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01gm019261-30)
- National Institute of General Medical Sciences (GM-19261)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- DNA polymerase eta, UV-damaged DNA, translesion synthesis, polymerase fidelity, polymerase processivity, active site flexibility, Saccharomyces cerevisiae
- MeSH terms
- DNA Polymerase iota, Y-Family DNA Polymerases, DNA Damage, DNA-Directed DNA Polymerase, DNA Replication, Kinetics, Mutation, Saccharomyces cerevisiae
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
