Identification and validation of novel serum markers for early diagnosis of endometriosis
Abstract
BACKGROUND: Non-invasive diagnosis of endometriosis is urgently required to prevent the long delay between the onset of symptoms and diagnosis. A biomarker that possesses both high sensitivity and specificity is greatly required. Here, we describe the use of a proteomic approach to identify potential novel endometrial antigens using sera from endometriosis patients and healthy controls, with evaluation of biomarkers for non-invasive diagnosis of endometriosis. METHODS: A cross-sectional study was conducted to identify specific endometrial antigens using 1D and 2D western blots in women with early endometriosis (n = 17), advanced endometriosis (n = 23) and without endometriosis (n = 30). Five immunoreactive spots were analyzed using matrix-assisted laser desorption/ionization-time-of-flight/mass spectrometry with MASCOT analysis. ELISAs were established for specific epitopes and autoantibody titres were estimated in an independent cohort comprising women with early endometriosis (n = 18), advanced endometriosis (n = 32) and without endometriosis (n = 27) for validation. RESULTS: The 2D western blot analysis resulted in the identification of three endometrial antigens, tropomyosin 3 (TPM3), stomatin-like protein 2 (SLP2) and tropomodulin 3 (TMOD3). Serum levels of antibodies against the epitopes from the immunodominant region of proteins TPM3, SLP2 and TMOD3 were significantly elevated in endometriosis patients when compared with controls. Sensitivity and specificity of serum anti-TPM3a-autoAb (61%, 93%), anti-TPM3c-autoAb (44%, 93%), anti-TPM3d-autoAb (78%, 89%), anti-SLP2a-autoAb (50%, 96%), anti-SLP2c-autoAb (61%, 93%), anti-TMOD3b-autoAb (61%, 96%), serum anti-TMOD3c-autoAb (78%, 93%) and anti-TMOD3d-autoAb (78%, 96%) were better than those of serum CA125 levels (21%, 89%) in the detection of early stages of endometriosis. CONCLUSIONS: Serum anti-TPM3a-autoAb, anti-TPM3c-autoAb, anti-TPM3d-autoAb, anti-SLP2a-autoAb, anti-SLP2c-autoAb, anti-TMOD3b-autoAb, anti-TMOD3c-autoAb and anti-TMOD3d-autoAb could be new markers for the early diagnosis of endometriosis.
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Autoantibodies against different epitopes of TPM3, SLP2, and TMOD3 were significantly elevated in serum of endometriosis patients with both minimal/mild and moderate/severe disease.
“Autoantibodies against different epitopes of tropomyosin 3 (TPM3), stomatin-like protein 2 (SLP2), and tropomodulin 3 (TMOD3) were significantly elevated in the serum of endometriosis patients with both minimal/mild and moderate/severe disease [80].”
From Update on biomarkers for the detection of endometriosis (2015) · cites this paper as [80]
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Update on biomarkers for the detection of endometriosis
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“Autoantibodies against different epitopes of tropomyosin 3 (TPM3), stomatin-like protein 2 (SLP2), and tropomodulin 3 (TMOD3) were significantly elevated in the serumof endometriosis patients with bothminimal/mild and moderate/severe disease [80].”
Diagnostic accuracy of cancer antigen 125 for endometriosis: A systematic review and meta-analysis
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- Authors
- Gajbhiye, R., Sonawani, A., Khan, S., Suryawanshi, A., Kadam, S., Warty, N., Raut, V., & Khole, V.
- Affiliations
- ICMR National Institute for Research on Women's Health (IN)
- Institute of Life Sciences (IN)
- Corresponding author
- Khole, V.
- Venue
- Human Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-12-08
- Volume
- 27
- Issue
- 2
- Pages
- 408-417
- ISSN
- 0268-1161 (ISSN-L)
- 1460-2350
- OpenAlex
- W2165441271
- Cited by
- 53
- References
- 46
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 3 · 2023: 2 · 2022: 5 · 2021: 3 · 2020: 3 · 2019: 5 · 2018: 2 · 2017: 3 · 2016: 6 · 2015: 3 · 2014: 4 · 2013: 6
- FWCI
- 3.59
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Human Reproduction (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Indian Council of Medical Research
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Ectopic Pregnancy Diagnosis and Management
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Keywords
- endometriosis, serum biomarkers, autoantibodies, early diagnosis, non-invasive diagnosis, TPM3, STOML2, CA125, proteomics, ELISA validation, sensitivity and specificity
- MeSH terms
- Adult, Antibody Specificity, Autoantibodies, Autoantigens, Blood Proteins, Cross-Sectional Studies, Endometriosis, Female, Humans, Membrane Proteins, Peptide Fragments, Peptide Mapping, Sensitivity and Specificity, Severity of Illness Index, Tropomyosin, Cohort Studies, Biomarkers, Immunodominant Epitopes, Early Diagnosis, Tropomodulin, Young Adult
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