CoChat
Sign Up
Cited by 702

The DOI matches its Crossref record.

RAS/ERK Signaling Promotes Site-specific Ribosomal Protein S6 Phosphorylation via RSK and Stimulates Cap-dependent Translation

Roux, Philippe P., Shahbazian, David, Vu, Hieu, Holz, Marina K., Cohen, Michael S., Taunton, Jack, Sonenberg, Nahum, Blenis, John·2007·Journal of Biological Chemistry
DOI 10.1074/jbc.m700906200
Open this paper in CoChat — free account

Abstract

Converging signals from the mammalian target of rapamycin (mTOR) and phosphoinositide 3-kinase (PI3K) pathways are well established to modulate translation initiation. Less is known regarding the molecular basis of protein synthesis regulated by other inputs, such as agonists of the Ras/extracellular signal-regulated kinase (ERK) signaling cascade. Ribosomal protein (rp) S6 is a component of the 40S ribosomal subunit that becomes phosphorylated at several serine residues upon mitogen stimulation, but the exact molecular mechanisms regulating its phosphorylation and the function of phosphorylated rpS6 is poorly understood. Here, we provide evidence that activation of the p90 ribosomal S6 kinases (RSKs) by serum, growth factors, tumor promoting phorbol esters, and oncogenic Ras is required for rpS6 phosphorylation downstream of the Ras/ERK signaling cascade. We demonstrate that while ribosomal S6 kinase 1 (S6K1) phosphorylates rpS6 at all sites, RSK exclusively phosphorylates rpS6 at Ser(235/236) in vitro and in vivo using an mTOR-independent mechanism. Mutation of rpS6 at Ser(235/236) reveals that phosphorylation of these sites promotes its recruitment to the 7-methylguanosine cap complex, suggesting that Ras/ERK signaling regulates assembly of the translation preinitiation complex. These data demonstrate that RSK provides an mTOR-independent pathway linking the Ras/ERK signaling cascade to the translational machinery.

Overview

Integrity signals

Registry

Crossref · 10.1074/jbc.m700906200

Cites

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 39

  1. Dever, 2002, Cell, 108, 545, 10.1016/S0092-8674(02)00642-6 · DOI
  2. Clemens, 2004, Oncogene, 23, 3180, 10.1038/sj.onc.1207544 · DOI
  3. Richter, 2005, Nature, 433, 477, 10.1038/nature03205 · DOI
  4. Hay, 2004, Genes Dev., 18, 1926, 10.1101/gad.1212704 · DOI · Paper page
  5. Fingar, 2004, Oncogene, 23, 3151, 10.1038/sj.onc.1207542 · DOI
  6. Richardson, 2004, Semin. Cell Dev. Biol., 15, 147, 10.1016/j.semcdb.2003.12.023 · DOI
  7. Gingras, 2004, Curr. Top Microbiol. Immunol., 279, 169
  8. Averous, 2006, Oncogene, 25, 6423, 10.1038/sj.onc.1209887 · DOI
  9. Ruvinsky, 2006, Trends Biochem. Sci., 31, 342, 10.1016/j.tibs.2006.04.003 · DOI
  10. Thomas, 1979, Proc. Natl. Acad. Sci. U. S. A., 76, 3952, 10.1073/pnas.76.8.3952 · DOI
  11. Gressner, 1974, J. Biol. Chem., 249, 6917, 10.1016/S0021-9258(19)42145-5 · DOI
  12. Bandi, 1993, J. Biol. Chem., 268, 4530, 10.1016/S0021-9258(18)53642-5 · DOI
  13. Krieg, 1988, J. Biol. Chem., 263, 11473, 10.1016/S0021-9258(18)37981-X · DOI
  14. Roux, 2004, Microbiol. Mol. Biol. Rev., 68, 320, 10.1128/MMBR.68.2.320-344.2004 · DOI
  15. Hauge, 2006, J. Cell Sci., 119, 3021, 10.1242/jcs.02950 · DOI
  16. Chung, 1992, Cell, 69, 1227, 10.1016/0092-8674(92)90643-Q · DOI
  17. Blenis, 1991, Cell Growth Differ., 2, 279
  18. Lee-Fruman, 1999, Oncogene, 18, 5108, 10.1038/sj.onc.1202894 · DOI
  19. Pende, 2004, Mol. Cell Biol., 24, 3112, 10.1128/MCB.24.8.3112-3124.2004 · DOI
  20. Volarevic, 2000, Science, 288, 2045, 10.1126/science.288.5473.2045 · DOI
  21. Nygard, 1990, J. Biol. Chem., 265, 6030, 10.1016/S0021-9258(19)39286-5 · DOI
  22. Burkhard, 1983, J. Biol. Chem., 258, 14003, 10.1016/S0021-9258(17)44016-6 · DOI
  23. Ruvinsky, 2005, Genes Dev., 19, 2199, 10.1101/gad.351605 · DOI
  24. Roux, 2004, Proc. Natl. Acad. Sci. U. S. A., 101, 13489, 10.1073/pnas.0405659101 · DOI
  25. Kruger, 2001, Mol. Cell Biol., 21, 8357, 10.1128/MCB.21.24.8357-8364.2001 · DOI
  26. Roux, 2003, Mol. Cell Biol., 23, 4796, 10.1128/MCB.23.14.4796-4804.2003 · DOI
  27. Cohen, 2005, Science, 308, 1318, 10.1126/science1108367 · DOI
  28. Anjum, 2005, Curr. Biol., 15, 1762, 10.1016/j.cub.2005.08.050 · DOI
  29. Shahbazian, 2006, EMBO J., 25, 2781, 10.1038/sj.emboj.7601166 · DOI
  30. Tee, 2003, Curr. Biol., 13, 1259, 10.1016/S0960-9822(03)00506-2 · DOI · Paper page
  31. Romanelli, 1999, Mol. Cell Biol., 19, 2921, 10.1128/MCB.19.4.2921 · DOI
  32. Leighton, 1995, FEBS Lett., 375, 289, 10.1016/0014-5793(95)01170-J · DOI
  33. Richards, 2001, Mol. Cell Biol., 21, 7470, 10.1128/MCB.21.21.7470-7480.2001 · DOI
  34. Holz, 2005, Cell, 123, 569, 10.1016/j.cell.2005.10.024 · DOI · Paper page
  35. Erikson, 1985, Proc. Natl. Acad. Sci. U. S. A., 82, 742, 10.1073/pnas.82.3.742 · DOI
  36. Ballou, 1991, Nature, 349, 348, 10.1038/349348a0 · DOI
  37. Ma, 2005, Cell, 121, 179, 10.1016/j.cell.2005.02.031 · DOI · Paper page
  38. Rajasekhar, 2003, Mol. Cell, 12, 889, 10.1016/S1097-2765(03)00395-2 · DOI · Paper page
  39. Kelleher, 2004, Neuron, 44, 59, 10.1016/j.neuron.2004.09.013 · DOI · Paper page

Cited by

Sorted by citations

Metadata

Authors
Roux, P. P., Shahbazian, D., Vu, H., Holz, M. K., Cohen, M. S., Taunton, J., Sonenberg, N., & Blenis, J.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2007-05
Volume
282
Issue
19
Pages
14056-14064
ISSN
0021-9258
DOI
10.1074/jbc.m700906200
Cited by
702
References
0
Language
en
Licence

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review