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Cholesterol determination in high-density lipoproteins separated by three different methods.
Abstract
We describe a simplified method for measuring high-density lipoprotein cholesterol in serum after very-low- and low-density lipoproteins have been precipitated from the specimen with sodium phosphotungstate and Mg2+. Values so obtained correlate well with values obtained with the heparin-Mn2+ precipitation technique (r = 0.95, CV less than 5% in 66% of the subjects studied and between 5 and 10% in the remaining ones) or by ultracentrifugal separation (r = 0.82, CV less than 5% in 80% of the subjects studied and between 5 and 10% in the remaining ones). Our precipitation technique is more appropriate for routine clinical laboratory use.
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Metadata
- Authors
- Lopes-Virella, M. F., Stone, P., Ellis, S., & Colwell, J. A.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1977-05-01
- Volume
- 23
- Issue
- 5
- Pages
- 882-884
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W2127258766
- Cited by
- 1,841
- References
- 0
- FWCI
- 21.55
- Type
- journal article
- Language
- en
- Open access
- closed
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